Protein : Qrob_P0166080.2 Q. robur

Protein Identifier  ? Qrob_P0166080.2 Organism . Name  Quercus robur
Score  0.0 Score Type  egn
Protein Description  (M=10) PTHR31080:SF2 - F23N19.14-RELATED (PTHR31080:SF2) Code Enzyme  EC:3.1.1.11
Gene Prediction Quality  validated Protein length 

Sequence

Length: 206  

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0 Synonyms

2 GO Terms

Identifier Name Description
GO:0030599 pectinesterase activity Catalysis of the reaction: pectin + n H2O = n methanol + pectate.
GO:0004857 enzyme inhibitor activity Binds to and stops, prevents or reduces the activity of an enzyme.

30 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|pper:PRUPE_ppa011478mg 25 199 + 175 Gaps:4 84.13 208 58.86 4e-63 hypothetical protein
blastp_kegg lcl|pmum:103333339 25 202 + 178 Gaps:4 85.58 208 56.74 5e-62 21 kDa protein-like
blastp_kegg lcl|fve:101312980 43 202 + 160 none 83.77 191 58.13 3e-59 21 kDa protein-like
blastp_kegg lcl|pxb:103959872 10 202 + 193 Gaps:8 94.71 208 51.78 1e-58 21 kDa protein-like
blastp_kegg lcl|mdm:103440934 10 199 + 190 Gaps:7 92.79 208 53.37 3e-58 21 kDa protein
blastp_kegg lcl|pxb:103961999 10 202 + 193 Gaps:7 94.63 205 52.06 1e-56 21 kDa protein-like
blastp_kegg lcl|tcc:TCM_014951 10 199 + 190 Gaps:6 93.33 210 49.49 7e-54 Plant invertase/pectin methylesterase inhibitor superfamily protein putative
blastp_kegg lcl|rcu:RCOM_1500020 16 202 + 187 Gaps:2 91.75 206 53.97 1e-50 21 kDa protein precursor putative
blastp_kegg lcl|gmx:100527443 26 199 + 174 none 76.32 228 50.00 4e-50 uncharacterized LOC100527443
blastp_kegg lcl|pop:POPTR_0012s14570g 26 202 + 177 Gaps:3 86.14 202 51.72 8e-50 POPTRDRAFT_570589 invertase/pectin methylesterase inhibitor family protein
blastp_uniprot_sprot sp|P17407|21KD_DAUCA 46 202 + 157 Gaps:3 82.90 193 40.62 2e-37 21 kDa protein OS Daucus carota PE 2 SV 1
blastp_uniprot_sprot sp|O04886|PME1_CITSI 41 199 + 159 Gaps:3 27.74 584 30.86 9e-18 Pectinesterase 1 OS Citrus sinensis GN PECS-1.1 PE 2 SV 1
blastp_uniprot_sprot sp|P83948|PME3_CITSI 41 199 + 159 Gaps:3 27.74 584 30.86 1e-17 Pectinesterase 3 OS Citrus sinensis PE 1 SV 1
blastp_uniprot_sprot sp|Q42534|PME2_ARATH 47 199 + 153 Gaps:2 26.41 587 28.39 4e-15 Pectinesterase 2 OS Arabidopsis thaliana GN PME2 PE 2 SV 2
blastp_uniprot_sprot sp|Q43143|PMEU1_SOLLC 47 199 + 153 Gaps:6 26.93 583 26.75 9e-13 Pectinesterase/pectinesterase inhibitor U1 OS Solanum lycopersicum GN PMEU1 PE 2 SV 1
blastp_uniprot_sprot sp|O49006|PME3_ARATH 47 199 + 153 Gaps:4 26.18 592 26.45 4e-12 Pectinesterase/pectinesterase inhibitor 3 OS Arabidopsis thaliana GN PME3 PE 2 SV 2
blastp_uniprot_sprot sp|Q9LUL8|PME26_ARATH 23 202 + 180 Gaps:56 53.41 968 27.85 7e-11 Putative pectinesterase/pectinesterase inhibitor 26 OS Arabidopsis thaliana GN PME26 PE 2 SV 1
blastp_uniprot_sprot sp|Q43867|PME1_ARATH 34 201 + 168 Gaps:22 30.03 586 30.11 1e-09 Pectinesterase 1 OS Arabidopsis thaliana GN PME1 PE 2 SV 1
blastp_uniprot_sprot sp|Q9FK05|PME61_ARATH 47 200 + 154 Gaps:5 25.72 587 26.49 2e-09 Probable pectinesterase/pectinesterase inhibitor 61 OS Arabidopsis thaliana GN PME61 PE 1 SV 1
blastp_uniprot_sprot sp|Q9M3B0|PME34_ARATH 32 200 + 169 Gaps:15 26.09 598 30.77 3e-09 Probable pectinesterase/pectinesterase inhibitor 34 OS Arabidopsis thaliana GN PME34 PE 2 SV 1
rpsblast_cdd gnl|CDD|197924 44 198 + 155 Gaps:10 97.97 148 35.17 6e-24 smart00856 PMEI Plant invertase/pectin methylesterase inhibitor. This domain inhibits pectin methylesterases (PMEs) and invertases through formation of a non-covalent 1:1 complex. It has been implicated in the regulation of fruit development carbohydrate metabolism and cell wall extension. It may also be involved in inhibiting microbial pathogen PMEs. It has been observed that it is often expressed as a large inactive preprotein. It is also found at the N-termini of PMEs predicted from DNA sequences suggesting that both PMEs and their inhibitors are expressed as a single polyprotein and subsequently processed. It has two disulphide bridges and is mainly alpha-helical.
rpsblast_cdd gnl|CDD|162447 12 199 + 188 Gaps:14 97.75 178 32.18 3e-20 TIGR01614 PME_inhib pectinesterase inhibitor domain. This model describes a plant domain of about 200 amino acids characterized by four conserved Cys residues shown in a pectinesterase inhibitor from Kiwi to form two disulfide bonds: first to second and third to fourth. Roughly half the members of this family have the region described by this HMM followed immediately by a pectinesterase domain pfam01095. This suggests that the pairing of the enzymatic domain and its inhibitor reflects a conserved regulatory mechanism for this enzyme family.
rpsblast_cdd gnl|CDD|202862 44 198 + 155 Gaps:10 100.00 145 31.72 7e-20 pfam04043 PMEI Plant invertase/pectin methylesterase inhibitor. This domain inhibits pectin methylesterases (PMEs) and invertases through formation of a non-covalent 1:1 complex. It has been implicated in the regulation of fruit development carbohydrate metabolism and cell wall extension. It may also be involved in inhibiting microbial pathogen PMEs. It has been observed that it is often expressed as a large inactive preprotein. It is also found at the N-termini of PMEs predicted from DNA sequences (personal obs:C Yeats) suggesting that both PMEs and their inhibitor are expressed as a single polyprotein and subsequently processed. It has two disulphide bridges and is mainly alpha-helical.
rpsblast_cdd gnl|CDD|177948 34 201 + 168 Gaps:21 29.18 586 31.58 2e-16 PLN02314 PLN02314 pectinesterase.
rpsblast_cdd gnl|CDD|178102 31 200 + 170 Gaps:5 28.11 587 28.48 1e-15 PLN02484 PLN02484 probable pectinesterase/pectinesterase inhibitor.
rpsblast_cdd gnl|CDD|178087 18 200 + 183 Gaps:18 32.04 565 28.73 3e-14 PLN02468 PLN02468 putative pectinesterase/pectinesterase inhibitor.
rpsblast_cdd gnl|CDD|177947 16 199 + 184 Gaps:12 33.05 587 25.26 3e-14 PLN02313 PLN02313 Pectinesterase/pectinesterase inhibitor.
rpsblast_cdd gnl|CDD|178122 16 203 + 188 Gaps:9 34.45 537 28.65 2e-13 PLN02506 PLN02506 putative pectinesterase/pectinesterase inhibitor.
rpsblast_cdd gnl|CDD|178315 10 201 + 192 Gaps:15 33.04 566 28.34 1e-11 PLN02713 PLN02713 Probable pectinesterase/pectinesterase inhibitor.
rpsblast_cdd gnl|CDD|178301 32 205 + 174 Gaps:12 34.21 497 27.65 1e-10 PLN02698 PLN02698 Probable pectinesterase/pectinesterase inhibitor.

13 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
Phobius 15 25 11 SIGNAL_PEPTIDE_H_REGION none Hydrophobic region of a signal peptide. none
PANTHER 4 205 202 PTHR31080 none none none
Phobius 1 33 33 SIGNAL_PEPTIDE none Signal peptide region none
SMART 42 198 157 SM00856 "KEGG:00040+3.1.1.11","KEGG:00500+3.1.1.11","MetaCyc:PWY-1081","MetaCyc:PWY-7246","MetaCyc:PWY-7248","UniPathway:UPA00545" Plant invertase/pectin methylesterase inhibitor IPR006501
Gene3D 44 202 159 G3DSA:1.20.140.40 "KEGG:00040+3.1.1.11","KEGG:00500+3.1.1.11","MetaCyc:PWY-1081","MetaCyc:PWY-7246","MetaCyc:PWY-7248","UniPathway:UPA00545" none IPR006501
Pfam 44 198 155 PF04043 "KEGG:00040+3.1.1.11","KEGG:00500+3.1.1.11","MetaCyc:PWY-1081","MetaCyc:PWY-7246","MetaCyc:PWY-7248","UniPathway:UPA00545" Plant invertase/pectin methylesterase inhibitor IPR006501
Phobius 1 14 14 SIGNAL_PEPTIDE_N_REGION none N-terminal region of a signal peptide. none
Phobius 26 33 8 SIGNAL_PEPTIDE_C_REGION none C-terminal region of a signal peptide. none
Phobius 34 205 172 NON_CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. none
Coils 118 139 22 Coil none none none
SUPERFAMILY 45 203 159 SSF101148 "KEGG:00040+3.1.1.11","KEGG:00500+3.1.1.11","MetaCyc:PWY-1081","MetaCyc:PWY-7246","MetaCyc:PWY-7248","UniPathway:UPA00545" none IPR006501
TIGRFAM 15 200 186 TIGR01614 "KEGG:00040+3.1.1.11","KEGG:00500+3.1.1.11","MetaCyc:PWY-1081","MetaCyc:PWY-7246","MetaCyc:PWY-7248","UniPathway:UPA00545" PME_inhib: pectinesterase inhibitor domain IPR006501
PANTHER 4 205 202 PTHR31080:SF2 none none none

3 Localization

Analysis Start End Length
SignalP_EUK 1 33 32
TMHMM 7 29 22
SignalP_GRAM_POSITIVE 1 33 32

7 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Bourran1_2004_QTL5_peak_Bud_burst_A4 Qrob_Chr10 10 s_1C8OKQ_688 v_12844_707 25,6 13,9 39,9 lod 2,7 6,1
Bourran2_2002_QTL13_peak_Bud_burst_3P Qrob_Chr10 10 s_1B1AG7_637 s_1A3A1N_709 19,44 0 49,44 lod 3 5,6
Bourran2_2002_QTL15_peak_Bud_burst_A4 Qrob_Chr10 10 v_7579_71 s_1ATZMJ_189 23,94 0 43,64 lod 3,7 5,4
Bourran2_2014_vSeqBC_3P Qrob_Chr10 10 s_1A6CK6_610 v_7092_29 4,28 0 23,27 lod 2,8619 7
Bourran2_2014_vSeqBC_A4 Qrob_Chr10 10 s_1BAM4E_1326 s_1C0HD0_1012 36,88 7,71 46,03 lod 2,6164 7,3
Champenoux_2015_nPriLBD_3P Qrob_Chr10 10 v_15000_157 v_15000_310 15,68 15,9 15,91 lod 2.4 5.5
PM_1999_QTL16_peak_Bud_burst_3P Qrob_Chr10 10 s_1B6YNI_505 s_1A1ZO_1322 7,48 0 31,48 lod 4 7,1

1 Targeting

Analysis Start End Length Location Reliability Signal Peptide Cut Off Mitochondrion Cut Off Network Signal Peptide Length
TargetP 1 33   Secretory pathway 1 0.876 0.041 NON-PLANT 33