GRS - Genome Report System

Leptosphaeria maculans GRS

Functional annotation

Lema_P103750.1

Gene Identifier

DbXrefs

Domain/Motif (Interproscan results)

Localization / Targeting

Predicted function

Blast based analysis

Analysis	Hit	start	end	length	Note	Hit coverage	Hit length	Hit pident	Hit pcons	eValue	Hit description
blastp_kegg	ssl:SS1G_08927	101	549	449	n/a	73.49	596	64.16	13.24	0.0	hypothetical protein
	nfi:NFIA_084380	101	542	442	n/a	65.76	587	63.99	17.36	1e-160	zinc carboxypeptidase putative
	afm:AFUA_2G08790	101	542	442	n/a	72.53	586	59.53	19.29	1e-160	zinc carboxypeptidase (EC:3.4.17.-) K01302
	ang:An12g04170	87	540	454	n/a	66.83	621	61.45	17.11	1e-158	hypothetical protein
	aor:AO090011000821	102	555	454	n/a	64.57	604	62.56	15.90	1e-155	zinc carboxypeptidase
	fgr:FG01898.1	102	543	442	n/a	66.61	563	61.07	16.80	1e-148	hypothetical protein
	ani:AN6119.2	99	538	440	n/a	65.62	573	60.64	16.22	1e-147	hypothetical protein
	bfu:BC1G_10920	119	512	394	n/a	65.37	540	64.59	12.46	1e-143	hypothetical protein
	ncr:NCU01464	101	543	443	n/a	69.03	536	58.11	17.03	1e-123	hypothetical protein
	pan:PODANSg8327	210	543	334	n/a	60.30	534	59.94	17.08	1e-121	hypothetical protein
blastp_uniprot_sprot	sp\|P38836\|ECM14_YEAST	133	539	407	n/a	60.47	430	51.15	18.46	8e-66	Protein ECM14 OS Saccharomyces cerevisiae GN ECM14 PE 1 SV 1
	sp\|O74818\|YBJ7_SCHPO	101	538	438	n/a	58.35	497	39.66	22.76	2e-65	Uncharacterized carboxypeptidase C337.07c OS Schizosaccharomyces pombe GN SPBC337.07c PE 2 SV 1
	sp\|B8XGR3\|MCPA_TRIEQ	213	528	316	n/a	58.29	422	42.28	20.33	1e-54	Metallocarboxypeptidase A OS Trichophyton equinum GN MCPA PE 3 SV 1
	sp\|B6V865\|MCPA_TRITO	213	528	316	n/a	58.29	422	41.87	20.73	2e-54	Metallocarboxypeptidase A OS Trichophyton tonsurans GN MCPA PE 3 SV 1
	sp\|A6XGK3\|MCPA_TRIRU	213	528	316	n/a	58.29	422	41.46	20.73	1e-53	Metallocarboxypeptidase A OS Trichophyton rubrum GN MCPA PE 1 SV 1
	sp\|C5FVN6\|MCPA_NANOT	213	528	316	n/a	61.37	422	39.77	19.69	7e-52	Metallocarboxypeptidase A OS Nannizzia otae (strain CBS 113480) GN MCPA PE 3 SV 1
	sp\|P00732\|CBPB1_BOVIN	214	539	326	n/a	59.47	417	39.52	19.76	5e-45	Carboxypeptidase B OS Bos taurus GN CPB1 PE 1 SV 2
	sp\|P15086\|CBPB1_HUMAN	214	539	326	n/a	59.71	417	37.75	19.68	9e-43	Carboxypeptidase B OS Homo sapiens GN CPB1 PE 1 SV 4
	sp\|P09955\|CBPB1_PIG	214	542	329	n/a	60.34	416	37.45	19.52	1e-41	Carboxypeptidase B OS Sus scrofa GN CPB1 PE 1 SV 5
	sp\|Q2KIG3\|CBPB2_BOVIN	213	542	330	n/a	56.97	423	41.91	19.50	7e-41	Carboxypeptidase B2 OS Bos taurus GN CPB2 PE 1 SV 1
blastp_pdb	1zli_A	214	539	326	n/a	80.58	309	37.75	19.28	2e-43	mol:protein length:309 Carboxypeptidase B
	1kwm_B	214	539	326	n/a	61.94	402	37.75	19.28	3e-43	mol:protein length:402 Procarboxypeptidase B
	1kwm_A	214	539	326	n/a	61.94	402	37.75	19.28	3e-43	mol:protein length:402 Procarboxypeptidase B
	2pjc_C	214	542	329	n/a	82.03	306	37.45	19.52	1e-42	mol:protein length:306 Carboxypeptidase B
	2pjc_B	214	542	329	n/a	82.03	306	37.45	19.52	1e-42	mol:protein length:306 Carboxypeptidase B
	2pjc_A	214	542	329	n/a	82.03	306	37.45	19.52	1e-42	mol:protein length:306 Carboxypeptidase B
	2pjb_C	214	542	329	n/a	82.03	306	37.45	19.52	1e-42	mol:protein length:306 Carboxypeptidase B
	2pjb_B	214	542	329	n/a	82.03	306	37.45	19.52	1e-42	mol:protein length:306 Carboxypeptidase B
	2pjb_A	214	542	329	n/a	82.03	306	37.45	19.52	1e-42	mol:protein length:306 Carboxypeptidase B
	2pja_C	214	542	329	n/a	82.03	306	37.45	19.52	1e-42	mol:protein length:306 Carboxypeptidase B
rpsblast_cdd	gnl\|CDD\|133106	214	538	325	Gaps:21	100.00	304	47.37	16.45	1e-120	cd06248 M14_CPA_CPB_like Peptidase M14 Carboxypeptidase A/B-like subfamily: This is one of two main M14 carboxypeptidase subfamilies defined by sequence and structural homology the other being N/E. Carboxypeptidases (CPs) hydrolyze single C-terminal amino acids from polypeptide chains. They have a recognition site for the free C-terminal carboxyl group which is a key determinant of specificity. Majority of the proteins in this subfamily have not been characterized as yet. The A/B enzymes are normally synthesized as inactive precursors containing preceding signal peptide followed by a globular N-terminal pro-region linked to the enzyme the proenzymes are called procarboxypeptidases. These enzymes exhibit distinct substrate specificity pattern Carboxypeptidase A-like (CPA-like) enzymes favor hydrophobic residues while carboxypeptidase B-like (CPB-like) enzymes only cleave the basic residues lysine or arginine. There are nine members in the A/B family: CPA1 CPA2 CPA3 CPA4 CPA5 CPA6 CPB CPO and CPU. CPA1 CPA2 and CPB are produced by the pancreas. The A forms have slightly different specificities with CPA1 preferring aliphatic and small aromatic residues and CPA2 preferring the bulkier aromatic side chains. CPA3 is found in secretory granules of mast cells and functions in inflammatory processes. CPA4 detected in hormone-regulated tissues is thought to play a role in prostate cancer. CPA5 is present in discrete regions of pituitary and other tissues and cleaves aliphatic C-terminal residues. CPA6 is highly expressed in embryonic brain and optic muscle suggesting that it may play a specific role in cell migration and axonal guidance. CPU (also called CPB2) is produced and secreted by the liver as the inactive precursor PCPU commonly referred to as thrombin-activatable fibrinolysis inhibitor (TAFI). Little is known about CPO but it has been suggested to have specificity for acidic residues.
	gnl\|CDD\|133072	217	538	322	Gaps:28	100.00	294	41.16	15.65	2e-84	cd03860 M14_CP_A-B_like The Peptidase M14 Carboxypeptidase (CP) A/B subfamily is one of two main M14 CP subfamilies defined by sequence and structural homology the other being the N/E subfamily. CPs hydrolyze single C-terminal amino acids from polypeptide chains. They have a recognition site for the free C-terminal carboxyl group which is a key determinant of specificity. Enzymes belonging to the A/B subfamily are normally synthesized as inactive precursors containing preceding signal peptide followed by a globular N-terminal pro-region linked to the enzyme these proenzymes are called procarboxypeptidases. The A/B enzymes can be further divided based on their substrate specificity Carboxypeptidase A-like (CPA-like) enzymes favor hydrophobic residues while carboxypeptidase B-like (CPB-like) enzymes only cleave the basic residues lysine or arginine. There are nine members in the A/B family: CPA1 CPA2 CPA3 CPA4 CPA5 CPA6 CPB CPO and CPU. CPA1 CPA2 and CPB are produced by the pancreas. The A forms have slightly different specificities with CPA1 preferring aliphatic and small aromatic residues and CPA2 preferring the bulkier aromatic side chains. CPA3 is found in secretory granules of mast cells and functions in inflammatory processes. CPA4 is detected in hormone-regulated tissues and is thought to play a role in prostate cancer. CPA5 is present in discrete regions of pituitary and other tissues and cleaves aliphatic C-terminal residues. CPA6 is highly expressed in embryonic brain and optic muscle suggesting that it may play a specific role in cell migration and axonal guidance. CPU (also called CPB2) is produced and secreted by the liver as the inactive precursor PCPU commonly referred to as thrombin-activatable fibrinolysis inhibitor (TAFI). Little is known about CPO but it has been suggested to have specificity for acidic residues.
	gnl\|CDD\|128879	217	524	308	Gaps:31	100.00	277	45.85	17.33	1e-74	smart00631 Zn_pept Zn_pept.
	gnl\|CDD\|109310	226	530	305	Gaps:32	98.91	276	45.79	16.85	2e-69	pfam00246 Peptidase_M14 Zinc carboxypeptidase.
	gnl\|CDD\|133082	214	539	326	Gaps:28	99.33	300	37.25	18.79	8e-58	cd03871 M14_CPB Peptidase M14 Carboxypeptidase B (CPB) belongs to the carboxypeptidase A/B subfamily of the M14 family of metallocarboxypeptidases (MCPs). The M14 family are zinc-binding CPs which hydrolyze single C-terminal amino acids from polypeptide chains and have a recognition site for the free C-terminal carboxyl group which is a key determinant of specificity. Carboxypeptidase B (CPB) enzymes only cleave the basic residues lysine or arginine. A/B subfamily enzymes are normally synthesized as inactive precursors containing preceding signal peptide followed by a globular N-terminal pro-region linked to the enzyme these proenzymes are called procarboxypeptidases. The procarboxypeptidase B (PCPB) is produced by the exocrine pancreas and stored as stable zymogen in the pancreatic granules until secretion into the digestive tract occurs. PCPB has been reported to be a good serum marker for the diagnosis of acute pancreatitis and graft rejection in pancreas transplant recipients.
rpsblast_kog	gnl\|CDD\|37861	85	537	453	Gaps:53	98.09	418	34.88	19.51	8e-86	KOG2650 KOG2650 KOG2650 Zinc carboxypeptidase [Function unknown].
	gnl\|CDD\|37860	226	360	135	Gaps:8	25.40	500	29.92	17.32	7e-12	KOG2649 KOG2649 KOG2649 Zinc carboxypeptidase [General function prediction only].
	gnl\|CDD\|35481	4	59	56	Gaps:7	10.28	1605	70.91	9.70	0.087	KOG0260 KOG0260 KOG0260 RNA polymerase II large subunit [Transcription].
	gnl\|CDD\|38847	270	375	106	Gaps:37	18.77	650	32.79	13.11	0.23	KOG3641 KOG3641 KOG3641 Zinc carboxypeptidase [Amino acid transport and metabolism].
	gnl\|CDD\|37281	4	226	223	Gaps:2	10.44	661	27.54	13.04	0.69	KOG2070 KOG2070 KOG2070 Guanine nucleotide exchange factor [Nucleotide transport and metabolism].

Other analysis

Functional Gbrowse

Structural Gbrowse

Genome Mapping at gene locus

Analysis	Hit name	RefStart	RefEnd	Hit length	Hit identity	Hit coverage	eValue	Hit description
blastx_uniprot_trembl	Q0U307	387486	389245	539	70.62	98.52	n/a	Putative uncharacterized protein - Phaeosphaeria nodorum (Septoria nodorum)
	A7EUC0	387510	389206	596	55.70	91.28	n/a	Putative uncharacterized protein - Sclerotinia sclerotiorum (strain ATCC 18683 / 1980 / Ss-1) (White mold) (Whetzelinia sclerotiorum)
	A6RCF5	387486	389182	607	54.86	86.49	n/a	Putative uncharacterized protein - Ajellomyces capsulata (strain NAm1) (Histoplasma capsulatum)
	Q1E9E6	387522	389278	582	51.90	95.02	n/a	Putative uncharacterized protein - Coccidioides immitis
	Q0C9B4	387516	389245	586	54.70	90.78	n/a	Putative uncharacterized protein - Aspergillus terreus (strain NIH 2624)
	Q2TZK2	387660	389224	604	55.03	80.63	n/a	Zinc carboxypeptidase - Aspergillus oryzae
	A1DGH9	388010	389185	587	58.78	66.95	n/a	Zinc carboxypeptidase putative - Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / NRRL 181) (Aspergillus fischerianus (strain ATCC 1020 / DSM 3700 / NRRL 181))
	A1CSU3	388010	389182	590	59.38	65.08	n/a	Zinc carboxypeptidase putative - Aspergillus clavatus
	Q4X1U0	388010	389185	586	57.65	66.89	n/a	Zinc carboxypeptidase putative - Aspergillus fumigatus (Sartorya fumigata)
	A2QZA2	388010	389179	621	58.03	62.16	n/a	Contig An12c0110 complete genome. precursor - Aspergillus niger
	Q0U307	387486	389245	539	70.62	98.52	n/a	Putative uncharacterized protein - Phaeosphaeria nodorum (Septoria nodorum)
	A6RCF5	387486	389182	607	54.86	86.49	n/a	Putative uncharacterized protein - Ajellomyces capsulata (strain NAm1) (Histoplasma capsulatum)
blastx_FNG31_prot	SNU13857.1	387486	389245	536	67.99	98.51	n/a	Stagonospora nodorum hypothetical protein translation
	SS1G_08927.1	387510	389206	596	55.70	91.28	n/a	sclerotinia_sclerotiorum hypothetical protein translation 597 aa
	CIMG_00817.2	387522	389278	582	51.90	95.02	n/a	Coccidioides immitis hypothetical protein translation 583 aa
	AN6119	387498	389173	586	55.40	86.86	n/a	Aspergillus nidulans AN6119.3 conserved hypothetical protein translation 587 aa
	AO090011000821	387660	389224	604	55.03	80.63	n/a	Aspergillus oryzae zinc carboxypeptidase
	jgi:Triree:37546	387738	389188	547	56.07	82.82	n/a	Trichoderma reesei
	FG01898.1	387789	389188	563	55.84	77.62	n/a	fusarium_graminearum hypothetical protein 564 aa
	gi:71001064:ref:XP_755213.1:	388010	389185	586	57.65	66.89	n/a	Aspergillus fumigatus Af293 zinc carboxypeptidase
	BC1G_10920.1	387831	389095	540	60.25	73.15	n/a	Botrytis cinerea hypothetical protein translation 541 aa
	CHG07807.1	388019	389188	584	57.03	65.75	n/a	Chaetomium globosum hypothetical protein translation 585 aa
	SNU13857.1	387486	389245	536	67.99	98.51	n/a	Stagonospora nodorum hypothetical protein translation
blat_BES	no results
blastx_SN15proteins	gnl:WGS:AAGI:SNOT_13857.2:gb:EAT78881	387486	389245	539	70.62	98.52	n/a	n/a
	gnl:WGS:AAGI:SNOT_09739.2:gb:EAT83004	388196	389161	417	33.44	71.70	n/a	n/a
	gnl:WGS:AAGI:SNOT_13403.2:gb:EAT79287	388193	389137	440	36.54	68.41	n/a	n/a
	gnl:WGS:AAGI:SNOT_07428.2:gb:EAT84894	388196	389128	415	34.14	69.88	n/a	n/a
	gnl:WGS:AAGI:SNOT_11698.1:gb:EAT80742	388196	388951	441	35.02	58.28	n/a	n/a
	gnl:WGS:AAGI:SNOT_13857.2:gb:EAT78881	387486	389245	539	70.62	98.52	n/a	n/a
RepeatMasker_SR	no results
RepeatMasker_LC	no results
sim4_Lm_allest	RS0AAC13YL03FM1	389192	389395	204	100.00	100.00	n/a	Lm isolate M1 - V8 (5 days) and minimal medium (8 hours) mycelial library
sim4_lm_allest6_clctg	RS0AAC13YL03FM1	389192	389395	204	100.00	100.00	n/a	Lm isolate M1 - V8 (5 days) and minimal medium (8 hours) mycelial library
sim4_cDNA_BHowlett	no results
blastx_uniprot_sprot	P38836	388196	389176	430	46.77	72.09	n/a	Protein ECM14 precursor - Saccharomyces cerevisiae (Baker's yeast)
	O74818	388196	389173	497	40.45	63.18	n/a	Uncharacterized carboxypeptidase C337.07c precursor - Schizosaccharomyces pombe (Fission yeast)
	P00732	388187	389176	417	37.87	72.18	n/a	Carboxypeptidase B precursor - Bos taurus (Bovine)
	Q504N0	388271	389137	417	40.54	62.11	n/a	Carboxypeptidase A2 precursor - Mus musculus (Mouse)
	P19223	388181	389173	415	37.42	72.77	n/a	Carboxypeptidase B precursor - Rattus norvegicus (Rat)
	Q6P8K8	388208	389137	420	39.36	67.14	n/a	Carboxypeptidase A4 precursor - Mus musculus (Mouse)
	P48052	388184	389137	417	37.85	69.06	n/a	Carboxypeptidase A2 precursor - Homo sapiens (Human)
	Q2KIG3	388196	389185	423	37.70	72.10	n/a	Carboxypeptidase B2 precursor - Bos taurus (Bovine)
	P04069	388208	389173	303	38.23	96.70	n/a	Carboxypeptidase B - Astacus fluviatilis (Broad-fingered crayfish) (Astacus astacus)
	Q9UI42	388208	389137	421	40.07	66.98	n/a	Carboxypeptidase A4 precursor - Homo sapiens (Human)
REPET	no results
MUMer_show_coords_lm_WA74	no results