Protein : Qrob_P0274740.2 Q. robur
Protein Identifier  ? Qrob_P0274740.2 Organism . Name  Quercus robur
Score  94.0 Score Type  egn
Protein Description  (M=30) K01426 - amidase [EC:3.5.1.4] Gene Prediction Quality  validated
Protein length 

Sequence

Length: 286  
Kegg Orthology  K01426
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0 Synonyms

1 GO Terms

Identifier Name Description
GO:0016884 carbon-nitrogen ligase activity, with glutamine as amido-N-donor Catalysis of the transfer of the amide nitrogen of glutamine to a variety of substrates. GATases catalyze two separate reactions at two active sites, which are located either on a single polypeptide chain or on different subunits. In the glutaminase reaction, glutamine is hydrolyzed to glutamate and ammonia, which is added to an acceptor substrate in the synthase reaction.

26 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|vvi:100245248 20 285 + 266 Gaps:7 51.17 514 72.24 8e-123 putative amidase C869.01-like
blastp_kegg lcl|vvi:100246978 19 285 + 267 Gaps:9 50.99 506 72.09 2e-121 putative amidase C869.01-like
blastp_kegg lcl|vvi:100252118 20 285 + 266 Gaps:9 49.90 515 72.37 2e-119 putative amidase C869.01-like
blastp_kegg lcl|pop:POPTR_0009s13320g 20 284 + 265 Gaps:7 49.43 522 68.99 1e-117 POPTRDRAFT_876197 hypothetical protein
blastp_kegg lcl|cit:102630985 20 285 + 266 Gaps:6 50.38 520 67.56 1e-117 putative amidase C869.01-like
blastp_kegg lcl|cic:CICLE_v10028286mg 20 284 + 265 Gaps:5 52.74 493 66.15 2e-116 hypothetical protein
blastp_kegg lcl|cit:102625290 21 284 + 264 Gaps:5 49.81 520 65.25 1e-114 putative amidase C869.01-like
blastp_kegg lcl|fve:101302724 20 285 + 266 Gaps:6 50.48 519 67.18 3e-114 putative amidase C869.01-like
blastp_kegg lcl|pper:PRUPE_ppa004274mg 21 285 + 265 Gaps:6 50.29 519 66.67 3e-112 hypothetical protein
blastp_kegg lcl|pop:POPTR_0004s17680g 20 284 + 265 Gaps:7 50.98 510 67.69 3e-112 POPTRDRAFT_556603 amidase family protein
blastp_pdb 1m22_B 21 283 + 263 Gaps:23 52.49 503 35.23 2e-33 mol:protein length:503 peptide amidase
blastp_pdb 1m22_A 21 283 + 263 Gaps:23 52.49 503 35.23 2e-33 mol:protein length:503 peptide amidase
blastp_pdb 1m21_B 21 283 + 263 Gaps:23 52.49 503 35.23 2e-33 mol:protein length:503 Peptide Amidase
blastp_pdb 1m21_A 21 283 + 263 Gaps:23 52.49 503 35.23 2e-33 mol:protein length:503 Peptide Amidase
blastp_uniprot_sprot sp|Q9URY4|YI01_SCHPO 22 283 + 262 Gaps:26 49.06 583 26.57 2e-19 Putative amidase C869.01 OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN SPAC869.01 PE 3 SV 1
blastp_uniprot_sprot sp|Q7VCJ0|GATA_PROMA 23 277 + 255 Gaps:42 53.70 486 25.67 2e-06 Glutamyl-tRNA(Gln) amidotransferase subunit A OS Prochlorococcus marinus (strain SARG / CCMP1375 / SS120) GN gatA PE 3 SV 1
blastp_uniprot_sprot sp|A9B9W7|GATA_PROM4 22 277 + 256 Gaps:38 53.80 487 25.57 4e-06 Glutamyl-tRNA(Gln) amidotransferase subunit A OS Prochlorococcus marinus (strain MIT 9211) GN gatA PE 3 SV 1
rpsblast_cdd gnl|CDD|181247 22 285 + 264 Gaps:25 53.72 497 35.96 1e-46 PRK08137 PRK08137 amidase Provisional.
rpsblast_cdd gnl|CDD|180715 22 282 + 261 Gaps:24 51.53 491 32.81 1e-38 PRK06828 PRK06828 amidase Provisional.
rpsblast_cdd gnl|CDD|180665 22 282 + 261 Gaps:17 45.52 536 30.74 8e-30 PRK06707 PRK06707 amidase Provisional.
rpsblast_cdd gnl|CDD|30503 22 281 + 260 Gaps:46 53.05 475 21.83 2e-15 COG0154 GatA Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases [Translation ribosomal structure and biogenesis].
rpsblast_cdd gnl|CDD|183377 59 284 + 226 Gaps:15 34.63 615 29.11 2e-13 PRK11910 PRK11910 amidase Provisional.
rpsblast_cdd gnl|CDD|201788 22 265 + 244 Gaps:47 54.99 431 27.43 6e-10 pfam01425 Amidase Amidase.
rpsblast_cdd gnl|CDD|180998 64 282 + 219 Gaps:37 48.31 472 24.56 2e-08 PRK07488 PRK07488 indole acetimide hydrolase Validated.
rpsblast_cdd gnl|CDD|211553 22 273 + 252 Gaps:66 55.65 460 25.00 3e-08 TIGR00132 gatA aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase A subunit. In many species Gln--tRNA ligase is missing. tRNA(Gln) is misacylated with Glu after which a heterotrimeric amidotransferase converts Glu to Gln. This model represents the amidase chain of that heterotrimer encoded by the gatA gene. In the Archaea Asn--tRNA ligase is also missing. This amidase subunit may also function in the conversion of Asp-tRNA(Asn) to Asn-tRNA(Asn) presumably with a different recognition unit to replace gatB. Both Methanococcus jannaschii and Methanobacterium thermoautotrophicum have both authentic gatB and a gatB-related gene but only one gene like gatA. It has been shown that gatA can be expressed only when gatC is also expressed. In most species expressing the amidotransferase the gatC ortholog is about 90 residues in length but in Mycoplasma genitalium and Mycoplasma pneumoniae the gatC equivalent is as the C-terminal domain of a much longer protein. Not surprisingly the Mycoplasmas also represent the most atypical lineage of gatA orthology. This orthology group is more narrowly defined here than in Proc Natl Acad Aci USA 94 11819-11826 (1997). In particular a Rhodococcus homolog found in association with nitrile hydratase genes and described as an enantiomer-selective amidase active on several 2-aryl propionamides is excluded here. It is likely however that the amidase subunit GatA is not exclusively a part of the Glu-tRNA(Gln) amidotransferase heterotrimer and restricted to that function in all species.
rpsblast_kog gnl|CDD|36425 22 285 + 264 Gaps:25 54.35 506 21.45 2e-22 KOG1211 KOG1211 KOG1211 Amidases [Translation ribosomal structure and biogenesis].

5 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
SUPERFAMILY 22 282 261 SSF75304 "KEGG:00970+6.3.5.7","MetaCyc:PWY-5921" none IPR023631
Pfam 22 264 243 PF01425 "KEGG:00970+6.3.5.7","MetaCyc:PWY-5921" Amidase IPR000120
PANTHER 22 285 264 PTHR11895:SF8 none none none
PANTHER 22 285 264 PTHR11895 "KEGG:00970+6.3.5.7","MetaCyc:PWY-5921";signature_desc=AMIDASE none IPR000120
Gene3D 22 284 263 G3DSA:3.90.1300.10 "KEGG:00970+6.3.5.7","MetaCyc:PWY-5921" none IPR023631

0 Localization

4 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Bourran2_2015_nEpiBC_3P Qrob_Chr12 12 s_1B73S5_217 v_7050_211 28,31 26,37 28,45 lod 4.5 11.6
Bourran2_2015_nP_3P Qrob_Chr12 12 v_10140_295 v_838_303 16 14,69 18,01 lod 5.1 13.7
Bourran2_2002_QTL17_peak_Bud_burst_A4 Qrob_Chr12 12 s_1CTJ3J_556 s_1CTJ3J_556 0 0 25 lod 2,9 6
Bourran2_2014_nSeqBC_3P Qrob_Chr12 12 s_1EO8V5_710 s_1A2VMU_355 11,04 0 32,58 lod 1,7196 4,7

0 Targeting