Protein : Qrob_P0641340.2 Q. robur
Protein Identifier  ? Qrob_P0641340.2 Organism . Name  Quercus robur
Score  95.1 Score Type  egn
Protein Description  (M=2) K01870 - isoleucyl-tRNA synthetase [EC:6.1.1.5] Code Enzyme  EC:6.1.1.5
Gene Prediction Quality  validated Protein length 

Sequence

Length: 345  
Kegg Orthology  K01870
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0 Synonyms

8 GO Terms

Identifier Name Description
GO:0005524 ATP binding Interacting selectively and non-covalently with ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
GO:0005737 cytoplasm All of the contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
GO:0000166 nucleotide binding Interacting selectively and non-covalently with a nucleotide, any compound consisting of a nucleoside that is esterified with (ortho)phosphate or an oligophosphate at any hydroxyl group on the ribose or deoxyribose.
GO:0004812 aminoacyl-tRNA ligase activity Catalysis of the formation of aminoacyl-tRNA from ATP, amino acid, and tRNA with the release of diphosphate and AMP.
GO:0006418 tRNA aminoacylation for protein translation The synthesis of aminoacyl tRNA by the formation of an ester bond between the 3'-hydroxyl group of the most 3' adenosine of the tRNA, to be used in ribosome-mediated polypeptide synthesis.
GO:0002161 aminoacyl-tRNA editing activity The hydrolysis of an incorrectly aminoacylated tRNA.
GO:0004822 isoleucine-tRNA ligase activity Catalysis of the reaction: L-isoleucine + ATP + tRNA(Ile) = L-isoleucyl-tRNA(Ile) + AMP + diphosphate + 2 H(+).
GO:0006428 isoleucyl-tRNA aminoacylation The process of coupling isoleucine to isoleucyl-tRNA, catalyzed by isoleucyl-tRNA synthetase. In tRNA aminoacylation, the amino acid is first activated by linkage to AMP and then transferred to either the 2'- or the 3'-hydroxyl group of the 3'-adenosine residue of the tRNA.

45 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|mdm:103452907 1 331 + 331 Gaps:4 30.10 1093 81.46 0.0 isoleucine--tRNA ligase mitochondrial-like
blastp_kegg lcl|mdm:103403761 1 331 + 331 Gaps:4 30.10 1093 81.16 0.0 isoleucine--tRNA ligase mitochondrial-like
blastp_kegg lcl|pper:PRUPE_ppa000564mg 1 331 + 331 Gaps:1 30.32 1095 81.02 0.0 hypothetical protein
blastp_kegg lcl|pmum:103323008 1 331 + 331 Gaps:4 30.13 1092 81.46 0.0 isoleucine--tRNA ligase mitochondrial
blastp_kegg lcl|pxb:103943653 1 331 + 331 Gaps:4 30.10 1093 81.16 0.0 isoleucine--tRNA ligase mitochondrial
blastp_kegg lcl|tcc:TCM_009231 1 331 + 331 Gaps:4 30.10 1093 79.33 0.0 TRNA synthetase class I (I L M and V) family protein isoform 1
blastp_kegg lcl|cam:101515155 3 335 + 333 Gaps:4 30.46 1080 77.51 1e-179 isoleucine--tRNA ligase-like
blastp_kegg lcl|pvu:PHAVU_003G105600g 3 335 + 333 Gaps:4 30.07 1094 75.99 7e-176 hypothetical protein
blastp_kegg lcl|cit:102618088 1 331 + 331 Gaps:4 30.02 1096 78.42 1e-175 probable isoleucine--tRNA ligase mitochondrial-like
blastp_kegg lcl|gmx:100783252 2 335 + 334 Gaps:4 30.19 1093 75.76 3e-175 probable isoleucine--tRNA ligase mitochondrial-like
blastp_pdb 1qu3_A 84 335 + 252 Gaps:20 26.17 917 54.17 3e-74 mol:protein length:917 ISOLEUCYL-TRNA SYNTHETASE
blastp_pdb 1qu2_A 84 335 + 252 Gaps:20 26.17 917 54.17 3e-74 mol:protein length:917 ISOLEUCYL-TRNA SYNTHETASE
blastp_pdb 1ffy_A 84 335 + 252 Gaps:20 26.17 917 54.17 3e-74 mol:protein length:917 ISOLEUCYL-TRNA SYNTHETASE
blastp_pdb 1jzs_A 105 335 + 231 Gaps:17 27.53 821 32.30 3e-24 mol:protein length:821 Isoleucyl-tRNA synthetase
blastp_pdb 1jzq_A 105 335 + 231 Gaps:17 27.53 821 32.30 3e-24 mol:protein length:821 Isoleucyl-tRNA synthetase
blastp_pdb 1ile_A 105 335 + 231 Gaps:17 27.53 821 32.30 3e-24 mol:protein length:821 ISOLEUCYL-TRNA SYNTHETASE
blastp_pdb 1iyw_B 124 284 + 161 Gaps:1 18.56 862 27.50 3e-16 mol:protein length:862 Valyl-tRNA Synthetase
blastp_pdb 1iyw_A 124 284 + 161 Gaps:1 18.56 862 27.50 3e-16 mol:protein length:862 Valyl-tRNA Synthetase
blastp_pdb 1ivs_B 124 284 + 161 Gaps:1 18.56 862 27.50 3e-16 mol:protein length:862 Valyl-tRNA synthetase
blastp_pdb 1ivs_A 124 284 + 161 Gaps:1 18.56 862 27.50 3e-16 mol:protein length:862 Valyl-tRNA synthetase
blastp_uniprot_sprot sp|Q8YXY3|SYI_NOSS1 80 333 + 254 Gaps:3 26.15 960 68.13 1e-117 Isoleucine--tRNA ligase OS Nostoc sp. (strain PCC 7120 / UTEX 2576) GN ileS PE 3 SV 1
blastp_uniprot_sprot sp|B2IZP5|SYI_NOSP7 80 333 + 254 Gaps:3 26.17 959 67.33 3e-116 Isoleucine--tRNA ligase OS Nostoc punctiforme (strain ATCC 29133 / PCC 73102) GN ileS PE 3 SV 1
blastp_uniprot_sprot sp|B8HMX3|SYI_CYAP4 80 333 + 254 Gaps:3 26.28 955 67.33 5e-116 Isoleucine--tRNA ligase OS Cyanothece sp. (strain PCC 7425 / ATCC 29141) GN ileS PE 3 SV 1
blastp_uniprot_sprot sp|B1WVA3|SYI_CYAA5 84 333 + 250 Gaps:3 25.76 959 68.02 8e-115 Isoleucine--tRNA ligase OS Cyanothece sp. (strain ATCC 51142) GN ileS PE 3 SV 1
blastp_uniprot_sprot sp|B0JSP4|SYI_MICAN 84 333 + 250 Gaps:6 25.63 952 69.26 9e-115 Isoleucine--tRNA ligase OS Microcystis aeruginosa (strain NIES-843) GN ileS PE 3 SV 1
blastp_uniprot_sprot sp|B7KFT1|SYI_CYAP7 80 333 + 254 Gaps:3 26.17 959 67.33 4e-114 Isoleucine--tRNA ligase OS Cyanothece sp. (strain PCC 7424) GN ileS PE 3 SV 1
blastp_uniprot_sprot sp|B7K5H5|SYI_CYAP8 80 333 + 254 Gaps:3 26.17 959 66.53 2e-113 Isoleucine--tRNA ligase OS Cyanothece sp. (strain PCC 8801) GN ileS PE 3 SV 1
blastp_uniprot_sprot sp|B0C872|SYI_ACAM1 80 333 + 254 Gaps:3 25.85 971 66.14 2e-113 Isoleucine--tRNA ligase OS Acaryochloris marina (strain MBIC 11017) GN ileS PE 3 SV 1
blastp_uniprot_sprot sp|Q5N1G1|SYI_SYNP6 80 333 + 254 Gaps:4 26.21 954 65.60 2e-108 Isoleucine--tRNA ligase OS Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) GN ileS PE 3 SV 1
blastp_uniprot_sprot sp|P73505|SYI_SYNY3 80 333 + 254 Gaps:3 25.40 988 64.14 2e-107 Isoleucine--tRNA ligase OS Synechocystis sp. (strain PCC 6803 / Kazusa) GN ileS PE 3 SV 1

10 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
PANTHER 54 333 280 PTHR11946:SF9 "KEGG:00970+6.1.1.5";signature_desc=ISOLEUCYL TRNA SYNTHETASE none IPR023585
SUPERFAMILY 289 333 45 SSF50677 "Reactome:REACT_71" none IPR009008
PRINTS 130 141 12 PR00984 "KEGG:00970+6.1.1.5","Reactome:REACT_71" Isoleucyl-tRNA synthetase signature IPR002301
PRINTS 321 344 24 PR00984 "KEGG:00970+6.1.1.5","Reactome:REACT_71" Isoleucyl-tRNA synthetase signature IPR002301
SUPERFAMILY 92 287 196 SSF52374 none none none
Pfam 108 334 227 PF00133 "Reactome:REACT_71" tRNA synthetases class I (I, L, M and V) IPR002300
Gene3D 94 274 181 G3DSA:3.40.50.620 none none IPR014729
PANTHER 54 333 280 PTHR11946 none none none
Gene3D 275 333 59 G3DSA:3.90.740.10 "Reactome:REACT_71" none IPR009008
ProSitePatterns 137 148 12 PS00178 "Reactome:REACT_71" Aminoacyl-transfer RNA synthetases class-I signature. IPR001412

0 Localization

17 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Bourran2_2014_nSecLBD_3P Qrob_Chr08 8 s_1BN2OD_551 s_1B5AYF_599 17,17 0 43,51 lod 1,9229 4,4
Bourran2_2014_nLBD*_3P Qrob_Chr08 8 v_5216_549 v_11837_70 12,25 0 35,55 lod 2,5951 6
Bourran2_2014_nP*_3P Qrob_Chr08 8 v_5216_549 v_11837_70 12,19 0 31,97 lod 2,8472 6
Bourran2_2002_QTL11_peak_Bud_burst_A4 Qrob_Chr06 6 s_1C41PA_791 s_1AM1AV_1141 19,17 0 34,57 lod 2,3 2,9
Bourran2_2014_aSeqBC_3P Qrob_Chr06 6 v_506_189 v_686_77 30,72 13,58 43,48 lod 2,2746 6,3
Bourran2_2014_aSeqBC*_A4 Qrob_Chr06 6 s_2F5MK3_712 v_444_355 27,13 14,86 39,46 lod 3,7847 9,8
Bourran2_2014_nEpis*_3P Qrob_Chr08 8 s_1DA4QW_688 s_1DNI7D_820 17,96 0 37,75 lod 2,9745 7,5
Bourran2_2014_nEpis*_A4 Qrob_Chr07 7 v_12400_446 s_1BPEBU_1211 6,93 0 15,13 lod 4,7411 11
Bourran2_2014_nFork*_3P Qrob_Chr06 6 v_12930_125 s_1AMZEI_909 34,28 7,43 41,48 lod 2,4044 5,5
Bourran2_2014_nLBD_A4 Qrob_Chr06 6 v_12930_125 s_1AMZEI_909 37,41 9,8 50,1 lod 1,9524 4,1
Bourran2_2014_nPriBD_A4 Qrob_Chr06 6 v_12930_125 s_1AMZEI_909 34,51 3,36 51,86 lod 1,6747 3,9
Bourran2_2014_nPriLBD*_3P Qrob_Chr11 11 v_12066_307 s_1B15GJ_447 3,37 0,57 16,17 lod 3,4299 6,9
Bourran2_2014_nSecLBD_A4 Qrob_Chr07 7 v_8327_222 s_1A4WGY_363 16,04 0 44,69 lod 2,6373 6,5
Bourran2_2014_vEpiBC_A4 Qrob_Chr06 6 v_12930_125 s_1AMZEI_909 37,55 14,41 50,01 lod 1,7882 4,8
Champenoux_2015_nEpis_3P Qrob_Chr11 11 s_1DG9PM_867 s_1BZ083_1312 26,53 25,47 27,72 lod 4.4 8.9
Champenoux_2015_nP_3P Qrob_Chr06 6 s_1A386O_228 s_1AYZFS_603 27,03 26,47 27,34 lod 2.8 7.2
Bourran2_2014_nPriBD*_3P Qrob_Chr06 6 s_1B6WLL_980 v_10064_133 11,49 0 31,52 lod 4,1208 9,1

0 Targeting