Protein : Qrob_P0032890.2 Q. robur
Protein Identifier  ? Qrob_P0032890.2 Organism . Name  Quercus robur
Score  98.1 Score Type  egn
Protein Description  (M=4) 3.1.26.4 - Ribonuclease H. Code Enzyme  EC:3.1.26.4
Gene Prediction Quality  validated Protein length 

Sequence

Length: 312  
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0 Synonyms

2 GO Terms

Identifier Name Description
GO:0003676 nucleic acid binding Interacting selectively and non-covalently with any nucleic acid.
GO:0004523 RNA-DNA hybrid ribonuclease activity Catalysis of the endonucleolytic cleavage of RNA in RNA-DNA hybrids to 5'-phosphomonoesters.

36 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|mdm:103442281 1 307 + 307 Gaps:56 98.78 329 62.15 4e-126 uncharacterized LOC103442281
blastp_kegg lcl|pxb:103941404 1 307 + 307 Gaps:36 98.71 309 64.59 1e-125 uncharacterized LOC103941404
blastp_kegg lcl|pmum:103329904 1 307 + 307 Gaps:61 98.80 334 60.30 4e-124 uncharacterized LOC103329904
blastp_kegg lcl|pxb:103936444 1 307 + 307 Gaps:56 98.78 329 61.23 5e-124 uncharacterized LOC103936444
blastp_kegg lcl|pxb:103939641 1 307 + 307 Gaps:56 98.78 329 60.92 1e-123 uncharacterized LOC103939641
blastp_kegg lcl|vvi:100242330 1 310 + 310 Gaps:27 100.00 287 68.64 3e-122 uncharacterized LOC100242330
blastp_kegg lcl|cic:CICLE_v10012229mg 1 308 + 308 Gaps:41 98.43 318 62.62 4e-122 hypothetical protein
blastp_kegg lcl|cit:102607166 1 308 + 308 Gaps:42 98.42 317 62.82 7e-121 uncharacterized LOC102607166
blastp_kegg lcl|cam:101503342 1 307 + 307 Gaps:21 76.68 373 62.94 2e-119 uncharacterized LOC101503342
blastp_kegg lcl|pop:POPTR_0004s13455g 1 308 + 308 Gaps:54 98.83 257 73.62 3e-119 POPTRDRAFT_714469 RNase H domain-containing family protein
blastp_pdb 3hst_D 171 294 + 124 Gaps:1 88.65 141 41.60 4e-21 mol:protein length:141 Protein Rv2228c/MT2287
blastp_pdb 3hst_B 171 294 + 124 Gaps:1 88.65 141 41.60 4e-21 mol:protein length:141 Protein Rv2228c/MT2287
blastp_pdb 3u3g_A 171 298 + 128 Gaps:5 95.00 140 32.33 9e-16 mol:protein length:140 Ribonuclease H
blastp_pdb 3u3g_C 171 298 + 128 Gaps:5 95.00 140 32.33 9e-16 mol:protein length:140 Ribonuclease H
blastp_pdb 3u3g_B 171 298 + 128 Gaps:5 95.00 140 32.33 9e-16 mol:protein length:140 Ribonuclease H
blastp_pdb 3u3g_D 171 298 + 128 Gaps:5 95.00 140 32.33 9e-16 mol:protein length:140 Ribonuclease H
blastp_pdb 2ehg_A 175 299 + 125 Gaps:16 86.58 149 37.21 2e-08 mol:protein length:149 ribonuclease HI
blastp_pdb 3aly_B 175 299 + 125 Gaps:16 90.21 143 37.21 2e-08 mol:protein length:143 Putative uncharacterized protein ST0753
blastp_pdb 3aly_A 175 299 + 125 Gaps:16 90.21 143 37.21 2e-08 mol:protein length:143 Putative uncharacterized protein ST0753
blastp_uniprot_sprot sp|Q9HSF6|RNH_HALSA 163 298 + 136 Gaps:4 70.35 199 40.00 1e-20 Ribonuclease HI OS Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) GN rnhA PE 1 SV 1
blastp_uniprot_sprot sp|P64956|Y2253_MYCBO 171 294 + 124 Gaps:1 34.34 364 41.60 3e-19 Uncharacterized protein Mb2253c OS Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) GN Mb2253c PE 4 SV 1
blastp_uniprot_sprot sp|P64955|Y2228_MYCTU 171 294 + 124 Gaps:1 34.34 364 41.60 3e-19 Uncharacterized protein Rv2228c/MT2287 OS Mycobacterium tuberculosis GN Rv2228c PE 1 SV 1
blastp_uniprot_sprot sp|P54162|RNHL_BACSU 176 301 + 126 Gaps:3 93.18 132 31.71 9e-10 14.7 kDa ribonuclease H-like protein OS Bacillus subtilis (strain 168) GN rnhA PE 1 SV 1
blastp_uniprot_sprot sp|A4SXM6|RNH_POLSQ 167 297 + 131 Gaps:18 91.56 154 37.59 7e-09 Ribonuclease H OS Polynucleobacter necessarius subsp. asymbioticus (strain DSM 18221 / CIP 109841 / QLW-P1DMWA-1) GN rnhA PE 3 SV 1
blastp_uniprot_sprot sp|F9VN79|RNH_SULTO 175 299 + 125 Gaps:16 86.58 149 37.21 6e-08 Ribonuclease HI OS Sulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) GN rnhA PE 1 SV 1
blastp_uniprot_sprot sp|P36921|EBSB_ENTFA 176 297 + 122 Gaps:5 89.63 135 29.75 4e-07 Cell wall enzyme EbsB OS Enterococcus faecalis (strain ATCC 700802 / V583) GN ebsB PE 3 SV 2
blastp_uniprot_sprot sp|P0C2F6|RNHX1_ARATH 176 302 + 127 Gaps:7 20.32 620 34.92 5e-07 Putative ribonuclease H protein At1g65750 OS Arabidopsis thaliana GN At1g65750 PE 3 SV 1
rpsblast_cdd gnl|CDD|187703 171 296 + 126 Gaps:2 98.44 128 50.00 1e-40 cd09279 RNase_HI_archaeal_like RNAse HI family that includes Archaeal RNase HI. Ribonuclease H (RNase H) is classified into two evolutionarily unrelated families type 1 (prokaryotic RNase HI eukaryotic RNase H1 and viral RNase H) and type 2 (prokaryotic RNase HII and HIII and eukaryotic RNase H2). RNase H is an endonuclease that cleaves the RNA strand of an RNA/DNA hybrid in a sequence non-specific manner. RNase H is involved in DNA replication repair and transcription. RNase H is widely present in various organisms including bacteria archaea and eukaryotes and most prokaryotic and eukaryotic genomes contain multiple RNase H genes. Despite the lack of amino acid sequence homology Type 1 and type 2 RNase H share a main-chain fold and steric configurations of the four acidic active-site (DEDD) residues and have the same catalytic mechanism and functions in cells. One of the important functions of RNase H is to remove Okazaki fragments during DNA replication. Most archaeal genomes contain only type 2 RNase H (RNase HII) however a few contain RNase HI as well. Although archaeal RNase HI sequences conserve the DEDD active-site motif they lack other common features important for catalytic function such as the basic protrusion region. Archaeal RNase HI homologs are more closely related to retroviral RNase HI than bacterial and eukaryotic type I RNase H in enzymatic properties.
rpsblast_cdd gnl|CDD|180903 161 296 + 136 Gaps:9 34.68 372 44.96 2e-27 PRK07238 PRK07238 bifunctional RNase H/acid phosphatase Provisional.
rpsblast_cdd gnl|CDD|187690 175 296 + 122 Gaps:1 98.37 123 39.67 5e-23 cd06222 RNase_H RNase H is an endonuclease that cleaves the RNA strand of an RNA/DNA hybrid in a sequence non-specific manner. Ribonuclease H (RNase H) enzymes are divided into two major families Type 1 and Type 2 based on amino acid sequence similarities and biochemical properties. RNase H is an endonuclease that cleaves the RNA strand of an RNA/DNA hybrid in a sequence non-specific manner in the presence of divalent cations. RNase H is widely present in various organisms including bacteria archaea and eukaryotes. Most prokaryotic and eukaryotic genomes contain multiple RNase H genes. Despite the lack of amino acid sequence homology Type 1 and type 2 RNase H share a main-chain fold and steric configurations of the four acidic active-site residues and have the same catalytic mechanism and functions in cells. RNase H is involved in DNA replication repair and transcription. One of the important functions of RNase H is to remove Okazaki fragments during DNA replication. RNase H inhibitors have been explored as an anti-HIV drug target because RNase H inactivation inhibits reverse transcription.

5 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
Gene3D 5 29 25 G3DSA:3.40.970.10 none none IPR011320
Gene3D 171 300 130 G3DSA:3.30.420.10 none none IPR012337
SUPERFAMILY 171 295 125 SSF53098 none none IPR012337
ProSiteProfiles 167 298 132 PS50879 none RNase H domain profile. IPR002156
Pfam 212 297 86 PF13456 none Reverse transcriptase-like none

0 Localization

11 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Bourran2_2014_nSecLBD_3P Qrob_Chr08 8 s_1BN2OD_551 s_1B5AYF_599 17,17 0 43,51 lod 1,9229 4,4
Bourran2_2015_nEpiBC_3P Qrob_Chr12 12 s_1B73S5_217 v_7050_211 28,31 26,37 28,45 lod 4.5 11.6
Bourran2_2014_aSeqBC_A4 Qrob_Chr08 8 v_15999_278 v_AP13YL15_395 32,52 4,22 57,22 lod 2,7561 6,7
Bourran2_2014_nFork*_A4 Qrob_Chr08 8 PIE175 s_1CD7GJ_1398 31,22 5,24 57,24 lod 2,6724 6,8
Bourran2_2014_nLBD*_3P Qrob_Chr08 8 v_5216_549 v_11837_70 12,25 0 35,55 lod 2,5951 6
Bourran2_2014_nP*_3P Qrob_Chr08 8 v_5216_549 v_11837_70 12,19 0 31,97 lod 2,8472 6
Bourran2_2014_nPriLBD_3P Qrob_Chr08 8 v_5216_549 v_11837_70 12,36 0 30,43 lod 2,5806 5,1
Bourran2_2015_rEpiBC_3P Qrob_Chr08 8 s_A9TNV_543 v_11837_70 9,93 9,83 11,15 lod 3.3 7.3
Champenoux_2015_nSeqBC_A4 Qrob_Chr08 8 v_AD7YD13_501 s_1A7IED_780 43,44 43,42 43,99 lod 3.7 8.9
Bourran2_2014_nEpis*_3P Qrob_Chr08 8 s_1DA4QW_688 s_1DNI7D_820 17,96 0 37,75 lod 2,9745 7,5
Bourran2_2014_nPriBD_3P Qrob_Chr11 11 v_11486_194 s_1AT3E_2335 5,54 0,4 20,6 lod 2,6345 5,9

0 Targeting