Protein : Qrob_P0188060.2 Q. robur

Protein Identifier  ? Qrob_P0188060.2 Organism . Name  Quercus robur
Score  100.0 Score Type  egn
Protein Description  (M=50) K01051 - pectinesterase [EC:3.1.1.11] Code Enzyme  EC:3.1.1.11
Gene Prediction Quality  validated Protein length 

Sequence

Length: 568  
Kegg Orthology  K01051

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0 Synonyms

4 GO Terms

Identifier Name Description
GO:0005618 cell wall The rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal, most prokaryotic cells and some protozoan parasites, maintaining their shape and protecting them from osmotic lysis. In plants it is made of cellulose and, often, lignin; in fungi it is composed largely of polysaccharides; in bacteria it is composed of peptidoglycan; in protozoan parasites such as Giardia species, it's made of carbohydrates and proteins.
GO:0030599 pectinesterase activity Catalysis of the reaction: pectin + n H2O = n methanol + pectate.
GO:0042545 cell wall modification The series of events leading to chemical and structural alterations of an existing cell wall that can result in loosening, increased extensibility or disassembly.
GO:0004857 enzyme inhibitor activity Binds to and stops, prevents or reduces the activity of an enzyme.

40 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|cic:CICLE_v10000705mg 23 566 + 544 Gaps:8 94.77 574 74.08 0.0 hypothetical protein
blastp_kegg lcl|cit:102615875 23 566 + 544 Gaps:8 94.77 574 74.08 0.0 probable pectinesterase/pectinesterase inhibitor 25-like
blastp_kegg lcl|rcu:RCOM_1541960 32 566 + 535 Gaps:9 93.52 571 77.15 0.0 Pectinesterase-2 precursor putative (EC:3.1.1.11)
blastp_kegg lcl|pop:POPTR_0010s25400g 1 566 + 566 Gaps:20 100.00 574 71.60 0.0 POPTRDRAFT_1092849 hypothetical protein
blastp_kegg lcl|pop:POPTR_0008s01180g 6 566 + 561 Gaps:13 98.10 579 71.65 0.0 POPTRDRAFT_420048 hypothetical protein
blastp_kegg lcl|pper:PRUPE_ppa003474mg 33 566 + 534 Gaps:6 93.71 572 73.32 0.0 hypothetical protein
blastp_kegg lcl|pmum:103331045 33 566 + 534 Gaps:6 93.71 572 73.69 0.0 probable pectinesterase/pectinesterase inhibitor 25
blastp_kegg lcl|vvi:100262623 28 566 + 539 Gaps:2 94.71 567 71.14 0.0 probable pectinesterase/pectinesterase inhibitor 25-like
blastp_kegg lcl|pxb:103931478 33 567 + 535 Gaps:10 93.25 578 72.91 0.0 probable pectinesterase/pectinesterase inhibitor 47
blastp_kegg lcl|pxb:103931469 33 567 + 535 Gaps:10 93.25 578 72.91 0.0 probable pectinesterase/pectinesterase inhibitor 47
blastp_pdb 1xg2_A 249 567 + 319 Gaps:4 100.00 317 53.00 1e-113 mol:protein length:317 Pectinesterase 1
blastp_pdb 1gq8_A 253 565 + 313 Gaps:4 97.49 319 55.31 4e-113 mol:protein length:319 PECTINESTERASE
blastp_pdb 2ntq_B 263 523 + 261 Gaps:60 86.84 342 33.67 9e-23 mol:protein length:342 Pectinesterase A
blastp_pdb 2ntq_A 263 523 + 261 Gaps:60 86.84 342 33.67 9e-23 mol:protein length:342 Pectinesterase A
blastp_pdb 2ntp_B 263 523 + 261 Gaps:60 86.84 342 33.67 9e-23 mol:protein length:342 Pectinesterase A
blastp_pdb 2ntp_A 263 523 + 261 Gaps:60 86.84 342 33.67 9e-23 mol:protein length:342 Pectinesterase A
blastp_pdb 2ntb_B 263 523 + 261 Gaps:60 86.84 342 33.67 9e-23 mol:protein length:342 Pectinesterase A
blastp_pdb 2ntb_A 263 523 + 261 Gaps:60 86.84 342 33.67 9e-23 mol:protein length:342 Pectinesterase A
blastp_pdb 1qjv_B 263 523 + 261 Gaps:60 86.84 342 34.01 1e-22 mol:protein length:342 PECTIN METHYLESTERASE
blastp_pdb 1qjv_A 263 523 + 261 Gaps:60 86.84 342 34.01 1e-22 mol:protein length:342 PECTIN METHYLESTERASE
blastp_uniprot_sprot sp|Q9FF77|PME47_ARATH 32 567 + 536 Gaps:6 85.90 624 67.35 0.0 Probable pectinesterase/pectinesterase inhibitor 47 OS Arabidopsis thaliana GN PME47 PE 2 SV 1
blastp_uniprot_sprot sp|Q94CB1|PME25_ARATH 32 567 + 536 Gaps:6 86.59 619 65.49 0.0 Probable pectinesterase/pectinesterase inhibitor 25 OS Arabidopsis thaliana GN PME25 PE 2 SV 1
blastp_uniprot_sprot sp|Q8RXK7|PME41_ARATH 8 566 + 559 Gaps:24 98.95 573 52.56 0.0 Probable pectinesterase/pectinesterase inhibitor 41 OS Arabidopsis thaliana GN PME41 PE 2 SV 2
blastp_uniprot_sprot sp|Q9SRX4|PME7_ARATH 5 567 + 563 Gaps:33 99.48 579 50.52 0.0 Probable pectinesterase/pectinesterase inhibitor 7 OS Arabidopsis thaliana GN PME7 PE 2 SV 1
blastp_uniprot_sprot sp|O22256|PME20_ARATH 1 566 + 566 Gaps:37 99.82 560 50.27 4e-172 Probable pectinesterase/pectinesterase inhibitor 20 OS Arabidopsis thaliana GN PME20 PE 2 SV 2
blastp_uniprot_sprot sp|Q9LXK7|PME32_ARATH 98 567 + 470 Gaps:44 85.01 527 50.45 5e-136 Probable pectinesterase/pectinesterase inhibitor 32 OS Arabidopsis thaliana GN PME32 PE 2 SV 1
blastp_uniprot_sprot sp|Q43062|PME_PRUPE 98 563 + 466 Gaps:46 82.38 522 50.70 2e-134 Pectinesterase/pectinesterase inhibitor PPE8B OS Prunus persica PE 2 SV 1
blastp_uniprot_sprot sp|P85076|PME_ACTDE 249 565 + 317 Gaps:4 98.13 321 61.90 5e-133 Pectinesterase OS Actinidia deliciosa PE 1 SV 1
blastp_uniprot_sprot sp|O04887|PME2_CITSI 13 565 + 553 Gaps:74 99.02 510 48.91 6e-130 Pectinesterase 2 OS Citrus sinensis GN PECS-2.1 PE 2 SV 1
blastp_uniprot_sprot sp|Q9SMY7|PME44_ARATH 72 565 + 494 Gaps:47 90.86 525 46.96 8e-130 Probable pectinesterase/pectinesterase inhibitor 44 OS Arabidopsis thaliana GN PME44 PE 2 SV 2

16 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
Gene3D 252 562 311 G3DSA:2.160.20.10 none none IPR012334
TIGRFAM 8 182 175 TIGR01614 "KEGG:00040+3.1.1.11","KEGG:00500+3.1.1.11","MetaCyc:PWY-1081","MetaCyc:PWY-7246","MetaCyc:PWY-7248","UniPathway:UPA00545" PME_inhib: pectinesterase inhibitor domain IPR006501
PANTHER 25 567 543 PTHR31707 none none none
Pfam 253 549 297 PF01095 "KEGG:00040+3.1.1.11","KEGG:00500+3.1.1.11","MetaCyc:PWY-1081","MetaCyc:PWY-7246","MetaCyc:PWY-7248","UniPathway:UPA00545" Pectinesterase IPR000070
Phobius 1 26 26 SIGNAL_PEPTIDE none Signal peptide region none
Phobius 6 21 16 SIGNAL_PEPTIDE_H_REGION none Hydrophobic region of a signal peptide. none
Gene3D 30 182 153 G3DSA:1.20.140.40 "KEGG:00040+3.1.1.11","KEGG:00500+3.1.1.11","MetaCyc:PWY-1081","MetaCyc:PWY-7246","MetaCyc:PWY-7248","UniPathway:UPA00545" none IPR006501
SUPERFAMILY 253 564 312 SSF51126 none none IPR011050
Phobius 27 567 541 NON_CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. none
SMART 24 180 157 SM00856 "KEGG:00040+3.1.1.11","KEGG:00500+3.1.1.11","MetaCyc:PWY-1081","MetaCyc:PWY-7246","MetaCyc:PWY-7248","UniPathway:UPA00545" Plant invertase/pectin methylesterase inhibitor IPR006501
Pfam 24 179 156 PF04043 "KEGG:00040+3.1.1.11","KEGG:00500+3.1.1.11","MetaCyc:PWY-1081","MetaCyc:PWY-7246","MetaCyc:PWY-7248","UniPathway:UPA00545" Plant invertase/pectin methylesterase inhibitor IPR006501
Phobius 1 5 5 SIGNAL_PEPTIDE_N_REGION none N-terminal region of a signal peptide. none
PANTHER 25 567 543 PTHR31707:SF7 none none none
Phobius 22 26 5 SIGNAL_PEPTIDE_C_REGION none C-terminal region of a signal peptide. none
SUPERFAMILY 31 183 153 SSF101148 "KEGG:00040+3.1.1.11","KEGG:00500+3.1.1.11","MetaCyc:PWY-1081","MetaCyc:PWY-7246","MetaCyc:PWY-7248","UniPathway:UPA00545" none IPR006501
ProSitePatterns 399 408 10 PS00503 "KEGG:00040+3.1.1.11","KEGG:00500+3.1.1.11","MetaCyc:PWY-1081","MetaCyc:PWY-7246","MetaCyc:PWY-7248","UniPathway:UPA00545" Pectinesterase signature 2. IPR018040

4 Localization

Analysis Start End Length
SignalP_EUK 1 24 23
SignalP_GRAM_NEGATIVE 1 26 25
TMHMM 7 24 17
SignalP_GRAM_POSITIVE 1 26 25

6 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Bourran2_2015_nSeqBC_3P Qrob_Chr11 11 s_1DG9PM_867 s_1BZ083_1312 26,06 25,47 27,72 lod 3.6 7.1
Bourran2_2015_nP_3P Qrob_Chr12 12 v_10140_295 v_838_303 16 14,69 18,01 lod 5.1 13.7
Bourran1_2004_QTL4_peak_Bud_burst_3P Qrob_Chr04 4 s_1AHUWN_1101 s_1BRNG7_1618 30,55 0 47,55 lod 2,8 7,4
Bourran2_2002_QTL10_peak_Bud_burst_A4 Qrob_Chr04 4 s_1B59MJ_737 s_1BGLSD_999 15,86 0 41,66 lod 2,8 4
Bourran1_2000_QTL3_peak_Bud_burst_3P Qrob_Chr04 4 s_1A6DJQ_418 s_1A1PEC_1268 23,09 9,09 44,09 lod 2,3 6,8
NancyGreenhouseCO2_2001_ambient_elevated_leaf_cellulose_QTL7_d13Cf Qrob_Chr04 4 s_1B91MJ_705 s_1AHIKX_640 33.92 12,11 48,31 lod 6.1865 0.023

1 Targeting

Analysis Start End Length Location Reliability Signal Peptide Cut Off Mitochondrion Cut Off Network Signal Peptide Length
TargetP 1 26   Secretory pathway 1 0.959 0.032 NON-PLANT 26