Protein : Qrob_P0243560.2 Q. robur

Protein Identifier  ? Qrob_P0243560.2 Organism . Name  Quercus robur
Score  43.1 Score Type  egn
Protein Description  (M=27) 1.3.3.8 - Tetrahydroberberine oxidase. Code Enzyme  EC:1.3.3.8
Gene Prediction Quality  validated Protein length 

Sequence

Length: 521  

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0 Synonyms

6 GO Terms

Identifier Name Description
GO:0016491 oxidoreductase activity Catalysis of an oxidation-reduction (redox) reaction, a reversible chemical reaction in which the oxidation state of an atom or atoms within a molecule is altered. One substrate acts as a hydrogen or electron donor and becomes oxidized, while the other acts as hydrogen or electron acceptor and becomes reduced.
GO:0055114 oxidation-reduction process A metabolic process that results in the removal or addition of one or more electrons to or from a substance, with or without the concomitant removal or addition of a proton or protons.
GO:0003824 catalytic activity Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic.
GO:0050660 flavin adenine dinucleotide binding Interacting selectively and non-covalently with FAD, flavin-adenine dinucleotide, the coenzyme or the prosthetic group of various flavoprotein oxidoreductase enzymes, in either the oxidized form, FAD, or the reduced form, FADH2.
GO:0008762 UDP-N-acetylmuramate dehydrogenase activity Catalysis of the reaction: UDP-N-acetylmuramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH + H+.
GO:0016614 oxidoreductase activity, acting on CH-OH group of donors Catalysis of an oxidation-reduction (redox) reaction in which a CH-OH group act as a hydrogen or electron donor and reduces a hydrogen or electron acceptor.

34 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|pxb:103952422 7 516 + 510 Gaps:15 98.30 530 66.60 0.0 reticuline oxidase-like protein
blastp_kegg lcl|pper:PRUPE_ppa019761mg 8 516 + 509 Gaps:16 99.24 525 65.45 0.0 hypothetical protein
blastp_kegg lcl|pxb:103952423 7 516 + 510 Gaps:16 98.48 528 62.12 0.0 reticuline oxidase-like protein
blastp_kegg lcl|pop:POPTR_0011s15940g 1 515 + 515 Gaps:15 99.44 531 63.45 0.0 POPTRDRAFT_233054 FAD-binding domain-containing family protein
blastp_kegg lcl|pper:PRUPE_ppa004103mg 6 516 + 511 Gaps:15 98.87 530 60.88 0.0 hypothetical protein
blastp_kegg lcl|pmum:103326620 1 516 + 516 Gaps:16 100.00 530 60.75 0.0 reticuline oxidase-like protein
blastp_kegg lcl|pper:PRUPE_ppa016294mg 6 516 + 511 Gaps:15 98.87 530 60.88 0.0 hypothetical protein
blastp_kegg lcl|pper:PRUPE_ppa018650mg 15 516 + 502 Gaps:15 100.00 513 62.18 0.0 hypothetical protein
blastp_kegg lcl|pper:PRUPE_ppa022216mg 6 516 + 511 Gaps:15 98.87 530 60.88 0.0 hypothetical protein
blastp_kegg lcl|rcu:RCOM_0770830 20 517 + 498 Gaps:17 97.52 524 62.43 0.0 Reticuline oxidase precursor putative (EC:1.21.3.3)
blastp_pdb 3d2j_A 1 519 + 519 Gaps:37 97.03 538 38.89 4e-102 mol:protein length:538 berberine bridge-forming enzyme
blastp_pdb 3d2h_A 1 519 + 519 Gaps:37 97.03 538 38.89 4e-102 mol:protein length:538 berberine bridge-forming enzyme
blastp_pdb 3d2d_A 1 519 + 519 Gaps:37 97.03 538 38.89 4e-102 mol:protein length:538 berberine bridge-forming enzyme
blastp_pdb 3fw9_A 48 516 + 469 Gaps:30 96.77 495 40.50 3e-101 mol:protein length:495 Reticuline oxidase
blastp_pdb 3gsy_A 48 519 + 472 Gaps:30 92.87 519 40.25 5e-101 mol:protein length:519 Reticuline oxidase Berberine bridge-forming
blastp_pdb 3fwa_A 48 519 + 472 Gaps:30 96.98 497 40.04 4e-100 mol:protein length:497 Reticuline oxidase
blastp_pdb 3fw8_A 48 516 + 469 Gaps:30 96.77 495 40.29 6e-100 mol:protein length:495 Reticuline oxidase
blastp_pdb 3fw7_A 48 516 + 469 Gaps:30 96.18 498 40.29 7e-100 mol:protein length:498 Reticuline oxidase
blastp_pdb 3rja_A 70 516 + 447 Gaps:45 93.87 473 27.48 4e-24 mol:protein length:473 Carbohydrate oxidase
blastp_pdb 3rj8_A 70 516 + 447 Gaps:45 93.87 473 27.48 4e-24 mol:protein length:473 Carbohydrate oxidase
blastp_uniprot_sprot sp|Q9SVG4|RETOL_ARATH 25 516 + 492 Gaps:20 88.42 570 48.81 6e-152 Reticuline oxidase-like protein OS Arabidopsis thaliana GN At4g20830 PE 1 SV 2
blastp_uniprot_sprot sp|Q8GTB6|THCAS_CANSA 17 519 + 503 Gaps:28 95.60 545 47.22 3e-150 Tetrahydrocannabinolic acid synthase OS Cannabis sativa PE 1 SV 1
blastp_uniprot_sprot sp|A6P6V9|CBDAS_CANSA 20 517 + 498 Gaps:29 94.67 544 47.77 5e-148 Cannabidiolic acid synthase OS Cannabis sativa GN CBDAS PE 1 SV 1
blastp_uniprot_sprot sp|Q33DQ2|THCAI_CANSA 17 519 + 503 Gaps:34 95.60 545 46.83 4e-147 Inactive tetrahydrocannabinolic acid synthase OS Cannabis sativa PE 3 SV 1
blastp_uniprot_sprot sp|A6P6W0|CASL1_CANSA 17 517 + 501 Gaps:30 95.23 545 45.86 4e-146 Cannabidiolic acid synthase-like 1 OS Cannabis sativa GN CBDAS2 PE 2 SV 1
blastp_uniprot_sprot sp|A6P6W1|CASL2_CANSA 17 517 + 501 Gaps:30 95.23 545 45.86 3e-145 Cannabidiolic acid synthase-like 2 OS Cannabis sativa GN CBDAS3 PE 2 SV 1
blastp_uniprot_sprot sp|P93479|RETO_PAPSO 7 519 + 513 Gaps:44 97.38 535 38.96 4e-102 Reticuline oxidase OS Papaver somniferum GN BBE1 PE 3 SV 1
blastp_uniprot_sprot sp|P30986|RETO_ESCCA 1 519 + 519 Gaps:37 97.03 538 38.89 1e-100 Reticuline oxidase OS Eschscholzia californica GN BBE1 PE 1 SV 1
blastp_uniprot_sprot sp|Q796Y5|YGAK_BACSU 73 516 + 444 Gaps:50 92.68 451 29.43 3e-25 Uncharacterized FAD-linked oxidoreductase YgaK OS Bacillus subtilis (strain 168) GN ygaK PE 3 SV 4
blastp_uniprot_sprot sp|O06997|YVDP_BACSU 78 515 + 438 Gaps:51 92.39 447 30.27 2e-16 Uncharacterized FAD-linked oxidoreductase YvdP OS Bacillus subtilis (strain 168) GN yvdP PE 1 SV 1

13 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
Gene3D 75 136 62 G3DSA:3.30.43.10 none none IPR016167
ProSiteProfiles 1 21 21 PS51257 none Prokaryotic membrane lipoprotein lipid attachment site profile. none
Gene3D 137 249 113 G3DSA:3.30.465.10 none none IPR016169
SUPERFAMILY 27 249 223 SSF56176 none none IPR016166
Phobius 1 6 6 SIGNAL_PEPTIDE_N_REGION none N-terminal region of a signal peptide. none
Phobius 23 520 498 NON_CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. none
PANTHER 1 520 520 PTHR32448 none none none
Phobius 1 22 22 SIGNAL_PEPTIDE none Signal peptide region none
Pfam 472 514 43 PF08031 none Berberine and berberine like IPR012951
Phobius 18 22 5 SIGNAL_PEPTIDE_C_REGION none C-terminal region of a signal peptide. none
ProSiteProfiles 74 248 175 PS51387 none PCMH-type FAD-binding domain profile. IPR016166
Phobius 7 17 11 SIGNAL_PEPTIDE_H_REGION none Hydrophobic region of a signal peptide. none
Pfam 80 215 136 PF01565 "KEGG:00520+1.3.1.98","KEGG:00550+1.3.1.98","MetaCyc:PWY-6386","MetaCyc:PWY-6387","UniPathway:UPA00219" FAD binding domain IPR006094

3 Localization

Analysis Start End Length
SignalP_GRAM_NEGATIVE 1 22 21
SignalP_GRAM_POSITIVE 1 22 21
SignalP_EUK 1 22 21

0 Qtllist

1 Targeting

Analysis Start End Length Location Reliability Signal Peptide Cut Off Mitochondrion Cut Off Network Signal Peptide Length
TargetP 1 24   Secretory pathway 1 0.953 0.031 NON-PLANT 24