| Analysis | rpsblast_cdd | Start | 7 |
| End | 89 | Strand | + |
| Length | 83 | Note | Gaps:1 |
| Hit Coverage | 100.00 | Hit Length | 84 |
| Hit Pident | 52.38 | E Val | 1e-31 |
| Hit Description | pfam04683 Proteasom_Rpn13 Proteasome complex subunit Rpn13 ubiquitin receptor. This family was thought originally to be involved in cell-adhesion but the members are now known to be proteasome subunit Rpn13 a novel ubiquitin receptor. The 26S proteasome is a huge macromolecular protein-degradation machine consisting of a proteolytically active 20S core in the form of four disc-like proteins and one or two 19S regulatory particles. The regulatory particle(s) sit on the top and or bottom of the core de-ubiquitinate the substrate peptides unfold them and guide them into the narrow channel through the centre of the core. Rpn13 and its homologues dock onto the regulatory particle through the N-terminal region which binds Rpn2. The C-terminal part of the domain binds de-ubiquitinating enzyme Uch37/UCHL5 and enhances its isopeptidase activity. Rpn13 binds ubiquitin via a conserved amino-terminal region called the pleckstrin-like receptor for ubiquitin termed Pru domain. The domain forms two contiguous anti-parallel beta-sheets with a configuration similar to the pleckstrin-homology domain (PHD) fold. Rpn13's ability to bind ubiquitin and the proteasome subunit Rpn2/S1 simultaneously supports evidence of its role as a ubiquitin receptor. Finally when complexed to di-ubiquitin via the Pru and Uch37 via the C-terminal part it frees up the distal ubiquitin for de-ubiquitination by the Uch37. | Hit Pcons | 20.24 |
| Name | Qrob_P0026480.2 |
| Protein Identifier |
Organism . Name |
Score | Score Type | Protein Description | Alias (in v1) | Code Enzyme | Gene Prediction Quality |
|---|---|---|---|---|---|---|---|
| Qrob_P0026480.2 | Quercus robur | 100.0 | egn | (M=1) PTHR12225 - ADHESION REGULATING MOLECULE 1 (110 KDA CELL MEMBRANE GLYCOPROTEIN) | validated |
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