Protein : Qrob_P0144170.2 Q. robur
Protein Identifier  ? Qrob_P0144170.2 Organism . Name  Quercus robur
Score  42.0 Score Type  egn
Protein Description  (M=2) PTHR10670 - DNA POLYMERASE EPSILON CATALYTIC SUBUNIT A Code Enzyme  EC:2.7.7.7
Gene Prediction Quality  validated Protein length 

Sequence

Length: 464  
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0 Synonyms

7 GO Terms

Identifier Name Description
GO:0003677 DNA binding Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid).
GO:0003676 nucleic acid binding Interacting selectively and non-covalently with any nucleic acid.
GO:0006281 DNA repair The process of restoring DNA after damage. Genomes are subject to damage by chemical and physical agents in the environment (e.g. UV and ionizing radiations, chemical mutagens, fungal and bacterial toxins, etc.) and by free radicals or alkylating agents endogenously generated in metabolism. DNA is also damaged because of errors during its replication. A variety of different DNA repair pathways have been reported that include direct reversal, base excision repair, nucleotide excision repair, photoreactivation, bypass, double-strand break repair pathway, and mismatch repair pathway.
GO:0006260 DNA replication The cellular metabolic process in which a cell duplicates one or more molecules of DNA. DNA replication begins when specific sequences, known as origins of replication, are recognized and bound by initiation proteins, and ends when the original DNA molecule has been completely duplicated and the copies topologically separated. The unit of replication usually corresponds to the genome of the cell, an organelle, or a virus. The template for replication can either be an existing DNA molecule or RNA.
GO:0003887 DNA-directed DNA polymerase activity Catalysis of the reaction: deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1); the synthesis of DNA from deoxyribonucleotide triphosphates in the presence of a DNA template and a 3'hydroxyl group.
GO:0008408 3'-5' exonuclease activity Catalysis of the hydrolysis of ester linkages within nucleic acids by removing nucleotide residues from the 3' end.
GO:0008622 epsilon DNA polymerase complex A heterotetrameric DNA polymerase complex that catalyzes processive DNA synthesis in the absence of PCNA, but is further stimulated in the presence of PCNA. The complex contains a large catalytic subunit and three small subunits, and is best characterized in Saccharomyces, in which the subunits are named Pol2p, Dpb2p, Dpb3p, and Dpb4p. Some evidence suggests that DNA polymerase epsilon is the leading strand polymerase; it is also involved in nucleotide-excision repair and mismatch repair.

25 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|pper:PRUPE_ppa000038mg 262 410 + 149 none 6.78 2197 88.59 4e-81 hypothetical protein
blastp_kegg lcl|pmum:103327021 262 410 + 149 none 6.71 2219 88.59 1e-80 DNA polymerase epsilon catalytic subunit A-like
blastp_kegg lcl|mdm:103448067 262 410 + 149 none 11.56 1289 88.59 2e-80 DNA polymerase epsilon catalytic subunit A-like
blastp_kegg lcl|cic:CICLE_v10027662mg 262 410 + 149 none 6.75 2206 87.25 2e-80 hypothetical protein
blastp_kegg lcl|pop:POPTR_0004s19660g 262 410 + 149 none 6.90 2160 87.92 8e-80 DNA-directed DNA polymerase epsilon catalytic subunit family protein
blastp_kegg lcl|cit:102608212 262 410 + 149 none 6.75 2206 87.25 1e-79 DNA polymerase epsilon catalytic subunit A-like
blastp_kegg lcl|fve:101291492 255 410 + 156 none 7.04 2217 83.33 3e-79 DNA polymerase epsilon catalytic subunit A-like
blastp_kegg lcl|tcc:TCM_041469 262 410 + 149 none 6.73 2213 87.25 3e-78 DNA polymerase epsilon catalytic subunit isoform 1
blastp_kegg lcl|rcu:RCOM_0792580 262 410 + 149 none 6.69 2227 81.88 1e-75 DNA polymerase epsilon catalytic subunit putative (EC:2.7.7.7)
blastp_kegg lcl|vvi:100241432 262 410 + 149 none 6.73 2214 84.56 1e-75 POLE polymerase (DNA directed) epsilon
blastp_uniprot_sprot sp|F4HW04|DPOE1_ARATH 262 410 + 149 none 6.89 2161 84.56 2e-75 DNA polymerase epsilon catalytic subunit A OS Arabidopsis thaliana GN POL2A PE 1 SV 1
blastp_uniprot_sprot sp|F4IFN6|DPOE2_ARATH 262 410 + 149 none 6.97 2138 80.54 7e-72 DNA polymerase epsilon catalytic subunit B OS Arabidopsis thaliana GN POL2B PE 2 SV 1
blastp_uniprot_sprot sp|Q07864|DPOE1_HUMAN 263 436 + 174 Gaps:24 7.79 2286 42.70 4e-33 DNA polymerase epsilon catalytic subunit A OS Homo sapiens GN POLE PE 1 SV 5
blastp_uniprot_sprot sp|Q9WVF7|DPOE1_MOUSE 263 436 + 174 Gaps:24 7.80 2283 42.70 6e-33 DNA polymerase epsilon catalytic subunit A OS Mus musculus GN Pole PE 2 SV 3
blastp_uniprot_sprot sp|P87154|DPOE_SCHPO 262 436 + 175 Gaps:14 7.69 2199 43.79 1e-32 DNA polymerase epsilon catalytic subunit A OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN pol2 PE 2 SV 1
blastp_uniprot_sprot sp|Q4PFV5|DPOE_USTMA 263 436 + 174 Gaps:43 8.55 2305 39.59 6e-32 DNA polymerase epsilon catalytic subunit A OS Ustilago maydis (strain 521 / FGSC 9021) GN POL2 PE 3 SV 1
blastp_uniprot_sprot sp|Q4WXH8|DPOE_ASPFU 249 436 + 188 Gaps:24 8.61 2230 40.10 5e-31 DNA polymerase epsilon catalytic subunit A OS Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) GN pol2 PE 3 SV 1
blastp_uniprot_sprot sp|O93845|DPOE_EMENI 263 436 + 174 Gaps:23 6.84 2207 46.36 1e-28 DNA polymerase epsilon catalytic subunit A OS Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) GN pol2 PE 3 SV 2
blastp_uniprot_sprot sp|P0CN26|DPOE_CRYNJ 263 410 + 148 Gaps:20 7.47 2250 39.29 2e-27 DNA polymerase epsilon catalytic subunit A OS Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) GN POL2 PE 3 SV 1
blastp_uniprot_sprot sp|P0CN27|DPOE_CRYNB 263 410 + 148 Gaps:20 7.47 2250 39.29 2e-27 DNA polymerase epsilon catalytic subunit A OS Cryptococcus neoformans var. neoformans serotype D (strain B-3501A) GN POL2 PE 3 SV 1
rpsblast_cdd gnl|CDD|178372 411 463 + 53 none 16.72 317 79.25 8e-25 PLN02773 PLN02773 pectinesterase.
rpsblast_cdd gnl|CDD|202537 331 443 + 113 Gaps:26 53.15 254 28.15 4e-17 pfam03104 DNA_pol_B_exo1 DNA polymerase family B exonuclease domain. This domain has 3' to 5' exonuclease activity and adopts a ribonuclease H type fold.
rpsblast_cdd gnl|CDD|99822 374 410 + 37 none 18.14 204 78.38 4e-16 cd05779 DNA_polB_epsilon_exo DEDDy 3'-5' exonuclease domain of eukaryotic DNA polymerase epsilon a family-B DNA polymerase. The 3'-5' exonuclease domain of eukaryotic DNA polymerase epsilon. DNA polymerase epsilon is a family-B DNA polymerase with a catalytic subunit that contains a DEDDy-type DnaQ-like 3'-5' exonuclease domain. It is one of the three DNA-dependent type B DNA polymerases (alpha and delta are the other two) that have been identified as essential for nuclear DNA replication in eukaryotes. DNA polymerase epsilon plays a role in elongating the leading strand during DNA replication. It is also involved in DNA repair. The catalytic subunit contains both polymerase and 3'-5' exonuclease activities. The N-terminal exonuclease domain contains three sequence motifs termed ExoI ExoII and ExoIII with a specific YX(3)D pattern at ExoIII. These motifs are clustered around the active site and are involved in metal binding and catalysis. DNA polymerase epsilon also carries a unique large C-terminal domain with an unknown function. Phylogenetic analyses indicate that it is orthologous to the archaeal DNA polymerase B3 rather than to the eukaryotic alpha delta or zeta polymerases. The exonuclease domain of family-B polymerases contains a beta hairpin structure that plays an important role in active site switching in the event of nucleotide misincorporation.
rpsblast_cdd gnl|CDD|30766 323 443 + 121 Gaps:6 15.28 792 28.93 2e-13 COG0417 PolB DNA polymerase elongation subunit (family B) [DNA replication recombination and repair].
rpsblast_kog gnl|CDD|37009 262 410 + 149 Gaps:1 6.90 2173 62.67 1e-62 KOG1798 KOG1798 KOG1798 DNA polymerase epsilon catalytic subunit A [Replication recombination and repair].

6 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
PANTHER 262 410 149 PTHR10670:SF0 "KEGG:00230+2.7.7.7","KEGG:00240+2.7.7.7";signature_desc=DNA POLYMERASE EPSILON CATALYTIC SUBUNIT A none IPR029703
Pfam 249 420 172 PF03104 "KEGG:00230+2.7.7.7","KEGG:00240+2.7.7.7" DNA polymerase family B, exonuclease domain IPR006133
Gene3D 377 414 38 G3DSA:3.30.420.10 none none IPR012337
SUPERFAMILY 263 293 31 SSF53098 none none IPR012337
SUPERFAMILY 324 443 120 SSF53098 none none IPR012337
PANTHER 262 410 149 PTHR10670 none none none

0 Localization

2 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Bourran2_2002_QTL17_peak_Bud_burst_A4 Qrob_Chr12 12 s_1CTJ3J_556 s_1CTJ3J_556 0 0 25 lod 2,9 6
Bourran2_2014_nSeqBC_3P Qrob_Chr12 12 s_1EO8V5_710 s_1A2VMU_355 11,04 0 32,58 lod 1,7196 4,7

0 Targeting