Protein : Qrob_P0176290.2 Q. robur
Protein Identifier  ? Qrob_P0176290.2 Organism . Name  Quercus robur
Score  100.0 Score Type  egn
Protein Description  (M=1) KOG2630//KOG2631 - Enolase-phosphatase E-1 [Amino acid transport and metabolism]. // Class II aldolase/adducin N-terminal domain protein [Carbohydrate transport and metabolism]. Gene Prediction Quality  validated
Protein length 

Sequence

Length: 526  
Kegg Orthology  K16054
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0 Synonyms

5 GO Terms

Identifier Name Description
GO:0005737 cytoplasm All of the contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
GO:0046872 metal ion binding Interacting selectively and non-covalently with any metal ion.
GO:0000287 magnesium ion binding Interacting selectively and non-covalently with magnesium (Mg) ions.
GO:0019509 L-methionine biosynthetic process from methylthioadenosine The generation of L-methionine (2-amino-4-(methylthio)butanoic acid) from methylthioadenosine.
GO:0043874 acireductone synthase activity Catalysis of the reactions: 5-(methylthio)-2,3-dioxopentyl phosphate + H2O = 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + phosphate; (1a) 5-(methylthio)-2,3-dioxopentyl phosphate = 2-hydroxy-5-(methylthio)-3-oxopent-1-enyl phosphate; (1b) 2-hydroxy-5-(methylthio)-3-oxopent-1-enyl phosphate + H2O = 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + phosphate.

40 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|pper:PRUPE_ppa004202mg 6 525 + 520 Gaps:1 99.62 523 84.84 0.0 hypothetical protein
blastp_kegg lcl|tcc:TCM_031540 1 525 + 525 Gaps:2 100.00 527 83.49 0.0 Bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 putative
blastp_kegg lcl|rcu:RCOM_0591060 11 525 + 515 Gaps:3 97.53 527 84.82 0.0 catalytic putative
blastp_kegg lcl|mdm:103422882 7 525 + 519 Gaps:2 100.00 521 82.92 0.0 probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1
blastp_kegg lcl|pop:POPTR_0001s40980g 17 525 + 509 Gaps:13 96.14 518 86.55 0.0 hypothetical protein
blastp_kegg lcl|vvi:100257258 6 525 + 520 Gaps:7 94.67 544 83.50 0.0 probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1-like
blastp_kegg lcl|cmo:103493479 1 525 + 525 Gaps:17 100.00 512 81.84 0.0 probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1
blastp_kegg lcl|mdm:103448693 4 525 + 522 Gaps:22 100.00 544 79.78 0.0 probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1
blastp_kegg lcl|cic:CICLE_v10007808mg 18 525 + 508 Gaps:5 86.13 591 83.50 0.0 hypothetical protein
blastp_kegg lcl|gmx:100817732 4 525 + 522 Gaps:2 100.00 522 80.08 0.0 probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1-like
blastp_pdb 1zs9_A 286 525 + 240 Gaps:9 93.10 261 45.68 2e-60 mol:protein length:261 E-1 ENZYME
blastp_pdb 1yns_A 286 525 + 240 Gaps:9 93.10 261 45.68 2e-60 mol:protein length:261 E-1 enzyme
blastp_pdb 2g80_D 287 522 + 236 Gaps:38 85.38 253 39.35 6e-32 mol:protein length:253 Protein UTR4
blastp_pdb 2g80_C 287 522 + 236 Gaps:38 85.38 253 39.35 6e-32 mol:protein length:253 Protein UTR4
blastp_pdb 2g80_B 287 522 + 236 Gaps:38 85.38 253 39.35 6e-32 mol:protein length:253 Protein UTR4
blastp_pdb 2g80_A 287 522 + 236 Gaps:38 85.38 253 39.35 6e-32 mol:protein length:253 Protein UTR4
blastp_pdb 2irp_B 34 237 + 204 Gaps:30 90.38 208 29.26 1e-11 mol:protein length:208 Putative aldolase class 2 protein aq_1979
blastp_pdb 2irp_A 34 237 + 204 Gaps:30 90.38 208 29.26 1e-11 mol:protein length:208 Putative aldolase class 2 protein aq_1979
blastp_uniprot_sprot sp|B9SQI7|MTBC_RICCO 11 525 + 515 Gaps:3 97.53 527 84.82 0.0 Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 OS Ricinus communis GN RCOM_0591060 PE 3 SV 1
blastp_uniprot_sprot sp|B9N1F9|MTBC_POPTR 17 525 + 509 Gaps:13 96.14 518 86.55 0.0 Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 OS Populus trichocarpa GN POPTR_0001s40980g PE 3 SV 2
blastp_uniprot_sprot sp|E0CSI1|MTBC1_VITVI 4 525 + 522 Gaps:7 100.00 517 83.17 0.0 Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1 OS Vitis vinifera GN VIT_19s0014g02480 PE 3 SV 2
blastp_uniprot_sprot sp|E0CTF3|MTBC2_VITVI 9 525 + 517 Gaps:3 97.93 531 74.04 0.0 Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 2 OS Vitis vinifera GN VIT_12s0028g03470 PE 2 SV 2
blastp_uniprot_sprot sp|Q9FN41|MTBC_ARATH 3 525 + 523 Gaps:17 99.80 507 78.26 0.0 Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 OS Arabidopsis thaliana GN At5g53850 PE 2 SV 1
blastp_uniprot_sprot sp|Q2R483|MTBC_ORYSJ 16 525 + 510 Gaps:8 97.30 518 72.02 0.0 Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 OS Oryza sativa subsp. japonica GN Os11g0484000 PE 2 SV 1
blastp_uniprot_sprot sp|B8BKI7|MTBC_ORYSI 16 525 + 510 Gaps:8 97.30 518 71.63 0.0 Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 OS Oryza sativa subsp. indica GN OsI_36120 PE 2 SV 1
blastp_uniprot_sprot sp|C6JS30|MTBC_SORBI 15 525 + 511 Gaps:13 97.49 517 70.04 0.0 Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 OS Sorghum bicolor GN SORBIDRAFT_0019s002010 PE 3 SV 1
blastp_uniprot_sprot sp|B4G0F3|MTBC_MAIZE 17 525 + 509 Gaps:19 97.10 517 70.12 0.0 Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 OS Zea mays PE 2 SV 1
blastp_uniprot_sprot sp|A9RBS1|MTBC_PHYPA 22 525 + 504 Gaps:14 97.03 505 68.16 0.0 Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 OS Physcomitrella patens subsp. patens GN PHYPADRAFT_111231 PE 3 SV 1
rpsblast_cdd gnl|CDD|163216 34 240 + 207 Gaps:18 100.00 193 35.75 5e-64 TIGR03328 salvage_mtnB methylthioribulose-1-phosphate dehydratase. Members of this family are the methylthioribulose-1-phosphate dehydratase of the methionine salvage pathway. This pathway allows methylthioadenosine left over from polyamine biosynthesis to be recycled to methionine.
rpsblast_cdd gnl|CDD|162490 284 512 + 229 Gaps:25 92.73 220 46.57 1e-58 TIGR01691 enolase-ppase 2 3-diketo-5-methylthio-1-phosphopentane phosphatase. This enzyme is the enolase-phosphatase of methionine salvage a pathway that regenerates methionine from methylthioadenosine (MTA). Adenosylmethionine (AdoMet) is a donor of different moieties for various processes including methylation reactions. Use of AdoMet for spermidine biosynthesis which leads to polyamine biosynthesis leaves MTA as a by-product that must be cleared. In Bacillus subtilis and related species this single protein is replaced by separate enzymes with enolase and phosphatase activities.

12 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
Gene3D 28 248 221 G3DSA:3.40.225.10 none none IPR001303
Gene3D 284 504 221 G3DSA:3.40.50.1000 none none IPR023214
SUPERFAMILY 284 525 242 SSF56784 none none IPR023214
Hamap 19 243 225 MF_03116 "KEGG:00270+4.2.1.109","MetaCyc:PWY-4361","UniPathway:UPA00904" Methylthioribulose-1-phosphate dehydratase [APIP]. IPR027514
Hamap 19 525 507 MF_03118 "KEGG:00270+4.2.1.109+3.1.3.77","MetaCyc:PWY-4361","UniPathway:UPA00904","UniPathway:UPA00904","UniPathway:UPA00904" Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1. IPR027505
TIGRFAM 284 522 239 TIGR01691 "KEGG:00270+3.1.3.77","UniPathway:UPA00904","UniPathway:UPA00904" enolase-ppase: 2,3-diketo-5-methylthio-1-phosphopentane phosphatase IPR023943
TIGRFAM 34 239 206 TIGR03328 "KEGG:00270+4.2.1.109","MetaCyc:PWY-4361","UniPathway:UPA00904" salvage_mtnB: methylthioribulose-1-phosphate dehydratase IPR017714
PANTHER 21 304 284 PTHR10640 "KEGG:00270+4.2.1.109","MetaCyc:PWY-4361","UniPathway:UPA00904";signature_desc=APAF1-INTERACTING PROTEIN none IPR017714
SMART 32 235 204 SM01007 none Class II Aldolase and Adducin N-terminal domain IPR001303
Pfam 286 500 215 PF13419 none Haloacid dehalogenase-like hydrolase IPR023214
Pfam 35 234 200 PF00596 none Class II Aldolase and Adducin N-terminal domain IPR001303
SUPERFAMILY 29 247 219 SSF53639 none none IPR001303

0 Localization

15 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Bourran2_2014_nLBD*_A4 Qrob_Chr08 8 v_12498_318 v_12364_308 34,91 16,12 53,62 lod 2,4961 5,2
Bourran2_2014_nSecLBD_3P Qrob_Chr08 8 s_1BN2OD_551 s_1B5AYF_599 17,17 0 43,51 lod 1,9229 4,4
Bourran2_2014_rEpiBC*_A4 Qrob_Chr08 8 v_12498_318 v_12364_308 35,77 14,11 55,31 lod 2,9413 6,2
Bourran2_2015_nEpiBC_3P Qrob_Chr12 12 s_1B73S5_217 v_7050_211 28,31 26,37 28,45 lod 4.5 11.6
Bourran2_2014_aSeqBC_A4 Qrob_Chr08 8 v_15999_278 v_AP13YL15_395 32,52 4,22 57,22 lod 2,7561 6,7
Bourran2_2014_nFork*_A4 Qrob_Chr08 8 PIE175 s_1CD7GJ_1398 31,22 5,24 57,24 lod 2,6724 6,8
Bourran2_2014_nLBD*_3P Qrob_Chr08 8 v_5216_549 v_11837_70 12,25 0 35,55 lod 2,5951 6
Bourran2_2014_nP*_3P Qrob_Chr08 8 v_5216_549 v_11837_70 12,19 0 31,97 lod 2,8472 6
Bourran2_2014_nPriLBD_3P Qrob_Chr08 8 v_5216_549 v_11837_70 12,36 0 30,43 lod 2,5806 5,1
Bourran2_2014_nPriLBD_A4 Qrob_Chr08 8 PIE175 v_9164_159 31,85 15,39 48,29 lod 2,8308 6,8
Bourran2_2015_rEpiBC_3P Qrob_Chr08 8 s_A9TNV_543 v_11837_70 9,93 9,83 11,15 lod 3.3 7.3
Champenoux_2015_nSeqBC_A4 Qrob_Chr08 8 v_AD7YD13_501 s_1A7IED_780 43,44 43,42 43,99 lod 3.7 8.9
NancyGreenhouseCO2_2001_ambient_elevated_leaf_cellulose_QTL3_d13Cf Qrob_Chr08 8 v_5216_549 v_11625_20 37.08 12,26 54,9 lod 6.5888 0.04
Bourran2_2014_nEpis*_3P Qrob_Chr08 8 s_1DA4QW_688 s_1DNI7D_820 17,96 0 37,75 lod 2,9745 7,5
Bourran2_2014_nPriBD_3P Qrob_Chr11 11 v_11486_194 s_1AT3E_2335 5,54 0,4 20,6 lod 2,6345 5,9

0 Targeting