Protein : Qrob_P0228250.2 Q. robur
Protein Identifier  ? Qrob_P0228250.2 Organism . Name  Quercus robur
Score  100.0 Score Type  egn
Protein Description  (M=1) 2.8.4.5 - tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-methylthiotransferase. Code Enzyme  EC:2.8.4.5
Gene Prediction Quality  validated Protein length 

Sequence

Length: 618  
Kegg Orthology  K15865
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0 Synonyms

6 GO Terms

Identifier Name Description
GO:0003824 catalytic activity Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic.
GO:0009451 RNA modification The covalent alteration of one or more nucleotides within an RNA molecule to produce an RNA molecule with a sequence that differs from that coded genetically.
GO:0016740 transferase activity Catalysis of the transfer of a group, e.g. a methyl group, glycosyl group, acyl group, phosphorus-containing, or other groups, from one compound (generally regarded as the donor) to another compound (generally regarded as the acceptor). Transferase is the systematic name for any enzyme of EC class 2.
GO:0051539 4 iron, 4 sulfur cluster binding Interacting selectively and non-covalently with a 4 iron, 4 sulfur (4Fe-4S) cluster; this cluster consists of four iron atoms, with the inorganic sulfur atoms found between the irons and acting as bridging ligands.
GO:0051536 iron-sulfur cluster binding Interacting selectively and non-covalently with an iron-sulfur cluster, a combination of iron and sulfur atoms.
GO:0043412 macromolecule modification The covalent alteration of one or more monomeric units in a polypeptide, polynucleotide, polysaccharide, or other biological macromolecule, resulting in a change in its properties.

40 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|pxb:103952560 1 617 + 617 Gaps:14 100.00 631 80.98 0.0 threonylcarbamoyladenosine tRNA methylthiotransferase-like
blastp_kegg lcl|pxb:103967851 1 617 + 617 Gaps:14 100.00 631 80.51 0.0 threonylcarbamoyladenosine tRNA methylthiotransferase-like
blastp_kegg lcl|mdm:103441821 1 617 + 617 Gaps:14 100.00 631 80.35 0.0 threonylcarbamoyladenosine tRNA methylthiotransferase
blastp_kegg lcl|tcc:TCM_036466 1 616 + 616 Gaps:20 99.84 623 81.35 0.0 Methylthiotransferase
blastp_kegg lcl|rcu:RCOM_1333590 1 616 + 616 Gaps:13 99.84 630 79.33 0.0 radical sam protein putative (EC:1.3.1.74)
blastp_kegg lcl|pmum:103319265 1 617 + 617 Gaps:18 100.00 631 80.19 0.0 threonylcarbamoyladenosine tRNA methylthiotransferase
blastp_kegg lcl|vvi:100258899 1 596 + 596 Gaps:9 96.30 622 80.63 0.0 CDK5 regulatory subunit-associated protein 1-like 1-like
blastp_kegg lcl|pop:POPTR_0013s11230g 1 616 + 616 Gaps:9 99.84 612 80.36 0.0 radical SAM domain-containing family protein
blastp_kegg lcl|cit:102617557 1 616 + 616 Gaps:14 99.84 629 78.82 0.0 threonylcarbamoyladenosine tRNA methylthiotransferase-like
blastp_kegg lcl|cmo:103498722 1 617 + 617 Gaps:17 99.84 633 75.63 0.0 threonylcarbamoyladenosine tRNA methylthiotransferase
blastp_pdb 2qgq_H 187 401 + 215 Gaps:8 70.07 304 32.39 3e-26 mol:protein length:304 Protein TM_1862
blastp_pdb 2qgq_G 187 401 + 215 Gaps:8 70.07 304 32.39 3e-26 mol:protein length:304 Protein TM_1862
blastp_pdb 2qgq_F 187 401 + 215 Gaps:8 70.07 304 32.39 3e-26 mol:protein length:304 Protein TM_1862
blastp_pdb 2qgq_E 187 401 + 215 Gaps:8 70.07 304 32.39 3e-26 mol:protein length:304 Protein TM_1862
blastp_pdb 2qgq_D 187 401 + 215 Gaps:8 70.07 304 32.39 3e-26 mol:protein length:304 Protein TM_1862
blastp_pdb 2qgq_C 187 401 + 215 Gaps:8 70.07 304 32.39 3e-26 mol:protein length:304 Protein TM_1862
blastp_pdb 2qgq_B 187 401 + 215 Gaps:8 70.07 304 32.39 3e-26 mol:protein length:304 Protein TM_1862
blastp_pdb 2qgq_A 187 401 + 215 Gaps:8 70.07 304 32.39 3e-26 mol:protein length:304 Protein TM_1862
blastp_uniprot_sprot sp|Q6P4Y0|CDKAL_XENTR 1 474 + 474 Gaps:13 86.98 553 53.22 3e-179 Threonylcarbamoyladenosine tRNA methylthiotransferase OS Xenopus tropicalis GN cdkal1 PE 2 SV 1
blastp_uniprot_sprot sp|Q6NS26|CDKAL_XENLA 1 474 + 474 Gaps:13 86.51 556 53.22 2e-178 Threonylcarbamoyladenosine tRNA methylthiotransferase OS Xenopus laevis GN cdkal1 PE 2 SV 1
blastp_uniprot_sprot sp|Q5VV42|CDKAL_HUMAN 1 473 + 473 Gaps:22 83.42 579 53.21 8e-176 Threonylcarbamoyladenosine tRNA methylthiotransferase OS Homo sapiens GN CDKAL1 PE 1 SV 1
blastp_uniprot_sprot sp|Q91WE6|CDKAL_MOUSE 1 473 + 473 Gaps:17 83.39 578 52.70 3e-174 Threonylcarbamoyladenosine tRNA methylthiotransferase OS Mus musculus GN Cdkal1 PE 2 SV 1
blastp_uniprot_sprot sp|Q7K4W1|CDKAL_DROME 1 474 + 474 Gaps:20 88.41 552 52.87 7e-174 Threonylcarbamoyladenosine tRNA methylthiotransferase OS Drosophila melanogaster GN CG6550 PE 2 SV 1
blastp_uniprot_sprot sp|Q6PG34|CDKAL_DANRE 1 474 + 474 Gaps:9 87.57 547 51.15 7e-170 Threonylcarbamoyladenosine tRNA methylthiotransferase OS Danio rerio GN cdkal1 PE 2 SV 1
blastp_uniprot_sprot sp|Q291H5|CDKAL_DROPS 1 474 + 474 Gaps:20 88.25 553 53.48 2e-169 Threonylcarbamoyladenosine tRNA methylthiotransferase OS Drosophila pseudoobscura pseudoobscura GN GA19679 PE 3 SV 1
blastp_uniprot_sprot sp|Q8MXQ7|CDKAL_CAEEL 121 471 + 351 Gaps:5 82.82 425 52.27 7e-118 Threonylcarbamoyladenosine tRNA methylthiotransferase OS Caenorhabditis elegans GN Y92H12BL.1 PE 3 SV 2
blastp_uniprot_sprot sp|O59545|AMTAB_PYRHO 52 404 + 353 Gaps:6 83.06 425 41.08 5e-90 Probable threonylcarbamoyladenosine tRNA methylthiotransferase OS Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) GN PH1875 PE 3 SV 2
blastp_uniprot_sprot sp|Q9UXX9|AMTAB_PYRAB 52 404 + 353 Gaps:6 83.06 425 40.79 3e-89 Probable threonylcarbamoyladenosine tRNA methylthiotransferase OS Pyrococcus abyssi (strain GE5 / Orsay) GN PYRAB17290 PE 3 SV 1
rpsblast_cdd gnl|CDD|162432 52 473 + 422 Gaps:11 99.76 420 46.06 1e-121 TIGR01578 MiaB-like-B MiaB-like tRNA modifying enzyme archaeal-type. This clade of sequences is closely related to MiaB a modifier of isopentenylated adenosine-37 of certain eukaryotic and bacterial tRNAs (see TIGR01574). Sequence alignments suggest that this equivalog performs the same chemical transformation as MiaB perhaps on a different (or differently modified) tRNA base substrate. This clade is a member of a subfamily (TIGR00089) and spans the archaea and eukaryotes. The only archaeal miaB-like genes are in this clade while eukaryotes have sequences described by this HMM as well as ones falling within the scope of the MiaB equivalog model.
rpsblast_cdd gnl|CDD|30966 51 474 + 424 Gaps:22 99.31 437 35.94 2e-94 COG0621 MiaB 2-methylthioadenine synthetase [Translation ribosomal structure and biogenesis].

15 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
ProSitePatterns 187 207 21 PS01278 none Methylthiotransferase radical SAM domain signature. IPR020612
Gene3D 187 407 221 G3DSA:3.80.30.20 none none IPR023404
Pfam 191 362 172 PF04055 none Radical SAM superfamily IPR007197
SUPERFAMILY 173 407 235 SSF102114 none none none
Phobius 1 589 589 NON_CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. none
ProSiteProfiles 412 474 63 PS50926 none TRAM domain profile. IPR002792
SMART 183 403 221 SM00729 none Elongator protein 3, MiaB family, Radical SAM IPR006638
TIGRFAM 52 477 426 TIGR01578 none MiaB-like-B: MiaB-like tRNA modifying enzyme, archaeal-type IPR006466
Phobius 612 617 6 CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. none
Phobius 590 611 22 TRANSMEMBRANE none Region of a membrane-bound protein predicted to be embedded in the membrane. none
Pfam 52 130 79 PF00919 none Uncharacterized protein family UPF0004 IPR013848
TIGRFAM 51 468 418 TIGR00089 none TIGR00089: radical SAM methylthiotransferase, MiaB/RimO family IPR005839
PANTHER 1 474 474 PTHR11918 none none IPR023970
ProSiteProfiles 50 158 109 PS51449 none Methylthiotransferase N-terminal domain profile. IPR013848
PANTHER 1 474 474 PTHR11918:SF45 none none none

1 Localization

Analysis Start End Length
TMHMM 590 612 22

19 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Bourran1_2003_QTL2_peak_Bud_burst_A4 Qrob_Chr02 2 s_1B0H8U_259 s_1CB1VL_554 17 0 87 lod 3,3 8,7
Bourran2_2004_QTL9_peak_Bud_burst_3P Qrob_Chr02 2 s_1C34E9_788 v_12238_322 50 25 75 lod 4,4 10,1
NancyGreenhouseCO2_2001_ambient_elevated_leaf_cellulose_QTL2_d13Cf Qrob_Chr02 2 s_1AQA4Z_1644 s_1AK5QX_947 53.67 14,01 79,68 lod 5.6594 0.03
Bourran1_2004_QTL2_peak_Bud_burst_3P Qrob_Chr02 2 s_1AW12F_382 s_1A77MR_223 42 6 64 lod 3,6 9,6
Bourran2_2002_QTL7_peak_Bud_burst_3P Qrob_Chr02 2 s_1ANG6_1446 v_11270_161 40 29 52 lod 8,1 16
Bourran2_2002_QTL9_peak_Bud_burst_A4 Qrob_Chr02 2 s_1BFNDA_375 s_1A3VA1_2139 32,5 17 62 lod 3,1 4,2
Bourran2_2003_QTL8_peak_Bud_burst_3P Qrob_Chr02 2 s_1ANG6_1446 v_11270_161 40 0 72 lod 4,4 9,9
Bourran2_2014_nP_A4 Qrob_Chr11 11 s_1B58GB_1413 s_1A5BYY_1671 11,15 0 42,38 lod 1,8913 4,5
Bourran2_2015_nP_A4 Qrob_Chr02 2 s_1A0FUE_1868 s_1A1UAI_500 20,64 20,47 21,36 lod 5.8 10.9
Bourran2_2015_nPriLBD_A4 Qrob_Chr02 2 s_1CP5DI_1183 s_1A63ZX_1277 24,87 24,63 26,18 lod 3.8 7
NancyGreenhouseCO2_2001_ambient_elevated_leaf_cellulose_QTL6_d13Cf Qrob_Chr02 2 s_1AEP21_172 v_6048_204 46.33 22,5 65,23 lod 4.972 0.03
Bourran2_2015_nEpis_A4 Qrob_Chr09 9 v_15847_485 v_8329_369 34,94 34,88 37,45 lod 3.1 7
Bourran2_2015_nSecLBD_A4 Qrob_Chr09 9 v_15847_485 v_8329_369 35,81 34,88 37,45 lod 4.4 10.4
Bourran1_2003_QTL1_peak_Bud_burst_3P Qrob_Chr02 2 s_1AR8KI_1183 s_1B0QB1_473 22 6 41 lod 4,2 11,5
Bourran1_2004_QTL3_peak_Bud_burst_A4 Qrob_Chr02 2 s_1B0H8U_259 s_1CB1VL_554 17 0 46 lod 2,9 6,4
Bourran2_2015_nEpiBC_A4 Qrob_Chr07 7 s_1DP9TW_798 v_8128_173 22,61 22,14 22,73 lod 3.1 8.5
Champenoux_2015_nEpis_A4 Qrob_Chr02 2 s_1BAGIZ_823 s_1BN4CB_644 23,06 23,06 23,06 lod 4.9 11
Champenoux_2015_nP_A4 Qrob_Chr02 2 s_1BN4CB_644 v_508_128 23,76 23,06 24,51 lod 2.8 6.2
Champenoux_2015_nPriLBD_A4 Qrob_Chr02 2 s_1CP5DI_1183 s_1A63ZX_1277 25,35 24,63 26,18 lod 4.0 8.7

0 Targeting