Protein : Qrob_P0260490.2 Q. robur

Protein Identifier  ? Qrob_P0260490.2 Organism . Name  Quercus robur
Score  97.0 Score Type  egn
Protein Description  (M=1) K03138 - transcription initiation factor TFIIF subunit alpha Code Enzyme  EC:3.4.19.12, EC:3.4.22.69
Gene Prediction Quality  validated Protein length 

Sequence

Length: 555  
Kegg Orthology  K03138

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0 Synonyms

9 GO Terms

Identifier Name Description
GO:0003677 DNA binding Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid).
GO:0009522 photosystem I A photosystem that contains an iron-sulfur reaction center associated with accessory pigments and electron carriers. In cyanobacteria and chloroplasts, photosystem I functions as a light-dependent plastocyanin-ferredoxin oxidoreductase, transferring electrons from plastocyanin to ferredoxin; in photosynthetic bacteria that have only a single type I photosystem, such as the green sulfur bacteria, electrons can go either to ferredoxin (Fd) -> NAD+ or to menaquinone (MK) -> Cytb/FeS -> Cytc555 -> photosystem I (cyclic photophosphorylation).
GO:0015979 photosynthesis The synthesis by organisms of organic chemical compounds, especially carbohydrates, from carbon dioxide (CO2) using energy obtained from light rather than from the oxidation of chemical compounds.
GO:0003824 catalytic activity Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic.
GO:0005634 nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
GO:0016021 integral component of membrane The component of a membrane consisting of the gene products and protein complexes having at least some part of their peptide sequence embedded in the hydrophobic region of the membrane.
GO:0009579 thylakoid A membranous cellular structure that bears the photosynthetic pigments in plants, algae, and cyanobacteria. In cyanobacteria thylakoids are of various shapes and are attached to, or continuous with, the plasma membrane. In eukaryotes they are flattened, membrane-bounded disk-like structures located in the chloroplasts; in the chloroplasts of higher plants the thylakoids form dense stacks called grana. Isolated thylakoid preparations can carry out photosynthetic electron transport and the associated phosphorylation.
GO:0006367 transcription initiation from RNA polymerase II promoter Any process involved in the assembly of the RNA polymerase II preinitiation complex (PIC) at an RNA polymerase II promoter region of a DNA template, resulting in the subsequent synthesis of RNA from that promoter. The initiation phase includes PIC assembly and the formation of the first few bonds in the RNA chain, including abortive initiation, which occurs when the first few nucleotides are repeatedly synthesized and then released. Promoter clearance, or release, is the transition between the initiation and elongation phases of transcription.
GO:0032968 positive regulation of transcription elongation from RNA polymerase II promoter Any process that activates or increases the frequency, rate or extent of transcription elongation, the extension of an RNA molecule after transcription initiation and promoter clearance by the addition of ribonucleotides, catalyzed by RNA polymerase II.

20 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|pop:POPTR_0006s01070g 1 554 + 554 Gaps:32 99.82 541 79.07 0.0 POPTRDRAFT_1080142 hypothetical protein
blastp_kegg lcl|rcu:RCOM_1299530 2 554 + 553 Gaps:32 99.62 533 80.04 0.0 tfiif-alpha putative
blastp_kegg lcl|cic:CICLE_v10014841mg 1 554 + 554 Gaps:30 100.00 538 79.18 0.0 hypothetical protein
blastp_kegg lcl|cit:102629224 1 554 + 554 Gaps:30 100.00 538 79.18 0.0 transcription initiation factor IIF subunit alpha-like
blastp_kegg lcl|vvi:100253654 6 554 + 549 Gaps:29 100.00 534 82.40 0.0 transcription initiation factor IIF subunit alpha-like
blastp_kegg lcl|pvu:PHAVU_003G083500g 1 554 + 554 Gaps:30 100.00 540 78.89 0.0 hypothetical protein
blastp_kegg lcl|pop:POPTR_0016s01680g 1 554 + 554 Gaps:32 100.00 540 80.00 0.0 POPTRDRAFT_254823 transcription initiation factor IIF alpha subunit family protein
blastp_kegg lcl|csv:101223580 1 553 + 553 Gaps:28 99.44 538 80.19 0.0 transcription initiation factor IIF subunit alpha-like
blastp_kegg lcl|csv:101207693 1 553 + 553 Gaps:28 99.44 538 80.19 0.0 transcription initiation factor IIF subunit alpha-like
blastp_kegg lcl|tcc:TCM_030350 1 554 + 554 Gaps:41 100.00 549 78.51 0.0 Transcription activators DNA binding RNA polymerase II transcription factors catalytics transcription initiation factors isoform 1
blastp_uniprot_sprot sp|Q9SU25|T2FA_ARATH 1 554 + 554 Gaps:37 93.37 649 69.14 0.0 Transcription initiation factor IIF subunit alpha OS Arabidopsis thaliana GN RAP74 PE 1 SV 1
blastp_uniprot_sprot sp|Q54WV4|T2FA_DICDI 132 214 + 83 Gaps:2 15.29 556 38.82 1e-09 General transcription factor IIF subunit 1 OS Dictyostelium discoideum GN gtf2f1 PE 3 SV 1
blastp_uniprot_sprot sp|O94416|T2FA_SCHPO 135 214 + 80 Gaps:4 14.07 540 40.79 6e-09 Transcription initiation factor IIF subunit alpha OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN tfg1 PE 1 SV 2
blastp_uniprot_sprot sp|P41895|T2FA_YEAST 135 218 + 84 Gaps:4 11.16 735 36.59 9e-07 Transcription initiation factor IIF subunit alpha OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN TFG1 PE 1 SV 2
rpsblast_cdd gnl|CDD|147767 72 553 + 482 Gaps:74 98.11 528 46.91 1e-108 pfam05793 TFIIF_alpha Transcription initiation factor IIF alpha subunit (TFIIF-alpha). Transcription initiation factor IIF alpha subunit (TFIIF-alpha) or RNA polymerase II-associating protein 74 (RAP74) is the large subunit of transcription factor IIF (TFIIF) which is essential for accurate initiation and stimulates elongation by RNA polymerase II.
rpsblast_cdd gnl|CDD|29316 21 206 + 186 Gaps:36 100.00 162 43.21 1e-33 cd00240 TFIIFa Transcription initiation factor IIF alpha subunit N-terminal region of RAP74. Subunit of transcription initiation complex involved in initiation elongation and promoter escape.Tetramer of 2 alpha and 2 beta TFIIF subunits interacts directly with RNA polymerase II. TFIIF inhibits non-specific transcription initiation by PolII and recruits the polymerase to the preinitiation complex on promoter DNA for site-specific transcription initiation. The PolII/TFIIF-complex attaches through direct interactions of TFIIF with promoter DNA TFIIB and the TAF250 subunit of TFIID and provides scaffolding for addition of TFIIE and TFIIH. Together with TFIIE TFIIF participates in DNA strand separation (open complex formation). N-terminal domains of RAP30 and RAP74 co-fold to form a single core structure a triple barrel heterodimer and has pseudo-2-fold symmetry..
rpsblast_cdd gnl|CDD|162113 192 413 + 222 Gaps:20 20.44 1096 23.21 4e-08 TIGR00927 2A1904 K+-dependent Na+/Ca+ exchanger.
rpsblast_cdd gnl|CDD|178775 249 498 + 250 Gaps:28 18.29 1465 18.66 6e-07 PLN03237 PLN03237 DNA topoisomerase 2 Provisional.
rpsblast_kog gnl|CDD|37604 1 554 + 554 Gaps:108 99.10 555 25.82 6e-60 KOG2393 KOG2393 KOG2393 Transcription initiation factor IIF large subunit (RAP74) [Transcription].
rpsblast_kog gnl|CDD|37043 241 379 + 139 Gaps:25 7.52 1516 28.95 6e-07 KOG1832 KOG1832 KOG1832 HIV-1 Vpr-binding protein [Cell cycle control cell division chromosome partitioning].

6 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
SUPERFAMILY 74 201 128 SSF50916 "Reactome:REACT_1675","Reactome:REACT_1788","Reactome:REACT_1892","Reactome:REACT_6185","Reactome:REACT_71","Reactome:REACT_769" none IPR011039
Gene3D 73 125 53 G3DSA:1.20.1130.10 "MetaCyc:PWY-101","MetaCyc:PWY-6785" none IPR001280
Pfam 72 552 481 PF05793 none Transcription initiation factor IIF, alpha subunit (TFIIF-alpha) IPR008851
PANTHER 67 554 488 PTHR13011 none none none
Gene3D 484 553 70 G3DSA:1.10.10.10 none none IPR011991
SUPERFAMILY 484 551 68 SSF46785 none none none

0 Localization

0 Qtllist

0 Targeting