Protein : Qrob_P0209520.2 Q. robur
Protein Identifier  ? Qrob_P0209520.2 Organism . Name  Quercus robur
Score  100.0 Score Type  egn
Protein Description  (M=65) PF04043 - Plant invertase/pectin methylesterase inhibitor Gene Prediction Quality  validated
Protein length 

Sequence

Length: 183  
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0 Synonyms

2 GO Terms

Identifier Name Description
GO:0030599 pectinesterase activity Catalysis of the reaction: pectin + n H2O = n methanol + pectate.
GO:0004857 enzyme inhibitor activity Binds to and stops, prevents or reduces the activity of an enzyme.

18 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|tcc:TCM_005798 18 181 + 164 Gaps:1 87.30 189 67.27 4e-80 Plant invertase/pectin methylesterase inhibitor superfamily protein
blastp_kegg lcl|pop:POPTR_0014s04360g 19 181 + 163 Gaps:1 90.00 180 72.22 2e-77 invertase/pectin methylesterase inhibitor family protein
blastp_kegg lcl|mdm:103427556 1 182 + 182 Gaps:3 98.39 186 65.57 5e-75 putative invertase inhibitor
blastp_kegg lcl|mdm:103433338 1 182 + 182 Gaps:3 98.39 186 65.57 5e-75 putative invertase inhibitor
blastp_kegg lcl|pxb:103931828 1 182 + 182 Gaps:3 98.39 186 65.03 8e-74 putative invertase inhibitor
blastp_kegg lcl|cic:CICLE_v10023652mg 19 181 + 163 Gaps:1 86.63 187 68.52 1e-70 hypothetical protein
blastp_kegg lcl|cam:101511717 9 182 + 174 Gaps:2 91.01 189 59.88 8e-68 putative invertase inhibitor-like
blastp_kegg lcl|rcu:RCOM_0658820 19 182 + 164 Gaps:2 92.05 176 64.81 7e-66 Pectinesterase inhibitor putative
blastp_kegg lcl|pper:PRUPE_ppb019226mg 23 182 + 160 Gaps:1 83.25 191 62.89 9e-66 hypothetical protein
blastp_kegg lcl|fve:101308805 20 181 + 162 Gaps:2 85.56 187 65.62 1e-65 putative invertase inhibitor-like
blastp_pdb 1xg2_B 26 181 + 156 Gaps:8 96.73 153 33.78 5e-14 mol:protein length:153 Pectinesterase inhibitor
blastp_uniprot_sprot sp|Q8GT41|PLA1_PLAAC 9 182 + 174 Gaps:5 97.77 179 43.43 4e-42 Putative invertase inhibitor OS Platanus acerifolia PE 1 SV 1
blastp_uniprot_sprot sp|P83326|PMEI_ACTDE 26 181 + 156 Gaps:8 80.00 185 33.78 2e-13 Pectinesterase inhibitor OS Actinidia deliciosa GN PMEI PE 1 SV 2
blastp_uniprot_sprot sp|O49603|CVIF2_ARATH 9 166 + 158 Gaps:14 87.78 180 29.11 1e-11 Cell wall / vacuolar inhibitor of fructosidase 2 OS Arabidopsis thaliana GN C/VIF2 PE 1 SV 1
blastp_uniprot_sprot sp|Q9LUV1|PMEI2_ARATH 14 181 + 168 Gaps:12 92.49 173 25.62 1e-05 Pectinesterase inhibitor 2 OS Arabidopsis thaliana GN PMEI2 PE 1 SV 1
rpsblast_cdd gnl|CDD|162447 6 181 + 176 Gaps:15 98.31 178 46.29 3e-32 TIGR01614 PME_inhib pectinesterase inhibitor domain. This model describes a plant domain of about 200 amino acids characterized by four conserved Cys residues shown in a pectinesterase inhibitor from Kiwi to form two disulfide bonds: first to second and third to fourth. Roughly half the members of this family have the region described by this HMM followed immediately by a pectinesterase domain pfam01095. This suggests that the pairing of the enzymatic domain and its inhibitor reflects a conserved regulatory mechanism for this enzyme family.
rpsblast_cdd gnl|CDD|202862 26 177 + 152 Gaps:9 100.00 145 40.69 3e-24 pfam04043 PMEI Plant invertase/pectin methylesterase inhibitor. This domain inhibits pectin methylesterases (PMEs) and invertases through formation of a non-covalent 1:1 complex. It has been implicated in the regulation of fruit development carbohydrate metabolism and cell wall extension. It may also be involved in inhibiting microbial pathogen PMEs. It has been observed that it is often expressed as a large inactive preprotein. It is also found at the N-termini of PMEs predicted from DNA sequences (personal obs:C Yeats) suggesting that both PMEs and their inhibitor are expressed as a single polyprotein and subsequently processed. It has two disulphide bridges and is mainly alpha-helical.
rpsblast_cdd gnl|CDD|197924 24 177 + 154 Gaps:9 99.32 148 42.86 7e-22 smart00856 PMEI Plant invertase/pectin methylesterase inhibitor. This domain inhibits pectin methylesterases (PMEs) and invertases through formation of a non-covalent 1:1 complex. It has been implicated in the regulation of fruit development carbohydrate metabolism and cell wall extension. It may also be involved in inhibiting microbial pathogen PMEs. It has been observed that it is often expressed as a large inactive preprotein. It is also found at the N-termini of PMEs predicted from DNA sequences suggesting that both PMEs and their inhibitors are expressed as a single polyprotein and subsequently processed. It has two disulphide bridges and is mainly alpha-helical.

11 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
Pfam 25 177 153 PF04043 "KEGG:00040+3.1.1.11","KEGG:00500+3.1.1.11","MetaCyc:PWY-1081","MetaCyc:PWY-7246","MetaCyc:PWY-7248","UniPathway:UPA00545" Plant invertase/pectin methylesterase inhibitor IPR006501
PANTHER 46 182 137 PTHR31707 none none none
Phobius 10 20 11 SIGNAL_PEPTIDE_H_REGION none Hydrophobic region of a signal peptide. none
Phobius 21 25 5 SIGNAL_PEPTIDE_C_REGION none C-terminal region of a signal peptide. none
Phobius 26 182 157 NON_CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. none
Phobius 1 25 25 SIGNAL_PEPTIDE none Signal peptide region none
Phobius 1 9 9 SIGNAL_PEPTIDE_N_REGION none N-terminal region of a signal peptide. none
SMART 24 177 154 SM00856 "KEGG:00040+3.1.1.11","KEGG:00500+3.1.1.11","MetaCyc:PWY-1081","MetaCyc:PWY-7246","MetaCyc:PWY-7248","UniPathway:UPA00545" Plant invertase/pectin methylesterase inhibitor IPR006501
SUPERFAMILY 26 181 156 SSF101148 "KEGG:00040+3.1.1.11","KEGG:00500+3.1.1.11","MetaCyc:PWY-1081","MetaCyc:PWY-7246","MetaCyc:PWY-7248","UniPathway:UPA00545" none IPR006501
Gene3D 26 181 156 G3DSA:1.20.140.40 "KEGG:00040+3.1.1.11","KEGG:00500+3.1.1.11","MetaCyc:PWY-1081","MetaCyc:PWY-7246","MetaCyc:PWY-7248","UniPathway:UPA00545" none IPR006501
TIGRFAM 7 181 175 TIGR01614 "KEGG:00040+3.1.1.11","KEGG:00500+3.1.1.11","MetaCyc:PWY-1081","MetaCyc:PWY-7246","MetaCyc:PWY-7248","UniPathway:UPA00545" PME_inhib: pectinesterase inhibitor domain IPR006501

3 Localization

Analysis Start End Length
SignalP_GRAM_NEGATIVE 1 25 24
SignalP_EUK 1 25 24
SignalP_GRAM_POSITIVE 1 25 24

9 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Bourran2_2014_nPriLBD_3P Qrob_Chr08 8 v_5216_549 v_11837_70 12,36 0 30,43 lod 2,5806 5,1
Bourran2_2014_nSecLBD_A4 Qrob_Chr07 7 v_8327_222 s_1A4WGY_363 16,04 0 44,69 lod 2,6373 6,5
Bourran2_2014_nP_A4 Qrob_Chr11 11 s_1B58GB_1413 s_1A5BYY_1671 11,15 0 42,38 lod 1,8913 4,5
Bourran2_2015_nSeqBC_3P Qrob_Chr11 11 s_1DG9PM_867 s_1BZ083_1312 26,06 25,47 27,72 lod 3.6 7.1
Bourran2_2014_nP_3P Qrob_Chr11 11 v_11486_194 s_1AT3E_2335 7,9 0,09 30,09 lod 2,3636 5
Bourran2_2015_nEpis_A4 Qrob_Chr09 9 v_15847_485 v_8329_369 34,94 34,88 37,45 lod 3.1 7
Bourran2_2015_nEpiBC_A4 Qrob_Chr07 7 s_1DP9TW_798 v_8128_173 22,61 22,14 22,73 lod 3.1 8.5
Bourran2_2014_nEpiBC_A4 Qrob_Chr07 7 s_2FI9D9_500 s_1AXDMJ_325 12,26 0 34,9 lod 2,2306 6,1
Bourran2_2014_nPriBD_3P Qrob_Chr11 11 v_11486_194 s_1AT3E_2335 5,54 0,4 20,6 lod 2,6345 5,9

1 Targeting

Analysis Start End Length Location Reliability Signal Peptide Cut Off Mitochondrion Cut Off Network Signal Peptide Length
TargetP 1 25   Secretory pathway 1 0.941 0.046 NON-PLANT 25