| Analysis | rpsblast_cdd | Start | 36 |
| End | 67 | Strand | + |
| Length | 32 | Note | none |
| Hit Coverage | 11.51 | Hit Length | 278 |
| Hit Pident | 90.62 | E Val | 2e-16 |
| Hit Description | cd10810 GH38N_AMII_LAM_like N-terminal catalytic domain of lysosomal alpha-mannosidase and similar proteins glycoside hydrolase family 38 (GH38). The subfamily is represented by lysosomal alpha-mannosidase (LAM Man2B1 EC 3.2.1.114) which is a broad specificity exoglycosidase hydrolyzing all known alpha 1 2- alpha 1 3- and alpha 1 6-mannosidic linkages from numerous high mannose type oligosaccharides. LAM is expressed in all tissues and in many species. In mammals the absence of LAM can cause the autosomal recessive disease alpha-mannosidosis. LAM has an acidic pH optimum at 4.0-4.5. It is stimulated by zinc ion and is inhibited by cobalt ion and plant alkaloids such as swainsonine (SW). LAM catalyzes hydrolysis by a double displacement mechanism in which a glycosyl-enzyme intermediate is formed and hydrolyzed via oxacarbenium ion-like transition states. A carboxylic acid in the active site acts as the catalytic nucleophile in the formation of the covalent intermediate while a second carboxylic acid acts as a general acid catalyst. The same residue is thought to assist in the hydrolysis (deglycosylation) step this time acting as a general base. | Hit Pcons | 0.00 |
| Name | Qrob_P0551070.2 |
| Protein Identifier |
Organism . Name |
Score | Score Type | Protein Description | Alias (in v1) | Code Enzyme | Gene Prediction Quality |
|---|---|---|---|---|---|---|---|
| Qrob_P0551070.2 | Quercus robur | 94.0 | egn | (M=7) 3.2.1.24 - Alpha-mannosidase. | EC:3.2.1.24 | validated |
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