GRS - Genome Report System

Leptosphaeria maculans GRS

Functional annotation

Lema_P081800.1

Gene Identifier

DbXrefs

Domain/Motif (Interproscan results)

Localization / Targeting

Predicted function

Blast based analysis

Analysis	Hit	start	end	length	Note	Hit coverage	Hit length	Hit pident	Hit pcons	eValue	Hit description
blastp_kegg	nfi:NFIA_064360	50	465	416	n/a	77.16	521	45.02	19.90	1e-110	cytochrome P450 monooxigenase GliC2
	afm:AFUA_3G12960	50	465	416	n/a	72.73	550	46.50	19.00	1e-109	cytochrome P450 monooxigenase GliC-like (EC:1.14.-.-) K00517
	afm:AFUA_6G09670	50	450	401	n/a	69.34	512	39.44	23.38	2e-76	cytochrome P450 oxidoreductase GliC (EC:1.14.-.-) K00517
	nfi:NFIA_055360	50	440	391	n/a	69.79	513	38.83	23.74	4e-76	Cytochrome P450 oxidoreductase putative
	bfu:BC1G_07447	81	438	358	n/a	69.16	509	38.07	22.44	2e-71	hypothetical protein
	ang:An09g00560	47	438	392	n/a	68.71	521	36.59	24.30	3e-70	hypothetical protein
	aor:AO090001000041	81	462	382	n/a	66.46	486	39.01	25.70	5e-67	hypothetical protein
	nfi:NFIA_074290	57	437	381	n/a	63.01	519	35.78	23.24	4e-56	Cytochrome P450 oxidoreductase putative
	mgr:MGG_07743	81	433	353	n/a	52.64	549	40.48	22.84	1e-53	hypothetical protein
	aor:AO090003001544	126	440	315	n/a	82.83	367	36.51	21.38	3e-50	hypothetical protein
blastp_uniprot_sprot	sp\|Q4G0S4\|C27C1_HUMAN	296	436	141	n/a	27.15	372	40.59	20.79	1e-13	Cytochrome P450 27C1 OS Homo sapiens GN CYP27C1 PE 2 SV 1
	sp\|Q91Z85\|CP17A_PERLE	294	433	140	n/a	24.56	509	32.80	26.40	3e-12	Steroid 17-alpha-hydroxylase/17 20 lyase OS Peromyscus leucopus GN Cyp17a1 PE 3 SV 1
	sp\|Q7Z449\|CP2U1_HUMAN	301	443	143	n/a	22.43	544	36.07	20.49	6e-12	Cytochrome P450 2U1 OS Homo sapiens GN CYP2U1 PE 1 SV 1
	sp\|P29981\|CP4C1_BLADI	81	436	356	n/a	23.87	511	37.70	18.85	1e-11	Cytochrome P450 4C1 OS Blaberus discoidalis GN CYP4C1 PE 2 SV 1
	sp\|P27786\|CP17A_MOUSE	301	441	141	n/a	24.26	507	32.52	26.02	1e-11	Steroid 17-alpha-hydroxylase/17 20 lyase OS Mus musculus GN Cyp17a1 PE 2 SV 1
	sp\|P11715\|CP17A_RAT	301	441	141	n/a	24.26	507	33.33	24.39	3e-11	Steroid 17-alpha-hydroxylase/17 20 lyase OS Rattus norvegicus GN Cyp17a1 PE 1 SV 2
	sp\|Q54SV9\|C515B_DICDI	296	435	140	n/a	25.96	493	32.81	22.66	3e-11	Probable cytochrome P450 515B1 OS Dictyostelium discoideum GN cyp515b1 PE 2 SV 1
	sp\|P05093\|CP17A_HUMAN	299	440	142	n/a	25.00	508	34.65	22.05	4e-11	Steroid 17-alpha-hydroxylase/17 20 lyase OS Homo sapiens GN CYP17A1 PE 1 SV 1
	sp\|Q556M5\|C5081_DICDI	301	436	136	n/a	24.79	484	33.33	24.17	4e-11	Probable cytochrome P450 508A1 OS Dictyostelium discoideum GN cyp508a1-1 PE 3 SV 1
	sp\|Q8HYN0\|CP17A_PAPCY	299	440	142	n/a	25.00	508	34.65	21.26	5e-11	Steroid 17-alpha-hydroxylase/17 20 lyase OS Papio cynocephalus GN CYP17A1 PE 2 SV 1
blastp_pdb	no results
rpsblast_cdd	gnl\|CDD\|109135	81	438	358	Gaps:59	87.80	459	22.58	15.14	4e-29	pfam00067 p450 Cytochrome P450. Cytochrome P450s are haem-thiolate proteins involved in the oxidative degradation of various compounds. They are particularly well known for their role in the degradation of environmental toxins and mutagens. They can be divided into 4 classes according to the method by which electrons from NAD(P)H are delivered to the catalytic site. Sequence conservation is relatively low within the family - there are only 3 absolutely conserved residues - but their general topography and structural fold are highly conserved. The conserved core is composed of a coil termed the 'meander' a four-helix bundle helices J and K and two sets of beta-sheets. These constitute the haem-binding loop (with an absolutely conserved cysteine that serves as the 5th ligand for the haem iron) the proton-transfer groove and the absolutely conserved EXXR motif in helix K. While prokaryotic P450s are soluble proteins most eukaryotic P450s are associated with microsomal membranes. their general enzymatic function is to catalyse regiospecific and stereospecific oxidation of non-activated hydrocarbons at physiological temperatures.
	gnl\|CDD\|32307	76	440	365	Gaps:74	84.91	411	24.36	14.61	6e-22	COG2124 CypX Cytochrome P450 [Secondary metabolites biosynthesis transport and catabolism].
	gnl\|CDD\|129774	266	303	38	n/a	5.56	683	28.95	21.05	3.1	TIGR00691 spoT_relA (p)ppGpp is a regulatory metabolite of the stringent response but appears also to be involved in antibiotic biosynthesis in some species.
	gnl\|CDD\|132465	163	200	38	Gaps:6	22.22	198	34.09	13.64	4.1	TIGR03424 urea_degr_1 A number of bacteria degrade urea as a nitrogen source by the urea carboxylase/allophanate hydrolase pathway which uses biotin and consumes ATP rather than my means of the nickel-dependent enzyme urease. This model represents one of a pair of homologous tandem uncharacterized genes found together with the urea carboxylase and allophanate hydrolase genes.
	gnl\|CDD\|137449	115	195	81	Gaps:13	36.17	188	23.53	19.12	4.4	PRK09640 PRK09640 RNA polymerase sigma factor SigX Reviewed.
rpsblast_kog	gnl\|CDD\|35379	29	441	413	Gaps:66	92.35	497	20.92	16.99	1e-34	KOG0157 KOG0157 KOG0157 Cytochrome P450 CYP4/CYP19/CYP26 subfamilies [Secondary metabolites biosynthesis transport and catabolism Lipid transport and metabolism].
	gnl\|CDD\|35380	20	437	418	Gaps:88	93.39	499	20.60	14.59	4e-23	KOG0158 KOG0158 KOG0158 Cytochrome P450 CYP3/CYP5/CYP6/CYP9 subfamilies [Secondary metabolites biosynthesis transport and catabolism].
	gnl\|CDD\|35381	79	434	356	Gaps:72	77.84	519	22.77	16.58	1e-21	KOG0159 KOG0159 KOG0159 Cytochrome P450 CYP11/CYP12/CYP24/CYP27 subfamilies [Secondary metabolites biosynthesis transport and catabolism].
	gnl\|CDD\|35378	20	437	418	Gaps:79	93.87	489	20.92	13.07	9e-20	KOG0156 KOG0156 KOG0156 Cytochrome P450 CYP2 subfamily [Secondary metabolites biosynthesis transport and catabolism].
	gnl\|CDD\|35903	268	468	201	Gaps:57	51.44	486	22.80	11.20	3e-08	KOG0684 KOG0684 KOG0684 Cytochrome P450 [Secondary metabolites biosynthesis transport and catabolism].

Other analysis

Functional Gbrowse

Structural Gbrowse

Genome Mapping at gene locus

Analysis	Hit name	RefStart	RefEnd	Hit length	Hit identity	Hit coverage	eValue	Hit description
blastx_uniprot_trembl	Q6Q881	1389164	1391037	470	99.79	100.00	n/a	SirC - Leptosphaeria maculans (Blackleg fungus)
	Q4WYG6	1389329	1391016	550	44.49	85.82	n/a	Cytochrome P450 monooxigenase GliC-like putative - Aspergillus fumigatus (Sartorya fumigata)
	A1D6C8	1389329	1391016	521	43.28	90.02	n/a	Cytochrome P450 monooxigenase GliC2 - Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / NRRL 181) (Aspergillus fischerianus (strain ATCC 1020 / DSM 3700 / NRRL 181))
	Q0CD04	1389236	1390475	403	42.43	91.81	n/a	Predicted protein - Aspergillus terreus (strain NIH 2624)
	A1CGH0	1389248	1391016	500	35.74	96.80	n/a	Cytochrome P450 monooxygenase putative - Aspergillus clavatus
	A6S4D2	1389320	1391007	509	37.93	91.16	n/a	Putative uncharacterized protein - Botryotinia fuckeliana (strain B05.10) (Noble rot fungus) (Botrytis cinerea)
	A2QT28	1389287	1390472	521	35.33	67.37	n/a	Contig An09c0030 complete genome. precursor - Aspergillus niger
	A9Q1E3	1389341	1391013	501	42.42	90.82	n/a	Cytochrome P450 monooxigenase - Trichoderma virens
	A4QYQ1	1389389	1390998	549	35.57	81.42	n/a	Putative uncharacterized protein - Magnaporthe grisea (Rice blast fungus) (Pyricularia grisea)
	Q2UPB1	1389329	1390472	486	34.23	68.52	n/a	Predicted protein - Aspergillus oryzae
	Q6Q881	1389164	1391037	470	99.79	100.00	n/a	SirC - Leptosphaeria maculans (Blackleg fungus)
blastx_FNG31_prot	gi:70999207:ref:XP_754325.1:	1389329	1391016	550	44.49	85.82	n/a	Aspergillus fumigatus Af293 cytochrome P450
	BC1G_07447.1	1389320	1391007	509	37.93	91.16	n/a	Botrytis cinerea hypothetical protein translation 510 aa
	MGG_07743.5	1389389	1390998	549	35.57	81.42	n/a	magnaporthe_grisea hypothetical protein translation 550 aa
	AO090001000041	1389329	1390472	486	34.23	68.52	n/a	Aspergillus oryzae predicted protein
	AO090003001544	1389656	1391010	367	37.47	98.91	n/a	Aspergillus oryzae predicted protein
	SS1G_08565.1	1389851	1391007	338	37.86	91.42	n/a	sclerotinia_sclerotiorum hypothetical protein translation 339 aa
	gi:70992015:ref:XP_750856.1:	1389308	1391010	530	35.70	87.74	n/a	Aspergillus fumigatus Af293 cytochrome P450
	jgi:Triree:38442	1389560	1390204	413	34.47	49.88	n/a	Trichoderma reesei
	AN8510	1390248	1390986	324	43.98	58.95	n/a	Aspergillus nidulans AN8510.3 conserved hypothetical protein translation 325 aa
	SNU13040.1	1389329	1390090	471	31.90	49.26	n/a	Stagonospora nodorum hypothetical protein translation
blat_BES	QS0AAD27ZD07RM1	1389442	1390142	839	n/a	n/a	n/a	n/a
	QS0AAD8ZE07FM1	1389304	1389978	741	n/a	n/a	n/a	n/a
	QS0AAE126ZF08RM1	1390246	1390887	787	n/a	n/a	n/a	n/a
blastx_SN15proteins	gnl:WGS:AAGI:SNOT_13040.1:gb:EAT79367	1389329	1390090	471	31.90	49.26	n/a	n/a
RepeatMasker_SR	no results
RepeatMasker_LC	no results
sim4_Lm_allest	EB763228	1389182	1389870	572	99.65	100.00	n/a	Lm isolate PL86 - V8 mycelial library (from EMBL)
	EB763425	1389161	1389868	589	99.66	100.00	n/a	Lm isolate PL86 - V8 mycelial library (from EMBL)
	EB763046	1389205	1389834	514	99.22	100.00	n/a	Lm isolate PL86 - V8 mycelial library (from EMBL)
	EB763425	1389161	1389868	589	99.66	100.00	n/a	Lm isolate PL86 - V8 mycelial library (from EMBL)
	EB763236	1390405	1391116	549	99.45	100.00	n/a	Lm isolate PL86 - V8 mycelial library (from EMBL)
	EB763433	1390419	1391082	501	99.00	100.00	n/a	Lm isolate PL86 - V8 mycelial library (from EMBL)
sim4_lm_allest6_clctg	CL_lm_SuperContig_13_v2_lmctg_1147_v2_002.Contig1_v6	1389161	1389870	593	99.66	100.00	n/a	n/a
	CL_lm_SuperContig_13_v2_lmctg_1147_v2_002.Contig1_v6	1389161	1389870	593	99.66	100.00	n/a	n/a
	CL_lm_SuperContig_13_v2_lmctg_1147_v2_003.Contig1_v6	1390405	1391116	549	99.45	100.00	n/a	n/a
sim4_cDNA_BHowlett	no results
blastx_uniprot_sprot	no results
REPET	no results
MUMer_show_coords_lm_WA74	no results