Protein : Qrob_P0033990.2 Q. robur
Protein Identifier  ? Qrob_P0033990.2 Organism . Name  Quercus robur
Score  100.0 Score Type  egn
Protein Description  (M=1) PTHR22795 - SUPPRESSOR OF RNA POLYMERASE B SRB8-RELATED Gene Prediction Quality  validated
Protein length 

Sequence

Length: 2256  
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0 Synonyms

3 GO Terms

Identifier Name Description
GO:0001104 RNA polymerase II transcription cofactor activity Interacting selectively and non-covalently with an RNA polymerase II (RNAP II) regulatory transcription factor and also with the RNAP II basal transcription machinery in order to modulate transcription. Cofactors generally do not bind DNA, but rather mediate protein-protein interactions between regulatory transcription factors and the basal RNAP II transcription machinery.
GO:0006357 regulation of transcription from RNA polymerase II promoter Any process that modulates the frequency, rate or extent of transcription from an RNA polymerase II promoter.
GO:0016592 mediator complex A protein complex that interacts with the carboxy-terminal domain of the largest subunit of RNA polymerase II and plays an active role in transducing the signal from a transcription factor to the transcriptional machinery. The mediator complex is required for activation of transcription of most protein-coding genes, but can also act as a transcriptional corepressor. The Saccharomyces complex contains several identifiable subcomplexes: a head domain comprising Srb2, -4, and -5, Med6, -8, and -11, and Rox3 proteins; a middle domain comprising Med1, -4, and -7, Nut1 and -2, Cse2, Rgr1, Soh1, and Srb7 proteins; a tail consisting of Gal11p, Med2p, Pgd1p, and Sin4p; and a regulatory subcomplex comprising Ssn2, -3, and -8, and Srb8 proteins. Metazoan mediator complexes have similar modular structures and include homologs of yeast Srb and Med proteins.

19 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|pmum:103331984 1 2255 + 2255 Gaps:40 100.00 2277 72.99 0.0 mediator of RNA polymerase II transcription subunit 12
blastp_kegg lcl|vvi:100263628 1 2255 + 2255 Gaps:43 100.00 2272 72.76 0.0 uncharacterized LOC100263628
blastp_kegg lcl|cit:102625411 1 2255 + 2255 Gaps:34 100.00 2277 71.81 0.0 mediator of RNA polymerase II transcription subunit 12-like
blastp_kegg lcl|pxb:103932267 4 2255 + 2252 Gaps:45 99.96 2264 71.50 0.0 mediator of RNA polymerase II transcription subunit 12-like
blastp_kegg lcl|fve:101300384 1 2255 + 2255 Gaps:48 100.00 2261 71.65 0.0 mediator of RNA polymerase II transcription subunit 12-like
blastp_kegg lcl|rcu:RCOM_1615280 1 2255 + 2255 Gaps:51 100.00 2264 72.00 0.0 CRP putative
blastp_kegg lcl|pper:PRUPE_ppa000036mg 1 2255 + 2255 Gaps:99 100.00 2210 72.76 0.0 hypothetical protein
blastp_kegg lcl|mdm:103401906 1 2255 + 2255 Gaps:58 100.00 2257 71.73 0.0 mediator of RNA polymerase II transcription subunit 12
blastp_kegg lcl|pxb:103957424 1 2255 + 2255 Gaps:47 100.00 2268 70.68 0.0 mediator of RNA polymerase II transcription subunit 12-like
blastp_kegg lcl|cic:CICLE_v10018441mg 43 2255 + 2213 Gaps:34 99.82 2239 71.63 0.0 hypothetical protein
blastp_uniprot_sprot sp|H3K2Y6|MED12_ARATH 1 2255 + 2255 Gaps:74 100.00 2235 60.58 0.0 Mediator of RNA polymerase II transcription subunit 12 OS Arabidopsis thaliana GN MED12 PE 1 SV 1
blastp_uniprot_sprot sp|Q54U49|MED12_DICDI 44 369 + 326 Gaps:37 10.73 2731 29.01 1e-14 Putative mediator of RNA polymerase II transcription subunit 12 OS Dictyostelium discoideum GN med12 PE 3 SV 2
blastp_uniprot_sprot sp|Q6FVM9|SRB8_CANGA 67 263 + 197 Gaps:32 14.65 1358 27.64 1e-08 Mediator of RNA polymerase II transcription subunit 12 OS Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) GN SRB8 PE 3 SV 1
blastp_uniprot_sprot sp|Q751D2|SRB8_ASHGO 56 289 + 234 Gaps:33 17.60 1358 25.94 2e-07 Mediator of RNA polymerase II transcription subunit 12 OS Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) GN SRB8 PE 3 SV 2
blastp_uniprot_sprot sp|P25648|SRB8_YEAST 67 289 + 223 Gaps:33 15.14 1427 26.39 5e-07 Mediator of RNA polymerase II transcription subunit 12 OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN SRB8 PE 1 SV 2
blastp_uniprot_sprot sp|Q9VW47|MED12_DROME 56 268 + 213 Gaps:36 7.86 2531 28.64 6e-07 Mediator of RNA polymerase II transcription subunit 12 OS Drosophila melanogaster GN kto PE 1 SV 2
blastp_uniprot_sprot sp|Q7SGG0|SRB8_NEUCR 75 421 + 347 Gaps:77 16.32 1789 30.82 6e-06 Mediator of RNA polymerase II transcription subunit 12 OS Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) GN srb8 PE 3 SV 1
rpsblast_cdd gnl|CDD|192306 160 219 + 60 none 93.75 64 38.33 3e-15 pfam09497 Med12 Transcription mediator complex subunit Med12. Med12 is a negative regulator of the Gli3-dependent sonic hedgehog signalling pathway via its interaction with Gli3 within the RNA polymerase II transcriptional Mediator. A complex is formed between Med12 Med13 CDK8 and CycC which is responsible for suppression of transcription. This subunit forms part of the Kinase section of Mediator.
rpsblast_kog gnl|CDD|39722 79 2248 + 2170 Gaps:471 100.00 1427 49.68 1e-180 KOG4522 KOG4522 KOG4522 RNA polymerase II transcription mediator [Transcription].

18 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
PANTHER 1297 1445 149 PTHR22795 none none none
PANTHER 7 529 523 PTHR22795 none none none
Phobius 1532 1551 20 TRANSMEMBRANE none Region of a membrane-bound protein predicted to be embedded in the membrane. none
PANTHER 550 594 45 PTHR22795:SF18 none none none
PANTHER 640 713 74 PTHR22795:SF18 none none none
PANTHER 1297 1445 149 PTHR22795:SF18 none none none
PANTHER 7 529 523 PTHR22795:SF18 none none none
PANTHER 834 1197 364 PTHR22795:SF18 none none none
Phobius 1552 2255 704 NON_CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. none
PANTHER 640 713 74 PTHR22795 none none none
PANTHER 1484 2203 720 PTHR22795 none none none
Phobius 1431 1450 20 TRANSMEMBRANE none Region of a membrane-bound protein predicted to be embedded in the membrane. none
Phobius 1 1430 1430 NON_CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. none
Pfam 160 219 60 PF09497 none Transcription mediator complex subunit Med12 IPR019035
PANTHER 550 594 45 PTHR22795 none none none
PANTHER 834 1197 364 PTHR22795 none none none
Phobius 1451 1531 81 CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. none
PANTHER 1484 2203 720 PTHR22795:SF18 none none none

0 Localization

11 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Bourran2_2014_nSecLBD_3P Qrob_Chr08 8 s_1BN2OD_551 s_1B5AYF_599 17,17 0 43,51 lod 1,9229 4,4
Bourran2_2015_nEpiBC_3P Qrob_Chr12 12 s_1B73S5_217 v_7050_211 28,31 26,37 28,45 lod 4.5 11.6
Bourran2_2014_aSeqBC_A4 Qrob_Chr08 8 v_15999_278 v_AP13YL15_395 32,52 4,22 57,22 lod 2,7561 6,7
Bourran2_2014_nFork*_A4 Qrob_Chr08 8 PIE175 s_1CD7GJ_1398 31,22 5,24 57,24 lod 2,6724 6,8
Bourran2_2014_nLBD*_3P Qrob_Chr08 8 v_5216_549 v_11837_70 12,25 0 35,55 lod 2,5951 6
Bourran2_2014_nP*_3P Qrob_Chr08 8 v_5216_549 v_11837_70 12,19 0 31,97 lod 2,8472 6
Bourran2_2014_nPriLBD_3P Qrob_Chr08 8 v_5216_549 v_11837_70 12,36 0 30,43 lod 2,5806 5,1
Bourran2_2015_rEpiBC_3P Qrob_Chr08 8 s_A9TNV_543 v_11837_70 9,93 9,83 11,15 lod 3.3 7.3
Champenoux_2015_nSeqBC_A4 Qrob_Chr08 8 v_AD7YD13_501 s_1A7IED_780 43,44 43,42 43,99 lod 3.7 8.9
Bourran2_2014_nEpis*_3P Qrob_Chr08 8 s_1DA4QW_688 s_1DNI7D_820 17,96 0 37,75 lod 2,9745 7,5
Bourran2_2014_nPriBD_3P Qrob_Chr11 11 v_11486_194 s_1AT3E_2335 5,54 0,4 20,6 lod 2,6345 5,9

1 Targeting

Analysis Start End Length Location Reliability Signal Peptide Cut Off Mitochondrion Cut Off Network Signal Peptide Length
TargetP 1 53   Mitochondrion 4 0.026 0.693 NON-PLANT 53