| Analysis | rpsblast_cdd | Start | 7 |
| End | 72 | Strand | + |
| Length | 66 | Note | Gaps:1 |
| Hit Coverage | 17.06 | Hit Length | 381 |
| Hit Pident | 36.92 | E Val | 3e-16 |
| Hit Description | cd00186 TOP1Ac DNA Topoisomerase subtype IA DNA-binding ATP-binding and catalytic domain of bacterial DNA topoisomerases I and III and eukaryotic DNA topoisomerase III and eubacterial and archael reverse gyrases. Topoisomerases clevage single or double stranded DNA and then rejoin the broken phosphodiester backbone. Proposed catalytic mechanism of single stranded DNA cleavage is by phosphoryl transfer through a tyrosine nucleophile using acid/base catalysis. Tyr is activated by a nearby group (not yet identified) acting as a general base for nucleophilic attack on the 5' phosphate of the scissile bond. Arg and Lys stabilize the pentavalent transition state. Glu then acts as a proton donor for the leaving 3'-oxygen upon cleavage of the scissile strand.. | Hit Pcons | 15.38 |
| Name | Qrob_P0189210.2 |
| Protein Identifier |
Organism . Name |
Score | Score Type | Protein Description | Alias (in v1) | Code Enzyme | Gene Prediction Quality |
|---|---|---|---|---|---|---|---|
| Qrob_P0189210.2 | Quercus robur | 67.3 | egn | (M=4) PTHR11390:SF23 - DNA TOPOISOMERASE I-RELATED | EC:5.99.1.2 | validated |
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