Protein : Qrob_P0236660.2 Q. robur
Protein Identifier  ? Qrob_P0236660.2 Organism . Name  Quercus robur
Score  100.0 Score Type  egn
Protein Description  (M=1) K03249 - translation initiation factor eIF-3 subunit 5 Gene Prediction Quality  validated
Protein length 

Sequence

Length: 286  
Kegg Orthology  K03249
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0 Synonyms

4 GO Terms

Identifier Name Description
GO:0005515 protein binding Interacting selectively and non-covalently with any protein or protein complex (a complex of two or more proteins that may include other nonprotein molecules).
GO:0005737 cytoplasm All of the contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
GO:0003743 translation initiation factor activity Functions in the initiation of ribosome-mediated translation of mRNA into a polypeptide.
GO:0005852 eukaryotic translation initiation factor 3 complex A complex of several polypeptides that plays at least two important roles in protein synthesis: First, eIF3 binds to the 40S ribosome and facilitates loading of the Met-tRNA/eIF2.GTP ternary complex to form the 43S preinitiation complex. Subsequently, eIF3 apparently assists eIF4 in recruiting mRNAs to the 43S complex. The eIF3 complex contains five conserved core subunits, and may contain several additional proteins; the non-core subunits are thought to mediate association of the complex with specific sets of mRNAs.

35 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|cmo:103485557 1 285 + 285 none 100.00 285 87.02 0.0 eukaryotic translation initiation factor 3 subunit F
blastp_kegg lcl|pop:POPTR_0010s19940g 1 285 + 285 Gaps:2 100.00 287 87.80 0.0 POPTRDRAFT_659552 Eukaryotic translation initiation factor 3 subunit 5 family protein
blastp_kegg lcl|csv:101226011 1 285 + 285 none 100.00 285 86.67 0.0 eukaryotic translation initiation factor 3 subunit F-like
blastp_kegg lcl|csv:101205957 1 285 + 285 none 100.00 285 86.67 0.0 eukaryotic translation initiation factor 3 subunit F-like
blastp_kegg lcl|tcc:TCM_042592 1 285 + 285 Gaps:1 100.00 286 88.46 0.0 Eukaryotic translation initiation factor 2 isoform 2
blastp_kegg lcl|pper:PRUPE_ppa009579mg 1 285 + 285 Gaps:1 100.00 286 87.41 0.0 hypothetical protein
blastp_kegg lcl|rcu:RCOM_0592110 1 285 + 285 Gaps:2 100.00 287 86.76 1e-179 eukaryotic translation initiation factor 3f eif3f putative
blastp_kegg lcl|vvi:100249540 1 284 + 284 none 99.65 285 86.97 6e-179 eukaryotic translation initiation factor 3 subunit F-like
blastp_kegg lcl|pmum:103332752 1 285 + 285 Gaps:1 100.00 286 88.46 3e-178 eukaryotic translation initiation factor 3 subunit F
blastp_kegg lcl|mdm:103455106 1 285 + 285 Gaps:2 100.00 285 86.67 4e-176 eukaryotic translation initiation factor 3 subunit F-like
blastp_pdb 2o96_B 22 147 + 126 Gaps:6 73.03 178 36.15 2e-14 mol:protein length:178 26S proteasome non-ATPase regulatory subunit
blastp_pdb 2o96_A 22 147 + 126 Gaps:6 73.03 178 36.15 2e-14 mol:protein length:178 26S proteasome non-ATPase regulatory subunit
blastp_pdb 2o95_B 22 147 + 126 Gaps:6 69.52 187 36.15 2e-14 mol:protein length:187 26S proteasome non-ATPase regulatory subunit
blastp_pdb 2o95_A 22 147 + 126 Gaps:6 69.52 187 36.15 2e-14 mol:protein length:187 26S proteasome non-ATPase regulatory subunit
blastp_uniprot_sprot sp|O04202|EIF3F_ARATH 2 285 + 284 Gaps:7 99.32 293 79.73 2e-161 Eukaryotic translation initiation factor 3 subunit F OS Arabidopsis thaliana GN TIF3F1 PE 2 SV 1
blastp_uniprot_sprot sp|Q9DCH4|EIF3F_MOUSE 21 278 + 258 Gaps:6 72.58 361 41.98 1e-68 Eukaryotic translation initiation factor 3 subunit F OS Mus musculus GN Eif3f PE 1 SV 2
blastp_uniprot_sprot sp|Q4R5B8|EIF3F_MACFA 21 278 + 258 Gaps:6 72.58 361 41.98 1e-68 Eukaryotic translation initiation factor 3 subunit F OS Macaca fascicularis GN EIF3F PE 2 SV 1
blastp_uniprot_sprot sp|A5A6I3|EIF3F_PANTR 21 278 + 258 Gaps:6 72.58 361 41.98 1e-68 Eukaryotic translation initiation factor 3 subunit F OS Pan troglodytes GN EIF3F PE 2 SV 1
blastp_uniprot_sprot sp|O00303|EIF3F_HUMAN 21 278 + 258 Gaps:6 73.39 357 41.98 1e-68 Eukaryotic translation initiation factor 3 subunit F OS Homo sapiens GN EIF3F PE 1 SV 1
blastp_uniprot_sprot sp|Q54C49|EIF3F_DICDI 16 278 + 263 Gaps:9 94.37 284 42.16 2e-67 Eukaryotic translation initiation factor 3 subunit F OS Dictyostelium discoideum GN eif3f PE 3 SV 1
blastp_uniprot_sprot sp|B3M123|EI3F1_DROAN 14 278 + 265 Gaps:10 97.50 280 37.73 8e-60 Eukaryotic translation initiation factor 3 subunit F-1 OS Drosophila ananassae GN eIF3-S5-1 PE 3 SV 1
blastp_uniprot_sprot sp|Q1HR47|EIF3F_AEDAE 18 280 + 263 Gaps:11 94.77 287 38.60 3e-59 Eukaryotic translation initiation factor 3 subunit F OS Aedes aegypti GN eIF3-S5 PE 2 SV 1
blastp_uniprot_sprot sp|B4JGX4|EI3F1_DROGR 14 278 + 265 Gaps:10 97.50 280 37.00 7e-59 Eukaryotic translation initiation factor 3 subunit F-1 OS Drosophila grimshawi GN eIF3-S5-1 PE 3 SV 1
blastp_uniprot_sprot sp|Q295I4|EI3F1_DROPS 14 278 + 265 Gaps:10 97.50 280 36.63 8e-59 Eukaryotic translation initiation factor 3 subunit F-1 OS Drosophila pseudoobscura pseudoobscura GN eIF3-S5-1 PE 3 SV 1
rpsblast_cdd gnl|CDD|163695 21 278 + 258 Gaps:8 99.62 265 53.41 1e-103 cd08064 MPN_eIF3f Mpr1p Pad1p N-terminal (MPN) domains without catalytic isopeptidase activity found in eIF3f. Eukaryotic translation initiation factor 3 (eIF3) subunit F (eIF3F EIF3S5 eIF3-p47 eukaryotic translation initiation factor 3 subunit 5 epsilon 47kDa Mov34/MPN/PAD-1 family protein) is an evolutionarily non-conserved subunit of the functional core that comprises eIF3a eIF3b eIF3c eIF3e eIF3f and eIF3h and contains the MPN domain. However it lacks the canonical JAMM motif and therefore does not show catalytic isopeptidase activity. It has been shown that eIF3f mRNA expression is significantly decreased in many human tumors including pancreatic cancer and melanoma. EIF3f is a potent inhibitor of HIV-1 replication it mediates restriction of HIV-1 expression through several factors including the serine/arginine-rich (SR) protein 9G8 and cyclin-dependent kinase 11 (CDK11). EIF3f phosphorylation by CDK11 is important in regulating its function in translation and apoptosis. It enhances its association with the core eIF3 subunits during apoptosis suggesting that eIF3f may inhibit translation by increasing the binding to the eIF3 complex during apoptosis. Thus eIF3f may be an important negative regulator of cell growth and proliferation.
rpsblast_cdd gnl|CDD|163693 22 274 + 253 Gaps:10 93.21 280 31.80 3e-32 cd08062 MPN_RPN7_8 Mpr1p Pad1p N-terminal (MPN) domains without catalytic isopeptidase activity found in 19S proteasomal subunits Rpn7 and Rpn8. This family includes lid subunits of the 26 S proteasome regulatory particles Rpn7 (PSMD7 proteasome 26S non-ATPase subunit 7 p44) and Rpn8 (PSMD8 proteasome 26S non-ATPase subunit 8 p40 Mov34). Rpn7 is known to be critical for the integrity of the 26 S proteasome complex by establishing a correct lid structure. It is necessary for the incorporation/anchoring of Rpn3 and Rpn12 to the lid and essential for viability and normal mitosis. Rpn7 and Rpn8 are ATP-independent components of the 19S regulator subunit and contain the MPN structural motif on its N-terminal region. However while they show a typical MPN metalloprotease fold they lack the canonical JAMM motif and therefore do not show catalytic isopeptidase activity. It is suggested that Rpn7 function is primarily structural.
rpsblast_cdd gnl|CDD|178783 22 279 + 258 Gaps:17 88.12 303 32.21 9e-30 PLN03246 PLN03246 26S proteasome regulatory subunit Provisional.
rpsblast_cdd gnl|CDD|163688 20 177 + 158 Gaps:15 100.00 157 30.57 9e-28 cd08057 MPN_euk_non_mb Mpr1p Pad1p N-terminal (MPN) domains without catalytic isopeptidase activity (non metal-binding) eukaryotic. This family contains MPN (also known as Mov34 PAD-1 JAMM JAB MPN+) domains variants lacking key residues in the JAB1/MPN/Mov34 metalloenzyme (JAMM) motif and are unable to coordinate a metal ion. Comparisons of key catalytic and metal binding residues explain why the MPN-containing proteins Rpn7/PSMD7 Rpn8/PSMD8 CSN6 Prp8p and the translation initiation factor 3 subunits f and h do not show catalytic isopeptidase activity. It has been proposed that the MPN domain in these proteins has a primarily structural function. Rpn7 is known to be critical for the integrity of the 26S proteasome complex by establishing a correct lid structure. It is necessary for the incorporation/anchoring of Rpn3 and Rpn12 to the lid and essential for viability and normal mitosis. CSN6 is a highly conserved protein complex with diverse functions including several important intracellular pathways such as the ubiquitin/proteasome system DNA repair cell cycle developmental changes and some aspects of immune responses. It cleaves ubiquitin-like protein Nedd8 (neural precursor cell expressed developmentally downregulated 8)) in the cullin 1 in cells. EIF3f s a potent inhibitor of HIV-1 replication as well as an important negative regulator of cell growth and proliferation. EIF3h regulates cell growth and viability and that over-expression of the gene may provide growth advantage to prostate breast and liver cancer cells.
rpsblast_cdd gnl|CDD|201771 16 125 + 110 Gaps:7 100.00 117 37.61 1e-24 pfam01398 JAB JAB1/Mov34/MPN/PAD-1 ubiquitin protease. Members of this family are found in proteasome regulatory subunits eukaryotic initiation factor 3 (eIF3) subunits and regulators of transcription factors. This family is also known as the MPN domain and PAD-1-like domain JABP1 domain or JAMM domain. These are metalloenzymes that function as the ubiquitin isopeptidase/ deubiquitinase in the ubiquitin-based signaling and protein turnover pathways in eukaryotes. Versions of the domain in prokaryotic cognates of the ubiquitin-modification pathway are predicted to have a similar role.
rpsblast_cdd gnl|CDD|163694 18 276 + 259 Gaps:24 94.10 288 30.63 2e-24 cd08063 MPN_CSN6 Mpr1p Pad1p N-terminal (MPN) domains without catalytic isopeptidase activity found in COP9 signalosome complex subunit 6. CSN6 (COP9 signalosome subunit 6 COP9 subunit 6 MOV34 homolog 34 kD) is one of the eight subunits of COP9 signalosome a highly conserved protein complex with diverse functions including several important intracellular pathways such as the ubiquitin/proteasome system DNA repair cell cycle developmental changes and some aspects of immune responses. CSN6 is an MPN-domain protein that directly interacts with the MPN+-domain subunit CSN5. It is cleaved during apoptosis by activated caspases. CSN6 processing occurs in CSN/CRL (cullin-RING Ub ligase) complexes and is followed by the cleavage of Rbx1 the direct interaction partner of CSN6. CSN6 cleavage enhances CSN-mediated deneddylating activity (i.e. cleavage of ubiquitin-like protein Nedd8 (neural precursor cell expressed developmentally downregulated 8)) in the cullin 1 in cells. The cleavage of Rbx1 and increased deneddylation of cullins inactivate CRLs and presumably stabilize pro-apoptotic factors for final apoptotic steps. While CSN6 shows a typical MPN metalloprotease fold it lacks the canonical JAMM motif and therefore does not show catalytic isopeptidase activity.

6 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
SMART 19 150 132 SM00232 none JAB/MPN domain IPR000555
PANTHER 14 285 272 PTHR10540:SF6 none none none
Hamap 18 281 264 MF_03005 none Eukaryotic translation initiation factor 3 subunit F [EIF3F]. IPR027531
PANTHER 14 285 272 PTHR10540 none none none
Pfam 172 279 108 PF13012 none Maintenance of mitochondrial structure and function IPR024969
Pfam 16 121 106 PF01398 none JAB1/Mov34/MPN/PAD-1 ubiquitin protease IPR000555

0 Localization

1 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Bourran2_2015_nEpiBC_3P Qrob_Chr12 12 s_1B73S5_217 v_7050_211 28,31 26,37 28,45 lod 4.5 11.6

0 Targeting