Blast Analysis : gnl|CDD|211553

Analysis	rpsblast_cdd	Start	22
End	273	Strand	+
Length	252	Note	Gaps:66
Hit Coverage	55.65	Hit Length	460
Hit Pident	25.00	E Val	3e-08
Hit Description	TIGR00132 gatA aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase A subunit. In many species Gln--tRNA ligase is missing. tRNA(Gln) is misacylated with Glu after which a heterotrimeric amidotransferase converts Glu to Gln. This model represents the amidase chain of that heterotrimer encoded by the gatA gene. In the Archaea Asn--tRNA ligase is also missing. This amidase subunit may also function in the conversion of Asp-tRNA(Asn) to Asn-tRNA(Asn) presumably with a different recognition unit to replace gatB. Both Methanococcus jannaschii and Methanobacterium thermoautotrophicum have both authentic gatB and a gatB-related gene but only one gene like gatA. It has been shown that gatA can be expressed only when gatC is also expressed. In most species expressing the amidotransferase the gatC ortholog is about 90 residues in length but in Mycoplasma genitalium and Mycoplasma pneumoniae the gatC equivalent is as the C-terminal domain of a much longer protein. Not surprisingly the Mycoplasmas also represent the most atypical lineage of gatA orthology. This orthology group is more narrowly defined here than in Proc Natl Acad Aci USA 94 11819-11826 (1997). In particular a Rhodococcus homolog found in association with nitrile hydratase genes and described as an enantiomer-selective amidase active on several 2-aryl propionamides is excluded here. It is likely however that the amidase subunit GatA is not exclusively a part of the Glu-tRNA(Gln) amidotransferase heterotrimer and restricted to that function in all species.	Hit Pcons	18.36
Name	Qrob_P0274740.2

1 Protein

Protein Identifier	Organism . Name	Score	Score Type	Protein Description	Alias (in v1)	Code Enzyme	Gene Prediction Quality
Qrob_P0274740.2	Quercus robur	94.0	egn	(M=30) K01426 - amidase [EC:3.5.1.4]			validated

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