Protein : Qrob_P0551070.2 Q. robur
Protein Identifier  ? Qrob_P0551070.2 Organism . Name  Quercus robur
Score  94.0 Score Type  egn
Protein Description  (M=7) 3.2.1.24 - Alpha-mannosidase. Code Enzyme  EC:3.2.1.24
Gene Prediction Quality  validated Protein length 

Sequence

Length: 327  
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0 Synonyms

3 GO Terms

Identifier Name Description
GO:0003824 catalytic activity Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic.
GO:0005975 carbohydrate metabolic process The chemical reactions and pathways involving carbohydrates, any of a group of organic compounds based of the general formula Cx(H2O)y. Includes the formation of carbohydrate derivatives by the addition of a carbohydrate residue to another molecule.
GO:0004559 alpha-mannosidase activity Catalysis of the hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.

21 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|pper:PRUPE_ppa000717mg 1 66 + 66 Gaps:2 6.64 1024 75.00 6e-24 hypothetical protein
blastp_kegg lcl|tcc:TCM_046752 1 66 + 66 none 6.48 1018 77.27 7e-24 Glycosyl hydrolase family 38 protein
blastp_kegg lcl|pmum:103330438 1 66 + 66 Gaps:2 6.64 1024 73.53 8e-24 lysosomal alpha-mannosidase
blastp_kegg lcl|aly:ARALYDRAFT_488225 4 66 + 63 none 6.15 1024 74.60 3e-23 glycosyl hydrolase family 38 protein
blastp_kegg lcl|pvu:PHAVU_001G084700g 17 66 + 50 none 4.86 1029 96.00 4e-23 hypothetical protein
blastp_kegg lcl|gmx:100776282 17 66 + 50 none 4.85 1030 96.00 4e-23 lysosomal alpha-mannosidase-like
blastp_kegg lcl|cit:102627684 4 66 + 63 none 6.24 1010 74.60 4e-23 lysosomal alpha-mannosidase-like
blastp_kegg lcl|cmo:103503143 4 66 + 63 none 6.18 1020 74.60 8e-23 lysosomal alpha-mannosidase
blastp_kegg lcl|vvi:100244533 6 66 + 61 none 5.95 1025 78.69 9e-23 lysosomal alpha-mannosidase-like
blastp_kegg lcl|cit:102622809 1 66 + 66 Gaps:3 6.73 1026 73.91 1e-22 lysosomal alpha-mannosidase-like
blastp_pdb 1o7d_A 25 86 + 62 Gaps:12 24.83 298 48.65 4e-11 mol:protein length:298 LYSOSOMAL ALPHA-MANNOSIDASE
blastp_uniprot_sprot sp|Q8VHC8|MA2B1_CAVPO 25 66 + 42 none 4.17 1007 71.43 9e-12 Lysosomal alpha-mannosidase OS Cavia porcellus GN MAN2B1 PE 1 SV 1
blastp_uniprot_sprot sp|Q29451|MA2B1_BOVIN 25 86 + 62 Gaps:12 7.41 999 48.65 3e-11 Lysosomal alpha-mannosidase OS Bos taurus GN MAN2B1 PE 1 SV 4
blastp_uniprot_sprot sp|O46432|MA2B1_FELCA 25 66 + 42 none 4.17 1007 66.67 2e-10 Lysosomal alpha-mannosidase OS Felis catus GN MAN2B1 PE 2 SV 1
blastp_uniprot_sprot sp|Q60HE9|MA2B1_MACFA 25 66 + 42 none 4.15 1012 66.67 3e-10 Lysosomal alpha-mannosidase OS Macaca fascicularis GN MAN2B1 PE 2 SV 1
blastp_uniprot_sprot sp|O09159|MA2B1_MOUSE 3 76 + 74 Gaps:9 7.40 1013 49.33 5e-10 Lysosomal alpha-mannosidase OS Mus musculus GN Man2b1 PE 2 SV 4
blastp_uniprot_sprot sp|O00754|MA2B1_HUMAN 25 66 + 42 none 4.15 1011 64.29 5e-10 Lysosomal alpha-mannosidase OS Homo sapiens GN MAN2B1 PE 1 SV 3
blastp_uniprot_sprot sp|P34098|MANA_DICDI 36 65 + 30 none 2.97 1010 86.67 2e-09 Lysosomal alpha-mannosidase OS Dictyostelium discoideum GN manA PE 1 SV 2
rpsblast_cdd gnl|CDD|212121 36 67 + 32 none 11.51 278 90.62 2e-16 cd10810 GH38N_AMII_LAM_like N-terminal catalytic domain of lysosomal alpha-mannosidase and similar proteins glycoside hydrolase family 38 (GH38). The subfamily is represented by lysosomal alpha-mannosidase (LAM Man2B1 EC 3.2.1.114) which is a broad specificity exoglycosidase hydrolyzing all known alpha 1 2- alpha 1 3- and alpha 1 6-mannosidic linkages from numerous high mannose type oligosaccharides. LAM is expressed in all tissues and in many species. In mammals the absence of LAM can cause the autosomal recessive disease alpha-mannosidosis. LAM has an acidic pH optimum at 4.0-4.5. It is stimulated by zinc ion and is inhibited by cobalt ion and plant alkaloids such as swainsonine (SW). LAM catalyzes hydrolysis by a double displacement mechanism in which a glycosyl-enzyme intermediate is formed and hydrolyzed via oxacarbenium ion-like transition states. A carboxylic acid in the active site acts as the catalytic nucleophile in the formation of the covalent intermediate while a second carboxylic acid acts as a general acid catalyst. The same residue is thought to assist in the hydrolysis (deglycosylation) step this time acting as a general base.
rpsblast_cdd gnl|CDD|212095 36 65 + 30 none 11.63 258 66.67 4e-09 cd00451 GH38N_AMII_euk N-terminal catalytic domain of eukaryotic class II alpha-mannosidases glycoside hydrolase family 38 (GH38). The family corresponds to a group of eukaryotic class II alpha-mannosidases (AlphaMII) which contain Golgi alpha-mannosidases II (GMII) the major broad specificity lysosomal alpha-mannosidases (LAM MAN2B1) the noval core-specific lysosomal alpha 1 6-mannosidases (Epman MAN2B2) and similar proteins. GMII catalyzes the hydrolysis of the terminal both alpha-1 3-linked and alpha-1 6-linked mannoses from the high-mannose oligosaccharide GlcNAc(Man)5(GlcNAc)2 to yield GlcNAc(Man)3(GlcNAc)2 (GlcNAc N-acetylglucosmine) which is the committed step of complex N-glycan synthesis. LAM is a broad specificity exoglycosidase hydrolyzing all known alpha 1 2- alpha 1 3- and alpha 1 6-mannosidic linkages from numerous high mannose type oligosaccharides. Different from LAM Epman can efficiently cleave only the alpha 1 6-linked mannose residue from (Man)3GlcNAc but not (Man)3(GlcNAc)2 or other larger high mannose oligosaccharides in the core of N-linked glycans. Members in this family are retaining glycosyl hydrolases of family GH38 that employs a two-step mechanism involving the formation of a covalent glycosyl enzyme complex. Two carboxylic acids positioned within the active site act in concert: one as a catalytic nucleophile and the other as a general acid/base catalyst.
rpsblast_kog gnl|CDD|37170 4 67 + 64 none 6.43 996 56.25 4e-19 KOG1959 KOG1959 KOG1959 Glycosyl hydrolase family 38 - alpha-mannosidase [Carbohydrate transport and metabolism].

16 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
Phobius 134 154 21 TRANSMEMBRANE none Region of a membrane-bound protein predicted to be embedded in the membrane. none
Phobius 18 66 49 NON_CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. none
Gene3D 36 71 36 G3DSA:3.20.110.10 none none IPR027291
Phobius 13 17 5 SIGNAL_PEPTIDE_C_REGION none C-terminal region of a signal peptide. none
Phobius 67 84 18 TRANSMEMBRANE none Region of a membrane-bound protein predicted to be embedded in the membrane. none
Phobius 85 103 19 CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. none
Phobius 1 17 17 SIGNAL_PEPTIDE none Signal peptide region none
Pfam 37 71 35 PF01074 none Glycosyl hydrolases family 38 N-terminal domain IPR000602
Phobius 155 326 172 CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. none
SUPERFAMILY 25 70 46 SSF88713 none none IPR011330
Phobius 1 4 4 SIGNAL_PEPTIDE_N_REGION none N-terminal region of a signal peptide. none
PANTHER 1 76 76 PTHR11607:SF18 none none none
Phobius 104 122 19 TRANSMEMBRANE none Region of a membrane-bound protein predicted to be embedded in the membrane. none
Phobius 123 133 11 NON_CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. none
Phobius 5 12 8 SIGNAL_PEPTIDE_H_REGION none Hydrophobic region of a signal peptide. none
PANTHER 1 76 76 PTHR11607 none none none

5 Localization

Analysis Start End Length
TMHMM 67 89 22
TMHMM 5 27 22
SignalP_EUK 1 17 16
TMHMM 135 154 19
TMHMM 101 120 19

0 Qtllist

0 Targeting