Protein : Qrob_P0343710.2 Q. robur
Protein Identifier  ? Qrob_P0343710.2 Organism . Name  Quercus robur
Score  97.1 Score Type  egn
Protein Description  (M=1) K02685 - DNA primase large subunit [EC:2.7.7.-] Gene Prediction Quality  validated
Protein length 

Sequence

Length: 410  
Kegg Orthology  K02685
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0 Synonyms

2 GO Terms

Identifier Name Description
GO:0003896 DNA primase activity Catalysis of the synthesis of a short RNA primer on a DNA template, providing a free 3'-OH that can be extended by DNA-directed DNA polymerases. Catalyzed by a DNA-directed RNA polymerase that forms a complex with alpha DNA polymerase.
GO:0006269 DNA replication, synthesis of RNA primer The synthesis of a short RNA polymer, usually 4-15 nucleotides long, using one strand of unwound DNA as a template; the RNA then serves as a primer from which DNA polymerases extend synthesis.

30 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|vvi:100244238 1 407 + 407 Gaps:45 99.56 452 77.33 0.0 probable DNA primase large subunit-like
blastp_kegg lcl|pper:PRUPE_ppa022318mg 1 404 + 404 Gaps:26 100.00 428 79.44 0.0 hypothetical protein
blastp_kegg lcl|pmum:103322094 1 404 + 404 Gaps:44 98.89 451 76.23 0.0 probable DNA primase large subunit
blastp_kegg lcl|tcc:TCM_008656 1 404 + 404 Gaps:45 98.68 453 75.17 0.0 DNA primase large subunit family isoform 1
blastp_kegg lcl|mdm:103452171 1 404 + 404 Gaps:45 98.89 452 74.05 0.0 probable DNA primase large subunit
blastp_kegg lcl|cic:CICLE_v10031511mg 1 405 + 405 Gaps:46 99.12 453 74.61 0.0 hypothetical protein
blastp_kegg lcl|csv:101217274 1 404 + 404 Gaps:45 98.89 452 73.83 0.0 probable DNA primase large subunit-like
blastp_kegg lcl|pxb:103935114 1 404 + 404 Gaps:45 98.89 452 73.60 0.0 probable DNA primase large subunit
blastp_kegg lcl|cmo:103498852 1 404 + 404 Gaps:45 98.89 452 73.83 0.0 probable DNA primase large subunit
blastp_kegg lcl|pop:POPTR_0003s16770g 1 404 + 404 Gaps:50 100.00 452 73.89 0.0 POPTRDRAFT_712440 DNA primase large subunit family protein
blastp_pdb 3q36_B 217 403 + 187 Gaps:4 98.44 192 52.38 4e-63 mol:protein length:192 DNA primase large subunit
blastp_pdb 3q36_A 217 403 + 187 Gaps:4 98.44 192 52.38 4e-63 mol:protein length:192 DNA primase large subunit
blastp_pdb 3l9q_B 220 403 + 184 Gaps:4 95.38 195 52.69 6e-62 mol:protein length:195 DNA primase large subunit
blastp_pdb 3l9q_A 220 403 + 184 Gaps:4 95.38 195 52.69 6e-62 mol:protein length:195 DNA primase large subunit
blastp_pdb 3lgb_B 230 400 + 171 Gaps:10 91.24 194 42.94 2e-44 mol:protein length:194 DNA primase large subunit
blastp_pdb 3lgb_A 230 400 + 171 Gaps:10 91.24 194 42.94 2e-44 mol:protein length:194 DNA primase large subunit
blastp_uniprot_sprot sp|Q84WJ2|PRI2_ARATH 1 405 + 405 Gaps:46 98.90 454 69.04 0.0 Probable DNA primase large subunit OS Arabidopsis thaliana GN At1g67320 PE 2 SV 2
blastp_uniprot_sprot sp|P33610|PRI2_MOUSE 18 401 + 384 Gaps:55 85.35 505 39.44 1e-102 DNA primase large subunit OS Mus musculus GN Prim2 PE 1 SV 1
blastp_uniprot_sprot sp|O89044|PRI2_RAT 1 401 + 401 Gaps:58 87.77 507 38.65 2e-101 DNA primase large subunit OS Rattus norvegicus GN Prim2 PE 2 SV 1
blastp_uniprot_sprot sp|P49643|PRI2_HUMAN 19 401 + 383 Gaps:55 84.48 509 39.07 3e-99 DNA primase large subunit OS Homo sapiens GN PRIM2 PE 1 SV 2
blastp_uniprot_sprot sp|Q9VPH2|PRI2_DROME 55 404 + 350 Gaps:34 69.42 533 39.19 1e-86 DNA primase large subunit OS Drosophila melanogaster GN DNApol-alpha60 PE 1 SV 2
blastp_uniprot_sprot sp|O74761|PRI2_SCHPO 17 394 + 378 Gaps:63 93.03 459 38.17 7e-82 DNA primase large subunit OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN pri2 PE 1 SV 1
blastp_uniprot_sprot sp|P20457|PRI2_YEAST 19 398 + 380 Gaps:78 85.23 528 33.56 2e-72 DNA primase large subunit OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN PRI2 PE 1 SV 1
blastp_uniprot_sprot sp|Q8NIZ4|PRI2_NEUCR 19 397 + 379 Gaps:90 89.47 513 35.29 2e-69 Probable DNA primase large subunit OS Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) GN 5F3.150 PE 3 SV 1
blastp_uniprot_sprot sp|O02334|PRI2_CAEEL 10 406 + 397 Gaps:67 90.26 503 30.62 1e-60 DNA primase large subunit OS Caenorhabditis elegans GN pri-2 PE 3 SV 2
blastp_uniprot_sprot sp|Q55BM5|PRI2_DICDI 21 397 + 377 Gaps:57 89.36 470 31.90 1e-55 DNA primase large subunit OS Dictyostelium discoideum GN prim2 PE 3 SV 1
rpsblast_cdd gnl|CDD|143474 21 397 + 377 Gaps:47 100.00 390 50.00 1e-138 cd07322 PriL_PriS_Eukaryotic Eukaryotic core primase: Large subunit PriL. Primases synthesize the RNA primers required for DNA replication. Primases are grouped into two classes bacteria/bacteriophage and archaeal/eukaryotic. The proteins in the two classes differ in structure and the replication apparatus components. Archaeal/eukaryotic core primase is a heterodimeric enzyme consisting of a small catalytic subunit (PriS) and a large subunit (PriL). In eukaryotic organisms a heterotetrameric enzyme formed by DNA polymerase alpha the B subunit and two primase subunits has primase activity. Although the catalytic activity resides within PriS the PriL subunit is essential for primase function as disruption of the PriL gene in yeast is lethal. PriL is composed of two structural domains. Several functions have been proposed for PriL such as stabilization of the PriS involvement in synthesis initiation improvement of primase processivity determination of product size and transfer of the products to DNA polymerase alpha.
rpsblast_cdd gnl|CDD|112900 131 394 + 264 Gaps:57 98.16 217 50.70 1e-64 pfam04104 DNA_primase_lrg Eukaryotic and archaeal DNA primase large subunit. DNA primase is the polymerase that synthesises small RNA primers for the Okazaki fragments made during discontinuous DNA replication. DNA primase is a heterodimer of two subunits the small subunit Pri1 (48 kDa in yeast) and the large subunit Pri2 (58 kDa in the yeast S. cerevisiae). The large subunit of DNA primase forms interactions with the small subunit and the structure implicates that it is not directly involved in catalysis but plays roles in correctly positioning the primase/DNA complex and in the transfer of RNA to DNA polymerase.
rpsblast_cdd gnl|CDD|32401 68 318 + 251 Gaps:44 70.25 363 23.14 5e-29 COG2219 PRI2 Eukaryotic-type DNA primase large subunit [DNA replication recombination and repair].
rpsblast_kog gnl|CDD|37478 17 398 + 382 Gaps:56 90.11 475 39.25 3e-98 KOG2267 KOG2267 KOG2267 Eukaryotic-type DNA primase large subunit [Replication recombination and repair].

3 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
PANTHER 17 402 386 PTHR10537 none none none
Pfam 129 394 266 PF04104 none Eukaryotic and archaeal DNA primase, large subunit IPR007238
PANTHER 17 402 386 PTHR10537:SF3 none none none

0 Localization

3 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Bourran_2000_2002_QTL6_Delta.F Qrob_Chr03 3 s_2HYXJ2_207 s_1AUAXK_317 29.12 6,3 45,37 lod 3.1666 0.026
Bourran2_2014_nLBD_3P Qrob_Chr03 3 s_1ET7H8_604 s_1BYNB5_834 23,73 0 46,72 lod 1,7573 3,8
PM_1999_QTL14_peak_Bud_burst_3P Qrob_Chr03 3 s_2F0SI1_756 v_6056_735 11,78 3,78 50,78 lod 2,5 4,5

0 Targeting