Protein : Qrob_P0446560.2 Q. robur
Protein Identifier  ? Qrob_P0446560.2 Organism . Name  Quercus robur
Score  99.0 Score Type  egn
Protein Description  (M=1) 1.3.1.33 - Protochlorophyllide reductase. Code Enzyme  EC:1.3.1.33
Gene Prediction Quality  validated Protein length 

Sequence

Length: 401  
Kegg Orthology  K00218
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0 Synonyms

4 GO Terms

Identifier Name Description
GO:0008152 metabolic process The chemical reactions and pathways, including anabolism and catabolism, by which living organisms transform chemical substances. Metabolic processes typically transform small molecules, but also include macromolecular processes such as DNA repair and replication, and protein synthesis and degradation.
GO:0016491 oxidoreductase activity Catalysis of an oxidation-reduction (redox) reaction, a reversible chemical reaction in which the oxidation state of an atom or atoms within a molecule is altered. One substrate acts as a hydrogen or electron donor and becomes oxidized, while the other acts as hydrogen or electron acceptor and becomes reduced.
GO:0055114 oxidation-reduction process A metabolic process that results in the removal or addition of one or more electrons to or from a substance, with or without the concomitant removal or addition of a proton or protons.
GO:0016630 protochlorophyllide reductase activity Catalysis of the reaction: chlorophyllide a + NADP+ = protochlorophyllide + NADPH + H+.

45 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|vvi:100241491 1 400 + 400 Gaps:3 100.00 397 87.91 0.0 protochlorophyllide reductase chloroplastic-like
blastp_kegg lcl|tcc:TCM_008067 1 400 + 400 none 91.53 437 82.25 0.0 Protochlorophyllide oxidoreductase C C PORC
blastp_kegg lcl|pop:POPTR_0013s13910g 1 400 + 400 Gaps:1 100.00 401 83.04 0.0 POPTRDRAFT_571754 Protochlorophyllide reductase C family protein
blastp_kegg lcl|cmo:103493499 1 400 + 400 Gaps:5 100.00 399 83.46 0.0 protochlorophyllide reductase chloroplastic
blastp_kegg lcl|rcu:RCOM_1175550 1 400 + 400 Gaps:2 100.00 402 82.84 0.0 short-chain dehydrogenase putative (EC:1.3.1.33)
blastp_kegg lcl|vvi:100255647 1 400 + 400 Gaps:4 100.00 396 83.33 0.0 protochlorophyllide reductase chloroplastic-like
blastp_kegg lcl|pxb:103966968 1 400 + 400 Gaps:7 100.00 395 81.52 0.0 protochlorophyllide reductase chloroplastic
blastp_kegg lcl|pxb:103935530 1 400 + 400 Gaps:7 100.00 395 81.77 0.0 protochlorophyllide reductase chloroplastic
blastp_kegg lcl|sly:101248079 1 400 + 400 Gaps:3 100.00 397 80.35 0.0 protochlorophyllide reductase chloroplastic-like
blastp_kegg lcl|cic:CICLE_v10008520mg 1 400 + 400 Gaps:5 100.00 399 82.71 0.0 hypothetical protein
blastp_pdb 3rd5_A 89 292 + 204 Gaps:39 56.70 291 45.45 3e-20 mol:protein length:291 MYPAA.01249.C
blastp_pdb 3rku_D 80 230 + 151 Gaps:6 53.31 287 28.76 1e-06 mol:protein length:287 Oxidoreductase YMR226C
blastp_pdb 3rku_C 80 230 + 151 Gaps:6 53.31 287 28.76 1e-06 mol:protein length:287 Oxidoreductase YMR226C
blastp_pdb 3rku_B 80 230 + 151 Gaps:6 53.31 287 28.76 1e-06 mol:protein length:287 Oxidoreductase YMR226C
blastp_pdb 3rku_A 80 230 + 151 Gaps:6 53.31 287 28.76 1e-06 mol:protein length:287 Oxidoreductase YMR226C
blastp_pdb 1vl8_B 85 229 + 145 Gaps:11 53.18 267 29.58 8e-06 mol:protein length:267 Gluconate 5-dehydrogenase
blastp_pdb 1vl8_A 85 229 + 145 Gaps:11 53.18 267 29.58 8e-06 mol:protein length:267 Gluconate 5-dehydrogenase
blastp_uniprot_sprot sp|Q41249|PORA_CUCSA 1 400 + 400 Gaps:6 100.00 398 81.91 0.0 Protochlorophyllide reductase chloroplastic OS Cucumis sativus GN PORA PE 2 SV 1
blastp_uniprot_sprot sp|Q9SDT1|POR_DAUCA 1 400 + 400 Gaps:4 100.00 398 79.90 0.0 Protochlorophyllide reductase chloroplastic OS Daucus carota GN POR1 PE 2 SV 1
blastp_uniprot_sprot sp|O48741|PORC_ARATH 1 400 + 400 Gaps:3 100.00 401 79.55 0.0 Protochlorophyllide reductase C chloroplastic OS Arabidopsis thaliana GN PORC PE 1 SV 1
blastp_uniprot_sprot sp|Q42536|PORA_ARATH 1 400 + 400 Gaps:15 100.00 405 76.54 0.0 Protochlorophyllide reductase A chloroplastic OS Arabidopsis thaliana GN PORA PE 1 SV 2
blastp_uniprot_sprot sp|Q01289|POR_PEA 1 400 + 400 Gaps:5 100.00 399 78.70 0.0 Protochlorophyllide reductase chloroplastic OS Pisum sativum GN 3PCR PE 1 SV 1
blastp_uniprot_sprot sp|P21218|PORB_ARATH 1 400 + 400 Gaps:9 100.00 401 77.81 0.0 Protochlorophyllide reductase B chloroplastic OS Arabidopsis thaliana GN PORB PE 1 SV 3
blastp_uniprot_sprot sp|Q42850|PORB_HORVU 1 400 + 400 Gaps:9 100.00 395 75.70 0.0 Protochlorophyllide reductase B chloroplastic OS Hordeum vulgare GN PORB PE 2 SV 1
blastp_uniprot_sprot sp|Q8W3D9|PORB_ORYSJ 1 400 + 400 Gaps:12 99.00 402 74.12 0.0 Protochlorophyllide reductase B chloroplastic OS Oryza sativa subsp. japonica GN PORB PE 2 SV 1
blastp_uniprot_sprot sp|O80333|POR_MARPA 2 399 + 398 Gaps:2 86.46 458 66.92 0.0 Protochlorophyllide reductase chloroplastic OS Marchantia paleacea GN PORA PE 2 SV 1
blastp_uniprot_sprot sp|Q7XKF3|PORA_ORYSJ 1 399 + 399 Gaps:19 99.22 387 72.92 0.0 Protochlorophyllide reductase A chloroplastic OS Oryza sativa subsp. japonica GN PORA PE 2 SV 1
rpsblast_cdd gnl|CDD|177654 91 398 + 308 none 100.00 308 90.58 0.0 PLN00015 PLN00015 protochlorophyllide reductase.
rpsblast_cdd gnl|CDD|200089 85 400 + 316 Gaps:2 100.00 314 77.07 1e-165 TIGR01289 LPOR light-dependent protochlorophyllide reductase. This model represents the light-dependent NADPH-dependent form of protochlorophyllide reductase. It belongs to the short chain alcohol dehydrogenase family in contrast to the nitrogenase-related light-independent form.
rpsblast_cdd gnl|CDD|187670 87 399 + 313 Gaps:2 100.00 311 76.53 1e-157 cd09810 LPOR_like_SDR_c_like light-dependent protochlorophyllide reductase (LPOR)-like classical (c)-like SDRs. Classical SDR-like subgroup containing LPOR and related proteins. Protochlorophyllide (Pchlide) reductases act in chlorophyll biosynthesis. There are distinct enzymes that catalyze Pchlide reduction in light or dark conditions. Light-dependent reduction is via an NADP-dependent SDR LPOR. Proteins in this subfamily share the glycine-rich NAD-binding motif of the classical SDRs have a partial match to the canonical active site tetrad but lack the typical active site Ser. SDRs are a functionally diverse family of oxidoreductases that have a single domain with a structurally conserved Rossmann fold (alpha/beta folding pattern with a central beta-sheet) an NAD(P)(H)-binding region and a structurally diverse C-terminal region. Classical SDRs are typically about 250 residues long while extended SDRs are approximately 350 residues. Sequence identity between different SDR enzymes are typically in the 15-30% range but the enzymes share the Rossmann fold NAD-binding motif and characteristic NAD-binding and catalytic sequence patterns. These enzymes catalyze a wide range of activities including the metabolism of steroids cofactors carbohydrates lipids aromatic compounds and amino acids and act in redox sensing. Classical SDRs have an TGXXX[AG]XG cofactor binding motif and a YXXXK active site motif with the Tyr residue of the active site motif serving as a critical catalytic residue (Tyr-151 human 15-hydroxyprostaglandin dehydrogenase (15-PGDH) numbering). In addition to the Tyr and Lys there is often an upstream Ser (Ser-138 15-PGDH numbering) and/or an Asn (Asn-107 15-PGDH numbering) contributing to the active site while substrate binding is in the C-terminal region which determines specificity. The standard reaction mechanism is a 4-pro-S hydride transfer and proton relay involving the conserved Tyr and Lys a water molecule stabilized by Asn and nicotinamide. Extended SDRs have additional elements in the C-terminal region and typically have a TGXXGXXG cofactor binding motif. Complex (multidomain) SDRs such as ketoreductase domains of fatty acid synthase have a GGXGXXG NAD(P)-binding motif and an altered active site motif (YXXXN). Fungal type ketoacyl reductases have a TGXXXGX(1-2)G NAD(P)-binding motif. Some atypical SDRs have lost catalytic activity and/or have an unusual NAD(P)-binding motif and missing or unusual active site residues. Reactions catalyzed within the SDR family include isomerization decarboxylation epimerization C N bond reduction dehydratase activity dehalogenation Enoyl-CoA reduction and carbonyl-alcohol oxidoreduction.

18 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
SUPERFAMILY 84 233 150 SSF51735 none none none
SUPERFAMILY 269 368 100 SSF51735 none none none
TIGRFAM 86 400 315 TIGR01289 "KEGG:00860+1.3.1.33","UniPathway:UPA00668" LPOR: light-dependent protochlorophyllide reductase IPR005979
PRINTS 297 314 18 PR00081 none Glucose/ribitol dehydrogenase family signature IPR002347
PRINTS 164 175 12 PR00081 none Glucose/ribitol dehydrogenase family signature IPR002347
PRINTS 89 106 18 PR00081 none Glucose/ribitol dehydrogenase family signature IPR002347
Phobius 10 14 5 SIGNAL_PEPTIDE_C_REGION none C-terminal region of a signal peptide. none
Phobius 1 14 14 SIGNAL_PEPTIDE none Signal peptide region none
Pfam 89 230 142 PF00106 none short chain dehydrogenase IPR002198
PANTHER 40 246 207 PTHR24316:SF264 none none none
PANTHER 274 400 127 PTHR24316:SF264 none none none
PANTHER 40 246 207 PTHR24316 none none none
PANTHER 274 400 127 PTHR24316 none none none
Phobius 15 400 386 NON_CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. none
Phobius 1 1 1 SIGNAL_PEPTIDE_N_REGION none N-terminal region of a signal peptide. none
Phobius 2 9 8 SIGNAL_PEPTIDE_H_REGION none Hydrophobic region of a signal peptide. none
Gene3D 82 233 152 G3DSA:3.40.50.720 none none IPR016040
Gene3D 269 366 98 G3DSA:3.40.50.720 none none IPR016040

0 Localization

16 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Bourran2_2014_nLBD*_A4 Qrob_Chr08 8 v_12498_318 v_12364_308 34,91 16,12 53,62 lod 2,4961 5,2
Bourran2_2014_nSecLBD_3P Qrob_Chr08 8 s_1BN2OD_551 s_1B5AYF_599 17,17 0 43,51 lod 1,9229 4,4
Bourran2_2014_rEpiBC*_A4 Qrob_Chr08 8 v_12498_318 v_12364_308 35,77 14,11 55,31 lod 2,9413 6,2
Bourran2_2015_nEpiBC_3P Qrob_Chr12 12 s_1B73S5_217 v_7050_211 28,31 26,37 28,45 lod 4.5 11.6
Bourran_2000_2002_QTL3_Delta.F Qrob_Chr08 8 s_1A3EF7_1406 s_1AIWYC_607 30.17 21,01 40,21 lod 6.8553 0.055
Bourran2_2014_aSeqBC_A4 Qrob_Chr08 8 v_15999_278 v_AP13YL15_395 32,52 4,22 57,22 lod 2,7561 6,7
Bourran2_2014_nFork*_A4 Qrob_Chr08 8 PIE175 s_1CD7GJ_1398 31,22 5,24 57,24 lod 2,6724 6,8
Bourran2_2014_nLBD*_3P Qrob_Chr08 8 v_5216_549 v_11837_70 12,25 0 35,55 lod 2,5951 6
Bourran2_2014_nP*_3P Qrob_Chr08 8 v_5216_549 v_11837_70 12,19 0 31,97 lod 2,8472 6
Bourran2_2014_nPriLBD_3P Qrob_Chr08 8 v_5216_549 v_11837_70 12,36 0 30,43 lod 2,5806 5,1
Bourran2_2014_nPriLBD_A4 Qrob_Chr08 8 PIE175 v_9164_159 31,85 15,39 48,29 lod 2,8308 6,8
Bourran2_2015_rEpiBC_3P Qrob_Chr08 8 s_A9TNV_543 v_11837_70 9,93 9,83 11,15 lod 3.3 7.3
Champenoux_2015_nSeqBC_A4 Qrob_Chr08 8 v_AD7YD13_501 s_1A7IED_780 43,44 43,42 43,99 lod 3.7 8.9
NancyGreenhouseCO2_2001_ambient_elevated_leaf_cellulose_QTL3_d13Cf Qrob_Chr08 8 v_5216_549 v_11625_20 37.08 12,26 54,9 lod 6.5888 0.04
Bourran2_2014_nEpis*_3P Qrob_Chr08 8 s_1DA4QW_688 s_1DNI7D_820 17,96 0 37,75 lod 2,9745 7,5
Bourran2_2014_nPriBD_3P Qrob_Chr11 11 v_11486_194 s_1AT3E_2335 5,54 0,4 20,6 lod 2,6345 5,9

0 Targeting