Protein : Qrob_P0332710.2 Q. robur

Protein Identifier  ? Qrob_P0332710.2 Organism . Name  Quercus robur
Score  28.0 Score Type  egn
Protein Description  (M=1) KOG0132//KOG0260//KOG0307//KOG0916//KOG1049//KOG1798//KOG1830//KOG1984//KOG1985//KOG3671 - RNA polymerase II C-terminal domain-binding protein RA4 contains RPR and RRM domains [RNA processing and modification Transcription]. // RNA polymerase II large subunit [Transcription]. // Vesicle coat complex COPII subunit SEC31 [Intracellular trafficking secretion and vesicular transport]. // 1 3-beta-glucan synthase/callose synthase catalytic subunit [Cell wall/membrane/envelope biogenesis]. // Polyadenylation factor I complex subunit FIP1 [RNA processing and modification]. // DNA polymerase epsilon catalytic subunit A [Replication recombination and repair]. // Wiskott Aldrich syndrome proteins [Cytoskeleton]. // Vesicle coat complex COPII subunit SFB3 [Intracellular trafficking secretion and vesicular transport]. // Vesicle coat complex COPII subunit SEC24/subunit SFB2 [Intracellular trafficking secretion and vesicular transport]. // Actin regulatory protein (Wiskott-Aldrich syndrome protein) [Signal transduction mechanisms Cytoskeleton]. Code Enzyme  EC:2.4.1.34
Gene Prediction Quality  validated Protein length 

Sequence

Length: 1247  
Kegg Orthology  K11000

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0 Synonyms

4 GO Terms

Identifier Name Description
GO:0003676 nucleic acid binding Interacting selectively and non-covalently with any nucleic acid.
GO:0016851 magnesium chelatase activity Catalysis of the reaction: ATP + H(2)O + Mg(2+) + protoporphyrin IX = ADP + 2 H(+) + magnesium protoporphyrin IX + phosphate.
GO:0008408 3'-5' exonuclease activity Catalysis of the hydrolysis of ester linkages within nucleic acids by removing nucleotide residues from the 3' end.
GO:0003843 1,3-beta-D-glucan synthase activity Catalysis of the reaction: UDP-glucose + [(1->3)-beta-D-glucosyl](n) = UDP + [(1->3)-beta-D-glucosyl](n+1).

40 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|mdm:103413515 204 660 + 457 Gaps:6 23.51 1952 71.68 0.0 callose synthase 1-like
blastp_kegg lcl|cic:CICLE_v10030476mg 267 659 + 393 Gaps:1 20.18 1952 79.70 0.0 hypothetical protein
blastp_kegg lcl|cit:102624514 267 659 + 393 Gaps:1 20.18 1952 79.70 0.0 callose synthase 2-like
blastp_kegg lcl|pxb:103954919 204 660 + 457 Gaps:6 23.51 1952 71.02 0.0 callose synthase 1
blastp_kegg lcl|pxb:103945817 217 660 + 444 Gaps:8 22.44 1952 73.74 0.0 callose synthase 1-like
blastp_kegg lcl|pper:PRUPE_ppa000074mg 217 646 + 430 Gaps:8 21.71 1953 75.47 0.0 hypothetical protein
blastp_kegg lcl|ath:AT2G31960 267 646 + 380 Gaps:1 19.54 1950 79.53 0.0 GSL03 glucan synthase-like 3
blastp_kegg lcl|brp:103867676 267 646 + 380 Gaps:1 19.50 1954 79.79 0.0 callose synthase 2
blastp_kegg lcl|eus:EUTSA_v10016125mg 267 646 + 380 Gaps:1 19.54 1950 79.53 0.0 hypothetical protein
blastp_kegg lcl|tcc:TCM_040369 267 646 + 380 Gaps:2 76.55 499 76.18 0.0 Glucan synthase-like 12 isoform 1
blastp_uniprot_sprot sp|Q9SL03|CALS2_ARATH 267 646 + 380 Gaps:1 19.54 1950 79.53 0.0 Callose synthase 2 OS Arabidopsis thaliana GN CALS2 PE 2 SV 3
blastp_uniprot_sprot sp|Q9AUE0|CALS1_ARATH 267 647 + 381 Gaps:1 19.59 1950 78.27 0.0 Callose synthase 1 OS Arabidopsis thaliana GN CALS1 PE 1 SV 2
blastp_uniprot_sprot sp|Q9LXT9|CALS3_ARATH 267 636 + 370 Gaps:2 19.03 1955 77.69 0.0 Callose synthase 3 OS Arabidopsis thaliana GN CALS3 PE 2 SV 3
blastp_uniprot_sprot sp|Q3B724|CALS5_ARATH 206 633 + 428 Gaps:13 22.72 1923 53.32 3e-137 Callose synthase 5 OS Arabidopsis thaliana GN CALS5 PE 1 SV 1
blastp_uniprot_sprot sp|Q9LTG5|CALS4_ARATH 291 646 + 356 Gaps:10 19.03 1871 57.87 7e-115 Callose synthase 4 OS Arabidopsis thaliana GN CALS4 PE 3 SV 2
blastp_uniprot_sprot sp|Q9LUD7|CALS8_ARATH 285 634 + 350 Gaps:17 17.16 1976 54.57 9e-104 Putative callose synthase 8 OS Arabidopsis thaliana GN CALS8 PE 2 SV 2
blastp_uniprot_sprot sp|F4HW04|DPOE1_ARATH 709 901 + 193 Gaps:1 8.98 2161 81.96 5e-94 DNA polymerase epsilon catalytic subunit A OS Arabidopsis thaliana GN POL2A PE 1 SV 1
blastp_uniprot_sprot sp|F4IFN6|DPOE2_ARATH 709 901 + 193 none 9.03 2138 78.76 2e-89 DNA polymerase epsilon catalytic subunit B OS Arabidopsis thaliana GN POL2B PE 2 SV 1
blastp_uniprot_sprot sp|Q9FNB0|CHLH_ARATH 902 1113 + 212 Gaps:8 15.21 1381 78.10 1e-86 Magnesium-chelatase subunit ChlH chloroplastic OS Arabidopsis thaliana GN CHLH PE 1 SV 1
blastp_uniprot_sprot sp|Q9LYS6|CALS6_ARATH 267 638 + 372 Gaps:21 19.63 1921 44.83 4e-85 Putative callose synthase 6 OS Arabidopsis thaliana GN CALS6 PE 3 SV 2
rpsblast_cdd gnl|CDD|178618 956 1113 + 158 Gaps:1 13.03 1220 75.47 2e-81 PLN03069 PLN03069 magnesiumprotoporphyrin-IX chelatase subunit H Provisional.
rpsblast_cdd gnl|CDD|206456 511 627 + 117 Gaps:6 100.00 113 54.87 5e-55 pfam14288 FKS1_dom1 1 3-beta-glucan synthase subunit FKS1 domain-1. The FKS1_dom1 domain is likely to be the 'Class I' region just N-terminal to the first set of transmembrane helices that is involved in 1 3-beta-glucan synthesis itself. This family is found on proteins with family Glucan_synthase pfam02364.
rpsblast_cdd gnl|CDD|183556 964 1113 + 150 Gaps:4 11.60 1310 46.05 2e-46 PRK12493 PRK12493 magnesium chelatase subunit H Provisional.
rpsblast_cdd gnl|CDD|152400 978 1115 + 138 Gaps:12 86.59 164 36.62 3e-34 pfam11965 DUF3479 Domain of unknown function (DUF3479). This presumed domain is functionally uncharacterized. This domain is found in bacteria archaea and eukaryotes. This domain is about 160 amino acids in length. This domain is found associated with pfam02514.
rpsblast_cdd gnl|CDD|99822 821 894 + 74 none 36.27 204 71.62 5e-34 cd05779 DNA_polB_epsilon_exo DEDDy 3'-5' exonuclease domain of eukaryotic DNA polymerase epsilon a family-B DNA polymerase. The 3'-5' exonuclease domain of eukaryotic DNA polymerase epsilon. DNA polymerase epsilon is a family-B DNA polymerase with a catalytic subunit that contains a DEDDy-type DnaQ-like 3'-5' exonuclease domain. It is one of the three DNA-dependent type B DNA polymerases (alpha and delta are the other two) that have been identified as essential for nuclear DNA replication in eukaryotes. DNA polymerase epsilon plays a role in elongating the leading strand during DNA replication. It is also involved in DNA repair. The catalytic subunit contains both polymerase and 3'-5' exonuclease activities. The N-terminal exonuclease domain contains three sequence motifs termed ExoI ExoII and ExoIII with a specific YX(3)D pattern at ExoIII. These motifs are clustered around the active site and are involved in metal binding and catalysis. DNA polymerase epsilon also carries a unique large C-terminal domain with an unknown function. Phylogenetic analyses indicate that it is orthologous to the archaeal DNA polymerase B3 rather than to the eukaryotic alpha delta or zeta polymerases. The exonuclease domain of family-B polymerases contains a beta hairpin structure that plays an important role in active site switching in the event of nucleotide misincorporation.
rpsblast_cdd gnl|CDD|188195 979 1141 + 163 Gaps:2 13.15 1224 28.57 3e-25 TIGR02025 BchH magnesium chelatase H subunit. This model represents the H subunit of the magnesium chelatase complex responsible for magnesium insertion into the protoporphyrin IX ring in the biosynthesis of both chlorophyll and bacteriochlorophyll. In chlorophyll-utilizing species this gene is known as ChlH while in bacteriochlorophyll-utilizing spoecies it is called BchH. Subunit H is the largest (~140kDa) of the three subunits (the others being BchD/ChlD and BchI/ChlI) and is known to bind protoporphyrin IX. Subunit H is homologous to the CobN subunit of cobaltochelatase and by anology with that enzyme subunit H is believed to also bind the magnesium ion which is inserted into the ring. In conjunction with the hydrolysis of ATP by subunits I and D a conformation change is believed to happen in subunit H causing the magnesium ion insertion into the distorted protoporphyrin ring.
rpsblast_cdd gnl|CDD|202537 778 898 + 121 Gaps:27 51.97 254 30.30 5e-20 pfam03104 DNA_pol_B_exo1 DNA polymerase family B exonuclease domain. This domain has 3' to 5' exonuclease activity and adopts a ribonuclease H type fold.
rpsblast_cdd gnl|CDD|30766 770 875 + 106 Gaps:2 13.64 792 33.33 8e-15 COG0417 PolB DNA polymerase elongation subunit (family B) [DNA replication recombination and repair].
rpsblast_cdd gnl|CDD|165506 66 288 + 223 Gaps:181 20.82 3151 41.31 2e-13 PHA03247 PHA03247 large tegument protein UL36 Provisional.
rpsblast_cdd gnl|CDD|177580 65 309 + 245 Gaps:105 28.40 1352 38.28 1e-10 PHA03307 PHA03307 transcriptional regulator ICP4 Provisional.

8 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
Gene3D 824 894 71 G3DSA:3.30.420.10 none none IPR012337
Pfam 511 626 116 PF14288 "KEGG:00500+2.4.1.34","MetaCyc:PWY-6773" 1,3-beta-glucan synthase subunit FKS1, domain-1 IPR026899
PANTHER 303 643 341 PTHR12741:SF9 "KEGG:00500+2.4.1.34","MetaCyc:PWY-6773";signature_desc=CALLOSE SYNTHASE 1-RELATED (PTHR12741:SF9) none IPR026953
Pfam 978 1109 132 PF11965 "KEGG:00860+6.6.1.1","MetaCyc:PWY-5531","MetaCyc:PWY-7159" Domain of unknown function (DUF3479) IPR022571
SUPERFAMILY 711 740 30 SSF53098 none none IPR012337
SUPERFAMILY 771 873 103 SSF53098 none none IPR012337
PANTHER 303 643 341 PTHR12741 none none none
Pfam 673 894 222 PF03104 "KEGG:00230+2.7.7.7","KEGG:00240+2.7.7.7" DNA polymerase family B, exonuclease domain IPR006133

0 Localization

0 Qtllist

0 Targeting