| Analysis | rpsblast_cdd | Start | 36 |
| End | 65 | Strand | + |
| Length | 30 | Note | none |
| Hit Coverage | 11.63 | Hit Length | 258 |
| Hit Pident | 66.67 | E Val | 4e-09 |
| Hit Description | cd00451 GH38N_AMII_euk N-terminal catalytic domain of eukaryotic class II alpha-mannosidases glycoside hydrolase family 38 (GH38). The family corresponds to a group of eukaryotic class II alpha-mannosidases (AlphaMII) which contain Golgi alpha-mannosidases II (GMII) the major broad specificity lysosomal alpha-mannosidases (LAM MAN2B1) the noval core-specific lysosomal alpha 1 6-mannosidases (Epman MAN2B2) and similar proteins. GMII catalyzes the hydrolysis of the terminal both alpha-1 3-linked and alpha-1 6-linked mannoses from the high-mannose oligosaccharide GlcNAc(Man)5(GlcNAc)2 to yield GlcNAc(Man)3(GlcNAc)2 (GlcNAc N-acetylglucosmine) which is the committed step of complex N-glycan synthesis. LAM is a broad specificity exoglycosidase hydrolyzing all known alpha 1 2- alpha 1 3- and alpha 1 6-mannosidic linkages from numerous high mannose type oligosaccharides. Different from LAM Epman can efficiently cleave only the alpha 1 6-linked mannose residue from (Man)3GlcNAc but not (Man)3(GlcNAc)2 or other larger high mannose oligosaccharides in the core of N-linked glycans. Members in this family are retaining glycosyl hydrolases of family GH38 that employs a two-step mechanism involving the formation of a covalent glycosyl enzyme complex. Two carboxylic acids positioned within the active site act in concert: one as a catalytic nucleophile and the other as a general acid/base catalyst. | Hit Pcons | 10.00 |
| Name | Qrob_P0551070.2 |
| Protein Identifier |
Organism . Name |
Score | Score Type | Protein Description | Alias (in v1) | Code Enzyme | Gene Prediction Quality |
|---|---|---|---|---|---|---|---|
| Qrob_P0551070.2 | Quercus robur | 94.0 | egn | (M=7) 3.2.1.24 - Alpha-mannosidase. | EC:3.2.1.24 | validated |
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