Protein : Qrob_P0165590.2 Q. robur
Protein Identifier  ? Qrob_P0165590.2 Organism . Name  Quercus robur
Score  72.5 Score Type  egn
Protein Description  (M=65) PF04043 - Plant invertase/pectin methylesterase inhibitor Gene Prediction Quality  validated
Protein length 

Sequence

Length: 193  
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0 Synonyms

2 GO Terms

Identifier Name Description
GO:0030599 pectinesterase activity Catalysis of the reaction: pectin + n H2O = n methanol + pectate.
GO:0004857 enzyme inhibitor activity Binds to and stops, prevents or reduces the activity of an enzyme.

17 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|cic:CICLE_v10032808mg 19 187 + 169 Gaps:2 86.67 195 63.91 1e-70 hypothetical protein
blastp_kegg lcl|pxb:103959072 1 189 + 189 Gaps:4 97.42 194 58.20 1e-66 putative invertase inhibitor
blastp_kegg lcl|pxb:103961110 1 189 + 189 Gaps:4 97.42 194 58.20 1e-66 putative invertase inhibitor
blastp_kegg lcl|mdm:103409496 1 189 + 189 Gaps:4 77.46 244 57.14 3e-66 putative invertase inhibitor
blastp_kegg lcl|mdm:103437025 2 189 + 188 Gaps:3 75.40 248 57.22 1e-65 putative invertase inhibitor
blastp_kegg lcl|pper:PRUPE_ppa023516mg 1 188 + 188 Gaps:1 93.97 199 59.89 7e-65 hypothetical protein
blastp_kegg lcl|pmum:103328888 1 189 + 189 Gaps:1 94.47 199 60.11 1e-64 putative invertase inhibitor
blastp_kegg lcl|gmx:100305474 7 192 + 186 Gaps:2 94.36 195 54.89 6e-63 uncharacterized LOC100305474
blastp_kegg lcl|pop:POPTR_0003s08480g 1 190 + 190 Gaps:14 98.98 196 54.12 3e-62 hypothetical protein
blastp_kegg lcl|gmx:100791033 7 191 + 185 Gaps:3 93.40 197 54.89 1e-61 pectinesterase inhibitor 2-like
blastp_pdb 1xg2_B 49 186 + 138 Gaps:2 88.89 153 27.94 1e-08 mol:protein length:153 Pectinesterase inhibitor
blastp_uniprot_sprot sp|Q8GT41|PLA1_PLAAC 8 186 + 179 Gaps:6 96.65 179 37.57 9e-35 Putative invertase inhibitor OS Platanus acerifolia PE 1 SV 1
blastp_uniprot_sprot sp|P83326|PMEI_ACTDE 49 186 + 138 Gaps:2 73.51 185 27.94 6e-08 Pectinesterase inhibitor OS Actinidia deliciosa GN PMEI PE 1 SV 2
blastp_uniprot_sprot sp|O49603|CVIF2_ARATH 33 188 + 156 Gaps:9 83.89 180 26.49 1e-06 Cell wall / vacuolar inhibitor of fructosidase 2 OS Arabidopsis thaliana GN C/VIF2 PE 1 SV 1
rpsblast_cdd gnl|CDD|162447 7 187 + 181 Gaps:9 97.75 178 36.21 1e-27 TIGR01614 PME_inhib pectinesterase inhibitor domain. This model describes a plant domain of about 200 amino acids characterized by four conserved Cys residues shown in a pectinesterase inhibitor from Kiwi to form two disulfide bonds: first to second and third to fourth. Roughly half the members of this family have the region described by this HMM followed immediately by a pectinesterase domain pfam01095. This suggests that the pairing of the enzymatic domain and its inhibitor reflects a conserved regulatory mechanism for this enzyme family.
rpsblast_cdd gnl|CDD|202862 32 182 + 151 Gaps:9 99.31 145 40.28 7e-24 pfam04043 PMEI Plant invertase/pectin methylesterase inhibitor. This domain inhibits pectin methylesterases (PMEs) and invertases through formation of a non-covalent 1:1 complex. It has been implicated in the regulation of fruit development carbohydrate metabolism and cell wall extension. It may also be involved in inhibiting microbial pathogen PMEs. It has been observed that it is often expressed as a large inactive preprotein. It is also found at the N-termini of PMEs predicted from DNA sequences (personal obs:C Yeats) suggesting that both PMEs and their inhibitor are expressed as a single polyprotein and subsequently processed. It has two disulphide bridges and is mainly alpha-helical.
rpsblast_cdd gnl|CDD|197924 32 182 + 151 Gaps:9 97.30 148 38.89 3e-21 smart00856 PMEI Plant invertase/pectin methylesterase inhibitor. This domain inhibits pectin methylesterases (PMEs) and invertases through formation of a non-covalent 1:1 complex. It has been implicated in the regulation of fruit development carbohydrate metabolism and cell wall extension. It may also be involved in inhibiting microbial pathogen PMEs. It has been observed that it is often expressed as a large inactive preprotein. It is also found at the N-termini of PMEs predicted from DNA sequences suggesting that both PMEs and their inhibitors are expressed as a single polyprotein and subsequently processed. It has two disulphide bridges and is mainly alpha-helical.

11 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
SMART 29 182 154 SM00856 "KEGG:00040+3.1.1.11","KEGG:00500+3.1.1.11","MetaCyc:PWY-1081","MetaCyc:PWY-7246","MetaCyc:PWY-7248","UniPathway:UPA00545" Plant invertase/pectin methylesterase inhibitor IPR006501
Phobius 1 28 28 SIGNAL_PEPTIDE none Signal peptide region none
TIGRFAM 7 186 180 TIGR01614 "KEGG:00040+3.1.1.11","KEGG:00500+3.1.1.11","MetaCyc:PWY-1081","MetaCyc:PWY-7246","MetaCyc:PWY-7248","UniPathway:UPA00545" PME_inhib: pectinesterase inhibitor domain IPR006501
Phobius 6 17 12 SIGNAL_PEPTIDE_H_REGION none Hydrophobic region of a signal peptide. none
SUPERFAMILY 32 186 155 SSF101148 "KEGG:00040+3.1.1.11","KEGG:00500+3.1.1.11","MetaCyc:PWY-1081","MetaCyc:PWY-7246","MetaCyc:PWY-7248","UniPathway:UPA00545" none IPR006501
Gene3D 32 188 157 G3DSA:1.20.140.40 "KEGG:00040+3.1.1.11","KEGG:00500+3.1.1.11","MetaCyc:PWY-1081","MetaCyc:PWY-7246","MetaCyc:PWY-7248","UniPathway:UPA00545" none IPR006501
Pfam 32 182 151 PF04043 "KEGG:00040+3.1.1.11","KEGG:00500+3.1.1.11","MetaCyc:PWY-1081","MetaCyc:PWY-7246","MetaCyc:PWY-7248","UniPathway:UPA00545" Plant invertase/pectin methylesterase inhibitor IPR006501
Phobius 1 5 5 SIGNAL_PEPTIDE_N_REGION none N-terminal region of a signal peptide. none
Phobius 29 192 164 NON_CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. none
Phobius 18 28 11 SIGNAL_PEPTIDE_C_REGION none C-terminal region of a signal peptide. none
PANTHER 45 192 148 PTHR31707 none none none

2 Localization

Analysis Start End Length
SignalP_GRAM_POSITIVE 1 23 22
SignalP_EUK 1 23 22

2 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Bourran2_2014_nSecLBD_3P Qrob_Chr08 8 s_1BN2OD_551 s_1B5AYF_599 17,17 0 43,51 lod 1,9229 4,4
Bourran2_2014_rEpiBC_3P Qrob_Chr05 5 s_2GDU0O_517 s_1BT8O3_239 25,81 0 55,45 lod 2,2101 5,5

1 Targeting

Analysis Start End Length Location Reliability Signal Peptide Cut Off Mitochondrion Cut Off Network Signal Peptide Length
TargetP 1 23   Secretory pathway 1 0.959 0.039 NON-PLANT 23