Protein : Qrob_P0596850.2 Q. robur

Protein Identifier  ? Qrob_P0596850.2 Organism . Name  Quercus robur
Score  100.0 Score Type  egn
Protein Description  (M=6) 3.1.3.12 - Trehalose-phosphatase. Code Enzyme  EC:3.1.3.12
Gene Prediction Quality  validated Protein length 

Sequence

Length: 219  

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0 Synonyms

2 GO Terms

Identifier Name Description
GO:0003824 catalytic activity Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic.
GO:0005992 trehalose biosynthetic process The chemical reactions and pathways resulting in the formation of trehalose, a disaccharide isomeric with sucrose and obtained from certain lichens and fungi.

29 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|rcu:RCOM_0198410 1 95 + 95 none 25.47 373 76.84 2e-42 trehalose-6-phosphate synthase putative (EC:3.1.3.12)
blastp_kegg lcl|cam:101503089 1 95 + 95 none 26.10 364 76.84 2e-41 probable trehalose-phosphate phosphatase J-like
blastp_kegg lcl|fve:101301240 1 95 + 95 none 25.20 377 74.74 3e-41 probable trehalose-phosphate phosphatase J-like
blastp_kegg lcl|gmx:100794430 1 95 + 95 none 26.17 363 74.74 5e-41 probable trehalose-phosphate phosphatase J-like
blastp_kegg lcl|pvu:PHAVU_001G251300g 1 95 + 95 none 25.89 367 73.68 8e-41 hypothetical protein
blastp_kegg lcl|tcc:TCM_001144 1 95 + 95 none 25.33 375 75.79 1e-40 Haloacid dehalogenase-like hydrolase (HAD) superfamily protein
blastp_kegg lcl|gmx:100815357 1 95 + 95 Gaps:1 25.75 365 76.60 1e-40 probable trehalose-phosphate phosphatase J-like
blastp_kegg lcl|pper:PRUPE_ppa007221mg 1 95 + 95 none 25.20 377 72.63 1e-40 hypothetical protein
blastp_kegg lcl|pop:POPTR_0005s07890g 1 95 + 95 none 25.47 373 73.68 2e-40 POPTRDRAFT_650738 trehalose-6-phosphate phosphatase family protein
blastp_kegg lcl|pop:POPTR_0007s05670g 1 95 + 95 none 25.40 374 73.68 2e-40 POPTRDRAFT_654797 trehalose-6-phosphate phosphatase family protein
blastp_pdb 1u02_A 46 100 + 55 none 23.01 239 38.18 5e-06 mol:protein length:239 trehalose-6-phosphate phosphatase related pro
blastp_uniprot_sprot sp|F4KFG5|TPPI_ARATH 1 95 + 95 Gaps:3 26.56 369 72.45 4e-40 Probable trehalose-phosphate phosphatase I OS Arabidopsis thaliana GN TPPI PE 2 SV 1
blastp_uniprot_sprot sp|Q5HZ05|TPPJ_ARATH 1 95 + 95 Gaps:2 26.22 370 67.01 7e-35 Probable trehalose-phosphate phosphatase J OS Arabidopsis thaliana GN TPPJ PE 1 SV 1
blastp_uniprot_sprot sp|Q6ZAL2|TPP6_ORYSJ 1 95 + 95 Gaps:6 27.30 370 66.34 2e-34 Probable trehalose-phosphate phosphatase 6 OS Oryza sativa subsp. japonica GN TPP6 PE 2 SV 1
blastp_uniprot_sprot sp|Q8GWG2|TPPH_ARATH 1 95 + 95 Gaps:4 26.07 349 68.13 8e-34 Probable trehalose-phosphate phosphatase H OS Arabidopsis thaliana GN TPPH PE 2 SV 1
blastp_uniprot_sprot sp|Q67X99|TPPE_ARATH 1 96 + 96 Gaps:4 25.99 354 68.48 3e-33 Probable trehalose-phosphate phosphatase E OS Arabidopsis thaliana GN TPPE PE 2 SV 1
blastp_uniprot_sprot sp|Q67XC9|TPPD_ARATH 1 95 + 95 Gaps:7 27.64 369 61.76 6e-33 Probable trehalose-phosphate phosphatase D OS Arabidopsis thaliana GN TPPD PE 2 SV 1
blastp_uniprot_sprot sp|Q6H5L4|TPP7_ORYSJ 1 95 + 95 Gaps:4 26.40 375 63.64 1e-32 Probable trehalose-phosphate phosphatase 7 OS Oryza sativa subsp. japonica GN TPP7 PE 2 SV 2
blastp_uniprot_sprot sp|Q6ZGP8|TPP4_ORYSJ 1 95 + 95 none 25.89 367 58.95 6e-29 Probable trehalose-phosphate phosphatase 4 OS Oryza sativa subsp. japonica GN TPP4 PE 2 SV 1
blastp_uniprot_sprot sp|Q9C9S4|TPPB_ARATH 1 95 + 95 Gaps:4 26.47 374 54.55 2e-27 Trehalose-phosphate phosphatase B OS Arabidopsis thaliana GN TPPB PE 1 SV 1
blastp_uniprot_sprot sp|Q10KF5|TPP9_ORYSJ 1 95 + 95 Gaps:5 26.74 374 57.00 2e-26 Probable trehalose-phosphate phosphatase 9 OS Oryza sativa subsp. japonica GN TPP9 PE 3 SV 1
rpsblast_cdd gnl|CDD|178591 1 95 + 95 Gaps:3 26.78 366 71.43 8e-39 PLN03017 PLN03017 trehalose-phosphatase.
rpsblast_cdd gnl|CDD|177812 1 96 + 96 Gaps:4 25.99 354 68.48 6e-34 PLN02151 PLN02151 trehalose-phosphatase.
rpsblast_cdd gnl|CDD|178192 1 95 + 95 Gaps:9 27.08 384 54.81 9e-34 PLN02580 PLN02580 trehalose-phosphatase.
rpsblast_cdd gnl|CDD|145482 48 95 + 48 Gaps:2 21.28 235 50.00 8e-12 pfam02358 Trehalose_PPase Trehalose-phosphatase. This family consist of trehalose-phosphatases EC:3.1.3.12 these enzyme catalyze the de-phosphorylation of trehalose-6-phosphate to trehalose and orthophosphate. The aligned region is present in trehalose-phosphatases and comprises the entire length of the protein it is also found in the C-terminus of trehalose-6-phosphate synthase EC:2.4.1.15 adjacent to the trehalose-6-phosphate synthase domain - pfam00982. It would appear that the two equivalent genes in the E. coli otsBA operon otsA the trehalose-6-phosphate synthase and otsB trehalose-phosphatase (this family) have undergone gene fusion in most eukaryotes. Trehalose is a common disaccharide of bacteria fungi and invertebrates that appears to play a major role in desiccation tolerance.
rpsblast_cdd gnl|CDD|184712 27 95 + 69 Gaps:3 9.92 726 36.11 9e-11 PRK14501 PRK14501 putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB Provisional.
rpsblast_cdd gnl|CDD|32061 27 96 + 70 Gaps:2 27.07 266 30.56 7e-07 COG1877 OtsB Trehalose-6-phosphatase [Carbohydrate transport and metabolism].
rpsblast_cdd gnl|CDD|161997 42 90 + 49 Gaps:2 20.90 244 45.10 1e-06 TIGR00685 T6PP trehalose-phosphatase. Trehalose a neutral disaccharide of two glucose residues is an important osmolyte for dessication and/or salt tolerance in a number of prokaryotic and eukaryotic species including E. coli Saccharomyces cerevisiae and Arabidopsis thaliana. Many bacteria also utilize trehalose in the synthesis of trehalolipids specialized cell wall constituents believed to be involved in the uptake of hydrophobic substances. Trehalose dimycolate (TDM cord factor) and related substances are important constituents of the mycobacterial waxy coat and responsible for various clinically important immunological interactions with host organism. This enzyme trehalose-phosphatase removes a phosphate group in the final step of trehalose biosynthesis. The trehalose-phosphatase from Saccharomyces cerevisiae is fused to the synthase. At least 18 distinct sequences from Arabidopsis have been identified roughly half of these are of the fungal type with a fused synthase and half are like the bacterial members having only the phosphatase domain. It has been suggested that trehalose is being used in Arabidopsis as a regulatory molecule in development and possibly other processes.
rpsblast_kog gnl|CDD|36268 24 152 + 129 Gaps:12 17.90 732 26.72 8e-19 KOG1050 KOG1050 KOG1050 Trehalose-6-phosphate synthase component TPS1 and related subunits [Carbohydrate transport and metabolism].

10 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
Phobius 158 175 18 TRANSMEMBRANE none Region of a membrane-bound protein predicted to be embedded in the membrane. none
Pfam 48 97 50 PF02358 none Trehalose-phosphatase IPR003337
SUPERFAMILY 46 96 51 SSF56784 none none IPR023214
Gene3D 44 97 54 G3DSA:3.40.50.1000 none none IPR023214
PANTHER 1 94 94 PTHR10788:SF19 none none none
PANTHER 1 94 94 PTHR10788 none none none
Phobius 176 218 43 CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. none
Phobius 1 118 118 CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. none
Phobius 139 157 19 NON_CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. none
Phobius 119 138 20 TRANSMEMBRANE none Region of a membrane-bound protein predicted to be embedded in the membrane. none

0 Localization

0 Qtllist

0 Targeting