Protein : Qrob_P0515690.2 Q. robur
Protein Identifier  ? Qrob_P0515690.2 Organism . Name  Quercus robur
Score  99.0 Score Type  egn
Protein Description  (M=1) K05543 - tRNA-dihydrouridine synthase 2 [EC:1.-.-.-] Code Enzyme  EC:1.3.1.91
Gene Prediction Quality  validated Protein length 

Sequence

Length: 180  
Kegg Orthology  K05543
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0 Synonyms

5 GO Terms

Identifier Name Description
GO:0055114 oxidation-reduction process A metabolic process that results in the removal or addition of one or more electrons to or from a substance, with or without the concomitant removal or addition of a proton or protons.
GO:0003824 catalytic activity Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic.
GO:0050660 flavin adenine dinucleotide binding Interacting selectively and non-covalently with FAD, flavin-adenine dinucleotide, the coenzyme or the prosthetic group of various flavoprotein oxidoreductase enzymes, in either the oxidized form, FAD, or the reduced form, FADH2.
GO:0008033 tRNA processing The process in which a pre-tRNA molecule is converted to a mature tRNA, ready for addition of an aminoacyl group.
GO:0017150 tRNA dihydrouridine synthase activity Catalysis of the reaction: tRNA-uracil + acceptor = tRNA-dihydrouridine + reduced acceptor.

38 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|rcu:RCOM_0188130 1 179 + 179 none 54.24 330 92.18 2e-117 tRNA-dihydrouridine synthase putative
blastp_kegg lcl|tcc:TCM_000905 1 179 + 179 none 54.91 326 89.94 2e-116 TRNA-dihydrouridine synthase
blastp_kegg lcl|cit:102628574 1 179 + 179 none 54.91 326 89.94 2e-115 tRNA-dihydrouridine(20) synthase [NAD(P)+]-like
blastp_kegg lcl|cic:CICLE_v10026068mg 1 179 + 179 none 54.91 326 89.39 7e-115 hypothetical protein
blastp_kegg lcl|pvu:PHAVU_003G285100g 1 179 + 179 none 55.42 323 87.71 3e-113 hypothetical protein
blastp_kegg lcl|mus:103971623 1 179 + 179 none 55.94 320 88.27 7e-112 tRNA-dihydrouridine(20) synthase [NAD(P)+]-like
blastp_kegg lcl|pmum:103336703 1 179 + 179 none 56.29 318 86.59 2e-111 tRNA-dihydrouridine(20) synthase [NAD(P)+]-like
blastp_kegg lcl|pper:PRUPE_ppa008825mg 1 179 + 179 none 56.29 318 86.03 1e-110 hypothetical protein
blastp_kegg lcl|gmx:100787829 1 179 + 179 none 55.94 320 87.15 3e-110 tRNA-dihydrouridine(20) synthase [NAD(P)+]-like
blastp_kegg lcl|sly:101254211 1 179 + 179 none 54.91 326 84.92 2e-109 tRNA-dihydrouridine(20) synthase [NAD(P)+]-like
blastp_pdb 1vhn_A 6 179 + 174 Gaps:17 49.37 318 28.66 7e-10 mol:protein length:318 putative flavin oxidoreducatase
blastp_pdb 3b0u_Y 39 179 + 141 Gaps:16 43.14 350 28.48 5e-07 mol:protein length:350 tRNA-dihydrouridine synthase
blastp_pdb 3b0u_X 39 179 + 141 Gaps:16 43.14 350 28.48 5e-07 mol:protein length:350 tRNA-dihydrouridine synthase
blastp_pdb 3b0p_B 39 179 + 141 Gaps:16 43.14 350 28.48 5e-07 mol:protein length:350 tRNA-dihydrouridine synthase
blastp_pdb 3b0p_A 39 179 + 141 Gaps:16 43.14 350 28.48 5e-07 mol:protein length:350 tRNA-dihydrouridine synthase
blastp_pdb 3b0v_D 39 179 + 141 Gaps:16 41.60 363 28.48 6e-07 mol:protein length:363 tRNA-dihydrouridine synthase
blastp_pdb 3b0v_C 39 179 + 141 Gaps:16 41.60 363 28.48 6e-07 mol:protein length:363 tRNA-dihydrouridine synthase
blastp_uniprot_sprot sp|Q9NX74|DUS2L_HUMAN 3 179 + 177 Gaps:1 35.70 493 62.50 4e-72 tRNA-dihydrouridine(20) synthase [NAD(P)+]-like OS Homo sapiens GN DUS2 PE 1 SV 1
blastp_uniprot_sprot sp|Q9D7B1|DUS2L_MOUSE 3 179 + 177 Gaps:1 35.70 493 62.50 2e-71 tRNA-dihydrouridine(20) synthase [NAD(P)+]-like OS Mus musculus GN Dus2 PE 1 SV 1
blastp_uniprot_sprot sp|O74731|DUS2_SCHPO 1 179 + 179 Gaps:7 38.83 479 44.62 9e-47 tRNA-dihydrouridine(20) synthase [NAD(P)+] OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN dus2 PE 3 SV 1
blastp_uniprot_sprot sp|P53720|DUS2_YEAST 3 179 + 177 Gaps:10 48.70 384 44.39 2e-44 tRNA-dihydrouridine(20) synthase [NAD(P)+] OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN SMM1 PE 1 SV 1
blastp_uniprot_sprot sp|Q9HGN6|DUS1_SCHPO 2 179 + 178 Gaps:23 42.36 399 40.24 2e-24 tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)] OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN dus1 PE 3 SV 1
blastp_uniprot_sprot sp|Q32M08|DUS4L_MOUSE 8 179 + 172 Gaps:19 49.07 324 37.11 3e-24 tRNA-dihydrouridine(20a/20b) synthase [NAD(P)+]-like OS Mus musculus GN Dus4l PE 2 SV 1
blastp_uniprot_sprot sp|O95620|DUS4L_HUMAN 8 179 + 172 Gaps:19 50.16 317 37.11 3e-24 tRNA-dihydrouridine(20a/20b) synthase [NAD(P)+]-like OS Homo sapiens GN DUS4L PE 2 SV 2
blastp_uniprot_sprot sp|O74553|DUS4_SCHPO 8 179 + 172 Gaps:19 48.16 326 38.22 2e-22 tRNA-dihydrouridine(20a/20b) synthase [NAD(P)+] OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN dus4 PE 3 SV 1
blastp_uniprot_sprot sp|P53759|DUS1_YEAST 8 179 + 172 Gaps:13 37.59 423 33.33 3e-19 tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)] OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN DUS1 PE 1 SV 1
blastp_uniprot_sprot sp|Q09504|YQI2_CAEEL 12 179 + 168 Gaps:21 54.58 284 36.77 7e-18 Uncharacterized tRNA-dihydrouridine synthase-like protein C45G9.2 OS Caenorhabditis elegans GN C45G9.2 PE 3 SV 2
rpsblast_cdd gnl|CDD|73368 6 179 + 174 Gaps:18 69.26 231 43.12 1e-44 cd02801 DUS_like_FMN Dihydrouridine synthase-like (DUS-like) FMN-binding domain. Members of this family catalyze the reduction of the 5 6-double bond of a uridine residue on tRNA. Dihydrouridine modification of tRNA is widely observed in prokaryotes and eukaryotes and also in some archaea. Most dihydrouridines are found in the D loop of t-RNAs. The role of dihydrouridine in tRNA is currently unknown but may increase conformational flexibility of the tRNA. It is likely that different family members have different substrate specificities which may overlap. 1VHN a putative flavin oxidoreductase has high sequence similarity to DUS. The enzymatic mechanism of 1VHN is not known at the present..
rpsblast_cdd gnl|CDD|30391 1 179 + 179 Gaps:21 52.01 323 37.50 1e-30 COG0042 COG0042 tRNA-dihydrouridine synthase [Translation ribosomal structure and biogenesis].
rpsblast_cdd gnl|CDD|144707 8 179 + 172 Gaps:22 51.78 309 38.75 1e-30 pfam01207 Dus Dihydrouridine synthase (Dus). Members of this family catalyze the reduction of the 5 6-double bond of a uridine residue on tRNA. Dihydrouridine modification of tRNA is widely observed in prokaryotes and eukaryotes and also in some archae. Most dihydrouridines are found in the D loop of t-RNAs. The role of dihydrouridine in tRNA is currently unknown but may increase conformational flexibility of the tRNA. It is likely that different family members have different substrate specificities which may overlap. Dus 1 from Saccharomyces cerevisiae acts on pre-tRNA-Phe while Dus 2 acts on pre-tRNA-Tyr and pre-tRNA-Leu. Dus 1 is active as a single subunit requiring NADPH or NADH and is stimulated by the presence of FAD. Some family members may be targeted to the mitochondria and even have a role in mitochondria.

6 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
PANTHER 1 179 179 PTHR11082 none none IPR001269
Gene3D 2 179 178 G3DSA:3.20.20.70 none none IPR013785
SUPERFAMILY 5 179 175 SSF51395 none none none
Pfam 8 179 172 PF01207 none Dihydrouridine synthase (Dus) IPR001269
PANTHER 1 179 179 PTHR11082:SF4 none none none
ProSitePatterns 104 122 19 PS01136 none Uncharacterized protein family UPF0034 signature. IPR018517

0 Localization

7 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Bourran2_2014_nEpis*_3P Qrob_Chr08 8 s_1DA4QW_688 s_1DNI7D_820 17,96 0 37,75 lod 2,9745 7,5
Bourran2_2014_nEpis*_A4 Qrob_Chr07 7 v_12400_446 s_1BPEBU_1211 6,93 0 15,13 lod 4,7411 11
Bourran2_2014_nLBD_A4 Qrob_Chr06 6 v_12930_125 s_1AMZEI_909 37,41 9,8 50,1 lod 1,9524 4,1
Bourran2_2014_nPriBD_A4 Qrob_Chr06 6 v_12930_125 s_1AMZEI_909 34,51 3,36 51,86 lod 1,6747 3,9
Bourran2_2014_nSecLBD_A4 Qrob_Chr07 7 v_8327_222 s_1A4WGY_363 16,04 0 44,69 lod 2,6373 6,5
Bourran2_2014_vEpiBC_A4 Qrob_Chr06 6 v_12930_125 s_1AMZEI_909 37,55 14,41 50,01 lod 1,7882 4,8
Champenoux_2015_nEpis_3P Qrob_Chr11 11 s_1DG9PM_867 s_1BZ083_1312 26,53 25,47 27,72 lod 4.4 8.9

0 Targeting