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Spads

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  • Version: 1.2.0
  • Access: free acces url 

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  • Spads

Spads


Specific Primers & Amplicon Design Software

 

FUNCTION

GENOPLANTETMSPADS tries to design a unique GST (Gene-Sequence Tag) and a specific primer set for its PCR amplification, on a genomic sequence, knowing the gene structure.

 

DESCRIPTION

In transcriptome approaches, probes corresponding to individual members of multigenic families often lack specificity and are responsible of molecular cross-hybridization events. GENOPLANTETMSPADS uses a strategy to optimize the selection of specific probes or unique GST (Gene Sequence Tag) in a poor conserved region of each gene in a given genome. It can be applied when the genome considered is fully sequenced or if the gene family involved is exhaustively characterized. From a gene for which the intron-exon structure is known (or predicted), the program selects, by comparison with the whole genome, the less conserved region(s), and designs a primer pair specific to that region for its amplification from genomic DNA.


Access mode(s):

Web free access
Command line on saruman.versailles.inra.fr

Keyword(s):

primer PCR amplification genome sequence

Update: 23 Jan 2015
Creation date: 14 Oct 2011