Protein : Qrob_P0296590.2 Q. robur
Protein Identifier  ? Qrob_P0296590.2 Organism . Name  Quercus robur
Score  100.0 Score Type  egn
Protein Description  (M=1) 2.8.4.3 - tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase. Code Enzyme  EC:2.8.4.3
Gene Prediction Quality  validated Protein length 

Sequence

Length: 609  
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0 Synonyms

6 GO Terms

Identifier Name Description
GO:0003824 catalytic activity Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic.
GO:0009451 RNA modification The covalent alteration of one or more nucleotides within an RNA molecule to produce an RNA molecule with a sequence that differs from that coded genetically.
GO:0016740 transferase activity Catalysis of the transfer of a group, e.g. a methyl group, glycosyl group, acyl group, phosphorus-containing, or other groups, from one compound (generally regarded as the donor) to another compound (generally regarded as the acceptor). Transferase is the systematic name for any enzyme of EC class 2.
GO:0051539 4 iron, 4 sulfur cluster binding Interacting selectively and non-covalently with a 4 iron, 4 sulfur (4Fe-4S) cluster; this cluster consists of four iron atoms, with the inorganic sulfur atoms found between the irons and acting as bridging ligands.
GO:0051536 iron-sulfur cluster binding Interacting selectively and non-covalently with an iron-sulfur cluster, a combination of iron and sulfur atoms.
GO:0043412 macromolecule modification The covalent alteration of one or more monomeric units in a polypeptide, polynucleotide, polysaccharide, or other biological macromolecule, resulting in a change in its properties.

40 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|pper:PRUPE_ppa002826mg 14 608 + 595 Gaps:18 95.87 629 80.43 0.0 hypothetical protein
blastp_kegg lcl|vvi:100266017 12 605 + 594 Gaps:6 94.89 626 81.14 0.0 CDK5RAP1-like protein-like
blastp_kegg lcl|tcc:TCM_000288 1 608 + 608 Gaps:20 100.00 616 80.52 0.0 CDK5RAP1-like protein isoform 1
blastp_kegg lcl|pop:POPTR_0007s13510g 14 606 + 593 Gaps:20 97.43 623 80.56 0.0 POPTRDRAFT_869356 hypothetical protein
blastp_kegg lcl|pmum:103340153 14 608 + 595 Gaps:16 95.87 629 80.10 0.0 CDK5RAP1-like protein
blastp_kegg lcl|cit:102622909 1 594 + 594 Gaps:25 88.55 690 78.72 0.0 CDK5RAP1-like protein-like
blastp_kegg lcl|fve:101309579 8 606 + 599 Gaps:3 96.92 617 79.93 0.0 CDK5RAP1-like protein-like
blastp_kegg lcl|mdm:103401098 14 608 + 595 Gaps:10 95.71 630 78.77 0.0 CDK5RAP1-like protein
blastp_kegg lcl|gmx:100812447 70 604 + 535 Gaps:1 87.40 611 85.21 0.0 CDK5RAP1-like protein-like
blastp_kegg lcl|gmx:100799544 1 603 + 603 Gaps:27 99.51 611 77.80 0.0 CDK5RAP1-like protein-like
blastp_pdb 2qgq_H 256 585 + 330 Gaps:39 95.72 304 31.96 6e-30 mol:protein length:304 Protein TM_1862
blastp_pdb 2qgq_G 256 585 + 330 Gaps:39 95.72 304 31.96 6e-30 mol:protein length:304 Protein TM_1862
blastp_pdb 2qgq_F 256 585 + 330 Gaps:39 95.72 304 31.96 6e-30 mol:protein length:304 Protein TM_1862
blastp_pdb 2qgq_E 256 585 + 330 Gaps:39 95.72 304 31.96 6e-30 mol:protein length:304 Protein TM_1862
blastp_pdb 2qgq_D 256 585 + 330 Gaps:39 95.72 304 31.96 6e-30 mol:protein length:304 Protein TM_1862
blastp_pdb 2qgq_C 256 585 + 330 Gaps:39 95.72 304 31.96 6e-30 mol:protein length:304 Protein TM_1862
blastp_pdb 2qgq_B 256 585 + 330 Gaps:39 95.72 304 31.96 6e-30 mol:protein length:304 Protein TM_1862
blastp_pdb 2qgq_A 256 585 + 330 Gaps:39 95.72 304 31.96 6e-30 mol:protein length:304 Protein TM_1862
blastp_uniprot_sprot sp|Q8H0V1|CK5P1_ARATH 50 594 + 545 Gaps:15 86.56 640 77.08 0.0 CDK5RAP1-like protein OS Arabidopsis thaliana GN At4g36390 PE 2 SV 1
blastp_uniprot_sprot sp|Q2R1U4|CK5P1_ORYSJ 66 603 + 538 Gaps:7 89.17 600 78.13 0.0 CDK5RAP1-like protein OS Oryza sativa subsp. japonica GN Os11g0592800 PE 2 SV 1
blastp_uniprot_sprot sp|Q9JLH6|CK5P1_RAT 54 590 + 537 Gaps:24 90.61 586 53.30 0.0 CDK5 regulatory subunit-associated protein 1 OS Rattus norvegicus GN Cdk5rap1 PE 1 SV 1
blastp_uniprot_sprot sp|Q8BTW8|CK5P1_MOUSE 61 596 + 536 Gaps:25 90.31 588 52.73 1e-180 CDK5 regulatory subunit-associated protein 1 OS Mus musculus GN Cdk5rap1 PE 2 SV 2
blastp_uniprot_sprot sp|Q96SZ6|CK5P1_HUMAN 61 596 + 536 Gaps:46 90.52 601 51.29 8e-176 CDK5 regulatory subunit-associated protein 1 OS Homo sapiens GN CDK5RAP1 PE 1 SV 2
blastp_uniprot_sprot sp|Q9VGZ1|CK5P1_DROME 98 595 + 498 Gaps:14 83.36 583 52.67 2e-173 CDK5RAP1-like protein OS Drosophila melanogaster GN CG6345 PE 2 SV 1
blastp_uniprot_sprot sp|Q54KV4|CK5P1_DICDI 88 595 + 508 Gaps:42 82.37 607 49.00 9e-159 CDK5RAP1-like protein OS Dictyostelium discoideum GN cdk5rap1 PE 3 SV 1
blastp_uniprot_sprot sp|Q2RZF8|MIAB_SALRD 98 592 + 495 Gaps:50 88.87 503 52.57 4e-153 (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB OS Salinibacter ruber (strain DSM 13855 / M31) GN miaB PE 3 SV 2
blastp_uniprot_sprot sp|Q09316|CK5P1_CAEEL 64 546 + 483 Gaps:31 87.39 547 47.91 3e-147 CDK5RAP1-like protein OS Caenorhabditis elegans GN F25B5.5 PE 3 SV 1
blastp_uniprot_sprot sp|B3ES11|MIAB_AMOA5 85 583 + 499 Gaps:41 94.65 486 51.96 7e-146 (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB OS Amoebophilus asiaticus (strain 5a2) GN miaB PE 3 SV 1
rpsblast_cdd gnl|CDD|184626 98 584 + 487 Gaps:47 94.22 467 53.18 1e-170 PRK14329 PRK14329 (dimethylallyl)adenosine tRNA methylthiotransferase Provisional.
rpsblast_cdd gnl|CDD|172816 98 590 + 493 Gaps:55 98.43 445 44.98 1e-122 PRK14340 PRK14340 (dimethylallyl)adenosine tRNA methylthiotransferase Provisional.

15 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
ProSiteProfiles 97 226 130 PS51449 none Methylthiotransferase N-terminal domain profile. IPR013848
PANTHER 333 597 265 PTHR11918 none none IPR023970
PANTHER 333 597 265 PTHR11918:SF43 none none none
ProSitePatterns 258 278 21 PS01278 none Methylthiotransferase radical SAM domain signature. IPR020612
PANTHER 32 315 284 PTHR11918:SF43 none none none
TIGRFAM 98 545 448 TIGR00089 none TIGR00089: radical SAM methylthiotransferase, MiaB/RimO family IPR005839
SMART 254 495 242 SM00729 none Elongator protein 3, MiaB family, Radical SAM IPR006638
PANTHER 32 315 284 PTHR11918 none none IPR023970
Pfam 509 585 77 PF01938 none TRAM domain IPR002792
Pfam 98 210 113 PF00919 none Uncharacterized protein family UPF0004 IPR013848
Gene3D 253 503 251 G3DSA:3.80.30.20 none none IPR023404
SUPERFAMILY 248 316 69 SSF102114 none none none
SUPERFAMILY 347 502 156 SSF102114 none none none
Pfam 258 455 198 PF04055 none Radical SAM superfamily IPR007197
ProSiteProfiles 509 586 78 PS50926 none TRAM domain profile. IPR002792

0 Localization

10 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Bourran2_2014_nSecLBD_3P Qrob_Chr08 8 s_1BN2OD_551 s_1B5AYF_599 17,17 0 43,51 lod 1,9229 4,4
Bourran2_2014_nLBD*_3P Qrob_Chr08 8 v_5216_549 v_11837_70 12,25 0 35,55 lod 2,5951 6
Bourran2_2014_aSeqBC_3P Qrob_Chr06 6 v_506_189 v_686_77 30,72 13,58 43,48 lod 2,2746 6,3
Bourran2_2014_nEpis*_3P Qrob_Chr08 8 s_1DA4QW_688 s_1DNI7D_820 17,96 0 37,75 lod 2,9745 7,5
Bourran2_2014_nEpis*_A4 Qrob_Chr07 7 v_12400_446 s_1BPEBU_1211 6,93 0 15,13 lod 4,7411 11
Bourran2_2014_nLBD_A4 Qrob_Chr06 6 v_12930_125 s_1AMZEI_909 37,41 9,8 50,1 lod 1,9524 4,1
Bourran2_2014_nPriBD_A4 Qrob_Chr06 6 v_12930_125 s_1AMZEI_909 34,51 3,36 51,86 lod 1,6747 3,9
Bourran2_2014_nSecLBD_A4 Qrob_Chr07 7 v_8327_222 s_1A4WGY_363 16,04 0 44,69 lod 2,6373 6,5
Bourran2_2014_vEpiBC_A4 Qrob_Chr06 6 v_12930_125 s_1AMZEI_909 37,55 14,41 50,01 lod 1,7882 4,8
Champenoux_2015_nEpis_3P Qrob_Chr11 11 s_1DG9PM_867 s_1BZ083_1312 26,53 25,47 27,72 lod 4.4 8.9

1 Targeting

Analysis Start End Length Location Reliability Signal Peptide Cut Off Mitochondrion Cut Off Network Signal Peptide Length
TargetP 1 65   Mitochondrion 2 0.044 0.894 NON-PLANT 65