Protein : Qrob_P0430900.2 Q. robur
Protein Identifier  ? Qrob_P0430900.2 Organism . Name  Quercus robur
Score  17.0 Score Type  egn
Protein Description  (M=4) PTHR11390:SF23 - DNA TOPOISOMERASE I-RELATED Code Enzyme  EC:5.99.1.2
Gene Prediction Quality  validated Protein length 

Sequence

Length: 169  
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0 Synonyms

4 GO Terms

Identifier Name Description
GO:0003677 DNA binding Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid).
GO:0006265 DNA topological change The process in which a transformation is induced in the topological structure of a double-stranded DNA helix, resulting in a change in linking number.
GO:0003917 DNA topoisomerase type I activity Catalysis of a DNA topological transformation by transiently cleaving one DNA strand at a time to allow passage of another strand; changes the linking number by +1 per catalytic cycle.
GO:0003916 DNA topoisomerase activity Catalysis of the transient cleavage and passage of individual DNA strands or double helices through one another, resulting a topological transformation in double-stranded DNA.

31 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|mdm:103431441 31 143 + 113 Gaps:23 25.28 538 72.06 1e-58 DNA topoisomerase 3-alpha-like
blastp_kegg lcl|pper:PRUPE_ppa020488mg 31 143 + 113 Gaps:23 25.09 542 71.32 2e-57 hypothetical protein
blastp_kegg lcl|pxb:103946776 31 143 + 113 Gaps:23 13.99 972 72.06 3e-57 DNA topoisomerase 3-alpha
blastp_kegg lcl|pmum:103327049 31 143 + 113 Gaps:23 14.95 910 71.32 3e-56 DNA topoisomerase 3-alpha
blastp_kegg lcl|pop:POPTR_0005s06810g 31 143 + 113 Gaps:23 14.95 910 72.06 9e-56 POPTRDRAFT_861019 hypothetical protein
blastp_kegg lcl|rcu:RCOM_1519060 31 143 + 113 Gaps:23 16.33 833 69.12 7e-55 prokaryotic DNA topoisomerase putative (EC:5.99.1.2)
blastp_kegg lcl|cmo:103486821 31 143 + 113 Gaps:23 14.50 938 70.59 8e-55 DNA topoisomerase 3-alpha
blastp_kegg lcl|csv:101224272 31 143 + 113 Gaps:23 22.41 607 69.85 8e-55 DNA topoisomerase 3-like
blastp_kegg lcl|vvi:100248256 31 143 + 113 Gaps:23 14.88 914 69.12 6e-54 DNA topoisomerase 3-alpha-like
blastp_kegg lcl|fve:101293143 31 143 + 113 Gaps:23 14.98 908 69.12 7e-54 DNA topoisomerase 3-alpha-like
blastp_uniprot_sprot sp|O70157|TOP3A_MOUSE 4 143 + 140 Gaps:32 15.15 1003 47.37 5e-27 DNA topoisomerase 3-alpha OS Mus musculus GN Top3a PE 2 SV 1
blastp_uniprot_sprot sp|Q13472|TOP3A_HUMAN 4 143 + 140 Gaps:28 15.18 1001 44.08 1e-26 DNA topoisomerase 3-alpha OS Homo sapiens GN TOP3A PE 1 SV 1
blastp_uniprot_sprot sp|Q9NG98|TOP3A_DROME 26 143 + 118 Gaps:22 10.72 1250 47.01 9e-26 DNA topoisomerase 3-alpha OS Drosophila melanogaster GN Top3alpha PE 2 SV 2
blastp_uniprot_sprot sp|O60126|TOP3_SCHPO 19 144 + 126 Gaps:20 23.47 622 37.67 2e-24 DNA topoisomerase 3 OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN top3 PE 1 SV 2
blastp_uniprot_sprot sp|Q8T2T7|TOP3_DICDI 26 144 + 119 Gaps:26 16.55 828 41.61 7e-24 DNA topoisomerase 3 OS Dictyostelium discoideum GN top3 PE 3 SV 1
blastp_uniprot_sprot sp|O61660|TOP3_CAEEL 20 144 + 125 Gaps:24 18.84 759 43.36 3e-23 DNA topoisomerase 3 OS Caenorhabditis elegans GN top-3 PE 2 SV 1
blastp_uniprot_sprot sp|P13099|TOP3_YEAST 25 143 + 119 Gaps:35 23.48 656 37.01 1e-20 DNA topoisomerase 3 OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN TOP3 PE 1 SV 1
blastp_uniprot_sprot sp|O95985|TOP3B_HUMAN 29 144 + 116 Gaps:20 15.55 862 35.07 1e-14 DNA topoisomerase 3-beta-1 OS Homo sapiens GN TOP3B PE 2 SV 1
blastp_uniprot_sprot sp|Q9Z321|TOP3B_MOUSE 29 144 + 116 Gaps:20 15.55 862 32.84 6e-13 DNA topoisomerase 3-beta-1 OS Mus musculus GN Top3b PE 2 SV 1
blastp_uniprot_sprot sp|O96651|TOP3B_DROME 29 144 + 116 Gaps:21 15.20 875 30.08 4e-11 DNA topoisomerase 3-beta OS Drosophila melanogaster GN Top3beta PE 2 SV 2
rpsblast_cdd gnl|CDD|73184 31 138 + 108 Gaps:21 33.86 381 34.88 9e-27 cd00186 TOP1Ac DNA Topoisomerase subtype IA DNA-binding ATP-binding and catalytic domain of bacterial DNA topoisomerases I and III and eukaryotic DNA topoisomerase III and eubacterial and archael reverse gyrases. Topoisomerases clevage single or double stranded DNA and then rejoin the broken phosphodiester backbone. Proposed catalytic mechanism of single stranded DNA cleavage is by phosphoryl transfer through a tyrosine nucleophile using acid/base catalysis. Tyr is activated by a nearby group (not yet identified) acting as a general base for nucleophilic attack on the 5' phosphate of the scissile bond. Arg and Lys stabilize the pentavalent transition state. Glu then acts as a proton donor for the leaving 3'-oxygen upon cleavage of the scissile strand..
rpsblast_cdd gnl|CDD|197725 31 138 + 108 Gaps:22 50.19 259 33.08 7e-25 smart00437 TOP1Ac Bacterial DNA topoisomerase I DNA-binding domain. Bacterial DNA topoisomerase I and III Eukaryotic DNA topoisomeraes III reverse gyrase alpha subunit.
rpsblast_cdd gnl|CDD|180891 29 133 + 105 Gaps:23 15.09 822 36.29 4e-22 PRK07219 PRK07219 DNA topoisomerase I Validated.
rpsblast_cdd gnl|CDD|201614 31 138 + 108 Gaps:18 34.52 365 32.54 4e-21 pfam01131 Topoisom_bac DNA topoisomerase. This subfamily of topoisomerase is divided on the basis that these enzymes preferentially relax negatively supercoiled DNA from a 5' phospho- tyrosine linkage in the enzyme-DNA covalent intermediate and has high affinity for single stranded DNA.
rpsblast_cdd gnl|CDD|30896 31 138 + 108 Gaps:17 21.93 570 31.20 6e-21 COG0550 TopA Topoisomerase IA [DNA replication recombination and repair].
rpsblast_cdd gnl|CDD|162179 32 134 + 103 Gaps:21 19.74 618 31.15 5e-15 TIGR01057 topA_arch DNA topoisomerase I archaeal. This model describes topoisomerase I from archaea. These enzymes are involved in the control of DNA topology. DNA topoisomerase I belongs to the type I topoisomerases which are ATP-independent.
rpsblast_cdd gnl|CDD|184936 26 133 + 108 Gaps:20 13.46 936 38.10 1e-14 PRK14973 PRK14973 DNA topoisomerase I Provisional.
rpsblast_cdd gnl|CDD|180251 32 138 + 107 Gaps:38 19.25 670 40.31 1e-14 PRK05776 PRK05776 DNA topoisomerase I Provisional.
rpsblast_cdd gnl|CDD|180892 26 133 + 108 Gaps:20 17.03 740 31.75 2e-14 PRK07220 PRK07220 DNA topoisomerase I Validated.
rpsblast_cdd gnl|CDD|181093 32 139 + 108 Gaps:23 19.91 658 26.72 7e-08 PRK07726 PRK07726 DNA topoisomerase III Provisional.

8 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
Gene3D 70 138 69 G3DSA:2.70.20.10 none none IPR013825
PANTHER 31 142 112 PTHR11390:SF23 none none none
ProSitePatterns 34 48 15 PS00396 none Prokaryotic DNA topoisomerase I active site. IPR023406
SUPERFAMILY 26 140 115 SSF56712 none none IPR023405
Gene3D 28 56 29 G3DSA:1.10.290.10 none none IPR013826
SMART 5 167 163 SM00437 none Bacterial DNA topoisomerase I DNA-binding domain IPR003602
Pfam 28 139 112 PF01131 none DNA topoisomerase IPR013497
PANTHER 31 142 112 PTHR11390 none none IPR000380

0 Localization

5 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Bourran2_2014_nSecLBD_A4 Qrob_Chr07 7 v_8327_222 s_1A4WGY_363 16,04 0 44,69 lod 2,6373 6,5
Bourran2_2014_nP_A4 Qrob_Chr11 11 s_1B58GB_1413 s_1A5BYY_1671 11,15 0 42,38 lod 1,8913 4,5
Bourran2_2015_nSeqBC_3P Qrob_Chr11 11 s_1DG9PM_867 s_1BZ083_1312 26,06 25,47 27,72 lod 3.6 7.1
Bourran2_2015_nEpis_A4 Qrob_Chr09 9 v_15847_485 v_8329_369 34,94 34,88 37,45 lod 3.1 7
Bourran2_2015_nEpiBC_A4 Qrob_Chr07 7 s_1DP9TW_798 v_8128_173 22,61 22,14 22,73 lod 3.1 8.5

0 Targeting