Protein : Qrob_P0641350.2 Q. robur
Protein Identifier  ? Qrob_P0641350.2 Organism . Name  Quercus robur
Score  100.0 Score Type  egn
Protein Description  (M=2) K01870 - isoleucyl-tRNA synthetase [EC:6.1.1.5] Code Enzyme  EC:6.1.1.5
Gene Prediction Quality  validated Protein length 

Sequence

Length: 745  
Kegg Orthology  K01870
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0 Synonyms

9 GO Terms

Identifier Name Description
GO:0005524 ATP binding Interacting selectively and non-covalently with ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
GO:0005737 cytoplasm All of the contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
GO:0003824 catalytic activity Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic.
GO:0000166 nucleotide binding Interacting selectively and non-covalently with a nucleotide, any compound consisting of a nucleoside that is esterified with (ortho)phosphate or an oligophosphate at any hydroxyl group on the ribose or deoxyribose.
GO:0004812 aminoacyl-tRNA ligase activity Catalysis of the formation of aminoacyl-tRNA from ATP, amino acid, and tRNA with the release of diphosphate and AMP.
GO:0006418 tRNA aminoacylation for protein translation The synthesis of aminoacyl tRNA by the formation of an ester bond between the 3'-hydroxyl group of the most 3' adenosine of the tRNA, to be used in ribosome-mediated polypeptide synthesis.
GO:0002161 aminoacyl-tRNA editing activity The hydrolysis of an incorrectly aminoacylated tRNA.
GO:0004822 isoleucine-tRNA ligase activity Catalysis of the reaction: L-isoleucine + ATP + tRNA(Ile) = L-isoleucyl-tRNA(Ile) + AMP + diphosphate + 2 H(+).
GO:0006428 isoleucyl-tRNA aminoacylation The process of coupling isoleucine to isoleucyl-tRNA, catalyzed by isoleucyl-tRNA synthetase. In tRNA aminoacylation, the amino acid is first activated by linkage to AMP and then transferred to either the 2'- or the 3'-hydroxyl group of the 3'-adenosine residue of the tRNA.

44 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|pper:PRUPE_ppa000564mg 8 744 + 737 Gaps:2 67.12 1095 84.76 0.0 hypothetical protein
blastp_kegg lcl|pmum:103323008 8 744 + 737 Gaps:2 67.31 1092 84.63 0.0 isoleucine--tRNA ligase mitochondrial
blastp_kegg lcl|pop:POPTR_0010s01830g 8 741 + 734 none 74.82 981 83.24 0.0 POPTRDRAFT_1089196 tRNA synthetase class I family protein
blastp_kegg lcl|vvi:100254604 8 744 + 737 Gaps:1 66.61 1105 83.97 0.0 isoleucyl-tRNA synthetase-like
blastp_kegg lcl|pxb:103943653 8 744 + 737 Gaps:5 67.34 1093 83.83 0.0 isoleucine--tRNA ligase mitochondrial
blastp_kegg lcl|cam:101515155 3 735 + 733 none 67.87 1080 81.72 0.0 isoleucine--tRNA ligase-like
blastp_kegg lcl|tcc:TCM_009231 8 744 + 737 Gaps:2 67.25 1093 82.31 0.0 TRNA synthetase class I (I L M and V) family protein isoform 1
blastp_kegg lcl|rcu:RCOM_0681070 3 744 + 742 Gaps:1 67.24 1102 80.84 0.0 isoleucyl tRNA synthetase putative (EC:6.1.1.5)
blastp_kegg lcl|mdm:103452907 8 744 + 737 Gaps:5 67.34 1093 83.02 0.0 isoleucine--tRNA ligase mitochondrial-like
blastp_kegg lcl|pvu:PHAVU_003G105600g 3 744 + 742 Gaps:1 67.92 1094 80.89 0.0 hypothetical protein
blastp_pdb 1qu3_A 66 732 + 667 Gaps:45 68.70 917 46.03 2e-179 mol:protein length:917 ISOLEUCYL-TRNA SYNTHETASE
blastp_pdb 1qu2_A 66 732 + 667 Gaps:45 68.70 917 46.03 2e-179 mol:protein length:917 ISOLEUCYL-TRNA SYNTHETASE
blastp_pdb 1ffy_A 66 732 + 667 Gaps:45 68.70 917 46.03 2e-179 mol:protein length:917 ISOLEUCYL-TRNA SYNTHETASE
blastp_pdb 1jzs_A 56 561 + 506 Gaps:48 62.12 821 32.75 3e-60 mol:protein length:821 Isoleucyl-tRNA synthetase
blastp_pdb 1jzq_A 56 561 + 506 Gaps:48 62.12 821 32.75 3e-60 mol:protein length:821 Isoleucyl-tRNA synthetase
blastp_pdb 1ile_A 56 561 + 506 Gaps:48 62.12 821 32.75 3e-60 mol:protein length:821 ISOLEUCYL-TRNA SYNTHETASE
blastp_pdb 1iyw_B 76 561 + 486 Gaps:72 53.83 862 26.72 3e-28 mol:protein length:862 Valyl-tRNA Synthetase
blastp_pdb 1iyw_A 76 561 + 486 Gaps:72 53.83 862 26.72 3e-28 mol:protein length:862 Valyl-tRNA Synthetase
blastp_pdb 1ivs_B 76 561 + 486 Gaps:72 53.83 862 26.72 3e-28 mol:protein length:862 Valyl-tRNA synthetase
blastp_pdb 1ivs_A 76 561 + 486 Gaps:72 53.83 862 26.72 3e-28 mol:protein length:862 Valyl-tRNA synthetase
blastp_uniprot_sprot sp|Q8DGI7|SYI_THEEB 1 728 + 728 Gaps:51 72.51 953 58.47 0.0 Isoleucine--tRNA ligase OS Thermosynechococcus elongatus (strain BP-1) GN ileS PE 3 SV 2
blastp_uniprot_sprot sp|B7K5H5|SYI_CYAP8 1 728 + 728 Gaps:40 72.78 959 58.74 0.0 Isoleucine--tRNA ligase OS Cyanothece sp. (strain PCC 8801) GN ileS PE 3 SV 1
blastp_uniprot_sprot sp|B1WVA3|SYI_CYAA5 1 728 + 728 Gaps:44 72.78 959 57.74 0.0 Isoleucine--tRNA ligase OS Cyanothece sp. (strain ATCC 51142) GN ileS PE 3 SV 1
blastp_uniprot_sprot sp|B2IZP5|SYI_NOSP7 1 728 + 728 Gaps:46 72.78 959 57.74 0.0 Isoleucine--tRNA ligase OS Nostoc punctiforme (strain ATCC 29133 / PCC 73102) GN ileS PE 3 SV 1
blastp_uniprot_sprot sp|Q8YXY3|SYI_NOSS1 1 728 + 728 Gaps:35 72.81 960 56.65 0.0 Isoleucine--tRNA ligase OS Nostoc sp. (strain PCC 7120 / UTEX 2576) GN ileS PE 3 SV 1
blastp_uniprot_sprot sp|B8HMX3|SYI_CYAP4 40 733 + 694 Gaps:21 71.94 955 56.91 0.0 Isoleucine--tRNA ligase OS Cyanothece sp. (strain PCC 7425 / ATCC 29141) GN ileS PE 3 SV 1
blastp_uniprot_sprot sp|P73505|SYI_SYNY3 1 728 + 728 Gaps:55 73.58 988 55.16 0.0 Isoleucine--tRNA ligase OS Synechocystis sp. (strain PCC 6803 / Kazusa) GN ileS PE 3 SV 1
blastp_uniprot_sprot sp|B7KFT1|SYI_CYAP7 1 728 + 728 Gaps:40 72.78 959 55.87 0.0 Isoleucine--tRNA ligase OS Cyanothece sp. (strain PCC 7424) GN ileS PE 3 SV 1
blastp_uniprot_sprot sp|B0JSP4|SYI_MICAN 1 728 + 728 Gaps:48 72.90 952 56.05 0.0 Isoleucine--tRNA ligase OS Microcystis aeruginosa (strain NIES-843) GN ileS PE 3 SV 1
blastp_uniprot_sprot sp|B0C872|SYI_ACAM1 45 733 + 689 Gaps:21 69.82 971 57.23 0.0 Isoleucine--tRNA ligase OS Acaryochloris marina (strain MBIC 11017) GN ileS PE 3 SV 1

16 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
SUPERFAMILY 4 178 175 SSF50677 "Reactome:REACT_71" none IPR009008
PANTHER 3 27 25 PTHR11946:SF9 "KEGG:00970+6.1.1.5";signature_desc=ISOLEUCYL TRNA SYNTHETASE none IPR023585
PANTHER 71 732 662 PTHR11946:SF9 "KEGG:00970+6.1.1.5";signature_desc=ISOLEUCYL TRNA SYNTHETASE none IPR023585
PANTHER 3 27 25 PTHR11946 none none none
Pfam 42 421 380 PF00133 "Reactome:REACT_71" tRNA synthetases class I (I, L, M and V) IPR002300
Pfam 468 632 165 PF08264 "Reactome:REACT_71" Anticodon-binding domain of tRNA IPR013155
SUPERFAMILY 436 733 298 SSF47323 "Reactome:REACT_71" none IPR009080
PANTHER 71 732 662 PTHR11946 none none none
Gene3D 3 184 182 G3DSA:3.90.740.10 "Reactome:REACT_71" none IPR009008
SUPERFAMILY 178 432 255 SSF52374 none none none
Gene3D 429 616 188 G3DSA:1.10.730.10 "Reactome:REACT_71" none IPR009080
Gene3D 185 421 237 G3DSA:3.40.50.620 none none IPR014729
PRINTS 336 345 10 PR00984 "KEGG:00970+6.1.1.5","Reactome:REACT_71" Isoleucyl-tRNA synthetase signature IPR002301
PRINTS 176 191 16 PR00984 "KEGG:00970+6.1.1.5","Reactome:REACT_71" Isoleucyl-tRNA synthetase signature IPR002301
PRINTS 302 315 14 PR00984 "KEGG:00970+6.1.1.5","Reactome:REACT_71" Isoleucyl-tRNA synthetase signature IPR002301
Pfam 702 731 30 PF06827 none Zinc finger found in FPG and IleRS IPR010663

0 Localization

17 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Bourran2_2014_nSecLBD_3P Qrob_Chr08 8 s_1BN2OD_551 s_1B5AYF_599 17,17 0 43,51 lod 1,9229 4,4
Bourran2_2014_nLBD*_3P Qrob_Chr08 8 v_5216_549 v_11837_70 12,25 0 35,55 lod 2,5951 6
Bourran2_2014_nP*_3P Qrob_Chr08 8 v_5216_549 v_11837_70 12,19 0 31,97 lod 2,8472 6
Bourran2_2002_QTL11_peak_Bud_burst_A4 Qrob_Chr06 6 s_1C41PA_791 s_1AM1AV_1141 19,17 0 34,57 lod 2,3 2,9
Bourran2_2014_aSeqBC_3P Qrob_Chr06 6 v_506_189 v_686_77 30,72 13,58 43,48 lod 2,2746 6,3
Bourran2_2014_aSeqBC*_A4 Qrob_Chr06 6 s_2F5MK3_712 v_444_355 27,13 14,86 39,46 lod 3,7847 9,8
Bourran2_2014_nEpis*_3P Qrob_Chr08 8 s_1DA4QW_688 s_1DNI7D_820 17,96 0 37,75 lod 2,9745 7,5
Bourran2_2014_nEpis*_A4 Qrob_Chr07 7 v_12400_446 s_1BPEBU_1211 6,93 0 15,13 lod 4,7411 11
Bourran2_2014_nFork*_3P Qrob_Chr06 6 v_12930_125 s_1AMZEI_909 34,28 7,43 41,48 lod 2,4044 5,5
Bourran2_2014_nLBD_A4 Qrob_Chr06 6 v_12930_125 s_1AMZEI_909 37,41 9,8 50,1 lod 1,9524 4,1
Bourran2_2014_nPriBD_A4 Qrob_Chr06 6 v_12930_125 s_1AMZEI_909 34,51 3,36 51,86 lod 1,6747 3,9
Bourran2_2014_nPriLBD*_3P Qrob_Chr11 11 v_12066_307 s_1B15GJ_447 3,37 0,57 16,17 lod 3,4299 6,9
Bourran2_2014_nSecLBD_A4 Qrob_Chr07 7 v_8327_222 s_1A4WGY_363 16,04 0 44,69 lod 2,6373 6,5
Bourran2_2014_vEpiBC_A4 Qrob_Chr06 6 v_12930_125 s_1AMZEI_909 37,55 14,41 50,01 lod 1,7882 4,8
Champenoux_2015_nEpis_3P Qrob_Chr11 11 s_1DG9PM_867 s_1BZ083_1312 26,53 25,47 27,72 lod 4.4 8.9
Champenoux_2015_nP_3P Qrob_Chr06 6 s_1A386O_228 s_1AYZFS_603 27,03 26,47 27,34 lod 2.8 7.2
Bourran2_2014_nPriBD*_3P Qrob_Chr06 6 s_1B6WLL_980 v_10064_133 11,49 0 31,52 lod 4,1208 9,1

0 Targeting