GRS - Genome Report System

Leptosphaeria maculans GRS

Functional annotation

Lema_P062070.1

Gene Identifier

DbXrefs

Domain/Motif (Interproscan results)

Localization / Targeting

Predicted function

Blast based analysis

Analysis	Hit	start	end	length	Note	Hit coverage	Hit length	Hit pident	Hit pcons	eValue	Hit description
blastp_kegg	ang:An16g04400	134	253	120	n/a	98.36	122	55.83	19.17	1e-40	hypothetical protein
	ani:AN4942.2	134	256	123	n/a	87.20	125	61.47	16.51	2e-34	similar to nuclear transport factor 2
	cnb:CNBL2410	134	254	121	n/a	95.16	124	57.63	17.80	7e-34	hypothetical protein
	cne:CNH02390	134	254	121	n/a	95.16	124	57.63	17.80	7e-34	nuclear transport factor 2 (ntf-2)
	pan:PODANSg3006	136	254	119	n/a	96.77	124	56.67	15.00	2e-33	hypothetical protein
	ath:AT1G27310	136	254	119	n/a	98.36	122	54.17	13.33	1e-30	NTF2A NTF2A (NUCLEAR TRANSPORT FACTOR 2A) Ran GTPase binding / protein transporter
	vvi:100252245	136	254	119	n/a	94.31	123	58.62	12.93	4e-28	GSVIVT00002791001 hypothetical protein LOC100252245
	pop:POPTR_548036	136	254	119	n/a	94.31	123	56.90	12.93	1e-26	hypothetical protein
	pop:POPTR_554771	136	254	119	n/a	98.37	123	53.72	12.40	3e-26	hypothetical protein
	nfi:NFIA_066820	134	256	123	n/a	100.00	124	62.90	16.13	3e-26	nuclear transport factor NTF-2 putative
blastp_uniprot_sprot	sp\|Q96VN3\|NTF2_EMENI	134	256	123	n/a	87.20	125	61.47	16.51	2e-35	Nuclear transport factor 2 OS Emericella nidulans GN ntf2 PE 3 SV 1
	sp\|P87102\|NTF2_NEUCR	136	253	118	n/a	95.97	124	52.94	17.65	2e-32	Nuclear transport factor 2 OS Neurospora crassa GN ntf-2 PE 2 SV 1
	sp\|Q9C7F5\|NTF2_ARATH	136	254	119	n/a	88.89	126	58.04	14.29	3e-28	Nuclear transport factor 2 OS Arabidopsis thaliana GN NTF2 PE 2 SV 1
	sp\|Q8NJ52\|NTF2_CLAHE	134	257	124	n/a	100.00	125	68.80	11.20	3e-28	Nuclear transport factor 2 OS Cladosporium herbarum GN NTF2 PE 1 SV 1
	sp\|Q10100\|NTF2_SCHPO	134	226	93	n/a	75.61	123	52.69	21.51	2e-26	Nuclear transport factor 2 OS Schizosaccharomyces pombe GN ntf2 PE 2 SV 2
	sp\|Q86HW7\|NTF2_DICDI	140	253	114	n/a	90.55	127	44.35	21.74	3e-24	Nuclear transport factor 2 OS Dictyostelium discoideum GN nutf2 PE 1 SV 1
	sp\|Q6FRC6\|NTF2_CANGA	136	221	86	n/a	68.80	125	53.49	20.93	5e-24	Nuclear transport factor 2 OS Candida glabrata GN NTF2 PE 3 SV 1
	sp\|Q75AA5\|NTF2_ASHGO	136	221	86	n/a	68.80	125	53.49	20.93	2e-23	Nuclear transport factor 2 OS Ashbya gossypii GN NTF2 PE 3 SV 1
	sp\|P33331\|NTF2_YEAST	136	226	91	n/a	72.80	125	50.55	19.78	5e-23	Nuclear transport factor 2 OS Saccharomyces cerevisiae GN NTF2 PE 1 SV 2
	sp\|Q6CQX4\|NTF2_KLULA	136	226	91	n/a	72.80	125	50.55	20.88	5e-23	Nuclear transport factor 2 OS Kluyveromyces lactis GN NTF2 PE 3 SV 1
blastp_pdb	1gyb_D	136	226	91	n/a	72.80	125	50.55	18.68	3e-23	mol:protein length:125 NUCLEAR TRANSPORT FACTOR 2
	1gyb_C	136	226	91	n/a	72.80	125	50.55	18.68	3e-23	mol:protein length:125 NUCLEAR TRANSPORT FACTOR 2
	1gyb_B	136	226	91	n/a	72.80	125	50.55	18.68	3e-23	mol:protein length:125 NUCLEAR TRANSPORT FACTOR 2
	1gyb_A	136	226	91	n/a	72.80	125	50.55	18.68	3e-23	mol:protein length:125 NUCLEAR TRANSPORT FACTOR 2
	1gy7_D	136	226	91	n/a	72.80	125	50.55	18.68	3e-23	mol:protein length:125 NUCLEAR TRANSPORT FACTOR 2
	1gy7_C	136	226	91	n/a	72.80	125	50.55	18.68	3e-23	mol:protein length:125 NUCLEAR TRANSPORT FACTOR 2
	1gy7_B	136	226	91	n/a	72.80	125	50.55	18.68	3e-23	mol:protein length:125 NUCLEAR TRANSPORT FACTOR 2
	1gy7_A	136	226	91	n/a	72.80	125	50.55	18.68	3e-23	mol:protein length:125 NUCLEAR TRANSPORT FACTOR 2
	1zo2_B	137	252	116	n/a	75.97	129	47.96	15.31	1e-19	mol:protein length:129 nuclear transport factor 2
	1zo2_A	137	252	116	n/a	75.97	129	47.96	15.31	1e-19	mol:protein length:129 nuclear transport factor 2
rpsblast_cdd	gnl\|CDD\|29630	136	254	119	Gaps:2	100.00	119	47.06	17.65	6e-34	cd00780 NTF2 Nuclear transport factor 2 (NTF2) domain plays an important role in the trafficking of macromolecules ions and small molecules between the cytoplasm and nucleus. This bi-directional transport of macromolecules across the nuclear envelope requires many soluble factors that includes GDP-binding protein Ran (RanGDP). RanGDP is required for both import and export of proteins and poly(A) RNA. RanGDP also has been implicated in cell cycle control specifically in mitotic spindle assembly. In interphase cells RanGDP is predominately nuclear and thought to be GTP bound but it is also present in the cytoplasm probably in the GDP-bound state. NTF2 mediates the nuclear import of RanGDP. NTF2 binds to both RanGDP and FxFG repeat-containing nucleoporins..
	gnl\|CDD\|111071	140	252	113	Gaps:6	100.00	119	41.18	13.45	1e-27	pfam02136 NTF2 Nuclear transport factor 2 (NTF2) domain. This family includes the NTF2-like Delta-5-3-ketosteroid isomerase proteins.
	gnl\|CDD\|29628	141	253	113	Gaps:11	100.00	124	29.84	13.71	3e-11	cd00531 NTF2_like Nuclear transport factor 2 (NTF2-like) superfamily. This family includes members of the NTF2 family Delta-5-3-ketosteroid isomerases Scytalone Dehydratases and the beta subunit of Ring hydroxylating dioxygenases. This family is a classic example of divergent evolution wherein the proteins have many common structural details but diverge greatly in their function. For example nuclear transport factor 2 (NTF2) mediates the nuclear import of RanGDP and binds to both RanGDP and FxFG repeat-containing nucleoporins while Ketosteroid isomerases catalyze the isomerization of delta-5-3-ketosteroid to delta-4-3-ketosteroid by intramolecular transfer of the C4-beta proton to the C6-beta position. While the function of the beta sub-unit of the Ring hydroxylating dioxygenases is not known Scytalone Dehydratases catalyzes two reactions in the biosynthetic pathway that produces fungal melanin. Members of the NTF2-like superfamily are widely distributed among bacteria archaea and eukaryotes..
	gnl\|CDD\|110776	41	73	33	n/a	8.73	378	36.36	3.03	3.6	pfam01803 LIM_bind LIM-domain binding protein. The LIM-domain binding protein binds to the LIM domain pfam00412 of LIM homeodomain proteins which are transcriptional regulators of development. Nuclear LIM interactor (NLI) / LIM domain-binding protein 1 (LDB1) is located in the nuclei of neuronal cells during development it is co-expressed with Isl1 in early motor neuron differentiation and has a suggested role in the Isl1 dependent development of motor neurons. It is suggested that these proteins act synergistically to enhance transcriptional efficiency by acting as co-factors for LIM homeodomain and Otx class transcription factors both of which have essential roles in development. The Drosophila protein Chip is required for segmentation and activity of a remote wing margin enhancer. Chip is a ubiquitous chromosomal factor required for normal expression of diverse genes at many stages of development. It is suggested that Chip cooperates with different LIM domain proteins and other factors to structurally support remote enhancer-promoter interactions.
	gnl\|CDD\|131355	34	72	39	n/a	4.58	851	33.33	12.82	4.5	TIGR02302 aProt_lowcomp Members of this family are long (~850 residue) bacterial proteins from the alpha Proteobacteria. Each has 2-3 predicted transmembrane helices near the N-terminus and a long C-terminal region that includes stretches of Gln/Gly-rich low complexity sequence predicted by TMHMM to be outside the membrane. In Bradyrhizobium japonicum two tandem reading frames are together homologous the single members found in other species the cutoffs scores are set low enough that the longer scores above the trusted cutoff and the shorter above the noise cutoff for this model.
rpsblast_kog	gnl\|CDD\|37315	136	254	119	Gaps:2	94.44	126	52.10	15.97	6e-37	KOG2104 KOG2104 KOG2104 Nuclear transport factor 2 [Intracellular trafficking secretion and vesicular transport].
	gnl\|CDD\|35339	138	253	116	Gaps:8	28.64	419	33.33	18.33	4e-13	KOG0116 KOG0116 KOG0116 RasGAP SH3 binding protein rasputin contains NTF2 and RRM domains [Signal transduction mechanisms].
	gnl\|CDD\|39554	141	253	113	Gaps:13	86.33	139	27.50	18.33	3e-12	KOG4353 KOG4353 KOG4353 RNA export factor NXT1 [RNA processing and modification].
	gnl\|CDD\|36837	17	81	65	Gaps:5	23.45	290	22.06	17.65	0.46	KOG1624 KOG1624 KOG1624 Mitochondrial/chloroplast ribosomal protein L4 [Translation ribosomal structure and biogenesis].
	gnl\|CDD\|35413	116	138	23	n/a	6.35	362	47.83	13.04	3.4	KOG0192 KOG0192 KOG0192 Tyrosine kinase specific for activated (GTP-bound) p21cdc42Hs [Signal transduction mechanisms].

Other analysis

Functional Gbrowse

Structural Gbrowse

Genome Mapping at gene locus

Analysis	Hit name	RefStart	RefEnd	Hit length	Hit identity	Hit coverage	eValue	Hit description
blastx_uniprot_trembl	Q0UJS5	1706563	1707066	124	75.00	93.55	n/a	Putative uncharacterized protein - Phaeosphaeria nodorum (Septoria nodorum)
blastx_uniprot_trembl	Q8NKB7	1706563	1706929	124	80.90	71.77	n/a	Putative nuclear transport factor 2 - Alternaria alternata (Alternaria rot fungus)
blastx_FNG31_prot	AN4942	1706563	1707063	125	67.54	91.20	n/a	Aspergillus nidulans AN4942.3 nuclear transport factor 2 translation 126 aa
blat_BES	QS0AAD38ZB07FM1	1706189	1706542	806	n/a	n/a	n/a	n/a
	QS0AAD68ZB06RM1	1705428	1706194	1378	n/a	n/a	n/a	n/a
	QS0AAD65ZG09RM1	1706189	1707145	1408	n/a	n/a	n/a	n/a
	QS0AAD14ZF05RM1	1706923	1707393	808	n/a	n/a	n/a	n/a
	QS0AAD22ZH11RM1	1706923	1707408	745	n/a	n/a	n/a	n/a
blastx_SN15proteins	gnl:WGS:AAGI:SNOT_07989.2:gb:EAT84265	1706563	1707066	124	75.00	93.55	n/a	n/a
RepeatMasker_SR	no results
RepeatMasker_LC	no results
sim4_Lm_allest	RS0AAE12YK08CM1	1705812	1706146	154	100.00	100.00	n/a	Lm isolate JN3 - Conidia germinating in liquid Fries for 48 h (insert up to 1kb)
	RS0AAF2YB11CM1	1705899	1706025	127	100.00	100.00	n/a	Lm isolate JN3 - Conidia germinating in liquid Fries for 48 h (insert 1 to 2kb)
	RS0ABB7YP17CM1	1705814	1706146	152	100.00	100.00	n/a	Lm isolate JN3 - cotyledon-pathogen interaction 13 dpi (insert 1.2 to 2kb)
	RS0AAB26YD04FM1	1706324	1707292	676	99.56	100.00	n/a	Lm isolate M1 - mycelia grown for 5 days and 8 hours on oilseed rape juice medium
	RS0AAB22YE09FM1	1706314	1707281	674	99.85	100.00	n/a	Lm isolate M1 - mycelia grown for 5 days and 8 hours on oilseed rape juice medium
	RS0AAB4YE15FM1	1706873	1707275	353	100.00	100.00	n/a	Lm isolate M1 - mycelia grown for 5 days and 8 hours on oilseed rape juice medium
	RS0ABA10YJ12CM1	1706566	1707276	312	99.68	100.00	n/a	Lm isolate JN3 - cotyledon-pathogen interaction 13 dpi (insert 0.8 to 1.2kb)
sim4_lm_allest6_clctg	CL_lm_SuperContig_8_v2_lmctg_0854_v2_008.Contig1_v6	1704595	1706146	1273	100.00	100.00	n/a	n/a
	RS0AAF2YB11CM1	1705899	1706025	127	100.00	100.00	n/a	Lm isolate JN3 - Conidia germinating in liquid Fries for 48 h (insert 1 to 2kb)
	CL_lm_SuperContig_8_v2_lmctg_0854_v2_009.Contig1_v6	1706314	1707292	686	99.71	100.00	n/a	n/a
sim4_cDNA_BHowlett	no results
blastx_uniprot_sprot	Q96VN3	1706563	1707063	125	67.54	91.20	n/a	Nuclear transport factor 2 - Emericella nidulans (Aspergillus nidulans)
REPET	no results
MUMer_show_coords_lm_WA74	no results