GRS - Genome Report System

Leptosphaeria maculans GRS

Functional annotation

Lema_P034380.1

Gene Identifier

DbXrefs

Domain/Motif (Interproscan results)

Localization / Targeting

Predicted function

Blast based analysis

Analysis	Hit	start	end	length	Note	Hit coverage	Hit length	Hit pident	Hit pcons	eValue	Hit description
blastp_kegg	ncr:NCU03407	45	250	206	n/a	76.87	268	77.67	7.28	1e-108	protein rho1
	afm:AFUA_5G14060	16	280	265	n/a	90.91	286	66.92	10.38	1e-106	Rho GTPase Rho4
	ani:AN2687.2	16	280	265	n/a	90.36	280	67.98	11.07	1e-105	similar to AF379682_1 Rho3 GTPase
	aor:AO090113000121	57	275	219	n/a	96.48	227	71.23	9.59	1e-103	Ras-related small GTPase Rho type
	pan:PODANSg6937	43	249	207	n/a	69.46	298	74.40	6.28	1e-102	hypothetical protein
	nfi:NFIA_072930	27	253	227	n/a	83.91	261	73.97	8.22	1e-102	Rho GTPase Rho4 putative
	fgr:FG00304.1	38	249	212	n/a	78.52	270	71.70	11.32	1e-101	hypothetical protein
	ang:An14g05530	57	280	224	n/a	88.16	228	77.61	7.96	1e-101	hypothetical protein
	mgr:MGG_03901	57	261	205	n/a	70.21	292	73.17	9.76	1e-101	hypothetical protein
	ssl:SS1G_02182	59	262	204	n/a	91.89	222	73.04	9.80	1e-100	similar to Rho3 GTPase
blastp_uniprot_sprot	sp\|Q96WY0\|RHOC_EMENI	16	280	265	n/a	90.36	280	67.98	11.07	1e-106	GTP-binding protein rhoC OS Emericella nidulans GN rhoC PE 3 SV 2
	sp\|Q874R1\|RHO4_SCHPO	66	251	186	n/a	91.63	203	63.44	16.13	4e-81	GTP-binding protein rho4 OS Schizosaccharomyces pombe GN rho4 PE 1 SV 2
	sp\|P61589\|RHOA_RAT	67	241	175	n/a	86.01	193	63.25	15.66	2e-61	Transforming protein RhoA OS Rattus norvegicus GN Rhoa PE 2 SV 1
	sp\|Q5REY6\|RHOA_PONAB	67	241	175	n/a	86.01	193	63.25	15.66	2e-61	Transforming protein RhoA OS Pongo abelii GN RHOA PE 2 SV 2
	sp\|Q9QUI0\|RHOA_MOUSE	67	241	175	n/a	86.01	193	63.25	15.66	2e-61	Transforming protein RhoA OS Mus musculus GN Rhoa PE 1 SV 1
	sp\|P61586\|RHOA_HUMAN	67	241	175	n/a	86.01	193	63.25	15.66	2e-61	Transforming protein RhoA OS Homo sapiens GN RHOA PE 1 SV 1
	sp\|P61585\|RHOA_BOVIN	67	241	175	n/a	86.01	193	63.25	15.66	2e-61	Transforming protein RhoA OS Bos taurus GN RHOA PE 1 SV 1
	sp\|P24406\|RHOA_CANFA	67	241	175	n/a	86.01	193	63.25	15.06	5e-61	Transforming protein RhoA OS Canis familiaris GN RHOA PE 2 SV 1
	sp\|P48148\|RHO1_DROME	64	241	178	n/a	88.02	192	60.95	15.98	1e-60	Ras-like GTP-binding protein Rho1 OS Drosophila melanogaster GN Rho1 PE 1 SV 1
	sp\|Q22038\|RHO1_CAEEL	67	246	180	n/a	89.06	192	59.65	17.54	1e-60	Ras-like GTP-binding protein rhoA OS Caenorhabditis elegans GN rho-1 PE 2 SV 1
blastp_pdb	2rgn_F	67	241	175	n/a	84.69	196	63.25	15.66	4e-62	mol:protein length:196 Transforming protein RhoA
	2rgn_C	67	241	175	n/a	84.69	196	63.25	15.66	4e-62	mol:protein length:196 Transforming protein RhoA
	1x86_H	67	241	175	n/a	84.69	196	63.25	15.66	4e-62	mol:protein length:196 Transforming protein RhoA
	1x86_F	67	241	175	n/a	84.69	196	63.25	15.66	4e-62	mol:protein length:196 Transforming protein RhoA
	1x86_D	67	241	175	n/a	84.69	196	63.25	15.66	4e-62	mol:protein length:196 Transforming protein RhoA
	1x86_B	67	241	175	n/a	84.69	196	63.25	15.66	4e-62	mol:protein length:196 Transforming protein RhoA
	1lb1_H	67	241	175	n/a	86.46	192	63.25	15.66	4e-62	mol:protein length:192 Transforming protein RhoA
	1lb1_F	67	241	175	n/a	86.46	192	63.25	15.66	4e-62	mol:protein length:192 Transforming protein RhoA
	1lb1_D	67	241	175	n/a	86.46	192	63.25	15.66	4e-62	mol:protein length:192 Transforming protein RhoA
	1lb1_B	67	241	175	n/a	86.46	192	63.25	15.66	4e-62	mol:protein length:192 Transforming protein RhoA
rpsblast_cdd	gnl\|CDD\|133332	67	252	186	Gaps:10	95.19	187	64.04	14.04	1e-82	cd04132 Rho4_like Rho4-like subfamily. Rho4 is a GTPase that controls septum degradation by regulating secretion of Eng1 or Agn1 during cytokinesis. Rho4 also plays a role in cell morphogenesis. Rho4 regulates septation and cell morphology by controlling the actin cytoskeleton and cytoplasmic microtubules. The localization of Rho4 is modulated by Rdi1 which may function as a GDI and by Rga9 which is believed to function as a GAP. In S. pombe both Rho4 deletion and Rho4 overexpression result in a defective cell wall suggesting a role for Rho4 in maintaining cell wall integrity. Most Rho proteins contain a lipid modification site at the C-terminus with a typical sequence motif CaaX where a an aliphatic amino acid and X any amino acid. Lipid binding is essential for membrane attachment a key feature of most Rho proteins.
	gnl\|CDD\|128471	69	237	169	Gaps:3	96.55	174	60.12	14.88	2e-77	smart00174 RHO Rho (Ras homology) subfamily of Ras-like small GTPases. Members of this subfamily of Ras-like small GTPases include Cdc42 and Rac as well as Rho isoforms.
	gnl\|CDD\|133251	68	237	170	Gaps:4	98.25	171	57.14	20.24	2e-72	cd00157 Rho Rho (Ras homology) family. Members of the Rho family include RhoA Cdc42 Rac Rnd Wrch1 RhoBTB and Rop. There are 22 human Rho family members identified currently. These proteins are all involved in the reorganization of the actin cytoskeleton in response to external stimuli. They also have roles in cell transformation by Ras in cytokinesis in focal adhesion formation and in the stimulation of stress-activated kinase. These various functions are controlled through distinct effector proteins and mediated through a GTP-binding/GTPase cycle involving three classes of regulating proteins: GAPs (GTPase-activating proteins) GEFs (guanine nucleotide exchange factors) and GDIs (guanine nucleotide dissociation inhibitors). Most Rho proteins contain a lipid modification site at the C-terminus with a typical sequence motif CaaX where a an aliphatic amino acid and X any amino acid. Lipid binding is essential for membrane attachment a key feature of most Rho proteins. Since crystal structures often lack C-terminal residues this feature is not available for annotation in many of the CDs in the hierarchy.
	gnl\|CDD\|133273	67	240	174	Gaps:3	98.86	175	60.69	14.45	8e-69	cd01870 RhoA_like RhoA-like subfamily. The RhoA subfamily consists of RhoA RhoB and RhoC. RhoA promotes the formation of stress fibers and focal adhesions regulating cell shape attachment and motility. RhoA can bind to multiple effector proteins thereby triggering different downstream responses. In many cell types RhoA mediates local assembly of the contractile ring which is necessary for cytokinesis. RhoA is vital for muscle contraction in vascular smooth muscle cells RhoA plays a key role in cell contraction differentiation migration and proliferation. RhoA activities appear to be elaborately regulated in a time- and space-dependent manner to control cytoskeletal changes. Most Rho proteins contain a lipid modification site at the C-terminus with a typical sequence motif CaaX where a an aliphatic amino acid and X any amino acid. Lipid binding is essential for membrane attachment a key feature of most Rho proteins. RhoA and RhoC are observed only in geranylgeranylated forms however RhoB can be present in palmitoylated farnesylated and geranylgeranylated forms. RhoA and RhoC are highly relevant for tumor progression and invasiveness however RhoB has recently been suggested to be a tumor suppressor. Due to the presence of truncated sequences in this CD the lipid modification site is not available for annotation.
	gnl\|CDD\|133274	68	237	170	Gaps:3	97.13	174	57.40	17.16	5e-58	cd01871 Rac1_like Rac1-like subfamily. The Rac1-like subfamily consists of Rac1 Rac2 and Rac3 proteins plus the splice variant Rac1b that contains a 19-residue insertion near switch II relative to Rac1. While Rac1 is ubiquitously expressed Rac2 and Rac3 are largely restricted to hematopoietic and neural tissues respectively. Rac1 stimulates the formation of actin lamellipodia and membrane ruffles. It also plays a role in cell-matrix adhesion and cell anoikis. In intestinal epithelial cells Rac1 is an important regulator of migration and mediates apoptosis. Rac1 is also essential for RhoA-regulated actin stress fiber and focal adhesion complex formation. In leukocytes Rac1 and Rac2 have distinct roles in regulating cell morphology migration and invasion but are not essential for macrophage migration or chemotaxis. Rac3 has biochemical properties that are closely related to Rac1 such as effector interaction nucleotide binding and hydrolysis Rac2 has a slower nucleotide association and is more efficiently activated by the RacGEF Tiam1. Both Rac1 and Rac3 have been implicated in the regulation of cell migration and invasion in human metastatic breast cancer. Most Rho proteins contain a lipid modification site at the C-terminus with a typical sequence motif CaaX where a an aliphatic amino acid and X any amino acid. Lipid binding is essential for membrane attachment a key feature of most Rho proteins. Due to the presence of truncated sequences in this CD the lipid modification site is not available for annotation.
rpsblast_kog	gnl\|CDD\|35614	66	237	172	Gaps:2	86.87	198	56.98	17.44	3e-67	KOG0393 KOG0393 KOG0393 Ras-related small GTPase Rho type [General function prediction only].
	gnl\|CDD\|35301	60	233	174	Gaps:15	77.78	207	31.68	19.88	7e-24	KOG0078 KOG0078 KOG0078 GTP-binding protein SEC4 small G protein superfamily and related Ras family GTP-binding proteins [Signal transduction mechanisms Intracellular trafficking secretion and vesicular transport].
	gnl\|CDD\|35315	68	278	211	Gaps:21	97.00	200	26.80	19.07	6e-21	KOG0092 KOG0092 KOG0092 GTPase Rab5/YPT51 and related small G protein superfamily GTPases [Intracellular trafficking secretion and vesicular transport].
	gnl\|CDD\|35307	65	233	169	Gaps:16	76.59	205	31.85	21.02	3e-20	KOG0084 KOG0084 KOG0084 GTPase Rab1/YPT1 small G protein superfamily and related GTP-binding proteins [Signal transduction mechanisms Intracellular trafficking secretion and vesicular transport].
	gnl\|CDD\|35317	62	255	194	Gaps:16	82.35	221	28.57	19.78	6e-20	KOG0094 KOG0094 KOG0094 GTPase Rab6/YPT6/Ryh1 small G protein superfamily [Intracellular trafficking secretion and vesicular transport].

Other analysis

Functional Gbrowse

Structural Gbrowse

Genome Mapping at gene locus

Analysis	Hit name	RefStart	RefEnd	Hit length	Hit identity	Hit coverage	eValue	Hit description
blastx_uniprot_trembl	Q0UHY4	856721	857687	277	90.91	99.28	n/a	Putative uncharacterized protein - Phaeosphaeria nodorum (Septoria nodorum)
	Q0CJL9	856754	857687	283	65.43	95.05	n/a	Protein rho4 - Aspergillus terreus (strain NIH 2624)
	A1CBZ0	856709	857687	286	62.59	100.00	n/a	Rho GTPase Rho4 putative - Aspergillus clavatus
	Q5B9U3	856754	857687	280	65.79	95.00	n/a	Putative uncharacterized protein - Emericella nidulans (Aspergillus nidulans)
	Q4WW13	856754	857687	286	63.24	95.10	n/a	Rho GTPase Rho4 putative - Aspergillus fumigatus (Sartorya fumigata)
	A1DDC1	856787	857687	261	66.28	100.00	n/a	Rho GTPase Rho4 putative - Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / NRRL 181) (Aspergillus fischerianus (strain ATCC 1020 / DSM 3700 / NRRL 181))
	Q7RY07	856841	857597	268	77.67	76.87	n/a	Protein rho1 - Neurospora crassa
	Q1EB09	856877	857687	225	76.17	95.11	n/a	Putative uncharacterized protein - Coccidioides immitis
	Q8X0M2	856883	857597	251	80.73	76.49	n/a	Probable RHO1 PROTEIN - Neurospora crassa
	Q2GV96	856883	857630	257	77.56	79.77	n/a	Putative uncharacterized protein - Chaetomium globosum (Soil fungus)
	A1CBZ0	856709	857687	286	62.59	100.00	n/a	Rho GTPase Rho4 putative - Aspergillus clavatus
blastx_FNG31_prot	SNU08630.1	856721	857687	277	90.91	99.28	n/a	Stagonospora nodorum hypothetical protein translation
	AN2687	856754	857687	280	65.79	95.00	n/a	Aspergillus nidulans AN2687.3 hypothetical protein translation 281 aa
	gi:70996993:ref:XP_753251.1:	856754	857687	286	63.24	95.10	n/a	Aspergillus fumigatus Af293 Rho GTPase Rho4
	jgi:Triree:32512	856799	857687	269	67.06	93.68	n/a	Trichoderma reesei
	CIMG_00254.2	856877	857687	225	76.17	95.11	n/a	Coccidioides immitis conserved hypothetical protein translation 226 aa
	CHG08108.1	856883	857630	257	77.56	79.77	n/a	Chaetomium globosum conserved hypothetical protein translation 258 aa
	AO090113000121	856877	857687	227	71.30	98.24	n/a	Aspergillus oryzae Ras-related small GTPase Rho type
	MGG_03901.5	856826	857687	292	65.08	86.30	n/a	magnaporthe_grisea conserved hypothetical protein translation 293 aa
	FG00304.1	856811	857687	270	65.34	92.96	n/a	fusarium_graminearum hypothetical protein 271 aa
	NCU03407.2	856679	857597	337	64.42	79.23	n/a	Neurospora crassa hypothetical protein translation 338 aa
blat_BES	QS0AAD10ZB04RM1	832683	891114	916	n/a	n/a	n/a	n/a
	QS0AAE114ZG12RM1	832236	887371	1095	n/a	n/a	n/a	n/a
	QS0AAE112ZE01FM1	832608	883123	1038	n/a	n/a	n/a	n/a
blastx_SN15proteins	gnl:WGS:AAGI:SNOT_08630.2:gb:EAT83798	856721	857687	277	90.91	99.28	n/a	n/a
	gnl:WGS:AAGI:SNOT_04837.2:gb:EAT87228	856907	857483	171	62.50	84.21	n/a	n/a
	gnl:WGS:AAGI:SNOT_00327.2:gb:EAT91822	856892	857558	200	51.43	87.50	n/a	n/a
	gnl:WGS:AAGI:SNOT_12991.2:gb:EAT79791	856910	857570	199	49.71	86.93	n/a	n/a
	gnl:WGS:AAGI:SNOT_08068.2:gb:EAT84344	856895	857570	201	48.02	88.06	n/a	n/a
	gnl:WGS:AAGI:SNOT_00974.2:gb:EAT92469	856907	857570	200	47.70	87.00	n/a	n/a
RepeatMasker_SR	no results
RepeatMasker_LC	no results
sim4_Lm_allest	RS0AAC15YA20FM1	856795	857523	589	99.32	100.00	n/a	Lm isolate M1 - V8 (5 days) and minimal medium (8 hours) mycelial library
sim4_lm_allest6_clctg	RS0AAC15YA20FM1	856795	857523	589	99.32	100.00	n/a	Lm isolate M1 - V8 (5 days) and minimal medium (8 hours) mycelial library
sim4_cDNA_BHowlett	no results
blastx_uniprot_sprot	Q96WY0	856877	857687	230	72.85	96.09	n/a	GTP-binding protein rhoC precursor - Emericella nidulans (Aspergillus nidulans)
	Q874R1	856904	857600	203	63.44	91.63	n/a	GTP-binding protein rho4 precursor - Schizosaccharomyces pombe (Fission yeast)
	P61589	856907	857570	193	60.92	90.16	n/a	Transforming protein RhoA precursor - Rattus norvegicus (Rat)
	Q5REY6	856907	857570	193	60.92	90.16	n/a	Transforming protein RhoA precursor - Pongo pygmaeus (Orangutan)
	Q9QUI0	856907	857570	193	60.92	90.16	n/a	Transforming protein RhoA precursor - Mus musculus (Mouse)
	P61586	856907	857570	193	60.92	90.16	n/a	Transforming protein RhoA precursor - Homo sapiens (Human)
	P61585	856907	857570	193	60.92	90.16	n/a	Transforming protein RhoA precursor - Bos taurus (Bovine)
	P24406	856907	857570	193	60.92	90.16	n/a	Transforming protein RhoA precursor - Canis familiaris (Dog)
	P48148	856898	857603	192	56.99	96.88	n/a	Ras-like GTP-binding protein Rho1 precursor - Drosophila melanogaster (Fruit fly)
	Q22038	856907	857585	192	57.54	93.23	n/a	Ras-like GTP-binding protein rhoA precursor - Caenorhabditis elegans
REPET	no results
MUMer_show_coords_lm_WA74	no results