Protein : Qrob_P0509210.2 Q. robur

Protein Identifier  ? Qrob_P0509210.2 Organism . Name  Quercus robur
Score  88.1 Score Type  egn
Protein Description  (M=1) 2.3.3.9 - Malate synthase. Code Enzyme  EC:2.3.3.9
Gene Prediction Quality  validated Protein length 

Sequence

Length: 569  
Kegg Orthology  K01638

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0 Synonyms

3 GO Terms

Identifier Name Description
GO:0003824 catalytic activity Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic.
GO:0004474 malate synthase activity Catalysis of the reaction: acetyl-CoA + glyoxylate + H(2)O = (S)-malate + CoA + H(+).
GO:0006097 glyoxylate cycle A modification of the TCA cycle occurring in some plants and microorganisms, in which isocitrate is cleaved to glyoxylate and succinate. Glyoxylate can then react with acetyl-CoA to form malate.

33 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|tcc:TCM_014591 1 568 + 568 Gaps:1 100.00 567 89.42 0.0 Malate synthase glyoxysomal
blastp_kegg lcl|rcu:RCOM_1506900 1 568 + 568 Gaps:1 100.00 567 88.01 0.0 malate synthase putative (EC:2.3.3.9)
blastp_kegg lcl|vvi:100261216 1 568 + 568 none 100.00 568 87.15 0.0 malate synthase glyoxysomal-like
blastp_kegg lcl|mtr:MTR_077s0031 2 568 + 567 none 100.00 567 86.42 0.0 Malate synthase
blastp_kegg lcl|cic:CICLE_v10019499mg 1 568 + 568 Gaps:1 100.00 569 86.47 0.0 hypothetical protein
blastp_kegg lcl|cit:102626401 1 568 + 568 Gaps:1 100.00 569 85.94 0.0 malate synthase glyoxysomal-like
blastp_kegg lcl|pmum:103338030 1 568 + 568 Gaps:5 100.00 573 85.86 0.0 malate synthase glyoxysomal
blastp_kegg lcl|gmx:732628 2 568 + 567 none 100.00 567 85.36 0.0 MS malate synthase
blastp_kegg lcl|sly:101267103 1 568 + 568 Gaps:3 100.00 571 84.41 0.0 malate synthase glyoxysomal-like
blastp_kegg lcl|cam:101508718 6 568 + 563 none 99.12 568 85.26 0.0 malate synthase glyoxysomal-like
blastp_pdb 3cux_A 27 557 + 531 Gaps:9 98.86 528 54.02 0.0 mol:protein length:528 Malate synthase
blastp_pdb 3cv2_B 33 557 + 525 Gaps:9 96.99 532 50.39 1e-175 mol:protein length:532 Malate synthase A
blastp_pdb 3cv2_A 33 557 + 525 Gaps:9 96.99 532 50.39 1e-175 mol:protein length:532 Malate synthase A
blastp_pdb 3cv1_A 33 557 + 525 Gaps:9 96.99 532 50.39 1e-175 mol:protein length:532 Malate synthase A
blastp_pdb 3cuz_A 33 557 + 525 Gaps:9 96.99 532 50.39 1e-175 mol:protein length:532 Malate synthase A
blastp_uniprot_sprot sp|P17815|MASY_RICCO 2 568 + 567 Gaps:2 100.00 567 87.65 0.0 Malate synthase glyoxysomal OS Ricinus communis PE 2 SV 1
blastp_uniprot_sprot sp|P17432|MASY_GOSHI 1 567 + 567 Gaps:2 99.65 567 86.73 0.0 Malate synthase glyoxysomal OS Gossypium hirsutum PE 2 SV 1
blastp_uniprot_sprot sp|P45458|MASY_SOYBN 5 568 + 564 none 100.00 564 85.28 0.0 Malate synthase glyoxysomal (Fragment) OS Glycine max PE 2 SV 1
blastp_uniprot_sprot sp|P08216|MASY_CUCSA 1 568 + 568 none 100.00 568 85.04 0.0 Malate synthase glyoxysomal OS Cucumis sativus PE 2 SV 2
blastp_uniprot_sprot sp|P24571|MASY_CUCMA 1 568 + 568 Gaps:2 100.00 566 83.75 0.0 Malate synthase glyoxysomal OS Cucurbita maxima PE 1 SV 1
blastp_uniprot_sprot sp|P13244|MASY_BRANA 21 568 + 548 Gaps:1 97.50 561 84.10 0.0 Malate synthase glyoxysomal OS Brassica napus PE 2 SV 1
blastp_uniprot_sprot sp|P49081|MASY_MAIZE 21 565 + 545 Gaps:1 97.67 559 75.27 0.0 Malate synthase glyoxysomal OS Zea mays GN LIP PE 2 SV 1
blastp_uniprot_sprot sp|Q43827|MASY_RAPSA 21 568 + 548 Gaps:6 97.53 566 73.73 0.0 Malate synthase glyoxysomal OS Raphanus sativus GN MLS PE 2 SV 1
blastp_uniprot_sprot sp|P95329|MASY_MYXXD 26 560 + 535 Gaps:10 97.04 541 58.10 0.0 Malate synthase OS Myxococcus xanthus (strain DK 1622) GN mls PE 3 SV 2
blastp_uniprot_sprot sp|Q8T2K9|MASY_DICDI 26 558 + 533 Gaps:22 97.42 543 51.61 5e-180 Malate synthase OS Dictyostelium discoideum GN masA PE 2 SV 2
rpsblast_cdd gnl|CDD|178233 20 568 + 549 Gaps:2 99.27 551 80.99 0.0 PLN02626 PLN02626 malate synthase.
rpsblast_cdd gnl|CDD|181729 22 557 + 536 Gaps:9 99.25 531 57.31 0.0 PRK09255 PRK09255 malate synthase Validated.
rpsblast_cdd gnl|CDD|48433 40 558 + 519 Gaps:10 100.00 511 61.06 0.0 cd00727 malate_synt_A Malate synthase A (MSA) present in some bacteria plants and fungi. Prokaryotic MSAs tend to be monomeric whereas eukaryotic enzymes are homomultimers. In general malate synthase catalyzes the Claisen condensation of glyoxylate and acetyl-CoA to malyl-CoA which hydrolyzes to malate and CoA. This reaction is part of the glyoxylate cycle which allows certain organisms like plants and fungi to derive their carbon requirements from two-carbon compounds by bypassing the two carboxylation steps of the citric acid cycle..
rpsblast_cdd gnl|CDD|201704 27 558 + 532 Gaps:23 100.00 517 57.83 0.0 pfam01274 Malate_synthase Malate synthase.
rpsblast_cdd gnl|CDD|188132 40 557 + 518 Gaps:8 99.80 511 57.84 0.0 TIGR01344 malate_syn_A malate synthase A. This model represents plant malate synthase and one of two bacterial forms designated malate synthase A. The distantly related malate synthase G is described by a separate model. This enzyme and isocitrate lyase are the two characteristic enzymes of the glyoxylate shunt. The shunt enables the cell to use acetyl-CoA to generate increased levels of TCA cycle intermediates for biosynthetic pathways such as gluconeogenesis.

9 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
TIGRFAM 40 557 518 TIGR01344 "KEGG:00620+2.3.3.9","KEGG:00630+2.3.3.9","MetaCyc:PWY-6728","MetaCyc:PWY-6969","MetaCyc:PWY-7118","MetaCyc:PWY-7294","MetaCyc:PWY-7295","UniPathway:UPA00703" malate_syn_A: malate synthase A IPR006252
ProSitePatterns 286 301 16 PS00510 "KEGG:00620+2.3.3.9","KEGG:00630+2.3.3.9","MetaCyc:PWY-6728","MetaCyc:PWY-6969","MetaCyc:PWY-7118","MetaCyc:PWY-7294","MetaCyc:PWY-7295","UniPathway:UPA00703" Malate synthase signature. IPR019830
SUPERFAMILY 23 559 537 SSF51645 "KEGG:00620+2.3.3.9","KEGG:00630+2.3.3.9","MetaCyc:PWY-6728","MetaCyc:PWY-6969","MetaCyc:PWY-7118","MetaCyc:PWY-7294","MetaCyc:PWY-7295","UniPathway:UPA00703" none IPR011076
Gene3D 89 436 348 G3DSA:3.20.20.360 none none none
Pfam 28 557 530 PF01274 "KEGG:00620+2.3.3.9","KEGG:00630+2.3.3.9","MetaCyc:PWY-6728","MetaCyc:PWY-6969","MetaCyc:PWY-7118","MetaCyc:PWY-7294","MetaCyc:PWY-7295","UniPathway:UPA00703" Malate synthase IPR001465
PIRSF 19 559 541 PIRSF001363 "KEGG:00620+2.3.3.9","KEGG:00630+2.3.3.9","MetaCyc:PWY-6728","MetaCyc:PWY-6969","MetaCyc:PWY-7118","MetaCyc:PWY-7294","MetaCyc:PWY-7295","UniPathway:UPA00703" none IPR006252
PANTHER 24 558 535 PTHR21631 none none none
PANTHER 24 558 535 PTHR21631:SF16 none none none
Gene3D 437 551 115 G3DSA:1.20.1220.12 none none none

0 Localization

6 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Bourran2_2002_QTL13_peak_Bud_burst_3P Qrob_Chr10 10 s_1B1AG7_637 s_1A3A1N_709 19,44 0 49,44 lod 3 5,6
Bourran2_2002_QTL15_peak_Bud_burst_A4 Qrob_Chr10 10 v_7579_71 s_1ATZMJ_189 23,94 0 43,64 lod 3,7 5,4
Bourran2_2014_vSeqBC_3P Qrob_Chr10 10 s_1A6CK6_610 v_7092_29 4,28 0 23,27 lod 2,8619 7
Bourran2_2014_vSeqBC_A4 Qrob_Chr10 10 s_1BAM4E_1326 s_1C0HD0_1012 36,88 7,71 46,03 lod 2,6164 7,3
Champenoux_2015_nPriLBD_3P Qrob_Chr10 10 v_15000_157 v_15000_310 15,68 15,9 15,91 lod 2.4 5.5
PM_1999_QTL16_peak_Bud_burst_3P Qrob_Chr10 10 s_1B6YNI_505 s_1A1ZO_1322 7,48 0 31,48 lod 4 7,1

0 Targeting