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Genome sequences and databases

Botrytis cinerea genome from isolate T4 was sequenced and assembled by Genoscope, France.
 First cDNA library was constructed from poly(A)+ mRNA isolated from B. cinerea (T4) mycelium grown under axenic culture conditions and sequenced by genoscope.

  • Download Botrytis genome sequences (supercontigs, contigs)
    • Available blasts are: blastn, tblastn,blastp
    • Available databases are: Botrytis : Genome contigs, ORFs (DNA and Proteins)...
  • Quick Search
    GnpIs give access to several databases of organisms and biological information using a simple but powerfull form.
     Since release 1.5.1, Botrytis database has been indexed and pertinent matches found are directly linked to the corresponding GnpGenome track.
     Special terms are authorized and results obtain with "membrane" are not equal to "*membrane*".
     For more information on queries, see the associated documentation.
  • Advanced Search
    BioMart is another way to explore Botrytis genome, building queries on features and specific types of data.
     Results are supplied as a table, very convenient for extracting information and performing post-treatment, helping biologists to get list of interests.
  • GnpGenome Botrytis : Genome Browser for Botrytis T4 Structural annotation : A database based on the chado model (GMOD) gathers all the information produced the structural annotation pipeline of Botrytis cinerea T4 isolate genome.
    • prediction pipeline has been processed through ab initio gene finding softwares and similarity methods. All results have been then gathered and processed by Eugene to predict gene models. 14250 gene models have been predicted (94% have at least an evidence: expression, domain/motif, Bi-Directional Best Hit with a related genome Sclerotinia sclerotiorum), other 2110 genes are small (length < 100aa) without evidence
    • Uniprot and a Fungal protein database (containing proteins from 30 fungal genomes)
    • 83000 ESTs (9667 unisequences) from 14 B. cinerea cDNA libraries
    • 70000 ESTs from S. sclerotiorum ( Broad Institute )
    • Genes from B. cinerea (B05.10 isolate) and S. sclerotiorum (Broad Institute)
    • Genomes of B. cinerea (B05.10 isolate) and S. sclerotiorum (Broad Institute)
    • Repeats from RepBase
    • Tandem repeats found by TRF
  • GnpArray :
     A Nimblegen 1-plex array was designed using 21,200 B. cinerea gene models corresponding to 19,454 CDS either identified in T4 or B05.10 genomes, 12 experimental genes and 1,734 additional EST unisequences.
     A second Nimblegen 1-plex array was designed using 15,026 S. sclerotiorum gene models corresponding to 14,522 ORFs and 504 additional EST unisequences
    Expression of fungal genes was studied during infection on sunflower cotyledon, and compared with in vitro expression. The experimental conditions were: (i) Infection of sunflower cotyledons by mycelial plugs of B. cinerea (B05.10) and S. sclerotiorum at 2 days after inoculation (100% of the surface area was infected), (ii) mycelial cultures grown in vitro (malt agar) for each fungal strain, and (iii) non-infected sunflower cotyledons.
Update: 21 Jan 2016
Creation date: 06 Apr 2010