Protein : Qrob_P0041030.2 Q. robur
Protein Identifier  ? Qrob_P0041030.2 Organism . Name  Quercus robur
Score  97.0 Score Type  egn
Protein Description  (M=1) PTHR13620:SF2 - gb def: cg6744 gene product [drosophila melanogaster] Code Enzyme  EC:3.6.4.12
Gene Prediction Quality  validated Protein length 

Sequence

Length: 590  
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0 Synonyms

3 GO Terms

Identifier Name Description
GO:0003676 nucleic acid binding Interacting selectively and non-covalently with any nucleic acid.
GO:0006139 nucleobase-containing compound metabolic process Any cellular metabolic process involving nucleobases, nucleosides, nucleotides and nucleic acids.
GO:0008408 3'-5' exonuclease activity Catalysis of the hydrolysis of ester linkages within nucleic acids by removing nucleotide residues from the 3' end.

31 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|pmum:103343964 1 577 + 577 Gaps:27 95.16 578 78.36 0.0 uncharacterized LOC103343964
blastp_kegg lcl|pper:PRUPE_ppa003579mg 1 577 + 577 Gaps:27 97.52 564 78.18 0.0 hypothetical protein
blastp_kegg lcl|mdm:103433884 1 583 + 583 Gaps:33 96.35 575 77.62 0.0 uncharacterized LOC103433884
blastp_kegg lcl|pxb:103930574 1 583 + 583 Gaps:33 96.35 575 76.53 0.0 uncharacterized LOC103930574
blastp_kegg lcl|tcc:TCM_026742 1 580 + 580 Gaps:24 96.19 578 75.54 0.0 Polynucleotidyl transferase isoform 1
blastp_kegg lcl|rcu:RCOM_1434540 1 577 + 577 Gaps:28 96.32 570 76.68 0.0 3-5 exonuclease putative
blastp_kegg lcl|mdm:103413943 1 577 + 577 Gaps:31 96.31 569 76.09 0.0 exonuclease mut-7 homolog
blastp_kegg lcl|fve:101292807 1 577 + 577 Gaps:27 98.22 562 76.27 0.0 uncharacterized protein LOC101292807
blastp_kegg lcl|cit:102615303 1 577 + 577 Gaps:24 95.67 578 72.51 0.0 uncharacterized LOC102615303
blastp_kegg lcl|pxb:103962292 1 577 + 577 Gaps:31 95.30 575 75.36 0.0 uncharacterized LOC103962292
blastp_pdb 2e6m_A 377 555 + 179 Gaps:37 73.08 208 32.89 8e-07 mol:protein length:208 Werner syndrome ATP-dependent helicase homolo
blastp_pdb 2e6l_A 377 555 + 179 Gaps:37 73.08 208 32.89 8e-07 mol:protein length:208 Werner syndrome ATP-dependent helicase homolo
blastp_pdb 2fc0_A 377 553 + 177 Gaps:35 73.17 205 35.33 6e-06 mol:protein length:205 Werner syndrome helicase
blastp_pdb 2fby_A 377 553 + 177 Gaps:35 73.17 205 35.33 6e-06 mol:protein length:205 Werner syndrome helicase
blastp_pdb 2fbx_A 377 553 + 177 Gaps:35 73.17 205 35.33 6e-06 mol:protein length:205 Werner syndrome helicase
blastp_pdb 2fbv_A 377 553 + 177 Gaps:35 73.17 205 35.33 6e-06 mol:protein length:205 Werner syndrome helicase
blastp_pdb 2fbt_A 377 553 + 177 Gaps:35 73.17 205 35.33 6e-06 mol:protein length:205 Werner syndrome helicase
blastp_uniprot_sprot sp|Q8N9H8|MUT7_HUMAN 370 549 + 180 Gaps:49 19.52 876 32.75 3e-12 Probable exonuclease mut-7 homolog OS Homo sapiens GN EXD3 PE 1 SV 3
blastp_uniprot_sprot sp|Q84LH3|WEX_ARATH 374 551 + 178 Gaps:35 52.43 288 32.45 1e-09 Werner Syndrome-like exonuclease OS Arabidopsis thaliana GN WEX PE 1 SV 1
blastp_uniprot_sprot sp|P34607|MUT7_CAEEL 378 555 + 178 Gaps:25 18.79 910 30.99 7e-07 Probable exonuclease mut-7 OS Caenorhabditis elegans GN mut-7 PE 1 SV 1
rpsblast_cdd gnl|CDD|176655 356 553 + 198 Gaps:45 98.96 193 36.13 9e-44 cd06146 mut-7_like_exo DEDDy 3'-5' exonuclease domain of Caenorhabditis elegans mut-7 and similar proteins. The mut-7 subfamily is composed of Caenorhabditis elegans mut-7 and similar proteins found in plants and metazoans. Mut-7 is implicated in posttranscriptional gene silencing. It contains a DEDDy-type DnaQ-like 3'-5' exonuclease domain possessing three conserved sequence motifs termed ExoI ExoII and ExoIII with a specific YX(3)D pattern at ExoIII. These motifs are clustered around the active site and contain four conserved acidic residues that serve as ligands for the two metal ions required for catalysis.
rpsblast_cdd gnl|CDD|176653 376 553 + 178 Gaps:44 88.24 170 36.00 4e-26 cd06141 WRN_exo DEDDy 3'-5' exonuclease domain of WRN and similar proteins. WRN is a unique RecQ DNA helicase exhibiting an exonuclease activity. It contains a DEDDy-type DnaQ-like 3'-5' exonuclease domain possessing three conserved sequence motifs termed ExoI ExoII and ExoIII with a specific YX(3)D pattern at ExoIII. These motifs are clustered around the active site and contain four conserved acidic residues that serve as ligands for the two metal ions required for catalysis. Mutations in the WRN gene cause Werner syndrome an autosomal recessive disorder associated with premature aging and increased susceptibility to cancer and type II diabetes. WRN interacts with key proteins involved in DNA replication recombination and repair. It is believed to maintain genomic stability and life span by participating in DNA processes. WRN is stimulated by Ku70/80 an important regulator of genomic stability.
rpsblast_cdd gnl|CDD|176654 421 552 + 132 Gaps:29 70.22 178 33.60 1e-14 cd06142 RNaseD_exo DEDDy 3'-5' exonuclease domain of Ribonuclease D and similar proteins. Ribonuclease (RNase) D is a bacterial enzyme involved in the maturation of small stable RNAs and the 3' maturation of tRNA. It contains a DEDDy-type DnaQ-like 3'-5' exonuclease domain possessing three conserved sequence motifs termed ExoI ExoII and ExoIII with a specific YX(3)D pattern at ExoIII. These motifs are clustered around the active site and contain four conserved acidic residues that serve as ligands for the two metal ions required for catalysis. In vivo RNase D only becomes essential upon removal of other ribonucleases. Eukaryotic RNase D homologs include yeast Rrp6p human PM/Scl-100 and the Drosophila melanogaster egalitarian protein.
rpsblast_cdd gnl|CDD|201888 356 550 + 195 Gaps:55 95.35 172 32.32 9e-14 pfam01612 DNA_pol_A_exo1 3'-5' exonuclease. This domain is responsible for the 3'-5' exonuclease proofreading activity of E. coli DNA polymerase I (polI) and other enzymes it catalyzes the hydrolysis of unpaired or mismatched nucleotides. This domain consists of the amino-terminal half of the Klenow fragment in E. coli polI it is also found in the Werner syndrome helicase (WRN) focus forming activity 1 protein (FFA-1) and ribonuclease D (RNase D). Werner syndrome is a human genetic disorder causing premature aging the WRN protein has helicase activity in the 3'-5' direction. The FFA-1 protein is required for formation of a replication foci and also has helicase activity it is a homologue of the WRN protein. RNase D is a 3'-5' exonuclease involved in tRNA processing. Also found in this family is the autoantigen PM/Scl thought to be involved in polymyositis-scleroderma overlap syndrome.
rpsblast_cdd gnl|CDD|30697 414 553 + 140 Gaps:14 37.12 361 26.87 7e-12 COG0349 Rnd Ribonuclease D [Translation ribosomal structure and biogenesis].
rpsblast_cdd gnl|CDD|176650 376 554 + 179 Gaps:42 91.30 161 28.57 9e-10 cd06129 RNaseD_like DEDDy 3'-5' exonuclease domain of RNase D WRN and similar proteins. The RNase D-like group is composed of RNase D WRN and similar proteins. They contain a DEDDy-type DnaQ-like 3'-5' exonuclease domain that contains three conserved sequence motifs termed ExoI ExoII and ExoIII with a specific YX(3)D pattern at ExoIII. These motifs are clustered around the active site and contain four conserved acidic residues that serve as ligands for the two metal ions required for catalysis. RNase D is involved in the 3'-end processing of tRNA precursors. RNase D-like proteins in eukaryotes include yeast Rrp6p human PM/Scl-100 and Drosophila melanogaster egalitarian (Egl) protein. WRN is a unique DNA helicase possessing exonuclease activity. Mutation in the WRN gene is implicated in Werner syndrome a disease associated with premature aging and increased predisposition to cancer. Yeast Rrp6p and the human Polymyositis/scleroderma autoantigen 100kDa (PM/Scl-100) are exosome-associated proteins involved in the degradation and processing of precursors to stable RNAs. Egl is a component of an mRNA-binding complex which is required for oocyte specification. The Egl subfamily does not possess a completely conserved YX(3)D pattern at the ExoIII motif.
rpsblast_cdd gnl|CDD|197748 414 550 + 137 Gaps:29 76.74 172 32.58 1e-09 smart00474 35EXOc 3'-5' exonuclease. 3\' -5' exonuclease proofreading domain present in DNA polymerase I Werner syndrome helicase RNase D and other enzymes.
rpsblast_cdd gnl|CDD|176656 452 551 + 100 Gaps:6 68.00 150 26.47 2e-07 cd09018 DEDDy_polA_RNaseD_like_exo DEDDy 3'-5' exonuclease domain of family-A DNA polymerases RNase D WRN and similar proteins. DEDDy exonucleases part of the DnaQ-like (or DEDD) exonuclease superfamily catalyze the excision of nucleoside monophosphates at the DNA or RNA termini in the 3'-5' direction. They contain four invariant acidic residues in three conserved sequence motifs termed ExoI ExoII and ExoIII. DEDDy exonucleases are classified as such because of the presence of a specific YX(3)D pattern at ExoIII. The four conserved acidic residues serve as ligands for the two metal ions required for catalysis. This family of DEDDy exonucleases includes the proofreading domains of family A DNA polymerases as well as RNases such as RNase D and yeast Rrp6p. The Egalitarian (Egl) and Bacillus-like DNA Polymerase I subfamilies do not possess a completely conserved YX(3)D pattern at the ExoIII motif. In addition the Bacillus-like DNA polymerase I subfamily has inactive 3'-5' exonuclease domains which do not possess the metal-binding residues necessary for activity.
rpsblast_cdd gnl|CDD|130455 452 552 + 101 Gaps:23 27.25 367 32.00 3e-07 TIGR01388 rnd ribonuclease D. This model describes ribonuclease D a 3'-exonuclease shown to act on tRNA both in vitro and when overexpressed in vivo. Trusted members of this family are restricted to the Proteobacteria Aquifex Mycobacterial and eukaryotic homologs are not full-length homologs. Ribonuclease D is not essential in E. coli and is deleterious when overexpressed. Its precise biological role is still unknown.
rpsblast_cdd gnl|CDD|28891 421 550 + 130 Gaps:10 83.87 155 30.00 4e-07 cd00007 35EXOc 3'-5' exonuclease. The 35EXOc domain is responsible for the 3'-5' exonuclease proofreading activity of prokaryotic DNA polymerase I (pol I) and other enzymes it catalyses the hydrolysis of unpaired or mismatched nucleotides. This domain consists of the amino-terminal half of the Klenow fragment in E. coli pol I. 35EXOc is also found in the Werner syndrome helicase (WRN) focus forming activity 1 protein (FFA-1) and ribonuclease D (RNase D)..

18 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
Phobius 44 79 36 NON_CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. none
SUPERFAMILY 331 393 63 SSF53098 none none IPR012337
SUPERFAMILY 421 552 132 SSF53098 none none IPR012337
Phobius 80 103 24 TRANSMEMBRANE none Region of a membrane-bound protein predicted to be embedded in the membrane. none
PANTHER 422 577 156 PTHR13620:SF2 none none none
Phobius 421 589 169 NON_CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. none
Pfam 420 551 132 PF01612 none 3'-5' exonuclease IPR002562
PANTHER 422 577 156 PTHR13620 none none none
Phobius 400 420 21 TRANSMEMBRANE none Region of a membrane-bound protein predicted to be embedded in the membrane. none
PANTHER 240 397 158 PTHR13620 none none none
PANTHER 28 206 179 PTHR13620 none none none
PANTHER 240 397 158 PTHR13620:SF2 none none none
PANTHER 28 206 179 PTHR13620:SF2 none none none
Gene3D 371 553 183 G3DSA:3.30.420.10 none none IPR012337
Phobius 1 20 20 CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. none
Phobius 21 43 23 TRANSMEMBRANE none Region of a membrane-bound protein predicted to be embedded in the membrane. none
Phobius 104 399 296 CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. none
Coils 103 124 22 Coil none none none

3 Localization

Analysis Start End Length
TMHMM 79 101 22
TMHMM 399 421 22
TMHMM 21 43 22

6 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Bourran2_2014_nLBD*_A4 Qrob_Chr08 8 v_12498_318 v_12364_308 34,91 16,12 53,62 lod 2,4961 5,2
Bourran2_2014_rEpiBC*_A4 Qrob_Chr08 8 v_12498_318 v_12364_308 35,77 14,11 55,31 lod 2,9413 6,2
Bourran_2000_2002_QTL6_Delta.F Qrob_Chr03 3 s_2HYXJ2_207 s_1AUAXK_317 29.12 6,3 45,37 lod 3.1666 0.026
Bourran2_2014_nLBD_3P Qrob_Chr03 3 s_1ET7H8_604 s_1BYNB5_834 23,73 0 46,72 lod 1,7573 3,8
PM_1999_QTL14_peak_Bud_burst_3P Qrob_Chr03 3 s_2F0SI1_756 v_6056_735 11,78 3,78 50,78 lod 2,5 4,5
Bourran2_2014_rEpiBC*_3P Qrob_Chr03 3 s_1A3B3X_1163 s_1DKGMO_450 23,04 4,92 41,12 lod 2,5668 7

0 Targeting