Protein : Qrob_P0264740.2 Q. robur
Protein Identifier  ? Qrob_P0264740.2 Organism . Name  Quercus robur
Score  100.0 Score Type  egn
Protein Description  (M=1) PTHR11748//PTHR11748:SF16 - D-LACTATE DEHYDROGENASE // SUBFAMILY NOT NAMED Code Enzyme  EC:1.1.3.8, EC:1.1.3.37
Gene Prediction Quality  validated Protein length 

Sequence

Length: 595  
Paste the following link
Lists
This Protein isn't in any lists. Upload a list.

Sequence Feature Displayer

Protein Sequence Displayer

J Browse Displayer

0 Synonyms

8 GO Terms

Identifier Name Description
GO:0016491 oxidoreductase activity Catalysis of an oxidation-reduction (redox) reaction, a reversible chemical reaction in which the oxidation state of an atom or atoms within a molecule is altered. One substrate acts as a hydrogen or electron donor and becomes oxidized, while the other acts as hydrogen or electron acceptor and becomes reduced.
GO:0016020 membrane A lipid bilayer along with all the proteins and protein complexes embedded in it an attached to it.
GO:0055114 oxidation-reduction process A metabolic process that results in the removal or addition of one or more electrons to or from a substance, with or without the concomitant removal or addition of a proton or protons.
GO:0003824 catalytic activity Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic.
GO:0050660 flavin adenine dinucleotide binding Interacting selectively and non-covalently with FAD, flavin-adenine dinucleotide, the coenzyme or the prosthetic group of various flavoprotein oxidoreductase enzymes, in either the oxidized form, FAD, or the reduced form, FADH2.
GO:0008762 UDP-N-acetylmuramate dehydrogenase activity Catalysis of the reaction: UDP-N-acetylmuramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH + H+.
GO:0016614 oxidoreductase activity, acting on CH-OH group of donors Catalysis of an oxidation-reduction (redox) reaction in which a CH-OH group act as a hydrogen or electron donor and reduces a hydrogen or electron acceptor.
GO:0003885 D-arabinono-1,4-lactone oxidase activity Catalysis of the reaction: D-arabinono-1,4-lactone + O(2) = dehydro-D-arabinono-1,4-lactone + H(2)O(2) + H(+).

34 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|pmum:103320196 1 579 + 579 Gaps:4 96.80 594 76.35 0.0 L-gulonolactone oxidase
blastp_kegg lcl|cit:102613738 5 582 + 578 Gaps:7 98.29 585 76.52 0.0 L-gulonolactone oxidase-like
blastp_kegg lcl|pper:PRUPE_ppa026902mg 29 579 + 551 Gaps:2 96.65 568 78.51 0.0 hypothetical protein
blastp_kegg lcl|pop:POPTR_0018s00570g 29 582 + 554 Gaps:3 99.46 556 77.94 0.0 POPTRDRAFT_809065 hypothetical protein
blastp_kegg lcl|fve:101306399 1 582 + 582 Gaps:11 98.12 584 74.69 0.0 L-gulonolactone oxidase-like
blastp_kegg lcl|rcu:RCOM_0802540 8 580 + 573 Gaps:7 97.95 584 75.52 0.0 gulonolactone oxidase putative
blastp_kegg lcl|mdm:103441169 1 584 + 584 Gaps:3 97.65 595 72.12 0.0 L-gulonolactone oxidase
blastp_kegg lcl|cic:CICLE_v10007892mg 29 582 + 554 Gaps:2 98.93 560 76.53 0.0 hypothetical protein
blastp_kegg lcl|tcc:TCM_037165 1 582 + 582 Gaps:8 99.32 584 74.48 0.0 D-arabinono-1 4-lactone oxidase family protein
blastp_kegg lcl|vvi:100247576 7 582 + 576 Gaps:10 97.63 590 74.13 0.0 L-gulonolactone oxidase-like
blastp_pdb 3js8_A 66 222 + 157 Gaps:14 31.67 540 26.90 1e-07 mol:protein length:540 Cholesterol oxidase
blastp_uniprot_sprot sp|Q8HXW0|GGLO_PIG 52 532 + 481 Gaps:71 97.27 440 30.61 2e-27 L-gulonolactone oxidase OS Sus scrofa GN GULO PE 2 SV 3
blastp_uniprot_sprot sp|P58710|GGLO_MOUSE 52 532 + 481 Gaps:97 97.27 440 29.67 2e-24 L-gulonolactone oxidase OS Mus musculus GN Gulo PE 1 SV 3
blastp_uniprot_sprot sp|Q90YK3|GGLO_SCYTO 52 532 + 481 Gaps:73 97.27 440 27.80 2e-22 L-gulonolactone oxidase OS Scyliorhinus torazame GN GULO PE 2 SV 1
blastp_uniprot_sprot sp|Q3ZC33|GGLO_BOVIN 52 532 + 481 Gaps:24 84.09 440 29.73 7e-15 L-gulonolactone oxidase OS Bos taurus GN GULO PE 2 SV 3
blastp_uniprot_sprot sp|Q9HDX8|ALO_SCHPO 66 290 + 225 Gaps:21 46.85 461 29.63 6e-14 D-arabinono-1 4-lactone oxidase OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN alo1 PE 3 SV 1
blastp_uniprot_sprot sp|O06804|GULDH_MYCTU 52 531 + 480 Gaps:81 97.90 428 25.06 2e-13 L-gulono-1 4-lactone dehydrogenase OS Mycobacterium tuberculosis GN Rv1771 PE 1 SV 2
blastp_uniprot_sprot sp|Q7SGY1|ALO_NEUCR 66 295 + 230 Gaps:14 39.57 556 29.55 1e-12 Putative D-arabinono-1 4-lactone oxidase OS Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) GN alo-1 PE 3 SV 1
blastp_uniprot_sprot sp|P10867|GGLO_RAT 52 532 + 481 Gaps:23 79.77 440 28.21 1e-11 L-gulonolactone oxidase OS Rattus norvegicus GN Gulo PE 1 SV 3
blastp_uniprot_sprot sp|O47881|GLDH_BRAOL 52 262 + 211 Gaps:19 32.67 600 30.10 5e-11 L-galactono-1 4-lactone dehydrogenase mitochondrial OS Brassica oleracea PE 1 SV 1
blastp_uniprot_sprot sp|Q9SU56|GLDH_ARATH 52 233 + 182 Gaps:14 27.87 610 28.24 6e-10 L-galactono-1 4-lactone dehydrogenase mitochondrial OS Arabidopsis thaliana GN GLDH PE 1 SV 1
rpsblast_cdd gnl|CDD|162480 30 578 + 549 Gaps:10 100.00 557 57.27 0.0 TIGR01677 pln_FAD_oxido plant-specific FAD-dependent oxidoreductase. This model represents an uncharacterized plant-specific family of FAD-dependent oxidoreductases. At least seven distinct members are found in Arabidopsis thaliana. The family shows considerable sequence similarity to three different enzymes of ascorbic acid biosynthesis: L-galactono-1 4-lactone dehydrogenase (EC 1.3.2.3) from higher plants D-arabinono-1 4-lactone oxidase (EC 1.1.3.37 from Saccharomyces cerevisiae and L-gulonolactone oxidase (EC 1.1.3.8) from mouse as well as to a bacterial sorbitol oxidase. The class of compound acted on by members of this family is unknown.
rpsblast_cdd gnl|CDD|165679 352 578 + 227 Gaps:7 91.05 257 48.29 6e-75 PLN00107 PLN00107 FAD-dependent oxidoreductase Provisional.
rpsblast_cdd gnl|CDD|30625 52 524 + 473 Gaps:59 93.68 459 18.14 2e-23 COG0277 GlcD FAD/FMN-containing dehydrogenases [Energy production and conversion].
rpsblast_cdd gnl|CDD|162481 66 522 + 457 Gaps:22 64.61 438 33.22 3e-20 TIGR01678 FAD_lactone_ox sugar 1 4-lactone oxidases. This model represents a family of at least two different sugar 1 4 lactone oxidases both involved in synthesizing ascorbic acid or a derivative. These include L-gulonolactone oxidase (EC 1.1.3.8) from rat and D-arabinono-1 4-lactone oxidase (EC 1.1.3.37) from Saccharomyces cerevisiae. Members are proposed to have the cofactor FAD covalently bound at a site specified by Prosite motif PS00862 OX2_COVAL_FAD 1.
rpsblast_cdd gnl|CDD|146588 432 526 + 95 Gaps:5 36.43 258 32.98 3e-14 pfam04030 ALO D-arabinono-1 4-lactone oxidase. This domain is specific to D-arabinono-1 4-lactone oxidase EC:1.1.3.- which is involved in the final step of the D-erythroascorbic acid biosynthesis pathway.
rpsblast_cdd gnl|CDD|201863 63 193 + 131 Gaps:13 90.65 139 26.98 2e-13 pfam01565 FAD_binding_4 FAD binding domain. This family consists of various enzymes that use FAD as a co-factor most of the enzymes are similar to oxygen oxidoreductase. One of the enzymes Vanillyl-alcohol oxidase (VAO) has a solved structure the alignment includes the FAD binding site called the PP-loop between residues 99-110. The FAD molecule is covalently bound in the known structure however the residue that links to the FAD is not in the alignment. VAO catalyzes the oxidation of a wide variety of substrates ranging form aromatic amines to 4-alkylphenols. Other members of this family include D-lactate dehydrogenase this enzyme catalyzes the conversion of D-lactate to pyruvate using FAD as a co-factor mitomycin radical oxidase this enzyme oxidises the reduced form of mitomycins and is involved in mitomycin resistance. This family includes MurB an UDP-N-acetylenolpyruvoylglucosamine reductase enzyme EC:1.1.1.158. This enzyme is involved in the biosynthesis of peptidoglycan.
rpsblast_cdd gnl|CDD|178084 51 233 + 183 Gaps:26 29.84 573 28.65 5e-11 PLN02465 PLN02465 L-galactono-1 4-lactone dehydrogenase.
rpsblast_cdd gnl|CDD|130740 52 233 + 182 Gaps:19 39.86 419 32.34 8e-11 TIGR01679 bact_FAD_ox FAD-linked oxidoreductase. This model represents a family of bacterial oxidoreductases with covalently linked FAD closely related to two different eukaryotic oxidases L-gulonolactone oxidase (EC 1.1.3.8) from rat and D-arabinono-1 4-lactone oxidase (EC 1.1.3.37) from Saccharomyces cerevisiae.
rpsblast_cdd gnl|CDD|130737 66 262 + 197 Gaps:19 33.64 541 29.67 4e-10 TIGR01676 GLDHase galactonolactone dehydrogenase. This model represents L-Galactono-gamma-lactone dehydrogenase (EC 1.3.2.3). This enzyme catalyzes the final step in ascorbic acid biosynthesis in higher plants. This protein is homologous to ascorbic acid biosynthesis enzymes of other species: L-gulono-gamma-lactone oxidase in rat and L-galactono-gamma-lactone oxidase in yeast. All three covalently bind the cofactor FAD.

14 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
PANTHER 42 550 509 PTHR11748 none none none
Pfam 63 194 132 PF01565 "KEGG:00520+1.3.1.98","KEGG:00550+1.3.1.98","MetaCyc:PWY-6386","MetaCyc:PWY-6387","UniPathway:UPA00219" FAD binding domain IPR006094
Phobius 1 6 6 SIGNAL_PEPTIDE_N_REGION none N-terminal region of a signal peptide. none
Gene3D 119 236 118 G3DSA:3.30.465.10 none none IPR016169
Gene3D 52 110 59 G3DSA:3.30.43.10 none none IPR016167
PANTHER 42 550 509 PTHR11748:SF16 none none none
Phobius 18 25 8 SIGNAL_PEPTIDE_C_REGION none C-terminal region of a signal peptide. none
TIGRFAM 30 578 549 TIGR01677 "KEGG:00053+1.1.3.8","UniPathway:UPA00132" pln_FAD_oxido: plant-specific FAD-dependent oxidoreductase IPR010030
ProSiteProfiles 56 238 183 PS51387 none PCMH-type FAD-binding domain profile. IPR016166
Phobius 26 594 569 NON_CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. none
Pfam 421 528 108 PF04030 none D-arabinono-1,4-lactone oxidase IPR007173
SUPERFAMILY 49 235 187 SSF56176 none none IPR016166
Phobius 1 25 25 SIGNAL_PEPTIDE none Signal peptide region none
Phobius 7 17 11 SIGNAL_PEPTIDE_H_REGION none Hydrophobic region of a signal peptide. none

2 Localization

Analysis Start End Length
SignalP_EUK 1 20 19
TMHMM 7 29 22

3 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Bourran2_2015_nEpiBC_3P Qrob_Chr12 12 s_1B73S5_217 v_7050_211 28,31 26,37 28,45 lod 4.5 11.6
Bourran2_2015_nP_3P Qrob_Chr12 12 v_10140_295 v_838_303 16 14,69 18,01 lod 5.1 13.7
Bourran2_2015_nSecLBD_3P Qrob_Chr12 12 s_A4YYC_633 s_2F1IZB_703 18,64 18,01 21,53 lod 6.5 17.2

1 Targeting

Analysis Start End Length Location Reliability Signal Peptide Cut Off Mitochondrion Cut Off Network Signal Peptide Length
TargetP 1 20   Secretory pathway 1 0.968 0.014 NON-PLANT 20