Protein : Qrob_P0280300.2 Q. robur
Protein Identifier  ? Qrob_P0280300.2 Organism . Name  Quercus robur
Score  100.0 Score Type  egn
Protein Description  (M=1) 4.2.1.70//5.4.99.12 - Pseudouridylate synthase. // tRNA pseudouridine(38-40) synthase. Code Enzyme  EC:5.4.99.12, EC:4.2.1.70
Gene Prediction Quality  validated Protein length 

Sequence

Length: 481  
Kegg Orthology  K06173
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0 Synonyms

4 GO Terms

Identifier Name Description
GO:0003723 RNA binding Interacting selectively and non-covalently with an RNA molecule or a portion thereof.
GO:0001522 pseudouridine synthesis The intramolecular conversion of uridine to pseudouridine within an RNA molecule. This posttranscriptional base modification occurs in tRNA, rRNA, and snRNAs.
GO:0009451 RNA modification The covalent alteration of one or more nucleotides within an RNA molecule to produce an RNA molecule with a sequence that differs from that coded genetically.
GO:0009982 pseudouridine synthase activity Catalysis of the reaction: RNA uridine = RNA pseudouridine. Conversion of uridine in an RNA molecule to pseudouridine by rotation of the C1'-N-1 glycosidic bond of uridine in RNA to a C1'-C5.

32 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|tcc:TCM_042258 1 480 + 480 Gaps:35 100.00 507 64.10 0.0 Pseudouridine synthase family protein
blastp_kegg lcl|pper:PRUPE_ppa004352mg 1 480 + 480 Gaps:43 100.00 515 64.27 0.0 hypothetical protein
blastp_kegg lcl|vvi:100241658 1 477 + 477 Gaps:41 91.49 564 63.57 0.0 putative tRNA pseudouridine synthase-like
blastp_kegg lcl|pmum:103329259 1 480 + 480 Gaps:43 100.00 515 64.08 0.0 putative tRNA pseudouridine synthase
blastp_kegg lcl|pop:POPTR_0013s15030g 1 480 + 480 Gaps:57 100.00 505 62.97 0.0 POPTRDRAFT_239920 hypothetical protein
blastp_kegg lcl|fve:101308454 37 474 + 438 Gaps:34 92.73 509 62.71 0.0 putative tRNA pseudouridine synthase-like
blastp_kegg lcl|mdm:103447306 1 480 + 480 Gaps:51 97.92 530 61.27 0.0 putative tRNA pseudouridine synthase
blastp_kegg lcl|pxb:103958944 1 480 + 480 Gaps:52 97.93 531 61.35 0.0 putative tRNA pseudouridine synthase
blastp_kegg lcl|cam:101507349 1 470 + 470 Gaps:39 98.76 483 62.26 0.0 putative tRNA pseudouridine synthase-like
blastp_kegg lcl|cmo:103497029 1 465 + 465 Gaps:51 99.21 508 58.93 0.0 putative tRNA pseudouridine synthase
blastp_uniprot_sprot sp|O22928|PUSH_ARATH 1 465 + 465 Gaps:40 94.31 510 55.09 2e-166 Putative tRNA pseudouridine synthase OS Arabidopsis thaliana GN At2g30320 PE 3 SV 1
blastp_uniprot_sprot sp|Q6CC39|PUS1_YARLI 53 391 + 339 Gaps:49 64.77 528 29.82 1e-23 tRNA pseudouridine synthase 1 OS Yarrowia lipolytica (strain CLIB 122 / E 150) GN PUS1 PE 3 SV 1
blastp_uniprot_sprot sp|Q755C8|PUS1_ASHGO 53 391 + 339 Gaps:50 64.20 528 32.74 4e-23 tRNA pseudouridine synthase 1 OS Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) GN PUS1 PE 3 SV 2
blastp_uniprot_sprot sp|Q6CWQ8|PUS1_KLULA 53 383 + 331 Gaps:47 59.67 553 30.00 2e-22 tRNA pseudouridine synthase 1 OS Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) GN PUS1 PE 3 SV 1
blastp_uniprot_sprot sp|Q12211|PUS1_YEAST 41 181 + 141 Gaps:19 26.10 544 40.14 3e-16 tRNA pseudouridine synthase 1 OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN PUS1 PE 1 SV 1
blastp_uniprot_sprot sp|Q6FV05|PUS1_CANGA 53 181 + 129 Gaps:19 23.68 549 41.54 5e-16 tRNA pseudouridine synthase 1 OS Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) GN PUS1 PE 3 SV 1
blastp_uniprot_sprot sp|Q4KM92|TRUA_RAT 53 391 + 339 Gaps:44 62.17 423 35.74 2e-15 tRNA pseudouridine synthase A mitochondrial OS Rattus norvegicus GN Pus1 PE 3 SV 2
blastp_uniprot_sprot sp|Q9WU56|TRUA_MOUSE 53 391 + 339 Gaps:44 62.17 423 35.36 4e-15 tRNA pseudouridine synthase A mitochondrial OS Mus musculus GN Pus1 PE 1 SV 2
blastp_uniprot_sprot sp|O94396|PUS1_SCHPO 53 174 + 122 Gaps:18 22.85 534 36.89 1e-14 tRNA pseudouridine synthase 1 OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN pus1 PE 1 SV 1
blastp_uniprot_sprot sp|Q9Y606|TRUA_HUMAN 53 213 + 161 Gaps:32 39.11 427 29.94 7e-14 tRNA pseudouridine synthase A mitochondrial OS Homo sapiens GN PUS1 PE 1 SV 3
rpsblast_cdd gnl|CDD|178627 2 465 + 464 Gaps:35 96.88 513 62.17 0.0 PLN03078 PLN03078 Putative tRNA pseudouridine synthase Provisional.
rpsblast_cdd gnl|CDD|211335 58 386 + 329 Gaps:26 100.00 245 39.18 9e-43 cd02568 PseudoU_synth_PUS1_PUS2 Pseudouridine synthase PUS1/ PUS2 like. This group consists of eukaryotic pseudouridine synthases similar to Saccharomyces cerevisiae Pus1p S. cerevisiae Pus2p Caenorhabditis elegans Pus1p and human PUS1. Pseudouridine synthases catalyze the isomerization of specific uridines in an RNA molecule to pseudouridines (5-ribosyluracil psi). No cofactors are required. S. cerevisiae Pus1p catalyzes the formation of psi34 and psi36 in the intron-containing tRNAIle psi35 in the intron-containing tRNATyr psi27 and/or psi28 in several yeast cytoplasmic tRNAs and psi44 in U2 small nuclear RNA (U2 snRNA). The presence of the intron is required for the formation of psi 34 35 and 36. In addition S. cerevisiae PUS1 makes are psi 26 65 and 67. C. elegans Pus1p does not modify psi44 in U2 snRNA. Mouse Pus1p makes psi27/28 in pre- tRNASer tRNAVal and tRNAIle psi 34/36 in tRNAIle and psi 32 and potentially 67 in tRNAVal. Psi44 in U2 snRNA and psi32 in tRNAs are highly phylogenetically conserved. Psi 26 27 28 34 35 36 65 and 67 in tRNAs are less highly conserved. Mouse Pus1p regulates nuclear receptor activity through pseudouridylation of Steroid Receptor RNA Activator. Missense mutation in human PUS1 causes mitochondrial myopathy and sideroblastic anemia (MLASA).
rpsblast_cdd gnl|CDD|161691 54 213 + 160 Gaps:37 63.88 227 30.34 1e-18 TIGR00071 hisT_truA tRNA pseudouridine(38-40) synthase. Members of this family are the tRNA modification enzyme TruA tRNA pseudouridine(38-40) synthase. In a few species (e.g. Bacillus anthracis) TruA is represented by two paralogs.
rpsblast_cdd gnl|CDD|30450 54 381 + 328 Gaps:29 80.83 266 26.51 4e-18 COG0101 TruA Pseudouridylate synthase [Translation ribosomal structure and biogenesis].
rpsblast_cdd gnl|CDD|211322 60 213 + 154 Gaps:42 60.47 215 31.54 6e-12 cd00497 PseudoU_synth_TruA_like Pseudouridine synthase TruA family. This group consists of eukaryotic bacterial and archeal pseudouridine synthases similar to Escherichia coli TruA Saccharomyces cerevisiae Pus1p S. cerevisiae Pus3p Caenorhabditis elegans Pus1p and human PUS1. Pseudouridine synthases catalyze the isomerization of specific uridines in an RNA molecule to pseudouridines (5-ribosyluracil psi). No cofactors are required. S. cerevisiae PUS1 catalyzes the formation of psi34 and psi36 in the intron containing tRNAIle psi35 in the intron containing tRNATyr psi27 and/or psi28 in several yeast cytoplasmic tRNAs and psi44 in U2 small nuclear RNA (U2 snRNA). The presence of the intron is required for the formation of psi 34 35 and 36. In addition S. cerevisiae PUS1 makes psi 26 65 and 67. C. elegans Pus1p does not modify psi44 in U2 snRNA. S. cerevisiae Pus3p makes psi38 and psi39 in tRNAs. Psi44 in U2 snRNA and psi38 and psi39 in tRNAs are highly phylogenetically conserved. Psi 26 27 28 34 35 36 65 and 67 in tRNAs are less highly conserved. Mouse Pus1p regulates nuclear receptor activity through pseudouridylation of Steroid Receptor RNA Activator. Missense mutation in human PUS1 causes mitochondrial myopathy and sideroblastic anemia (MLASA).
rpsblast_cdd gnl|CDD|178798 54 212 + 159 Gaps:34 63.52 244 25.16 9e-12 PRK00021 truA tRNA pseudouridine synthase A Validated.
rpsblast_cdd gnl|CDD|211337 58 173 + 116 Gaps:29 45.61 239 32.11 1e-09 cd02570 PseudoU_synth_EcTruA Eukaryotic and bacterial pseudouridine synthases similar to E. coli TruA. This group consists of eukaryotic and bacterial pseudouridine synthases similar to E. coli TruA Pseudomonas aeruginosa truA and human pseudouridine synthase-like 1 (PUSL1). Pseudouridine synthases catalyze the isomerization of specific uridines in an RNA molecule to pseudouridines (5-ribosyluracil psi). No cofactors are required. E. coli TruA makes psi38/39 and/or 40 in tRNA. psi38 and psi39 in tRNAs are highly phylogenetically conserved. P. aeruginosa truA is required for induction of type III secretory genes and may act through modifying tRNAs critical for the expression of type III genes or their regulators.
rpsblast_cdd gnl|CDD|211336 58 212 + 155 Gaps:45 61.72 256 27.85 3e-09 cd02569 PseudoU_synth_ScPus3 Pseudouridine synthase Saccharomyces cerevisiae Pus3 like. This group consists of eukaryotic pseudouridine synthases similar to S. cerevisiae Pus3p mouse Pus3p and human PUS2. Pseudouridine synthases catalyze the isomerization of specific uridines in an RNA molecule to pseudouridines (5-ribosyluracil psi). No cofactors are required. S. cerevisiae Pus3p makes psi38 and psi39 in tRNAs. Mouse Pus3p has been shown to makes psi38 and possibly also psi 39 in tRNAs. Psi38 and psi39 are highly conserved in tRNAs from eubacteria archea and eukarya.
rpsblast_cdd gnl|CDD|211343 58 217 + 160 Gaps:41 62.56 219 31.39 4e-08 cd02866 PseudoU_synth_TruA_Archea Archeal pseudouridine synthases. This group consists of archeal pseudouridine synthases.Pseudouridine synthases catalyze the isomerization of specific uridines in an RNA molecule to pseudouridines (5-ribosyluracil psi). No cofactors are required. This group of proteins make Psedouridine in tRNAs.
rpsblast_cdd gnl|CDD|173053 55 212 + 158 Gaps:41 52.45 265 30.94 6e-08 PRK14589 PRK14589 tRNA pseudouridine synthase ACD Provisional.

10 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
PANTHER 296 447 152 PTHR11142:SF4 none none none
PANTHER 48 220 173 PTHR11142:SF4 none none none
PANTHER 296 447 152 PTHR11142 none none IPR001406
SUPERFAMILY 324 386 63 SSF55120 none none IPR020103
SUPERFAMILY 53 238 186 SSF55120 none none IPR020103
Gene3D 304 386 83 G3DSA:3.30.70.660 none none IPR020095
Gene3D 167 221 55 G3DSA:3.30.70.660 none none IPR020095
Gene3D 53 164 112 G3DSA:3.30.70.580 none none IPR020094
PANTHER 48 220 173 PTHR11142 none none IPR001406
Pfam 60 162 103 PF01416 none tRNA pseudouridine synthase IPR020097

0 Localization

1 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Bourran2_2014_rEpiBC*_A4 Qrob_Chr08 8 v_12498_318 v_12364_308 35,77 14,11 55,31 lod 2,9413 6,2

0 Targeting