Protein : Qrob_P0393580.2 Q. robur

Protein Identifier  ? Qrob_P0393580.2 Organism . Name  Quercus robur
Score  0.0 Score Type  egn
Protein Description  (M=89) 3.1.1.11 - Pectinesterase. Code Enzyme  EC:3.1.1.11
Gene Prediction Quality  validated Protein length 

Sequence

Length: 171  

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0 Synonyms

2 GO Terms

Identifier Name Description
GO:0030599 pectinesterase activity Catalysis of the reaction: pectin + n H2O = n methanol + pectate.
GO:0004857 enzyme inhibitor activity Binds to and stops, prevents or reduces the activity of an enzyme.

29 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|vvi:100854576 1 170 + 170 Gaps:21 97.93 193 47.09 7e-44 pectinesterase 1-like
blastp_kegg lcl|rcu:RCOM_1433910 3 170 + 168 Gaps:2 97.67 172 42.86 9e-36 Pectinesterase inhibitor putative
blastp_kegg lcl|cic:CICLE_v10002648mg 1 170 + 170 Gaps:10 97.75 178 39.66 2e-27 hypothetical protein
blastp_kegg lcl|atr:s00088p00049450 10 167 + 158 Gaps:29 93.94 165 45.16 2e-25 AMTR_s00088p00049450 hypothetical protein
blastp_kegg lcl|pmum:103333985 1 170 + 170 Gaps:25 96.92 195 32.80 3e-25 pectinesterase inhibitor 2
blastp_kegg lcl|pper:PRUPE_ppa011794mg 1 170 + 170 Gaps:25 96.92 195 32.80 2e-24 hypothetical protein
blastp_kegg lcl|vvi:100854979 25 170 + 146 Gaps:3 81.22 181 36.73 8e-24 pectinesterase inhibitor-like
blastp_kegg lcl|pop:POPTR_0006s13710g 23 170 + 148 Gaps:6 79.57 186 36.49 4e-23 hypothetical protein
blastp_kegg lcl|mus:103972954 23 170 + 148 Gaps:5 77.44 195 37.09 3e-22 pectinesterase inhibitor-like
blastp_kegg lcl|cmo:103492143 17 142 + 126 Gaps:2 77.30 163 41.27 4e-22 pectinesterase inhibitor-like
blastp_pdb 1xg2_B 21 170 + 150 Gaps:2 98.04 153 32.67 3e-16 mol:protein length:153 Pectinesterase inhibitor
blastp_uniprot_sprot sp|P83326|PMEI_ACTDE 7 170 + 164 Gaps:2 88.65 185 31.10 4e-16 Pectinesterase inhibitor OS Actinidia deliciosa GN PMEI PE 1 SV 2
blastp_uniprot_sprot sp|Q8GT41|PLA1_PLAAC 38 170 + 133 Gaps:10 74.30 179 34.59 2e-12 Putative invertase inhibitor OS Platanus acerifolia PE 1 SV 1
blastp_uniprot_sprot sp|Q9LUV1|PMEI2_ARATH 4 170 + 167 Gaps:4 94.22 173 28.22 2e-11 Pectinesterase inhibitor 2 OS Arabidopsis thaliana GN PMEI2 PE 1 SV 1
blastp_uniprot_sprot sp|Q9LUL8|PME26_ARATH 25 166 + 142 Gaps:15 16.22 968 26.75 5e-09 Putative pectinesterase/pectinesterase inhibitor 26 OS Arabidopsis thaliana GN PME26 PE 2 SV 1
blastp_uniprot_sprot sp|Q9FHN5|PME59_ARATH 30 170 + 141 Gaps:9 27.99 536 28.00 7e-09 Probable pectinesterase/pectinesterase inhibitor 59 OS Arabidopsis thaliana GN PME59 PE 2 SV 1
blastp_uniprot_sprot sp|Q9M3B0|PME34_ARATH 26 166 + 141 Gaps:15 24.41 598 31.51 1e-07 Probable pectinesterase/pectinesterase inhibitor 34 OS Arabidopsis thaliana GN PME34 PE 2 SV 1
blastp_uniprot_sprot sp|P17407|21KD_DAUCA 5 170 + 166 Gaps:26 94.30 193 28.57 1e-07 21 kDa protein OS Daucus carota PE 2 SV 1
blastp_uniprot_sprot sp|O49603|CVIF2_ARATH 5 170 + 166 Gaps:16 97.78 180 26.70 6e-07 Cell wall / vacuolar inhibitor of fructosidase 2 OS Arabidopsis thaliana GN C/VIF2 PE 1 SV 1
blastp_uniprot_sprot sp|Q9M9W7|PME22_ARATH 1 170 + 170 Gaps:31 37.02 543 22.89 1e-06 Putative pectinesterase/pectinesterase inhibitor 22 OS Arabidopsis thaliana GN PME22 PE 3 SV 1
blastp_uniprot_sprot sp|Q9FK05|PME61_ARATH 13 166 + 154 Gaps:19 26.75 587 28.03 2e-06 Probable pectinesterase/pectinesterase inhibitor 61 OS Arabidopsis thaliana GN PME61 PE 1 SV 1
rpsblast_cdd gnl|CDD|162447 1 170 + 170 Gaps:8 98.88 178 35.23 6e-29 TIGR01614 PME_inhib pectinesterase inhibitor domain. This model describes a plant domain of about 200 amino acids characterized by four conserved Cys residues shown in a pectinesterase inhibitor from Kiwi to form two disulfide bonds: first to second and third to fourth. Roughly half the members of this family have the region described by this HMM followed immediately by a pectinesterase domain pfam01095. This suggests that the pairing of the enzymatic domain and its inhibitor reflects a conserved regulatory mechanism for this enzyme family.
rpsblast_cdd gnl|CDD|197924 23 166 + 144 Gaps:3 97.97 148 40.69 8e-25 smart00856 PMEI Plant invertase/pectin methylesterase inhibitor. This domain inhibits pectin methylesterases (PMEs) and invertases through formation of a non-covalent 1:1 complex. It has been implicated in the regulation of fruit development carbohydrate metabolism and cell wall extension. It may also be involved in inhibiting microbial pathogen PMEs. It has been observed that it is often expressed as a large inactive preprotein. It is also found at the N-termini of PMEs predicted from DNA sequences suggesting that both PMEs and their inhibitors are expressed as a single polyprotein and subsequently processed. It has two disulphide bridges and is mainly alpha-helical.
rpsblast_cdd gnl|CDD|202862 23 166 + 144 Gaps:3 100.00 145 38.62 1e-24 pfam04043 PMEI Plant invertase/pectin methylesterase inhibitor. This domain inhibits pectin methylesterases (PMEs) and invertases through formation of a non-covalent 1:1 complex. It has been implicated in the regulation of fruit development carbohydrate metabolism and cell wall extension. It may also be involved in inhibiting microbial pathogen PMEs. It has been observed that it is often expressed as a large inactive preprotein. It is also found at the N-termini of PMEs predicted from DNA sequences (personal obs:C Yeats) suggesting that both PMEs and their inhibitor are expressed as a single polyprotein and subsequently processed. It has two disulphide bridges and is mainly alpha-helical.
rpsblast_cdd gnl|CDD|178102 17 166 + 150 Gaps:17 25.72 587 29.14 3e-13 PLN02484 PLN02484 probable pectinesterase/pectinesterase inhibitor.
rpsblast_cdd gnl|CDD|178346 25 170 + 146 Gaps:7 25.34 596 27.15 4e-11 PLN02745 PLN02745 Putative pectinesterase/pectinesterase inhibitor.
rpsblast_cdd gnl|CDD|178122 7 170 + 164 Gaps:19 34.08 537 22.95 2e-10 PLN02506 PLN02506 putative pectinesterase/pectinesterase inhibitor.
rpsblast_cdd gnl|CDD|177948 30 166 + 137 Gaps:26 26.11 586 30.07 4e-10 PLN02314 PLN02314 pectinesterase.
rpsblast_cdd gnl|CDD|178574 7 170 + 164 Gaps:12 32.65 539 27.27 1e-08 PLN02995 PLN02995 Probable pectinesterase/pectinesterase inhibitor.

11 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
PANTHER 25 170 146 PTHR31707 none none none
Phobius 1 22 22 SIGNAL_PEPTIDE none Signal peptide region none
Phobius 1 3 3 SIGNAL_PEPTIDE_N_REGION none N-terminal region of a signal peptide. none
SMART 21 166 146 SM00856 "KEGG:00040+3.1.1.11","KEGG:00500+3.1.1.11","MetaCyc:PWY-1081","MetaCyc:PWY-7246","MetaCyc:PWY-7248","UniPathway:UPA00545" Plant invertase/pectin methylesterase inhibitor IPR006501
Pfam 23 166 144 PF04043 "KEGG:00040+3.1.1.11","KEGG:00500+3.1.1.11","MetaCyc:PWY-1081","MetaCyc:PWY-7246","MetaCyc:PWY-7248","UniPathway:UPA00545" Plant invertase/pectin methylesterase inhibitor IPR006501
Phobius 16 22 7 SIGNAL_PEPTIDE_C_REGION none C-terminal region of a signal peptide. none
Phobius 4 15 12 SIGNAL_PEPTIDE_H_REGION none Hydrophobic region of a signal peptide. none
Phobius 23 170 148 NON_CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. none
SUPERFAMILY 23 170 148 SSF101148 "KEGG:00040+3.1.1.11","KEGG:00500+3.1.1.11","MetaCyc:PWY-1081","MetaCyc:PWY-7246","MetaCyc:PWY-7248","UniPathway:UPA00545" none IPR006501
TIGRFAM 2 170 169 TIGR01614 "KEGG:00040+3.1.1.11","KEGG:00500+3.1.1.11","MetaCyc:PWY-1081","MetaCyc:PWY-7246","MetaCyc:PWY-7248","UniPathway:UPA00545" PME_inhib: pectinesterase inhibitor domain IPR006501
Gene3D 23 170 148 G3DSA:1.20.140.40 "KEGG:00040+3.1.1.11","KEGG:00500+3.1.1.11","MetaCyc:PWY-1081","MetaCyc:PWY-7246","MetaCyc:PWY-7248","UniPathway:UPA00545" none IPR006501

2 Localization

Analysis Start End Length
SignalP_GRAM_POSITIVE 1 29 28
SignalP_EUK 1 22 21

0 Qtllist

0 Targeting