Protein : Qrob_P0492490.2 Q. robur
Protein Identifier  ? Qrob_P0492490.2 Organism . Name  Quercus robur
Score  76.1 Score Type  egn
Protein Description  (M=1) K10884 - ATP-dependent DNA helicase 2 subunit 1 Code Enzyme  EC:3.6.4.12
Gene Prediction Quality  validated Protein length 

Sequence

Length: 424  
Kegg Orthology  K10884
Paste the following link
Lists
This Protein isn't in any lists. Upload a list.

Sequence Feature Displayer

Protein Sequence Displayer

J Browse Displayer

0 Synonyms

7 GO Terms

Identifier Name Description
GO:0003677 DNA binding Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid).
GO:0005634 nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
GO:0003684 damaged DNA binding Interacting selectively and non-covalently with damaged DNA.
GO:0000723 telomere maintenance Any process that contributes to the maintenance of proper telomeric length and structure by affecting and monitoring the activity of telomeric proteins and the length of telomeric DNA. These processes includes those that shorten and lengthen the telomeric DNA sequences.
GO:0042162 telomeric DNA binding Interacting selectively and non-covalently with a telomere, a specific structure at the end of a linear chromosome required for the integrity and maintenance of the end.
GO:0006303 double-strand break repair via nonhomologous end joining The repair of a double-strand break in DNA in which the two broken ends are rejoined with little or no sequence complementarity. Information at the DNA ends may be lost due to the modification of broken DNA ends. This term covers instances of separate pathways, called classical (or canonical) and alternative nonhomologous end joining (C-NHEJ and A-NHEJ). These in turn may further branch into sub-pathways, but evidence is still unclear.
GO:0043564 Ku70:Ku80 complex Heterodimeric protein complex composed of a 70 kDa and a 80 kDa subunit, binds DNA through a channel formed by the heterodimer. Functions in DNA double stranded break repair, chromosome maintenance, transcription regulation, V(D)J recombination, and activation of DNA-PK.

30 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|pop:POPTR_0011s10870g 1 423 + 423 none 67.36 628 83.92 0.0 POPTRDRAFT_235497 Ku70-like family protein
blastp_kegg lcl|tcc:TCM_031961 1 423 + 423 none 67.36 628 78.49 0.0 KU70 isoform 1
blastp_kegg lcl|sot:102580271 1 423 + 423 none 99.53 425 77.54 0.0 ATP-dependent DNA helicase 2 subunit KU70-like
blastp_kegg lcl|cit:102627949 1 423 + 423 none 67.90 623 82.51 0.0 ATP-dependent DNA helicase 2 subunit KU70-like
blastp_kegg lcl|pmum:103322539 1 423 + 423 none 67.36 628 80.61 0.0 ATP-dependent DNA helicase 2 subunit KU70
blastp_kegg lcl|pper:PRUPE_ppa002852mg 1 423 + 423 none 67.36 628 80.38 0.0 hypothetical protein
blastp_kegg lcl|rcu:RCOM_0534680 1 423 + 423 Gaps:1 67.41 626 82.94 0.0 ku P70 DNA helicase putative
blastp_kegg lcl|cic:CICLE_v10019318mg 1 423 + 423 none 67.90 623 82.03 0.0 hypothetical protein
blastp_kegg lcl|vvi:100265198 23 423 + 401 Gaps:1 64.21 623 83.00 0.0 ATP-dependent DNA helicase 2 subunit KU70-like
blastp_kegg lcl|sly:101260670 1 423 + 423 none 67.79 624 76.83 0.0 ATP-dependent DNA helicase 2 subunit KU70-like
blastp_pdb 1jey_A 24 423 + 400 Gaps:24 63.38 609 33.16 3e-44 mol:protein length:609 Ku70
blastp_pdb 1jeq_A 24 423 + 400 Gaps:24 63.38 609 33.16 3e-44 mol:protein length:609 KU70
blastp_uniprot_sprot sp|Q9FQ08|KU70_ARATH 1 423 + 423 Gaps:1 68.28 621 71.93 0.0 ATP-dependent DNA helicase 2 subunit KU70 OS Arabidopsis thaliana GN KU70 PE 1 SV 1
blastp_uniprot_sprot sp|Q7F1M0|KU70_ORYSJ 1 423 + 423 Gaps:2 67.47 624 69.12 0.0 ATP-dependent DNA helicase 2 subunit KU70 OS Oryza sativa subsp. japonica GN KU70 PE 1 SV 1
blastp_uniprot_sprot sp|O93257|XRCC6_CHICK 26 423 + 398 Gaps:27 60.92 632 33.25 3e-48 X-ray repair cross-complementing protein 5 OS Gallus gallus GN XRCC6 PE 2 SV 1
blastp_uniprot_sprot sp|P12956|XRCC6_HUMAN 24 423 + 400 Gaps:24 63.38 609 33.16 1e-43 X-ray repair cross-complementing protein 6 OS Homo sapiens GN XRCC6 PE 1 SV 2
blastp_uniprot_sprot sp|Q54MA9|KU70_DICDI 21 423 + 403 Gaps:51 45.54 909 29.71 5e-42 ATP-dependent DNA helicase ku70 OS Dictyostelium discoideum GN ku70 PE 3 SV 1
blastp_uniprot_sprot sp|P23475|XRCC6_MOUSE 24 423 + 400 Gaps:26 63.49 608 32.12 9e-40 X-ray repair cross-complementing protein 6 OS Mus musculus GN Xrcc6 PE 1 SV 5
blastp_uniprot_sprot sp|Q2MHH3|KU70_ASPSO 25 422 + 398 Gaps:45 63.36 655 25.30 3e-32 ATP-dependent DNA helicase II subunit 1 OS Aspergillus sojae GN ku70 PE 3 SV 1
blastp_uniprot_sprot sp|Q2MHH4|KU70_ASPOR 25 422 + 398 Gaps:45 63.36 655 25.06 3e-32 ATP-dependent DNA helicase II subunit 1 OS Aspergillus oryzae (strain ATCC 42149 / RIB 40) GN ku70 PE 3 SV 1
blastp_uniprot_sprot sp|Q26228|KU70_RHIAP 31 423 + 393 Gaps:35 62.33 600 28.07 6e-32 ATP-dependent DNA helicase 2 subunit 1 OS Rhipicephalus appendiculatus GN ku70 PE 2 SV 1
blastp_uniprot_sprot sp|Q7SA95|KU70_NEUCR 12 420 + 409 Gaps:46 64.03 645 25.67 7e-29 ATP-dependent DNA helicase II subunit 1 OS Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) GN mus-51 PE 3 SV 2
rpsblast_cdd gnl|CDD|129668 31 423 + 393 Gaps:29 65.07 584 33.42 6e-51 TIGR00578 ku70 ATP-dependent DNA helicase II 70 kDa subunit (ku70). Proteins in this family are involved in non-homologous end joining a process used for the repair of double stranded DNA breaks. This family is based on the phylogenomic analysis of JA Eisen (1999 Ph.D. Thesis Stanford University). Cutoff does not detect the putative ku70 homologs in yeast.
rpsblast_cdd gnl|CDD|190730 31 266 + 236 Gaps:26 99.55 221 35.45 7e-36 pfam03731 Ku_N Ku70/Ku80 N-terminal alpha/beta domain. The Ku heterodimer (composed of Ku70 and Ku80) contributes to genomic integrity through its ability to bind DNA double-strand breaks and facilitate repair by the non-homologous end-joining pathway. This is the amino terminal alpha/beta domain. This domain only makes a small contribution to the dimer interface. The domain comprises a six stranded beta sheet of the Rossman fold.
rpsblast_cdd gnl|CDD|29231 28 259 + 232 Gaps:28 100.00 218 36.24 3e-33 cd01458 vWA_ku Ku70/Ku80 N-terminal domain. The Ku78 heterodimer (composed of Ku70 and Ku80) contributes to genomic integrity through its ability to bind DNA double-strand breaks (DSB) in a preferred orientation. DSB's are repaired by either homologues recombination or non-homologues end joining and facilitate repair by the non-homologous end-joining pathway (NHEJ). The Ku heterodimer is required for accurate process that tends to preserve the sequence at the junction. Ku78 is found in all three kingdoms of life. However only the eukaryotic proteins have a vWA domain fused to them at their N-termini. The vWA domain is not involved in DNA binding but may very likey mediate Ku78's interactions with other proteins. Members of this subgroup lack the conserved MIDAS motif..
rpsblast_cdd gnl|CDD|63880 264 423 + 160 Gaps:8 58.54 287 33.33 7e-33 cd00788 KU70 Ku-core domain Ku70 subfamily Ku70 is a subunit of the Ku protein which plays a key role in multiple nuclear processes such as DNA repair chromosome maintenance transcription regulation and V(D)J recombination. The mechanism underlying the regulation of all the diverse functions of Ku is still unclear although it seems that Ku is a multifunctional protein that works in the nuclei. In mammalian cells the Ku heterodimer recruits the catalytic subunit of DNA-dependent protein kinase (DNA-PK) which is dependent on its association with the Ku70/80 heterodimer bound to DNA for its protein kinase activity..
rpsblast_cdd gnl|CDD|190405 271 423 + 153 Gaps:3 78.00 200 32.69 4e-31 pfam02735 Ku Ku70/Ku80 beta-barrel domain. The Ku heterodimer (composed of Ku70 and Ku80) contributes to genomic integrity through its ability to bind DNA double-strand breaks and facilitate repair by the non-homologous end-joining pathway. This is the central DNA-binding beta-barrel domain. This domain is found in both the Ku70 and Ku80 proteins that form a DNA binding heterodimer.
rpsblast_cdd gnl|CDD|128831 317 423 + 107 Gaps:2 77.86 140 38.53 3e-25 smart00559 Ku78 Ku70 and Ku80 are 70kDa and 80kDa subunits of the Lupus Ku autoantigen. This is a single stranded DNA- and ATP-depedent helicase that has a role in chromosome translocation. This is a domain of unknown function C-terminal to its von Willebrand factor A domain that also occurs in bacterial hypothetical proteins.
rpsblast_cdd gnl|CDD|63879 264 423 + 160 Gaps:3 59.19 272 27.33 2e-23 cd00594 KU Ku-core domain includes the central DNA-binding beta-barrels polypeptide rings and the C-terminal arm of Ku proteins. The Ku protein consists of two tightly associated homologous subunits Ku70 and Ku80 and was originally identified as an autoantigen recognized by the sera of patients with an autoimmunity disease. In eukaryotes the Ku heterodimer contributes to genomic integrity through its ability to bind DNA double-strand breaks and facilitate repair by non-homologous end-joining. The bacterial Ku homologs does not contain the conserved N-terminal extension that is present in the eukaryotic Ku protein..
rpsblast_kog gnl|CDD|37538 11 423 + 413 Gaps:26 66.94 602 23.82 3e-34 KOG2327 KOG2327 KOG2327 DNA-binding subunit of a DNA-dependent protein kinase (Ku70 autoantigen) [Replication recombination and repair].

10 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
Gene3D 30 259 230 G3DSA:3.40.50.410 none none IPR002035
SUPERFAMILY 264 422 159 SSF100939 none none IPR016194
Pfam 31 265 235 PF03731 "Reactome:REACT_216","Reactome:REACT_6185" Ku70/Ku80 N-terminal alpha/beta domain IPR005161
SMART 317 423 107 SM00559 "Reactome:REACT_216","Reactome:REACT_6185" Ku70 and Ku80 are 70kDa and 80kDa subunits of the Lupus Ku autoantigen IPR006164
Pfam 271 423 153 PF02735 "Reactome:REACT_216","Reactome:REACT_6185" Ku70/Ku80 beta-barrel domain IPR006164
TIGRFAM 26 423 398 TIGR00578 none ku70: ATP-dependent DNA helicase II, 70 kDa subunit (ku70) IPR006165
PANTHER 1 423 423 PTHR12604:SF2 none none IPR006165
PANTHER 1 423 423 PTHR12604 none none none
Gene3D 322 423 102 G3DSA:2.40.290.10 none none IPR016194
SUPERFAMILY 28 262 235 SSF53300 none none IPR002035

0 Localization

16 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Champenoux_2015_nPriLBD_3P Qrob_Chr10 10 v_15000_157 v_15000_310 15,68 15,9 15,91 lod 2.4 5.5
Bourran2_2014_nSecLBD_3P Qrob_Chr08 8 s_1BN2OD_551 s_1B5AYF_599 17,17 0 43,51 lod 1,9229 4,4
Bourran2_2014_nLBD*_3P Qrob_Chr08 8 v_5216_549 v_11837_70 12,25 0 35,55 lod 2,5951 6
Bourran2_2014_nP*_3P Qrob_Chr08 8 v_5216_549 v_11837_70 12,19 0 31,97 lod 2,8472 6
Bourran2_2002_QTL11_peak_Bud_burst_A4 Qrob_Chr06 6 s_1C41PA_791 s_1AM1AV_1141 19,17 0 34,57 lod 2,3 2,9
Bourran2_2014_aSeqBC_3P Qrob_Chr06 6 v_506_189 v_686_77 30,72 13,58 43,48 lod 2,2746 6,3
Bourran2_2014_aSeqBC*_A4 Qrob_Chr06 6 s_2F5MK3_712 v_444_355 27,13 14,86 39,46 lod 3,7847 9,8
Bourran2_2014_nEpis*_3P Qrob_Chr08 8 s_1DA4QW_688 s_1DNI7D_820 17,96 0 37,75 lod 2,9745 7,5
Bourran2_2014_nEpis*_A4 Qrob_Chr07 7 v_12400_446 s_1BPEBU_1211 6,93 0 15,13 lod 4,7411 11
Bourran2_2014_nFork*_3P Qrob_Chr06 6 v_12930_125 s_1AMZEI_909 34,28 7,43 41,48 lod 2,4044 5,5
Bourran2_2014_nLBD_A4 Qrob_Chr06 6 v_12930_125 s_1AMZEI_909 37,41 9,8 50,1 lod 1,9524 4,1
Bourran2_2014_nPriBD_A4 Qrob_Chr06 6 v_12930_125 s_1AMZEI_909 34,51 3,36 51,86 lod 1,6747 3,9
Bourran2_2014_nSecLBD_A4 Qrob_Chr07 7 v_8327_222 s_1A4WGY_363 16,04 0 44,69 lod 2,6373 6,5
Bourran2_2014_vEpiBC_A4 Qrob_Chr06 6 v_12930_125 s_1AMZEI_909 37,55 14,41 50,01 lod 1,7882 4,8
Champenoux_2015_nEpis_3P Qrob_Chr11 11 s_1DG9PM_867 s_1BZ083_1312 26,53 25,47 27,72 lod 4.4 8.9
Champenoux_2015_nP_3P Qrob_Chr06 6 s_1A386O_228 s_1AYZFS_603 27,03 26,47 27,34 lod 2.8 7.2

0 Targeting