Protein : Qrob_P0273200.2 Q. robur
Protein Identifier  ? Qrob_P0273200.2 Organism . Name  Quercus robur
Score  14.1 Score Type  egn
Protein Description  (M=6) K01176 - alpha-amylase [EC:3.2.1.1] Code Enzyme  EC:3.2.1.1
Gene Prediction Quality  validated Protein length 

Sequence

Length: 426  
Kegg Orthology  K01176
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0 Synonyms

5 GO Terms

Identifier Name Description
GO:0003824 catalytic activity Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic.
GO:0005975 carbohydrate metabolic process The chemical reactions and pathways involving carbohydrates, any of a group of organic compounds based of the general formula Cx(H2O)y. Includes the formation of carbohydrate derivatives by the addition of a carbohydrate residue to another molecule.
GO:0005509 calcium ion binding Interacting selectively and non-covalently with calcium ions (Ca2+).
GO:0043169 cation binding Interacting selectively and non-covalently with cations, charged atoms or groups of atoms with a net positive charge.
GO:0004556 alpha-amylase activity Catalysis of the endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more alpha-(1->4)-linked D-glucose units.

42 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|mtr:MTR_1g019440 4 423 + 420 Gaps:1 100.00 421 77.43 0.0 Alpha-amylase
blastp_kegg lcl|pvu:PHAVU_010G017600g 4 423 + 420 Gaps:2 100.00 420 75.71 0.0 hypothetical protein
blastp_kegg lcl|pop:POPTR_0002s12790g 4 424 + 421 Gaps:3 100.00 422 77.73 0.0 hypothetical protein
blastp_kegg lcl|cam:101506967 10 423 + 414 Gaps:3 99.29 420 76.50 0.0 alpha-amylase-like
blastp_kegg lcl|pop:POPTR_0515s00220g 28 425 + 398 Gaps:2 99.01 404 79.25 0.0 alpha amylase family protein
blastp_kegg lcl|cam:101508459 10 425 + 416 Gaps:3 99.29 422 75.42 0.0 alpha-amylase-like
blastp_kegg lcl|pop:POPTR_0014s02940g 4 424 + 421 Gaps:4 100.00 423 75.18 0.0 alpha amylase family protein
blastp_kegg lcl|cic:CICLE_v10001266mg 4 424 + 421 Gaps:2 99.76 424 74.94 0.0 hypothetical protein
blastp_kegg lcl|cit:102612446 4 424 + 421 Gaps:2 99.76 424 74.94 0.0 alpha-amylase-like
blastp_kegg lcl|rcu:RCOM_1590650 7 425 + 419 Gaps:4 99.76 422 73.40 0.0 alpha-amylase putative (EC:3.2.1.60)
blastp_pdb 1bg9_A 28 424 + 397 Gaps:4 99.50 403 65.09 0.0 mol:protein length:403 1 4-ALPHA-D-GLUCAN GLUCANOHYDROLASE
blastp_pdb 1ava_B 28 424 + 397 Gaps:4 99.50 403 65.09 0.0 mol:protein length:403 BARLEY ALPHA-AMYLASE 2(CV MENUET)
blastp_pdb 1ava_A 28 424 + 397 Gaps:4 99.50 403 65.09 0.0 mol:protein length:403 BARLEY ALPHA-AMYLASE 2(CV MENUET)
blastp_pdb 1amy_A 28 424 + 397 Gaps:4 99.50 403 65.09 0.0 mol:protein length:403 1 4-ALPHA-D-GLUCAN GLUCANOHYDROLASE
blastp_pdb 2qpu_C 28 424 + 397 Gaps:5 99.26 405 60.95 0.0 mol:protein length:405 Alpha-amylase type A isozyme
blastp_pdb 2qpu_B 28 424 + 397 Gaps:5 99.26 405 60.95 0.0 mol:protein length:405 Alpha-amylase type A isozyme
blastp_pdb 2qpu_A 28 424 + 397 Gaps:5 99.26 405 60.95 0.0 mol:protein length:405 Alpha-amylase type A isozyme
blastp_pdb 3bsg_A 28 424 + 397 Gaps:5 97.10 414 60.70 0.0 mol:protein length:414 Alpha-amylase type A isozyme
blastp_pdb 1rpk_A 28 424 + 397 Gaps:5 99.26 405 60.70 0.0 mol:protein length:405 Alpha-amylase type 1 isozyme
blastp_pdb 1p6w_A 28 424 + 397 Gaps:5 99.26 405 60.70 0.0 mol:protein length:405 PROTEIN (Alpha-amylase type A isozyme)
blastp_uniprot_sprot sp|P17859|AMYA_VIGMU 4 423 + 420 Gaps:1 100.00 421 77.20 0.0 Alpha-amylase OS Vigna mungo GN AMY1.1 PE 2 SV 1
blastp_uniprot_sprot sp|Q8VZ56|AMY1_ARATH 3 425 + 423 Gaps:6 100.00 423 67.38 0.0 Alpha-amylase 1 OS Arabidopsis thaliana GN AMY1 PE 2 SV 1
blastp_uniprot_sprot sp|P04063|AMY2_HORVU 22 424 + 403 Gaps:4 95.32 427 64.62 0.0 Alpha-amylase type B isozyme OS Hordeum vulgare GN AMY1.2 PE 1 SV 3
blastp_uniprot_sprot sp|P04750|AMY6_HORVU 3 424 + 422 Gaps:6 99.77 429 61.21 0.0 Alpha-amylase type B isozyme OS Hordeum vulgare GN AMY1.6 PE 2 SV 2
blastp_uniprot_sprot sp|P27932|AMY3A_ORYSJ 5 424 + 420 Gaps:5 96.59 440 59.06 0.0 Alpha-amylase isozyme 3A OS Oryza sativa subsp. japonica GN AMY1.2 PE 2 SV 2
blastp_uniprot_sprot sp|P27934|AMY3E_ORYSJ 7 425 + 419 Gaps:4 95.88 437 59.90 0.0 Alpha-amylase isozyme 3E OS Oryza sativa subsp. japonica GN AMY1.4 PE 2 SV 1
blastp_uniprot_sprot sp|P27933|AMY3D_ORYSJ 26 424 + 399 Gaps:2 91.97 436 61.85 0.0 Alpha-amylase isozyme 3D OS Oryza sativa subsp. japonica GN AMY1.3 PE 2 SV 2
blastp_uniprot_sprot sp|P27939|AMY3C_ORYSJ 23 424 + 402 Gaps:2 92.45 437 62.13 0.0 Alpha-amylase isozyme 3C OS Oryza sativa subsp. japonica GN AMY1.7 PE 2 SV 2
blastp_uniprot_sprot sp|P27937|AMY3B_ORYSJ 23 424 + 402 Gaps:2 92.24 438 62.13 0.0 Alpha-amylase isozyme 3B OS Oryza sativa subsp. japonica GN AMY1.6 PE 2 SV 1
blastp_uniprot_sprot sp|P17654|AMY1_ORYSJ 19 424 + 406 Gaps:6 94.47 434 60.98 0.0 Alpha-amylase OS Oryza sativa subsp. japonica GN AMY1.1 PE 2 SV 2

19 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
SUPERFAMILY 28 366 339 SSF51445 none none IPR017853
SUPERFAMILY 368 424 57 SSF51011 none none none
Phobius 1 26 26 SIGNAL_PEPTIDE none Signal peptide region none
PRINTS 106 117 12 PR00110 "KEGG:00500+3.2.1.1","Reactome:REACT_474" Alpha-amylase signature IPR006046
PRINTS 198 209 12 PR00110 "KEGG:00500+3.2.1.1","Reactome:REACT_474" Alpha-amylase signature IPR006046
PRINTS 302 314 13 PR00110 "KEGG:00500+3.2.1.1","Reactome:REACT_474" Alpha-amylase signature IPR006046
PANTHER 25 423 399 PTHR10357:SF112 none none none
Phobius 27 425 399 NON_CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. none
Phobius 1 5 5 SIGNAL_PEPTIDE_N_REGION none N-terminal region of a signal peptide. none
SMART 27 363 337 SM00642 none Alpha-amylase domain IPR006589
Pfam 365 424 60 PF07821 "KEGG:00500+3.2.1.1" Alpha-amylase C-terminal beta-sheet domain IPR012850
Phobius 6 17 12 SIGNAL_PEPTIDE_H_REGION none Hydrophobic region of a signal peptide. none
Gene3D 29 367 339 G3DSA:3.20.20.80 none none IPR013781
PANTHER 25 423 399 PTHR10357 none none IPR015902
SMART 364 424 61 SM00810 "KEGG:00500+3.2.1.1" Alpha-amylase C-terminal beta-sheet domain IPR012850
Pfam 47 344 298 PF00128 none Alpha amylase, catalytic domain IPR006047
PIRSF 1 425 425 PIRSF001028 "KEGG:00500+3.2.1.1" none IPR013775
Gene3D 368 425 58 G3DSA:2.60.40.1180 none none IPR013780
Phobius 18 26 9 SIGNAL_PEPTIDE_C_REGION none C-terminal region of a signal peptide. none

3 Localization

Analysis Start End Length
TMHMM 7 29 22
SignalP_EUK 1 18 17
SignalP_GRAM_POSITIVE 1 24 23

11 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Bourran1_2003_QTL2_peak_Bud_burst_A4 Qrob_Chr02 2 s_1B0H8U_259 s_1CB1VL_554 17 0 87 lod 3,3 8,7
Bourran1_2004_QTL2_peak_Bud_burst_3P Qrob_Chr02 2 s_1AW12F_382 s_1A77MR_223 42 6 64 lod 3,6 9,6
Bourran2_2003_QTL8_peak_Bud_burst_3P Qrob_Chr02 2 s_1ANG6_1446 v_11270_161 40 0 72 lod 4,4 9,9
Bourran2_2014_nP_A4 Qrob_Chr11 11 s_1B58GB_1413 s_1A5BYY_1671 11,15 0 42,38 lod 1,8913 4,5
Bourran2_2015_nPriLBD_A4 Qrob_Chr02 2 s_1CP5DI_1183 s_1A63ZX_1277 24,87 24,63 26,18 lod 3.8 7
Bourran1_2003_QTL1_peak_Bud_burst_3P Qrob_Chr02 2 s_1AR8KI_1183 s_1B0QB1_473 22 6 41 lod 4,2 11,5
Bourran1_2004_QTL3_peak_Bud_burst_A4 Qrob_Chr02 2 s_1B0H8U_259 s_1CB1VL_554 17 0 46 lod 2,9 6,4
Bourran2_2015_nEpiBC_A4 Qrob_Chr07 7 s_1DP9TW_798 v_8128_173 22,61 22,14 22,73 lod 3.1 8.5
Champenoux_2015_nP_A4 Qrob_Chr02 2 s_1BN4CB_644 v_508_128 23,76 23,06 24,51 lod 2.8 6.2
Champenoux_2015_nPriLBD_A4 Qrob_Chr02 2 s_1CP5DI_1183 s_1A63ZX_1277 25,35 24,63 26,18 lod 4.0 8.7
Champenoux_2015_nSecLBD_A4 Qrob_Chr02 2 s_1AN4ZM_1665 v_8587_238 18,52 18,89 18,27 lod 3.2 7.4

1 Targeting

Analysis Start End Length Location Reliability Signal Peptide Cut Off Mitochondrion Cut Off Network Signal Peptide Length
TargetP 1 26   Secretory pathway 1 0.991 0.011 NON-PLANT 26