GRS - Genome Report System

Leptosphaeria maculans GRS

Functional annotation

Lema_P004950.1

Gene Identifier

DbXrefs

Domain/Motif (Interproscan results)

Localization / Targeting

Predicted function

Blast based analysis

Analysis	Hit	start	end	length	Note	Hit coverage	Hit length	Hit pident	Hit pcons	eValue	Hit description
blastp_kegg	ani:AN4643.2	26	418	393	n/a	70.42	551	43.56	20.10	2e-89	hypothetical protein
	pan:PODANSg8531	19	421	403	n/a	76.00	475	40.17	21.33	7e-77	hypothetical protein
	ani:AN7359.2	24	409	386	n/a	57.59	514	42.91	18.92	3e-68	hypothetical protein
	aor:AO090103000132	42	421	380	n/a	57.51	506	41.58	19.59	5e-66	cytochrome P450 CYP3/CYP5/CYP6/CYP9 subfamilies
	nfi:NFIA_005630	33	409	377	n/a	58.48	513	42.00	17.00	7e-66	cytochrome P450 pisatin demethylase putative
	afm:AFUA_3G03450	33	409	377	n/a	58.14	516	41.67	17.33	1e-65	cytochrome P450 oxidoreductase (EC:1.14.-.-) K00517
	ang:An08g07910	15	409	395	n/a	62.53	483	43.38	18.21	5e-60	hypothetical protein
	fgr:FG08008.1	34	409	376	n/a	21.31	1300	42.96	17.69	2e-59	hypothetical protein
	ssl:SS1G_01757	33	409	377	n/a	49.22	512	45.24	17.06	3e-56	hypothetical protein
	aor:AO090005000002	24	412	389	n/a	72.56	532	42.23	20.98	3e-56	cytochrome P450 CYP3/CYP5/CYP6/CYP9 subfamilies
blastp_uniprot_sprot	sp\|P38364\|PID6_FUSSO	17	409	393	n/a	57.71	506	37.33	17.81	4e-47	Pisatin demethylase OS Fusarium solani subsp. pisi GN PDA6-1 PE 3 SV 1
	sp\|Q12645\|PID9_FUSSO	17	409	393	n/a	58.45	515	36.21	17.61	4e-46	Pisatin demethylase OS Fusarium solani subsp. pisi GN PDAT9 PE 3 SV 1
	sp\|Q12609\|STCF_EMENI	20	409	390	n/a	33.68	478	38.51	21.12	7e-24	Probable sterigmatocystin biosynthesis P450 monooxygenase stcF OS Emericella nidulans GN stcF PE 3 SV 2
	sp\|P17549\|CP53_ASPNG	42	396	355	n/a	45.45	517	33.62	17.02	5e-22	Benzoate 4-monooxygenase OS Aspergillus niger GN bphA PE 1 SV 1
	sp\|Q12732\|AVNA_ASPPA	34	409	376	n/a	36.77	495	36.81	20.88	5e-21	Averantin oxidoreductase OS Aspergillus parasiticus GN avnA PE 1 SV 2
	sp\|Q00707\|STCL_EMENI	26	412	387	n/a	41.73	496	32.37	18.36	8e-21	Probable sterigmatocystin biosynthesis P450 monooxygenase stcL OS Emericella nidulans GN stcL PE 3 SV 2
	sp\|Q12608\|STCB_EMENI	41	407	367	n/a	34.04	517	32.95	18.75	6e-16	Probable sterigmatocystin biosynthesis P450 monooxygenase STCB OS Emericella nidulans GN stcB PE 3 SV 2
	sp\|O13317\|TRI11_FUSSP	26	386	361	n/a	41.87	492	29.13	22.33	5e-15	Isotrichodermin C-15 hydroxylase OS Fusarium sporotrichioides GN TRI11 PE 3 SV 1
	sp\|O00061\|CP67_UROFA	34	412	379	n/a	31.24	541	30.77	22.49	3e-14	Cytochrome P450 67 (Fragment) OS Uromyces fabae GN CYP67 PE 2 SV 1
	sp\|Q12612\|TRI4_FUSSP	20	349	330	n/a	38.27	520	30.15	22.11	1e-13	Trichodiene oxygenase OS Fusarium sporotrichioides GN TRI4 PE 3 SV 1
blastp_pdb	no results
rpsblast_cdd	gnl\|CDD\|109135	67	413	347	Gaps:30	74.29	459	24.63	15.84	6e-36	pfam00067 p450 Cytochrome P450. Cytochrome P450s are haem-thiolate proteins involved in the oxidative degradation of various compounds. They are particularly well known for their role in the degradation of environmental toxins and mutagens. They can be divided into 4 classes according to the method by which electrons from NAD(P)H are delivered to the catalytic site. Sequence conservation is relatively low within the family - there are only 3 absolutely conserved residues - but their general topography and structural fold are highly conserved. The conserved core is composed of a coil termed the 'meander' a four-helix bundle helices J and K and two sets of beta-sheets. These constitute the haem-binding loop (with an absolutely conserved cysteine that serves as the 5th ligand for the haem iron) the proton-transfer groove and the absolutely conserved EXXR motif in helix K. While prokaryotic P450s are soluble proteins most eukaryotic P450s are associated with microsomal membranes. their general enzymatic function is to catalyse regiospecific and stereospecific oxidation of non-activated hydrocarbons at physiological temperatures.
	gnl\|CDD\|32307	58	421	364	Gaps:69	76.64	411	23.49	14.92	4e-11	COG2124 CypX Cytochrome P450 [Secondary metabolites biosynthesis transport and catabolism].
	gnl\|CDD\|131542	264	329	66	Gaps:14	11.13	719	28.75	17.50	1.2	TIGR02489 flgE_epsilon Members of this family are flagellar hook proteins designated FlgE as found in the epsilon subdivision of the Proteobacteria (Helicobacter Wolinella and Campylobacter). These proteins differ significantly in architecture from proteins designated FlgE in other lineages the N-terminal and C-terminal domains are homologous but members of this family only contain a large central domain that is surface-exposed and variable between strains.
	gnl\|CDD\|116206	75	175	101	Gaps:8	37.60	258	32.99	8.25	1.4	pfam07587 PSD1 Protein of unknown function (DUF1553). A family of proteins found in Rhodopirellula baltica.
	gnl\|CDD\|138321	254	315	62	Gaps:17	13.65	491	32.84	19.40	1.5	PRK10982 PRK10982 galactose/methyl galaxtoside transporter ATP-binding protein Provisional.
rpsblast_kog	gnl\|CDD\|35379	17	409	393	Gaps:23	76.86	497	20.16	17.54	2e-25	KOG0157 KOG0157 KOG0157 Cytochrome P450 CYP4/CYP19/CYP26 subfamilies [Secondary metabolites biosynthesis transport and catabolism Lipid transport and metabolism].
	gnl\|CDD\|35381	36	409	374	Gaps:50	71.68	519	24.73	13.44	2e-25	KOG0159 KOG0159 KOG0159 Cytochrome P450 CYP11/CYP12/CYP24/CYP27 subfamilies [Secondary metabolites biosynthesis transport and catabolism].
	gnl\|CDD\|35378	20	413	394	Gaps:38	78.94	489	24.09	16.32	7e-24	KOG0156 KOG0156 KOG0156 Cytochrome P450 CYP2 subfamily [Secondary metabolites biosynthesis transport and catabolism].
	gnl\|CDD\|35380	16	384	369	Gaps:44	73.15	499	22.19	15.07	6e-20	KOG0158 KOG0158 KOG0158 Cytochrome P450 CYP3/CYP5/CYP6/CYP9 subfamilies [Secondary metabolites biosynthesis transport and catabolism].
	gnl\|CDD\|35903	265	412	148	Gaps:13	28.60	486	26.62	15.11	5e-09	KOG0684 KOG0684 KOG0684 Cytochrome P450 [Secondary metabolites biosynthesis transport and catabolism].

Other analysis

Functional Gbrowse

Structural Gbrowse

Genome Mapping at gene locus

Analysis	Hit name	RefStart	RefEnd	Hit length	Hit identity	Hit coverage	eValue	Hit description
blastx_uniprot_trembl	A2QS12	1900606	1901846	483	39.21	78.67	n/a	Function: PDAT9 of N. haematococca detoxifies the phytoalexin pisatin - Aspergillus niger
	Q0UBH8	1900426	1901714	455	68.78	90.11	n/a	Putative uncharacterized protein - Phaeosphaeria nodorum (Septoria nodorum)
	Q0CEV8	1900426	1901807	502	45.98	86.65	n/a	Putative uncharacterized protein - Aspergillus terreus (strain NIH 2624)
	Q2UTI6	1900426	1901819	532	40.43	88.35	n/a	Cytochrome P450 CYP3/CYP5/CYP6/CYP9 subfamilies - Aspergillus oryzae
	A6QWT4	1900426	1901789	508	39.02	80.71	n/a	Predicted protein - Ajellomyces capsulata (strain NAm1) (Histoplasma capsulatum)
	Q1EBB0	1900414	1901831	484	36.34	86.98	n/a	Putative uncharacterized protein - Coccidioides immitis
	Q0CBG7	1900426	1901705	451	38.89	87.80	n/a	Predicted protein - Aspergillus terreus (strain NIH 2624)
	Q2GMQ8	1900426	1901792	517	36.05	83.17	n/a	Putative uncharacterized protein - Chaetomium globosum (Soil fungus)
	Q0V1D0	1900426	1901792	512	33.73	81.64	n/a	Putative uncharacterized protein - Phaeosphaeria nodorum (Septoria nodorum)
blastx_FNG31_prot	SNU16391.1	1900944	1901888	315	45.40	100.00	n/a	Stagonospora nodorum hypothetical protein translation
	FG03008.1	1900429	1901831	596	33.71	74.16	n/a	fusarium_graminearum hypothetical protein 597 aa
	AO090005000002	1900426	1901819	532	40.43	88.35	n/a	Aspergillus oryzae cytochrome P450 CYP3/CYP5/CYP6/CYP9 subfamilies
	CIMG_00153.2	1900414	1901831	484	36.34	86.98	n/a	Coccidioides immitis hypothetical protein translation 485 aa
	CHG10746.1	1900426	1901792	517	36.05	83.17	n/a	Chaetomium globosum hypothetical protein translation 518 aa
	SNU02184.1	1900426	1901792	512	33.73	81.64	n/a	Stagonospora nodorum hypothetical protein translation
	FG03008.1	1900429	1901831	596	33.71	74.16	n/a	fusarium_graminearum hypothetical protein 597 aa
	BC1G_06047.1	1900426	1901825	564	31.29	83.87	n/a	Botrytis cinerea hypothetical protein translation 565 aa
	AN4643	1900426	1901831	551	41.31	88.75	n/a	Aspergillus nidulans AN4643.3 conserved hypothetical protein translation 552 aa
	AO090023000045	1900426	1901846	504	31.28	86.90	n/a	Aspergillus oryzae cytochrome P450 CYP2 subfamily
	SNU10886.1	1900510	1901900	486	67.50	90.53	n/a	Stagonospora nodorum hypothetical protein translation
blat_BES	QS0AAE121ZB06RM1	1901108	1901565	821	n/a	n/a	n/a	n/a
blastx_SN15proteins	gnl:WGS:AAGI:SNOT_08994.2:gb:EAT83186	1900474	1901351	318	40.37	84.91	n/a	n/a
	gnl:WGS:AAGI:SNOT_20148.2:gb:	1900905	1901831	538	31.53	58.36	n/a	n/a
	gnl:WGS:AAGI:SNOT_10886.2:gb:EAT81385	1900426	1901714	455	68.78	90.11	n/a	n/a
	gnl:WGS:AAGI:SNOT_02184.2:gb:EAT90396	1900426	1901792	512	33.73	81.64	n/a	n/a
	gnl:WGS:AAGI:SNOT_12323.2:gb:EAT80136	1900414	1901831	489	39.95	75.26	n/a	n/a
	gnl:WGS:AAGI:SNOT_11796.2:gb:EAT80840	1900426	1900887	356	46.20	44.38	n/a	n/a
	gnl:WGS:AAGI:SNOT_04196.2:gb:EAT87956	1900426	1900884	382	48.32	39.01	n/a	n/a
	gnl:WGS:AAGI:SNOT_02648.2:gb:EAT89379	1900414	1900884	341	40.62	46.92	n/a	n/a
	gnl:WGS:AAGI:SNOT_15356.2:gb:EAT77289	1900414	1900884	499	42.68	31.46	n/a	n/a
RepeatMasker_SR	no results
RepeatMasker_LC	no results
sim4_Lm_allest	RS0AAG8YM14CM1	1900429	1900982	710	99.20	70.28	n/a	Lm isolate JN3 - Ungerminated conidia (V8 agar) (insert up to 1kb)
sim4_Lm_allest	RS0AAG8YM14CM1	1900429	1900982	710	99.20	70.28	n/a	Lm isolate JN3 - Ungerminated conidia (V8 agar) (insert up to 1kb)
sim4_lm_allest6_clctg	RS0AAG8YM14CM1	1900429	1900982	710	99.20	70.28	n/a	Lm isolate JN3 - Ungerminated conidia (V8 agar) (insert up to 1kb)
sim4_lm_allest6_clctg	RS0AAG8YM14CM1	1900429	1900982	710	99.20	70.28	n/a	Lm isolate JN3 - Ungerminated conidia (V8 agar) (insert up to 1kb)
sim4_cDNA_BHowlett	no results
blastx_uniprot_sprot	P17549	1900429	1901822	517	28.86	86.46	n/a	Benzoate 4-monooxygenase - Aspergillus niger
	P17549	1900429	1901822	517	28.86	86.46	n/a	Benzoate 4-monooxygenase - Aspergillus niger
	P38364	1900426	1901840	506	38.06	71.15	n/a	Pisatin demethylase - Fusarium solani subsp. pisi (Nectria haematococca)
	Q12645	1900426	1901840	515	38.33	69.90	n/a	Pisatin demethylase - Fusarium solani subsp. pisi (Nectria haematococca)
REPET	no results
MUMer_show_coords_lm_WA74	no results