Protein : Qrob_P0261400.2 Q. robur
Protein Identifier  ? Qrob_P0261400.2 Organism . Name  Quercus robur
Score  0.0 Score Type  egn
Protein Description  (M=2) 1.3.1.88 - tRNA-dihydrouridine(16/17) synthase (NAD(P)(+)). Code Enzyme  EC:1.3.1.88
Gene Prediction Quality  validated Protein length 

Sequence

Length: 370  
Kegg Orthology  K05542
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0 Synonyms

5 GO Terms

Identifier Name Description
GO:0055114 oxidation-reduction process A metabolic process that results in the removal or addition of one or more electrons to or from a substance, with or without the concomitant removal or addition of a proton or protons.
GO:0003824 catalytic activity Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic.
GO:0050660 flavin adenine dinucleotide binding Interacting selectively and non-covalently with FAD, flavin-adenine dinucleotide, the coenzyme or the prosthetic group of various flavoprotein oxidoreductase enzymes, in either the oxidized form, FAD, or the reduced form, FADH2.
GO:0008033 tRNA processing The process in which a pre-tRNA molecule is converted to a mature tRNA, ready for addition of an aminoacyl group.
GO:0017150 tRNA dihydrouridine synthase activity Catalysis of the reaction: tRNA-uracil + acceptor = tRNA-dihydrouridine + reduced acceptor.

29 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|cit:102626482 126 366 + 241 Gaps:27 61.47 436 61.94 5e-110 tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like
blastp_kegg lcl|cic:CICLE_v10025630mg 126 366 + 241 Gaps:27 61.47 436 62.31 5e-110 hypothetical protein
blastp_kegg lcl|vvi:100249291 126 366 + 241 Gaps:28 62.97 424 62.92 1e-109 tRNA-dihydrouridine synthase 1-like
blastp_kegg lcl|cmo:103501114 126 363 + 238 Gaps:27 62.21 426 61.89 3e-109 tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like
blastp_kegg lcl|rcu:RCOM_0550920 126 365 + 240 Gaps:29 63.15 426 62.08 1e-108 tRNA-dihydrouridine synthase putative
blastp_kegg lcl|pper:PRUPE_ppa006605mg 126 366 + 241 Gaps:27 66.50 403 61.57 2e-108 hypothetical protein
blastp_kegg lcl|csv:101209488 126 363 + 238 Gaps:27 62.21 426 61.51 4e-108 tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like
blastp_kegg lcl|pmum:103337538 126 366 + 241 Gaps:27 62.76 427 60.82 5e-107 tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like
blastp_kegg lcl|tcc:TCM_000679 126 354 + 229 Gaps:27 58.99 434 63.28 6e-106 FMN-linked oxidoreductases superfamily protein
blastp_kegg lcl|pop:POPTR_0007s10680g 126 365 + 240 Gaps:14 62.87 404 65.35 2e-105 POPTRDRAFT_719209 hypothetical protein
blastp_uniprot_sprot sp|Q8K582|DUS1L_RAT 126 322 + 197 Gaps:11 47.49 438 37.98 1e-39 tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS Rattus norvegicus GN Dus1l PE 2 SV 1
blastp_uniprot_sprot sp|Q6P1R4|DUS1L_HUMAN 126 322 + 197 Gaps:11 43.97 473 38.46 2e-39 tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS Homo sapiens GN DUS1L PE 1 SV 1
blastp_uniprot_sprot sp|Q8C2P3|DUS1L_MOUSE 126 322 + 197 Gaps:11 43.79 475 37.50 7e-39 tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS Mus musculus GN Dus1l PE 2 SV 1
blastp_uniprot_sprot sp|Q9HGN6|DUS1_SCHPO 126 276 + 151 Gaps:9 40.10 399 39.38 1e-27 tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)] OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN dus1 PE 3 SV 1
blastp_uniprot_sprot sp|P53759|DUS1_YEAST 155 276 + 122 Gaps:2 29.31 423 41.94 3e-24 tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)] OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN DUS1 PE 1 SV 1
blastp_uniprot_sprot sp|Q9LZS0|PTL_ARATH 1 145 + 145 Gaps:23 27.07 591 45.00 7e-24 Trihelix transcription factor PTL OS Arabidopsis thaliana GN PTL PE 2 SV 1
blastp_uniprot_sprot sp|O95620|DUS4L_HUMAN 162 279 + 118 Gaps:6 37.22 317 38.98 5e-19 tRNA-dihydrouridine(20a/20b) synthase [NAD(P)+]-like OS Homo sapiens GN DUS4L PE 2 SV 2
blastp_uniprot_sprot sp|Q32M08|DUS4L_MOUSE 162 279 + 118 Gaps:6 36.42 324 39.83 1e-18 tRNA-dihydrouridine(20a/20b) synthase [NAD(P)+]-like OS Mus musculus GN Dus4l PE 2 SV 1
blastp_uniprot_sprot sp|O74553|DUS4_SCHPO 180 284 + 105 Gaps:2 31.60 326 39.81 1e-16 tRNA-dihydrouridine(20a/20b) synthase [NAD(P)+] OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN dus4 PE 3 SV 1
blastp_uniprot_sprot sp|Q09504|YQI2_CAEEL 178 338 + 161 Gaps:29 57.04 284 36.42 1e-16 Uncharacterized tRNA-dihydrouridine synthase-like protein C45G9.2 OS Caenorhabditis elegans GN C45G9.2 PE 3 SV 2
rpsblast_cdd gnl|CDD|73368 147 284 + 138 Gaps:4 58.87 231 38.24 7e-34 cd02801 DUS_like_FMN Dihydrouridine synthase-like (DUS-like) FMN-binding domain. Members of this family catalyze the reduction of the 5 6-double bond of a uridine residue on tRNA. Dihydrouridine modification of tRNA is widely observed in prokaryotes and eukaryotes and also in some archaea. Most dihydrouridines are found in the D loop of t-RNAs. The role of dihydrouridine in tRNA is currently unknown but may increase conformational flexibility of the tRNA. It is likely that different family members have different substrate specificities which may overlap. 1VHN a putative flavin oxidoreductase has high sequence similarity to DUS. The enzymatic mechanism of 1VHN is not known at the present..
rpsblast_cdd gnl|CDD|144707 162 284 + 123 Gaps:4 39.81 309 39.84 9e-30 pfam01207 Dus Dihydrouridine synthase (Dus). Members of this family catalyze the reduction of the 5 6-double bond of a uridine residue on tRNA. Dihydrouridine modification of tRNA is widely observed in prokaryotes and eukaryotes and also in some archae. Most dihydrouridines are found in the D loop of t-RNAs. The role of dihydrouridine in tRNA is currently unknown but may increase conformational flexibility of the tRNA. It is likely that different family members have different substrate specificities which may overlap. Dus 1 from Saccharomyces cerevisiae acts on pre-tRNA-Phe while Dus 2 acts on pre-tRNA-Tyr and pre-tRNA-Leu. Dus 1 is active as a single subunit requiring NADPH or NADH and is stimulated by the presence of FAD. Some family members may be targeted to the mitochondria and even have a role in mitochondria.
rpsblast_cdd gnl|CDD|30391 156 320 + 165 Gaps:16 51.70 323 32.34 7e-24 COG0042 COG0042 tRNA-dihydrouridine synthase [Translation ribosomal structure and biogenesis].
rpsblast_cdd gnl|CDD|129820 162 277 + 116 Gaps:6 36.36 319 37.93 5e-18 TIGR00737 nifR3_yhdG putative TIM-barrel protein nifR3 family. This model represents one branch of COG0042 (Predicted TIM-barrel enzymes possibly dehydrogenases nifR3 family). This branch includes NifR3 itself from Rhodobacter capsulatus. It excludes a broadly distributed but more sparsely populated subfamily that contains sll0926 from Synechocystis PCC6803 HI0634 from Haemophilus influenzae and BB0225 from Borrelia burgdorferi. It also excludes a shorter and more distant archaeal subfamily.The function of nifR3 a member of this family is unknown but it is found in an operon with nitrogen-sensing two component regulators in Rhodobacter capsulatus.Members of this family show a distant relationship to alpha/beta (TIM) barrel enzymes such as dihydroorotate dehydrogenase and glycolate oxidase.
rpsblast_cdd gnl|CDD|182540 171 275 + 105 Gaps:8 33.65 312 35.24 4e-12 PRK10550 PRK10550 tRNA-dihydrouridine synthase C Provisional.
rpsblast_cdd gnl|CDD|182440 145 276 + 132 Gaps:9 40.81 321 34.35 2e-10 PRK10415 PRK10415 tRNA-dihydrouridine synthase B Provisional.
rpsblast_cdd gnl|CDD|73369 177 282 + 106 Gaps:12 36.09 327 26.27 5e-08 cd02803 OYE_like_FMN_family Old yellow enzyme (OYE)-like FMN binding domain. OYE was the first flavin-dependent enzyme identified however its true physiological role remains elusive to this day. Each monomer of OYE contains FMN as a non-covalently bound cofactor uses NADPH as a reducing agent with oxygens quinones and alpha beta-unsaturated aldehydes and ketones and can act as electron acceptors in the catalytic reaction. Members of OYE family include trimethylamine dehydrogenase 2 4-dienoyl-CoA reductase enoate reductase pentaerythriol tetranitrate reductase xenobiotic reductase and morphinone reductase..
rpsblast_kog gnl|CDD|37546 154 350 + 197 Gaps:11 58.10 358 42.79 1e-53 KOG2335 KOG2335 KOG2335 tRNA-dihydrouridine synthase [Translation ribosomal structure and biogenesis].
rpsblast_kog gnl|CDD|37545 148 276 + 129 Gaps:7 27.25 477 33.85 6e-15 KOG2334 KOG2334 KOG2334 tRNA-dihydrouridine synthase [Translation ribosomal structure and biogenesis].

5 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
PANTHER 155 342 188 PTHR11082 none none IPR001269
PANTHER 155 342 188 PTHR11082:SF5 none none none
SUPERFAMILY 155 309 155 SSF51395 none none none
Gene3D 155 283 129 G3DSA:3.20.20.70 none none IPR013785
Pfam 155 284 130 PF01207 none Dihydrouridine synthase (Dus) IPR001269

0 Localization

6 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Bourran2_2014_nEpis*_A4 Qrob_Chr07 7 v_12400_446 s_1BPEBU_1211 6,93 0 15,13 lod 4,7411 11
Bourran2_2014_nLBD_A4 Qrob_Chr06 6 v_12930_125 s_1AMZEI_909 37,41 9,8 50,1 lod 1,9524 4,1
Bourran2_2014_nPriBD_A4 Qrob_Chr06 6 v_12930_125 s_1AMZEI_909 34,51 3,36 51,86 lod 1,6747 3,9
Bourran2_2014_nSecLBD_A4 Qrob_Chr07 7 v_8327_222 s_1A4WGY_363 16,04 0 44,69 lod 2,6373 6,5
Bourran2_2014_vEpiBC_A4 Qrob_Chr06 6 v_12930_125 s_1AMZEI_909 37,55 14,41 50,01 lod 1,7882 4,8
Champenoux_2015_nEpis_3P Qrob_Chr11 11 s_1DG9PM_867 s_1BZ083_1312 26,53 25,47 27,72 lod 4.4 8.9

1 Targeting

Analysis Start End Length Location Reliability Signal Peptide Cut Off Mitochondrion Cut Off Network Signal Peptide Length
TargetP 1 24   Mitochondrion 5 0.055 0.516 NON-PLANT 24