GRS - Genome Report System

Leptosphaeria maculans GRS

Functional annotation

Lema_P039240.1

Gene Identifier

DbXrefs

Domain/Motif (Interproscan results)

Localization / Targeting

Predicted function

Blast based analysis

Analysis	Hit	start	end	length	Note	Hit coverage	Hit length	Hit pident	Hit pcons	eValue	Hit description
blastp_kegg	ssl:SS1G_07320	1	173	173	n/a	91.53	189	88.44	6.36	1e-104	GTP-binding protein SARA
	bfu:BC1G_15213	1	173	173	n/a	91.53	189	88.44	6.36	1e-104	GTP-binding protein sarA
	aor:AO090003000842	1	173	173	n/a	91.53	189	89.02	4.62	1e-103	vesicle coat complex COPII GTPase subunit SAR1 K07977 Arf/Sar family other
	ncr:NCU11181	1	173	173	n/a	79.36	218	86.71	8.67	1e-103	hypothetical protein K07977 Arf/Sar family other
	ang:An01g04040	1	173	173	n/a	91.53	189	88.44	5.20	1e-103	sarA secretion-associated GTP-binding protein sarA-Aspergillus niger K07977 Arf/Sar family other
	nfi:NFIA_019790	1	173	173	n/a	91.53	189	88.44	4.62	1e-102	small monomeric GTPase SarA putative
	afm:AFUA_1G04940	1	173	173	n/a	91.53	189	88.44	4.62	1e-102	small monomeric GTPase SarA K07977 Arf/Sar family other
	pan:PODANSg4840	1	173	173	n/a	91.53	189	85.55	9.25	1e-102	hypothetical protein
	mgr:MGG_06362	1	173	173	n/a	91.53	189	86.13	8.67	1e-102	GTP-binding protein SAR1 K07977 Arf/Sar family other
	ani:AN0411.2	1	173	173	n/a	91.53	189	87.28	5.20	1e-101	SARA_ASPNG GTP-binding protein SARA K07977 Arf/Sar family other
blastp_uniprot_sprot	sp\|Q0UKC0\|SAR1_PHANO	1	173	173	n/a	93.51	185	95.95	2.31	1e-111	Small COPII coat GTPase SAR1 OS Phaeosphaeria nodorum GN SAR1 PE 3 SV 2
	sp\|P0C583\|SAR1_NEUCR	1	173	173	n/a	91.53	189	86.71	8.67	1e-104	Small COPII coat GTPase sar-1 OS Neurospora crassa GN sar-1 PE 3 SV 1
	sp\|Q877B9\|SAR1_ASPOR	1	173	173	n/a	91.53	189	89.02	4.62	1e-104	Small COPII coat GTPase sar1 OS Aspergillus oryzae GN sar1 PE 3 SV 1
	sp\|P0C951\|SAR1_ASPNC	1	173	173	n/a	91.53	189	88.44	5.20	1e-104	Small COPII coat GTPase SAR1 OS Aspergillus niger (strain CBS 513.88 / FGSC A1513) GN sar1 PE 3 SV 1
	sp\|A1CRG9\|SAR1_ASPCL	1	173	173	n/a	91.53	189	88.44	4.62	1e-103	Small COPII coat GTPase sar1 OS Aspergillus clavatus GN sar1 PE 3 SV 1
	sp\|A1D4D1\|SAR1_NEOFI	1	173	173	n/a	91.53	189	88.44	4.62	1e-103	Small COPII coat GTPase sar1 OS Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181) GN sar1 PE 3 SV 1
	sp\|Q4WJS7\|SAR1_ASPFU	1	173	173	n/a	91.53	189	88.44	4.62	1e-103	Small COPII coat GTPase sar1 OS Aspergillus fumigatus GN sar1 PE 3 SV 1
	sp\|Q5EMZ6\|SAR1_MAGGR	1	173	173	n/a	91.53	189	86.13	8.67	1e-103	Small COPII coat GTPase SAR1 OS Magnaporthe grisea GN SAR1 PE 2 SV 1
	sp\|Q0CUN7\|SAR1_ASPTN	1	173	173	n/a	91.53	189	87.86	5.78	1e-103	Small COPII coat GTPase sar1 OS Aspergillus terreus (strain NIH 2624 / FGSC A1156) GN sar1 PE 3 SV 1
	sp\|Q5BGB9\|SAR1_EMENI	1	173	173	n/a	91.53	189	87.28	5.20	1e-102	Small COPII coat GTPase sar1 OS Emericella nidulans GN sar1 PE 3 SV 1
blastp_pdb	1m2o_D	2	173	172	n/a	89.47	190	77.06	8.82	2e-82	mol:protein length:190 GTP-binding protein SAR1
	1m2o_B	2	173	172	n/a	89.47	190	77.06	8.82	2e-82	mol:protein length:190 GTP-binding protein SAR1
	2qtv_B	4	172	169	n/a	100.00	167	77.25	8.38	2e-80	mol:protein length:167 Small COPII coat GTPase SAR1
	1f6b_B	3	173	171	n/a	81.31	198	65.22	15.53	1e-66	mol:protein length:198 SAR1
	1f6b_A	3	173	171	n/a	81.31	198	65.22	15.53	1e-66	mol:protein length:198 SAR1
	2fmx_B	3	173	171	n/a	82.56	195	65.22	15.53	1e-66	mol:protein length:195 GTP-binding protein SAR1b
	2fmx_A	3	173	171	n/a	82.56	195	65.22	15.53	1e-66	mol:protein length:195 GTP-binding protein SAR1b
	2gao_B	3	173	171	n/a	77.40	208	65.84	13.66	2e-66	mol:protein length:208 GTP-binding protein SAR1a
	2gao_A	3	173	171	n/a	77.40	208	65.84	13.66	2e-66	mol:protein length:208 GTP-binding protein SAR1a
	2fa9_B	3	173	171	n/a	85.19	189	64.60	15.53	3e-65	mol:protein length:189 GTP-binding protein SAR1b
rpsblast_cdd	gnl\|CDD\|133255	2	173	172	Gaps:2	91.58	190	73.56	9.77	1e-90	cd00879 Sar1 Sar1 subfamily. Sar1 is an essential component of COPII vesicle coats involved in export of cargo from the ER. The GTPase activity of Sar1 functions as a molecular switch to control protein-protein and protein-lipid interactions that direct vesicle budding from the ER. Activation of the GDP to the GTP-bound form of Sar1 involves the membrane-associated guanine nucleotide exchange factor (GEF) Sec12. Sar1 is unlike all Ras superfamily GTPases that use either myristoyl or prenyl groups to direct membrane association and function in that Sar1 lacks such modification. Instead Sar1 contains a unique nine-amino-acid N-terminal extension. This extension contains an evolutionarily conserved cluster of bulky hydrophobic amino acids referred to as the Sar1-N-terminal activation recruitment (STAR) motif. The STAR motif mediates the recruitment of Sar1 to ER membranes and facilitates its interaction with mammalian Sec12 GEF leading to activation.
	gnl\|CDD\|128475	2	173	172	Gaps:2	92.39	184	75.29	9.41	1e-85	smart00178 SAR Sar1p-like members of the Ras-family of small GTPases. Yeast SAR1 is an essential gene required for transport of secretory proteins from the endoplasmic reticulum to the Golgi apparatus.
	gnl\|CDD\|109094	2	173	172	Gaps:9	100.00	163	45.40	17.79	1e-65	pfam00025 Arf ADP-ribosylation factor family. Pfam combines a number of different Prosite families together.
	gnl\|CDD\|133254	6	172	167	Gaps:9	100.00	158	42.41	15.19	9e-44	cd00878 Arf_Arl Arf (ADP-ribosylation factor)/Arl (Arf-like) small GTPases. Arf proteins are activators of phospholipase D isoforms. Unlike Ras proteins they lack cysteine residues at their C-termini and therefore are unlikely to be prenylated. Arfs are N-terminally myristoylated. Members of the Arf family are regulators of vesicle formation in intracellular traffic that interact reversibly with membranes of the secretory and endocytic compartments in a GTP-dependent manner. They depart from other small GTP-binding proteins by a unique structural device interswitch toggle that implements front-back communication from N-terminus to the nucleotide binding site. Arf-like (Arl) proteins are close relatives of the Arf but only Arl1 has been shown to function in membrane traffic like the Arf proteins. Arl2 has an unrelated function in the folding of native tubulin and Arl4 may function in the nucleus. Most other Arf family proteins are so far relatively poorly characterized. Thus despite their significant sequence homologies Arf family proteins may regulate unrelated functions.
	gnl\|CDD\|133351	6	171	166	Gaps:9	99.37	158	37.58	18.47	4e-29	cd04151 Arl1 Arl1 subfamily. Arl1 (Arf-like 1) localizes to the Golgi complex where it is believed to recruit effector proteins to the trans-Golgi network. Like most members of the Arf family Arl1 is myristoylated at its N-terminal helix and mutation of the myristoylation site disrupts Golgi targeting. In humans the Golgi-localized proteins golgin-97 and golgin-245 have been identified as Arl1 effectors. Golgins are large coiled-coil proteins found in the Golgi and these golgins contain a C-terminal GRIP domain which is the site of Arl1 binding. Additional Arl1 effectors include the GARP (Golgi-associated retrograde protein)/VFT (Vps53) vesicle-tethering complex and Arfaptin 2. Arl1 is not required for exocytosis but appears necessary for trafficking from the endosomes to the Golgi. In Drosophila zygotes mutation of Arl1 is lethal and in the host-bloodstream form of Trypanosoma brucei Arl1 is essential for viability.
rpsblast_kog	gnl\|CDD\|35300	2	173	172	Gaps:3	90.67	193	68.00	10.29	2e-76	KOG0077 KOG0077 KOG0077 Vesicle coat complex COPII GTPase subunit SAR1 [Intracellular trafficking secretion and vesicular transport].
	gnl\|CDD\|35293	2	171	170	Gaps:9	88.95	181	36.02	19.88	3e-31	KOG0070 KOG0070 KOG0070 GTP-binding ADP-ribosylation factor Arf1 [Intracellular trafficking secretion and vesicular transport].
	gnl\|CDD\|35294	2	171	170	Gaps:9	89.44	180	35.40	19.88	1e-26	KOG0071 KOG0071 KOG0071 GTP-binding ADP-ribosylation factor Arf6 (dArf3) [Intracellular trafficking secretion and vesicular transport].
	gnl\|CDD\|35296	3	169	167	Gaps:8	85.95	185	33.33	17.61	1e-23	KOG0073 KOG0073 KOG0073 GTP-binding ADP-ribosylation factor-like protein ARL2 [Intracellular trafficking secretion and vesicular transport Cytoskeleton].
	gnl\|CDD\|35295	2	171	170	Gaps:9	88.46	182	31.06	24.22	4e-22	KOG0072 KOG0072 KOG0072 GTP-binding ADP-ribosylation factor-like protein ARL1 [Intracellular trafficking secretion and vesicular transport].

Other analysis

Functional Gbrowse

Structural Gbrowse

Genome Mapping at gene locus

Analysis	Hit name	RefStart	RefEnd	Hit length	Hit identity	Hit coverage	eValue	Hit description
blastx_uniprot_trembl	A7EPS1	333246	333817	189	87.01	81.48	n/a	GTP-binding protein SARA - Sclerotinia sclerotiorum (strain ATCC 18683 / 1980 / Ss-1) (White mold) (Whetzelinia sclerotiorum)
	A6SR17	333246	333817	189	87.01	81.48	n/a	GTP-binding protein sarA - Botryotinia fuckeliana (strain B05.10) (Noble rot fungus) (Botrytis cinerea)
	A8PWA2	333246	333817	231	72.08	66.67	n/a	Putative uncharacterized protein - Malassezia globosa CBS 7966
	A6ZW37	333246	333817	190	74.34	80.00	n/a	ARF family protein - Saccharomyces cerevisiae (strain YJM789) (Baker's yeast)
	A7TE70	333246	333817	188	73.68	80.85	n/a	Putative uncharacterized protein - Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294) (Kluyveromyces polysporus)
	Q567Y5	333342	333817	198	66.43	72.22	n/a	SAR1 gene homolog A - Danio rerio (Zebrafish) (Brachydanio rerio)
	Q6PBX2	333342	333817	198	65.03	72.22	n/a	SAR1a gene homolog 2 - Danio rerio (Zebrafish) (Brachydanio rerio)
	Q6P4X3	333342	333817	198	64.34	72.22	n/a	SAR1a protein - Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
	Q66J32	333342	333817	198	64.34	72.22	n/a	MGC82076 protein - Xenopus laevis (African clawed frog)
	Q5CZL6	333342	333817	198	62.24	72.22	n/a	MGC108053 protein - Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
blastx_FNG31_prot	SNU07794.1	333246	333817	167	94.81	92.22	n/a	Stagonospora nodorum hypothetical protein translation
	SS1G_07320.1	333246	333817	189	87.01	81.48	n/a	sclerotinia_sclerotiorum GTP-binding protein SARA translation 190 aa
	BC1G_15213.1	333246	333817	189	87.01	81.48	n/a	Botrytis cinerea hypothetical protein similar to SARA_ASPNG GTP-binding protein SARA translation 190 aa
	AO090003000842	333246	333817	189	87.66	81.48	n/a	Aspergillus oryzae vesicle coat complex COPII GTPase subunit SAR1
	gi:70990788:ref:XP_750243.1:	333246	333817	189	87.01	81.48	n/a	Aspergillus fumigatus Af293 GTP binding protein SarA
	MGG_06362.5	333246	333817	189	84.42	81.48	n/a	magnaporthe_grisea GTP-binding protein SAR1 translation 190 aa
	AN0411	333246	333817	189	85.71	81.48	n/a	Aspergillus nidulans AN0411.3 GTP-binding protein sarA translation 190 aa
	CHG02899.1	333246	333814	188	83.66	81.38	n/a	Chaetomium globosum hypothetical protein translation 189 aa
	FG06646.1	333246	333817	189	83.12	81.48	n/a	fusarium_graminearum SAR1_TRIRE GTP-binding protein SAR1 190 aa
	NCU00381.2	333246	333868	1154	80.36	14.56	n/a	Neurospora crassa hypothetical protein translation 1155 aa
blat_BES	no results
blastx_SN15proteins	gnl:WGS:AAGI:SNOT_07794.2:gb:EAT85260	333246	333817	173	94.81	89.02	n/a	n/a
RepeatMasker_SR	no results
RepeatMasker_LC	no results
sim4_Lm_allest	RS0AAE12YM06CM1	332757	333530	445	98.88	100.00	n/a	Lm isolate JN3 - Conidia germinating in liquid Fries for 48 h (insert up to 1kb)
sim4_lm_allest6_clctg	RS0AAE12YM06CM1	332757	333530	445	98.88	100.00	n/a	Lm isolate JN3 - Conidia germinating in liquid Fries for 48 h (insert up to 1kb)
sim4_cDNA_BHowlett	no results
blastx_uniprot_sprot	Q0UKC0	333246	333817	185	94.81	83.24	n/a	Small COPII coat GTPase SAR1 - Phaeosphaeria nodorum (Septoria nodorum)
	P0C583	333246	333817	189	85.71	81.48	n/a	Small COPII coat GTPase sar-1 - Neurospora crassa
	Q877B9	333246	333817	189	87.66	81.48	n/a	Small COPII coat GTPase sar1 - Aspergillus oryzae
	P52886	333246	333817	189	87.01	81.48	n/a	Small COPII coat GTPase SAR1 - Aspergillus niger
	A1CRG9	333246	333817	189	87.01	81.48	n/a	Small COPII coat GTPase sar1 - Aspergillus clavatus
	A1D4D1	333246	333817	189	87.01	81.48	n/a	Small COPII coat GTPase sar1 - Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / NRRL 181) (Aspergillus fischerianus (strain ATCC 1020 / DSM 3700 / NRRL 181))
	Q4WJS7	333246	333817	189	87.01	81.48	n/a	Small COPII coat GTPase sar1 - Aspergillus fumigatus (Sartorya fumigata)
	Q0CUN7	333246	333817	189	86.36	81.48	n/a	Small COPII coat GTPase sar1 - Aspergillus terreus (strain NIH 2624)
	Q5EMZ6	333246	333817	189	84.42	81.48	n/a	Small COPII coat GTPase SAR1 - Magnaporthe grisea (Rice blast fungus) (Pyricularia grisea)
	Q5BGB9	333246	333817	189	85.71	81.48	n/a	Small COPII coat GTPase sar1 - Emericella nidulans (Aspergillus nidulans)
REPET	no results
MUMer_show_coords_lm_WA74	no results