Protein : Qrob_P0637150.2 Q. robur
Protein Identifier  ? Qrob_P0637150.2 Organism . Name  Quercus robur
Score  98.0 Score Type  egn
Protein Description  (M=2) 2.8.1.8 - Lipoyl synthase. Code Enzyme  EC:2.8.1.8
Gene Prediction Quality  validated Protein length 

Sequence

Length: 367  
Kegg Orthology  K03644
Paste the following link
Lists
This Protein isn't in any lists. Upload a list.

Sequence Feature Displayer

Protein Sequence Displayer

J Browse Displayer

0 Synonyms

5 GO Terms

Identifier Name Description
GO:0003824 catalytic activity Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic.
GO:0051539 4 iron, 4 sulfur cluster binding Interacting selectively and non-covalently with a 4 iron, 4 sulfur (4Fe-4S) cluster; this cluster consists of four iron atoms, with the inorganic sulfur atoms found between the irons and acting as bridging ligands.
GO:0051536 iron-sulfur cluster binding Interacting selectively and non-covalently with an iron-sulfur cluster, a combination of iron and sulfur atoms.
GO:0009107 lipoate biosynthetic process The chemical reactions and pathways resulting in the formation of lipoate, 1,2-dithiolane-3-pentanoate, the anion derived from lipoic acid.
GO:0016992 lipoate synthase activity Catalysis of the reaction: protein N6-(octanoyl)lysine + 2 sulfur + 2 S-adenosyl-L-methionine = protein N6-(lipoyl)lysine + 2 L-methionine + 2 5'-deoxyadenosyl.

31 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|pvu:PHAVU_005G072800g 1 355 + 355 Gaps:14 96.29 377 81.27 0.0 hypothetical protein
blastp_kegg lcl|gmx:100789911 1 355 + 355 Gaps:15 96.30 378 81.59 0.0 lipoyl synthase 2 mitochondrial-like
blastp_kegg lcl|mdm:103419811 1 355 + 355 Gaps:16 96.05 380 79.18 0.0 lipoyl synthase 2 mitochondrial-like
blastp_kegg lcl|pop:POPTR_0007s06900g 1 336 + 336 Gaps:18 100.00 354 79.66 0.0 hypothetical protein
blastp_kegg lcl|gmx:100775835 1 355 + 355 Gaps:15 95.29 382 81.59 0.0 lipoyl synthase 2 mitochondrial-like
blastp_kegg lcl|pvu:PHAVU_005G106700g 1 355 + 355 Gaps:14 95.28 381 80.72 0.0 hypothetical protein
blastp_kegg lcl|sot:102581126 16 355 + 340 Gaps:10 90.53 380 83.72 0.0 lipoyl synthase mitochondrial-like
blastp_kegg lcl|pper:PRUPE_ppa007143mg 29 355 + 327 Gaps:8 86.58 380 83.89 0.0 hypothetical protein
blastp_kegg lcl|sly:101258875 16 355 + 340 Gaps:10 90.53 380 83.43 0.0 lipoyl synthase mitochondrial-like
blastp_kegg lcl|mdm:103404733 1 355 + 355 Gaps:17 96.06 381 78.69 0.0 lipoyl synthase mitochondrial
blastp_uniprot_sprot sp|B7FM45|LIAS_MEDTR 27 355 + 329 Gaps:10 88.10 378 82.58 0.0 Lipoyl synthase mitochondrial OS Medicago truncatula GN LIP1 PE 2 SV 1
blastp_uniprot_sprot sp|B9H5L9|LIAS_POPTR 1 355 + 355 Gaps:21 96.10 385 79.73 0.0 Lipoyl synthase mitochondrial OS Populus trichocarpa GN LIP1 PE 3 SV 1
blastp_uniprot_sprot sp|Q3LSN5|LIAS2_PEA 26 355 + 330 Gaps:10 88.83 376 83.53 0.0 Lipoyl synthase 2 mitochondrial OS Pisum sativum GN LIP1-2 PE 2 SV 1
blastp_uniprot_sprot sp|A5CB81|LIAS_VITVI 28 355 + 328 Gaps:10 84.48 393 81.93 0.0 Lipoyl synthase mitochondrial OS Vitis vinifera GN LIP1 PE 3 SV 1
blastp_uniprot_sprot sp|Q3LSN4|LIAS1_PEA 26 355 + 330 Gaps:10 88.83 376 83.53 0.0 Lipoyl synthase 1 mitochondrial OS Pisum sativum GN LIP1-1 PE 2 SV 1
blastp_uniprot_sprot sp|B9RW49|LIAS_RICCO 1 323 + 323 Gaps:13 95.40 348 82.23 0.0 Lipoyl synthase mitochondrial OS Ricinus communis GN LIP1 PE 3 SV 1
blastp_uniprot_sprot sp|A2XU53|LIAS_ORYSI 33 353 + 321 Gaps:3 83.25 382 80.50 0.0 Lipoyl synthase mitochondrial OS Oryza sativa subsp. indica GN LIP1 PE 3 SV 2
blastp_uniprot_sprot sp|C5Y9R0|LIAS_SORBI 30 353 + 324 Gaps:3 83.16 386 80.06 0.0 Lipoyl synthase mitochondrial OS Sorghum bicolor GN LIP1 PE 3 SV 1
blastp_uniprot_sprot sp|Q7XRF1|LIAS_ORYSJ 33 353 + 321 Gaps:3 83.25 382 80.19 0.0 Lipoyl synthase mitochondrial OS Oryza sativa subsp. japonica GN LIP1 PE 2 SV 2
blastp_uniprot_sprot sp|B8A031|LIAS_MAIZE 1 353 + 353 Gaps:3 91.38 383 74.29 0.0 Lipoyl synthase mitochondrial OS Zea mays GN LIP1 PE 2 SV 1
rpsblast_cdd gnl|CDD|178048 19 328 + 310 Gaps:5 89.68 349 71.25 1e-171 PLN02428 PLN02428 lipoic acid synthase.
rpsblast_cdd gnl|CDD|180115 59 328 + 270 Gaps:8 90.66 289 54.58 1e-116 PRK05481 PRK05481 lipoyl synthase Provisional.
rpsblast_cdd gnl|CDD|173603 57 328 + 272 Gaps:5 69.60 398 53.07 1e-110 PTZ00413 PTZ00413 lipoate synthase Provisional.
rpsblast_cdd gnl|CDD|30668 58 328 + 271 Gaps:8 85.95 306 53.61 1e-102 COG0320 LipA Lipoate synthase [Coenzyme metabolism].
rpsblast_cdd gnl|CDD|161907 59 344 + 286 Gaps:10 91.39 302 49.28 6e-96 TIGR00510 lipA lipoate synthase. This enzyme is an iron-sulfur protein. It is localized to mitochondria in yeast and Arabidopsis. It generates lipoic acid a thiol antioxidant that is linked to a specific Lys as prosthetic group for the pyruvate and alpha-ketoglutarate dehydrogenase complexes and the glycine-cleavage system. The family shows strong sequence conservation.
rpsblast_cdd gnl|CDD|183830 64 328 + 265 Gaps:9 88.97 290 46.90 7e-96 PRK12928 PRK12928 lipoyl synthase Provisional.
rpsblast_cdd gnl|CDD|202871 120 286 + 167 Gaps:8 100.00 165 18.18 1e-16 pfam04055 Radical_SAM Radical SAM superfamily. Radical SAM proteins catalyze diverse reactions including unusual methylations isomerisation sulphur insertion ring formation anaerobic oxidation and protein radical formation.
rpsblast_cdd gnl|CDD|197846 116 300 + 185 Gaps:11 87.96 216 15.79 3e-15 smart00729 Elp3 Elongator protein 3 MiaB family Radical SAM. This superfamily contains MoaA NifB PqqE coproporphyrinogen III oxidase biotin synthase and MiaB families and includes a representative in the eukaryotic elongator subunit Elp-3. Some members of the family are methyltransferases.
rpsblast_cdd gnl|CDD|31260 126 288 + 163 Gaps:20 46.22 370 22.81 4e-08 COG1060 ThiH Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes [Coenzyme metabolism / General function prediction only].
rpsblast_cdd gnl|CDD|100105 120 300 + 181 Gaps:8 87.75 204 16.76 1e-07 cd01335 Radical_SAM Radical SAM superfamily. Enzymes of this family generate radicals by combining a 4Fe-4S cluster and S-adenosylmethionine (SAM) in close proximity. They are characterized by a conserved CxxxCxxC motif which coordinates the conserved iron-sulfur cluster. Mechanistically they share the transfer of a single electron from the iron-sulfur cluster to SAM which leads to its reductive cleavage to methionine and a 5'-deoxyadenosyl radical which in turn abstracts a hydrogen from the appropriately positioned carbon atom. Depending on the enzyme SAM is consumed during this process or it is restored and reused. Radical SAM enzymes catalyze steps in metabolism DNA repair the biosynthesis of vitamins and coenzymes and the biosynthesis of many antibiotics. Examples are biotin synthase (BioB) lipoyl synthase (LipA) pyruvate formate-lyase (PFL) coproporphyrinogen oxidase (HemN) lysine 2 3-aminomutase (LAM) anaerobic ribonucleotide reductase (ARR) and MoaA an enzyme of the biosynthesis of molybdopterin..

11 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
PANTHER 47 328 282 PTHR10949 "KEGG:00785+2.8.1.8","MetaCyc:PWY-6987","MetaCyc:PWY-7381","MetaCyc:PWY-7382","UniPathway:UPA00538";signature_desc=LIPOYL SYNTHASE none IPR003698
Phobius 355 366 12 CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. none
Phobius 335 354 20 TRANSMEMBRANE none Region of a membrane-bound protein predicted to be embedded in the membrane. none
Pfam 123 285 163 PF04055 none Radical SAM superfamily IPR007197
PANTHER 47 328 282 PTHR10949:SF9 none none none
Gene3D 81 318 238 G3DSA:3.20.20.70 none none IPR013785
SUPERFAMILY 81 328 248 SSF102114 none none none
Phobius 1 334 334 NON_CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. none
TIGRFAM 59 344 286 TIGR00510 "KEGG:00785+2.8.1.8","MetaCyc:PWY-6987","MetaCyc:PWY-7381","MetaCyc:PWY-7382","UniPathway:UPA00538" lipA: lipoyl synthase IPR003698
Hamap 65 363 299 MF_00206 "KEGG:00785+2.8.1.8","MetaCyc:PWY-6987","MetaCyc:PWY-7381","MetaCyc:PWY-7382","UniPathway:UPA00538" Lipoyl synthase [lipA]. IPR003698
SMART 116 323 208 SM00729 none Elongator protein 3, MiaB family, Radical SAM IPR006638

2 Localization

Analysis Start End Length
SignalP_GRAM_POSITIVE 1 24 23
TMHMM 333 355 22

0 Qtllist

0 Targeting