Protein : Qrob_P0243510.2 Q. robur

Protein Identifier  ? Qrob_P0243510.2 Organism . Name  Quercus robur
Score  22.5 Score Type  egn
Protein Description  (M=27) 1.3.3.8 - Tetrahydroberberine oxidase. Code Enzyme  EC:1.3.3.8
Gene Prediction Quality  validated Protein length 

Sequence

Length: 529  

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0 Synonyms

6 GO Terms

Identifier Name Description
GO:0016491 oxidoreductase activity Catalysis of an oxidation-reduction (redox) reaction, a reversible chemical reaction in which the oxidation state of an atom or atoms within a molecule is altered. One substrate acts as a hydrogen or electron donor and becomes oxidized, while the other acts as hydrogen or electron acceptor and becomes reduced.
GO:0055114 oxidation-reduction process A metabolic process that results in the removal or addition of one or more electrons to or from a substance, with or without the concomitant removal or addition of a proton or protons.
GO:0003824 catalytic activity Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic.
GO:0050660 flavin adenine dinucleotide binding Interacting selectively and non-covalently with FAD, flavin-adenine dinucleotide, the coenzyme or the prosthetic group of various flavoprotein oxidoreductase enzymes, in either the oxidized form, FAD, or the reduced form, FADH2.
GO:0008762 UDP-N-acetylmuramate dehydrogenase activity Catalysis of the reaction: UDP-N-acetylmuramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH + H+.
GO:0016614 oxidoreductase activity, acting on CH-OH group of donors Catalysis of an oxidation-reduction (redox) reaction in which a CH-OH group act as a hydrogen or electron donor and reduces a hydrogen or electron acceptor.

33 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|pmum:103325297 1 527 + 527 Gaps:7 99.81 535 75.84 0.0 reticuline oxidase-like protein
blastp_kegg lcl|pper:PRUPE_ppa004021mg 1 528 + 528 Gaps:7 100.00 535 75.33 0.0 hypothetical protein
blastp_kegg lcl|pxb:103952337 1 528 + 528 Gaps:9 99.44 540 74.30 0.0 reticuline oxidase-like protein
blastp_kegg lcl|gmx:100778568 1 526 + 526 Gaps:4 99.07 535 75.09 0.0 reticuline oxidase-like protein-like
blastp_kegg lcl|mdm:103424356 1 527 + 527 Gaps:11 99.26 542 74.72 0.0 reticuline oxidase-like protein
blastp_kegg lcl|cic:CICLE_v10014838mg 24 528 + 505 Gaps:2 94.24 538 75.94 0.0 hypothetical protein
blastp_kegg lcl|cam:101508898 6 528 + 523 Gaps:5 96.53 547 74.24 0.0 reticuline oxidase-like protein-like
blastp_kegg lcl|cit:102628955 24 528 + 505 Gaps:2 94.24 538 75.74 0.0 reticuline oxidase-like protein-like
blastp_kegg lcl|cic:CICLE_v10018073mg 24 528 + 505 Gaps:2 94.77 535 75.74 0.0 hypothetical protein
blastp_kegg lcl|vvi:100252519 29 527 + 499 Gaps:1 94.16 531 76.80 0.0 reticuline oxidase-like protein-like
blastp_pdb 3d2j_A 12 528 + 517 Gaps:20 94.98 538 39.73 4e-105 mol:protein length:538 berberine bridge-forming enzyme
blastp_pdb 3d2h_A 12 528 + 517 Gaps:20 94.98 538 39.73 4e-105 mol:protein length:538 berberine bridge-forming enzyme
blastp_pdb 3d2d_A 12 528 + 517 Gaps:20 94.98 538 39.73 4e-105 mol:protein length:538 berberine bridge-forming enzyme
blastp_pdb 3gsy_A 23 528 + 506 Gaps:18 96.72 519 39.04 1e-103 mol:protein length:519 Reticuline oxidase Berberine bridge-forming
blastp_pdb 3fw9_A 30 526 + 497 Gaps:18 99.60 495 39.35 8e-103 mol:protein length:495 Reticuline oxidase
blastp_pdb 3fwa_A 30 528 + 499 Gaps:18 99.60 497 39.19 7e-102 mol:protein length:497 Reticuline oxidase
blastp_pdb 3fw7_A 26 526 + 501 Gaps:18 99.80 498 39.03 1e-101 mol:protein length:498 Reticuline oxidase
blastp_pdb 3fw8_A 30 526 + 497 Gaps:18 99.60 495 39.15 3e-101 mol:protein length:495 Reticuline oxidase
blastp_pdb 3rja_A 63 523 + 461 Gaps:51 94.71 473 26.34 3e-22 mol:protein length:473 Carbohydrate oxidase
blastp_pdb 3rj8_A 63 523 + 461 Gaps:51 94.71 473 26.34 3e-22 mol:protein length:473 Carbohydrate oxidase
blastp_uniprot_sprot sp|Q9SVG4|RETOL_ARATH 25 528 + 504 Gaps:5 88.60 570 56.83 0.0 Reticuline oxidase-like protein OS Arabidopsis thaliana GN At4g20830 PE 1 SV 2
blastp_uniprot_sprot sp|Q8GTB6|THCAS_CANSA 14 527 + 514 Gaps:17 96.70 545 49.34 7e-160 Tetrahydrocannabinolic acid synthase OS Cannabis sativa PE 1 SV 1
blastp_uniprot_sprot sp|A6P6W0|CASL1_CANSA 14 527 + 514 Gaps:17 96.70 545 47.25 1e-156 Cannabidiolic acid synthase-like 1 OS Cannabis sativa GN CBDAS2 PE 2 SV 1
blastp_uniprot_sprot sp|A6P6W1|CASL2_CANSA 14 527 + 514 Gaps:25 96.70 545 47.63 2e-156 Cannabidiolic acid synthase-like 2 OS Cannabis sativa GN CBDAS3 PE 2 SV 1
blastp_uniprot_sprot sp|Q33DQ2|THCAI_CANSA 14 527 + 514 Gaps:21 96.70 545 48.20 5e-156 Inactive tetrahydrocannabinolic acid synthase OS Cannabis sativa PE 3 SV 1
blastp_uniprot_sprot sp|A6P6V9|CBDAS_CANSA 28 527 + 500 Gaps:15 93.57 544 48.92 3e-155 Cannabidiolic acid synthase OS Cannabis sativa GN CBDAS PE 1 SV 1
blastp_uniprot_sprot sp|P93479|RETO_PAPSO 7 526 + 520 Gaps:21 96.64 535 39.85 2e-105 Reticuline oxidase OS Papaver somniferum GN BBE1 PE 3 SV 1
blastp_uniprot_sprot sp|P30986|RETO_ESCCA 12 528 + 517 Gaps:20 94.98 538 39.53 1e-103 Reticuline oxidase OS Eschscholzia californica GN BBE1 PE 1 SV 1
blastp_uniprot_sprot sp|O06997|YVDP_BACSU 77 524 + 448 Gaps:56 92.17 447 31.80 3e-26 Uncharacterized FAD-linked oxidoreductase YvdP OS Bacillus subtilis (strain 168) GN yvdP PE 1 SV 1
blastp_uniprot_sprot sp|Q796Y5|YGAK_BACSU 70 526 + 457 Gaps:53 93.13 451 27.86 5e-21 Uncharacterized FAD-linked oxidoreductase YgaK OS Bacillus subtilis (strain 168) GN ygaK PE 3 SV 4

11 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
Phobius 1 8 8 SIGNAL_PEPTIDE_N_REGION none N-terminal region of a signal peptide. none
Phobius 9 20 12 SIGNAL_PEPTIDE_H_REGION none Hydrophobic region of a signal peptide. none
SUPERFAMILY 21 248 228 SSF56176 none none IPR016166
Phobius 27 528 502 NON_CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. none
ProSiteProfiles 73 247 175 PS51387 none PCMH-type FAD-binding domain profile. IPR016166
Pfam 467 524 58 PF08031 none Berberine and berberine like IPR012951
Gene3D 133 248 116 G3DSA:3.30.465.10 none none IPR016169
PANTHER 1 528 528 PTHR32448 none none none
Phobius 1 26 26 SIGNAL_PEPTIDE none Signal peptide region none
Phobius 21 26 6 SIGNAL_PEPTIDE_C_REGION none C-terminal region of a signal peptide. none
Pfam 79 214 136 PF01565 "KEGG:00520+1.3.1.98","KEGG:00550+1.3.1.98","MetaCyc:PWY-6386","MetaCyc:PWY-6387","UniPathway:UPA00219" FAD binding domain IPR006094

2 Localization

Analysis Start End Length
SignalP_GRAM_POSITIVE 1 26 25
SignalP_EUK 1 26 25

0 Qtllist

1 Targeting

Analysis Start End Length Location Reliability Signal Peptide Cut Off Mitochondrion Cut Off Network Signal Peptide Length
TargetP 1 26   Secretory pathway 1 0.941 0.072 NON-PLANT 26