Protein : Qrob_P0005110.2 Q. robur
Protein Identifier  ? Qrob_P0005110.2 Organism . Name  Quercus robur
Score  100.0 Score Type  egn
Protein Description  (M=2) PTHR12654//PTHR12654:SF0 - BILE ACID BETA-GLUCOSIDASE-RELATED // SUBFAMILY NOT NAMED Code Enzyme  EC:3.2.1.45
Gene Prediction Quality  validated Protein length 

Sequence

Length: 707  
Kegg Orthology  K17108
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0 Synonyms

4 GO Terms

Identifier Name Description
GO:0003824 catalytic activity Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic.
GO:0016021 integral component of membrane The component of a membrane consisting of the gene products and protein complexes having at least some part of their peptide sequence embedded in the hydrophobic region of the membrane.
GO:0004348 glucosylceramidase activity Catalysis of the reaction: D-glucosyl-N-acylsphingosine + H2O = D-glucose + N-acylsphingosine.
GO:0006665 sphingolipid metabolic process The chemical reactions and pathways involving sphingolipids, any of a class of lipids containing the long-chain amine diol sphingosine or a closely related base (a sphingoid).

20 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|pop:POPTR_0003s17650g 21 706 + 686 Gaps:6 71.22 966 79.65 0.0 POPTRDRAFT_711954 hypothetical protein
blastp_kegg lcl|vvi:100249401 17 706 + 690 Gaps:7 71.06 978 79.71 0.0 non-lysosomal glucosylceramidase-like
blastp_kegg lcl|pmum:103324329 17 706 + 690 Gaps:32 69.93 941 85.26 0.0 non-lysosomal glucosylceramidase
blastp_kegg lcl|pper:PRUPE_ppa001012mg 17 706 + 690 Gaps:32 70.45 934 85.11 0.0 hypothetical protein
blastp_kegg lcl|pvu:PHAVU_007G134300g 18 706 + 689 Gaps:23 69.95 955 80.69 0.0 hypothetical protein
blastp_kegg lcl|rcu:RCOM_1020360 17 706 + 690 Gaps:5 70.76 968 79.56 0.0 hypothetical protein
blastp_kegg lcl|cit:102614613 17 706 + 690 Gaps:17 70.40 956 81.58 0.0 non-lysosomal glucosylceramidase-like
blastp_kegg lcl|cic:CICLE_v10004255mg 17 706 + 690 Gaps:17 70.40 956 81.58 0.0 hypothetical protein
blastp_kegg lcl|gmx:100817476 18 706 + 689 Gaps:24 69.85 952 80.60 0.0 non-lysosomal glucosylceramidase-like
blastp_kegg lcl|gmx:100777599 18 706 + 689 Gaps:23 69.88 953 80.93 0.0 non-lysosomal glucosylceramidase-like
blastp_uniprot_sprot sp|Q69ZF3|GBA2_MOUSE 55 647 + 593 Gaps:12 55.01 918 50.10 5e-114 Non-lysosomal glucosylceramidase OS Mus musculus GN Gba2 PE 1 SV 2
blastp_uniprot_sprot sp|Q5M868|GBA2_RAT 25 647 + 623 Gaps:16 58.22 912 49.15 1e-111 Non-lysosomal glucosylceramidase OS Rattus norvegicus GN Gba2 PE 2 SV 2
blastp_uniprot_sprot sp|Q9HCG7|GBA2_HUMAN 55 647 + 593 Gaps:12 54.48 927 49.90 3e-111 Non-lysosomal glucosylceramidase OS Homo sapiens GN GBA2 PE 1 SV 2
blastp_uniprot_sprot sp|Q7KT91|C3390_DROME 25 658 + 634 Gaps:23 57.07 948 43.25 9e-57 Non-lysosomal glucosylceramidase OS Drosophila melanogaster GN CG33090 PE 1 SV 1
rpsblast_cdd gnl|CDD|191062 288 651 + 364 Gaps:15 100.00 357 51.26 1e-142 pfam04685 DUF608 Protein of unknown function DUF608. This family represents a conserved region with a pankaryotic distribution in a number of uncharacterized proteins.
rpsblast_cdd gnl|CDD|34072 74 653 + 580 Gaps:42 68.24 721 35.37 4e-71 COG4354 COG4354 Predicted bile acid beta-glucosidase [Carbohydrate transport and metabolism].
rpsblast_cdd gnl|CDD|152650 27 154 + 128 Gaps:12 38.59 311 31.67 8e-15 pfam12215 GBA2_N beta-Glucocerebrosidase 2 N terminal. This domain is found in bacteria archaea and eukaryotes. This domain is typically between 320 to 354 amino acids in length. This domain is found associated with pfam04685. This domain is found in the protein beta-Glucocerebrosidase 2. It is found just after the extreme N terminus. This protein is located in the ER. The N terminal is thought to be the luminal domain while the C terminal is the cytosolic domain. The catalytic domain of GBA-2 is unknown.
rpsblast_cdd gnl|CDD|33214 355 501 + 147 Gaps:19 23.40 641 30.67 2e-08 COG3408 GDB1 Glycogen debranching enzyme [Carbohydrate transport and metabolism].
rpsblast_cdd gnl|CDD|130624 355 570 + 216 Gaps:44 37.57 575 24.54 3e-07 TIGR01561 gde_arch glycogen debranching enzyme archaeal type putative. The seed for this model is composed of two uncharacterized archaeal proteins from Methanosarcina acetivorans and Sulfolobus solfataricus. Trusted cutoff is set so that essentially only archaeal members hit the model. The notable exceptions to archaeal membership are the Gram positive Clostridium perfringens which scores much better than some other archaea and the Cyanobacterium Nostoc sp. which scores just above the trusted cutoff. Noise cutoff is set to exclude the characterized eukaryotic glycogen debranching enzyme in S. cerevisiae. These cutoffs leave the prokaryotes Porphyromonas gingivalis and Deinococcus radiodurans below trusted but above noise. Multiple alignments including these last two species exhibit sequence divergence which may suggest a subtly different function for these prokaryotic proteins.
rpsblast_kog gnl|CDD|37330 18 677 + 660 Gaps:15 66.10 879 59.90 0.0 KOG2119 KOG2119 KOG2119 Predicted bile acid beta-glucosidase [Carbohydrate transport and metabolism].

9 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
PANTHER 16 195 180 PTHR12654 none none none
PANTHER 261 706 446 PTHR12654 none none none
SUPERFAMILY 300 647 348 SSF48208 none none IPR008928
ProSiteProfiles 1 33 33 PS51257 none Prokaryotic membrane lipoprotein lipid attachment site profile. none
Pfam 24 154 131 PF12215 "KEGG:00511+3.2.1.45","KEGG:00600+3.2.1.45" beta-Glucocerebrosidase 2 N terminal IPR024462
Gene3D 393 559 167 G3DSA:1.50.10.10 none none IPR012341
PANTHER 16 195 180 PTHR12654:SF0 none none none
PANTHER 261 706 446 PTHR12654:SF0 none none none
Pfam 288 650 363 PF04685 none Protein of unknown function, DUF608 IPR006775

0 Localization

17 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Champenoux_2015_nPriLBD_3P Qrob_Chr10 10 v_15000_157 v_15000_310 15,68 15,9 15,91 lod 2.4 5.5
Bourran2_2014_nSecLBD_3P Qrob_Chr08 8 s_1BN2OD_551 s_1B5AYF_599 17,17 0 43,51 lod 1,9229 4,4
Bourran2_2014_nLBD*_3P Qrob_Chr08 8 v_5216_549 v_11837_70 12,25 0 35,55 lod 2,5951 6
Bourran2_2014_nP*_3P Qrob_Chr08 8 v_5216_549 v_11837_70 12,19 0 31,97 lod 2,8472 6
Bourran2_2002_QTL11_peak_Bud_burst_A4 Qrob_Chr06 6 s_1C41PA_791 s_1AM1AV_1141 19,17 0 34,57 lod 2,3 2,9
Bourran2_2014_aSeqBC_3P Qrob_Chr06 6 v_506_189 v_686_77 30,72 13,58 43,48 lod 2,2746 6,3
Bourran2_2014_aSeqBC*_A4 Qrob_Chr06 6 s_2F5MK3_712 v_444_355 27,13 14,86 39,46 lod 3,7847 9,8
Bourran2_2014_nEpis*_3P Qrob_Chr08 8 s_1DA4QW_688 s_1DNI7D_820 17,96 0 37,75 lod 2,9745 7,5
Bourran2_2014_nEpis*_A4 Qrob_Chr07 7 v_12400_446 s_1BPEBU_1211 6,93 0 15,13 lod 4,7411 11
Bourran2_2014_nFork*_3P Qrob_Chr06 6 v_12930_125 s_1AMZEI_909 34,28 7,43 41,48 lod 2,4044 5,5
Bourran2_2014_nLBD_A4 Qrob_Chr06 6 v_12930_125 s_1AMZEI_909 37,41 9,8 50,1 lod 1,9524 4,1
Bourran2_2014_nPriBD_A4 Qrob_Chr06 6 v_12930_125 s_1AMZEI_909 34,51 3,36 51,86 lod 1,6747 3,9
Bourran2_2014_nPriLBD*_3P Qrob_Chr11 11 v_12066_307 s_1B15GJ_447 3,37 0,57 16,17 lod 3,4299 6,9
Bourran2_2014_nSecLBD_A4 Qrob_Chr07 7 v_8327_222 s_1A4WGY_363 16,04 0 44,69 lod 2,6373 6,5
Bourran2_2014_vEpiBC_A4 Qrob_Chr06 6 v_12930_125 s_1AMZEI_909 37,55 14,41 50,01 lod 1,7882 4,8
Champenoux_2015_nEpis_3P Qrob_Chr11 11 s_1DG9PM_867 s_1BZ083_1312 26,53 25,47 27,72 lod 4.4 8.9
Champenoux_2015_nP_3P Qrob_Chr06 6 s_1A386O_228 s_1AYZFS_603 27,03 26,47 27,34 lod 2.8 7.2

1 Targeting

Analysis Start End Length Location Reliability Signal Peptide Cut Off Mitochondrion Cut Off Network Signal Peptide Length
TargetP 1 94   Mitochondrion 4 0.048 0.594 NON-PLANT 94