Protein : Qrob_P0759600.2 Q. robur
Protein Identifier  ? Qrob_P0759600.2 Organism . Name  Quercus robur
Score  13.2 Score Type  egn
Protein Description  (M=1) 1.4.1.14 - Glutamate synthase (NADH). Code Enzyme  EC:1.4.1.14
Gene Prediction Quality  validated Protein length 

Sequence

Length: 2257  
Kegg Orthology  K00264
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0 Synonyms

15 GO Terms

Identifier Name Description
GO:0008152 metabolic process The chemical reactions and pathways, including anabolism and catabolism, by which living organisms transform chemical substances. Metabolic processes typically transform small molecules, but also include macromolecular processes such as DNA repair and replication, and protein synthesis and degradation.
GO:0016491 oxidoreductase activity Catalysis of an oxidation-reduction (redox) reaction, a reversible chemical reaction in which the oxidation state of an atom or atoms within a molecule is altered. One substrate acts as a hydrogen or electron donor and becomes oxidized, while the other acts as hydrogen or electron acceptor and becomes reduced.
GO:0005506 iron ion binding Interacting selectively and non-covalently with iron (Fe) ions.
GO:0055114 oxidation-reduction process A metabolic process that results in the removal or addition of one or more electrons to or from a substance, with or without the concomitant removal or addition of a proton or protons.
GO:0003824 catalytic activity Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic.
GO:0050660 flavin adenine dinucleotide binding Interacting selectively and non-covalently with FAD, flavin-adenine dinucleotide, the coenzyme or the prosthetic group of various flavoprotein oxidoreductase enzymes, in either the oxidized form, FAD, or the reduced form, FADH2.
GO:0051536 iron-sulfur cluster binding Interacting selectively and non-covalently with an iron-sulfur cluster, a combination of iron and sulfur atoms.
GO:0010181 FMN binding Interacting selectively and non-covalently with FMN, flavin mononucleotide, the coenzyme or the prosthetic group of various flavoprotein oxidoreductase enzymes.
GO:0006807 nitrogen compound metabolic process The chemical reactions and pathways involving organic or inorganic compounds that contain nitrogen, including (but not limited to) nitrogen fixation, nitrification, denitrification, assimilatory/dissimilatory nitrate reduction and the interconversion of nitrogenous organic matter and ammonium.
GO:0006537 glutamate biosynthetic process The chemical reactions and pathways resulting in the formation of glutamate, the anion of 2-aminopentanedioic acid.
GO:0015930 glutamate synthase activity Catalysis of the formation of L-glutamine and 2-oxoglutarate from L-glutamate, using NADH, NADPH or ferredoxin as hydrogen acceptors.
GO:0016638 oxidoreductase activity, acting on the CH-NH2 group of donors Catalysis of an oxidation-reduction (redox) reaction in which a CH-NH2 group acts as a hydrogen or electron donor and reduces a hydrogen or electron acceptor.
GO:0016639 oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor Catalysis of an oxidation-reduction (redox) reaction in which a CH-NH2 group acts as a hydrogen or electron donor and reduces NAD+ or NADP.
GO:0016040 glutamate synthase (NADH) activity Catalysis of the reaction: 2 L-glutamate + NAD(+) = 2-oxoglutarate + L-glutamine + H(+) + NADH.
GO:0045181 glutamate synthase activity, NAD(P)H as acceptor Catalysis of the reaction: 2 L-glutamate + NAD(P)+ = L-glutamine + 2-oxoglutarate + NAD(P)H + H+.

44 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|vvi:100246868 3 2256 + 2254 Gaps:48 99.82 2216 88.07 0.0 glutamate synthase 1 [NADH] chloroplastic-like
blastp_kegg lcl|rcu:RCOM_1579610 19 2256 + 2238 Gaps:48 99.23 2215 88.35 0.0 glutamate synthase putative (EC:1.4.1.14)
blastp_kegg lcl|pper:PRUPE_ppa000037mg 1 2256 + 2256 Gaps:53 100.00 2207 88.36 0.0 hypothetical protein
blastp_kegg lcl|pmum:103333633 1 2256 + 2256 Gaps:67 100.00 2193 88.10 0.0 glutamate synthase [NADH] amyloplastic
blastp_kegg lcl|mdm:103443818 1 2256 + 2256 Gaps:54 100.00 2208 87.27 0.0 glutamate synthase [NADH] amyloplastic
blastp_kegg lcl|pop:POPTR_0015s01950g 34 2256 + 2223 Gaps:46 98.38 2221 87.55 0.0 POPTRDRAFT_824538 NADH-dependent glutamate synthase family protein
blastp_kegg lcl|cit:102620225 4 2256 + 2253 Gaps:42 99.82 2217 86.40 0.0 glutamate synthase 1 [NADH] chloroplastic-like
blastp_kegg lcl|cmo:103485701 1 2256 + 2256 Gaps:46 100.00 2218 85.93 0.0 glutamate synthase [NADH] amyloplastic
blastp_kegg lcl|csv:101213224 1 2256 + 2256 Gaps:42 100.00 2222 85.69 0.0 glutamate synthase [NADH] amyloplastic-like
blastp_kegg lcl|csv:101225700 1 2256 + 2256 Gaps:42 100.00 2222 85.64 0.0 glutamate synthase [NADH] amyloplastic-like
blastp_pdb 2vdc_F 120 1661 + 1542 Gaps:93 99.25 1472 45.72 0.0 mol:protein length:1472 GLUTAMATE SYNTHASE [NADPH] LARGE CHAIN
blastp_pdb 2vdc_E 120 1661 + 1542 Gaps:93 99.25 1472 45.72 0.0 mol:protein length:1472 GLUTAMATE SYNTHASE [NADPH] LARGE CHAIN
blastp_pdb 2vdc_D 120 1661 + 1542 Gaps:93 99.25 1472 45.72 0.0 mol:protein length:1472 GLUTAMATE SYNTHASE [NADPH] LARGE CHAIN
blastp_pdb 2vdc_C 120 1661 + 1542 Gaps:93 99.25 1472 45.72 0.0 mol:protein length:1472 GLUTAMATE SYNTHASE [NADPH] LARGE CHAIN
blastp_pdb 2vdc_B 120 1661 + 1542 Gaps:93 99.25 1472 45.72 0.0 mol:protein length:1472 GLUTAMATE SYNTHASE [NADPH] LARGE CHAIN
blastp_pdb 2vdc_A 120 1661 + 1542 Gaps:93 99.25 1472 45.72 0.0 mol:protein length:1472 GLUTAMATE SYNTHASE [NADPH] LARGE CHAIN
blastp_pdb 1ea0_B 120 1661 + 1542 Gaps:93 98.78 1479 45.72 0.0 mol:protein length:1479 GLUTAMATE SYNTHASE [NADPH] LARGE CHAIN
blastp_pdb 1ea0_A 120 1661 + 1542 Gaps:93 98.78 1479 45.72 0.0 mol:protein length:1479 GLUTAMATE SYNTHASE [NADPH] LARGE CHAIN
blastp_pdb 1ofe_B 120 1659 + 1540 Gaps:120 98.42 1520 45.32 0.0 mol:protein length:1520 FERREDOXIN-DEPENDENT GLUTAMATE SYNTHASE 2
blastp_pdb 1ofe_A 120 1659 + 1540 Gaps:120 98.42 1520 45.32 0.0 mol:protein length:1520 FERREDOXIN-DEPENDENT GLUTAMATE SYNTHASE 2
blastp_uniprot_sprot sp|Q03460|GLSN_MEDSA 15 2256 + 2242 Gaps:53 99.95 2194 85.23 0.0 Glutamate synthase [NADH] amyloplastic OS Medicago sativa PE 1 SV 1
blastp_uniprot_sprot sp|Q9LV03|GLUT1_ARATH 3 2241 + 2239 Gaps:38 99.68 2208 84.23 0.0 Glutamate synthase 1 [NADH] chloroplastic OS Arabidopsis thaliana GN GLT1 PE 1 SV 2
blastp_uniprot_sprot sp|Q0JKD0|GLT1_ORYSJ 100 2224 + 2125 Gaps:63 95.52 2167 81.69 0.0 Glutamate synthase 1 [NADH] chloroplastic OS Oryza sativa subsp. japonica GN Os01g0681900 PE 2 SV 1
blastp_uniprot_sprot sp|Q0DG35|GLT2_ORYSJ 99 2223 + 2125 Gaps:52 95.11 2188 79.34 0.0 Glutamate synthase 2 [NADH] chloroplastic OS Oryza sativa subsp. japonica GN Os05g0555600 PE 2 SV 2
blastp_uniprot_sprot sp|Q9C102|GLT1_SCHPO 106 2215 + 2110 Gaps:107 96.40 2111 57.40 0.0 Putative glutamate synthase [NADPH] OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN glt1 PE 2 SV 1
blastp_uniprot_sprot sp|Q12680|GLT1_YEAST 97 2218 + 2122 Gaps:114 97.25 2145 52.16 0.0 Glutamate synthase [NADH] OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN GLT1 PE 1 SV 2
blastp_uniprot_sprot sp|P55037|GLTB_SYNY3 109 1670 + 1562 Gaps:81 96.06 1550 54.73 0.0 Ferredoxin-dependent glutamate synthase 1 OS Synechocystis sp. (strain PCC 6803 / Kazusa) GN gltB PE 1 SV 1
blastp_uniprot_sprot sp|P96218|GLTB_MYCTU 106 1682 + 1577 Gaps:86 99.35 1527 52.54 0.0 Glutamate synthase [NADPH] large chain OS Mycobacterium tuberculosis GN gltB PE 1 SV 3
blastp_uniprot_sprot sp|P39812|GLTA_BACSU 106 1682 + 1577 Gaps:106 97.83 1520 51.04 0.0 Glutamate synthase [NADPH] large chain OS Bacillus subtilis (strain 168) GN gltA PE 2 SV 3
blastp_uniprot_sprot sp|Q05755|GLTB_AZOBR 111 1661 + 1551 Gaps:93 97.03 1515 45.65 0.0 Glutamate synthase [NADPH] large chain OS Azospirillum brasilense GN gltB PE 1 SV 1

31 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
Pfam 120 496 377 PF00310 none Glutamine amidotransferases class-II IPR000583
SUPERFAMILY 1403 1667 265 SSF69336 none none IPR002489
Gene3D 2143 2224 82 G3DSA:3.50.50.60 none none none
Gene3D 1019 1391 373 G3DSA:3.20.20.70 none none IPR013785
SUPERFAMILY 120 550 431 SSF56235 none none IPR029055
TIGRFAM 1736 2220 485 TIGR01317 none GOGAT_sm_gam: glutamate synthase, NADH/NADPH, small subunit IPR006005
PRINTS 2026 2040 15 PR00419 none Adrenodoxin reductase family signature none
PRINTS 1948 1958 11 PR00419 none Adrenodoxin reductase family signature none
PRINTS 1882 1904 23 PR00419 none Adrenodoxin reductase family signature none
PRINTS 1905 1918 14 PR00419 none Adrenodoxin reductase family signature none
Pfam 1432 1624 193 PF01493 none GXGXG motif IPR002489
SUPERFAMILY 1734 1891 158 SSF46548 none none IPR009051
Pfam 630 915 286 PF04898 "UniPathway:UPA00045" Glutamate synthase central domain IPR006982
Gene3D 1882 1983 102 G3DSA:3.50.50.60 none none none
Pfam 980 1350 371 PF01645 none Conserved region in glutamate synthase IPR002932
PANTHER 619 1672 1054 PTHR11938:SF1 none none none
PANTHER 102 570 469 PTHR11938:SF1 none none none
PANTHER 619 1672 1054 PTHR11938 none none none
PANTHER 102 570 469 PTHR11938 none none none
Pfam 1759 1869 111 PF14691 none Dihydroprymidine dehydrogenase domain II, 4Fe-4S cluster IPR028261
Gene3D 1736 1881 146 G3DSA:1.10.1060.10 none none none
PIRSF 34 2248 2215 PIRSF000187 "KEGG:00250+1.4.1.14","KEGG:00910+1.4.1.14","MetaCyc:PWY-6963","UniPathway:UPA00045","UniPathway:UPA00634" none IPR012220
Pfam 1882 2196 315 PF07992 none Pyridine nucleotide-disulphide oxidoreductase IPR023753
Gene3D 1984 2142 159 G3DSA:3.50.50.60 none none none
Gene3D 628 953 326 G3DSA:3.20.20.70 none none IPR013785
Gene3D 1409 1680 272 G3DSA:2.160.20.60 none none IPR002489
ProSiteProfiles 120 524 405 PS51278 none Glutamine amidotransferase type 2 domain profile. IPR017932
Coils 1669 1690 22 Coil none none none
SUPERFAMILY 614 1386 773 SSF51395 none none none
SUPERFAMILY 1878 2218 341 SSF51905 none none none

0 Localization

0 Qtllist

0 Targeting