Protein : Qrob_P0261290.2 Q. robur
Protein Identifier  ? Qrob_P0261290.2 Organism . Name  Quercus robur
Score  100.0 Score Type  egn
Protein Description  (M=2) 1.3.1.88 - tRNA-dihydrouridine(16/17) synthase (NAD(P)(+)). Code Enzyme  EC:1.3.1.88
Gene Prediction Quality  validated Protein length 

Sequence

Length: 405  
Kegg Orthology  K05542
Paste the following link
Lists
This Protein isn't in any lists. Upload a list.

Sequence Feature Displayer

Protein Sequence Displayer

J Browse Displayer

0 Synonyms

5 GO Terms

Identifier Name Description
GO:0055114 oxidation-reduction process A metabolic process that results in the removal or addition of one or more electrons to or from a substance, with or without the concomitant removal or addition of a proton or protons.
GO:0003824 catalytic activity Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic.
GO:0050660 flavin adenine dinucleotide binding Interacting selectively and non-covalently with FAD, flavin-adenine dinucleotide, the coenzyme or the prosthetic group of various flavoprotein oxidoreductase enzymes, in either the oxidized form, FAD, or the reduced form, FADH2.
GO:0008033 tRNA processing The process in which a pre-tRNA molecule is converted to a mature tRNA, ready for addition of an aminoacyl group.
GO:0017150 tRNA dihydrouridine synthase activity Catalysis of the reaction: tRNA-uracil + acceptor = tRNA-dihydrouridine + reduced acceptor.

39 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|cmo:103501114 1 398 + 398 Gaps:7 95.07 426 76.05 0.0 tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like
blastp_kegg lcl|vvi:100249291 1 401 + 401 Gaps:13 94.34 424 78.00 0.0 tRNA-dihydrouridine synthase 1-like
blastp_kegg lcl|pper:PRUPE_ppa006605mg 1 401 + 401 Gaps:8 100.00 403 76.43 0.0 hypothetical protein
blastp_kegg lcl|cit:102626482 4 401 + 398 Gaps:13 94.27 436 75.18 0.0 tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like
blastp_kegg lcl|tcc:TCM_000679 3 389 + 387 Gaps:11 91.71 434 77.14 0.0 FMN-linked oxidoreductases superfamily protein
blastp_kegg lcl|csv:101209488 1 398 + 398 Gaps:9 95.07 426 75.06 0.0 tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like
blastp_kegg lcl|cic:CICLE_v10025630mg 4 401 + 398 Gaps:13 94.27 436 74.94 0.0 hypothetical protein
blastp_kegg lcl|rcu:RCOM_0550920 1 399 + 399 Gaps:8 95.07 426 76.54 0.0 tRNA-dihydrouridine synthase putative
blastp_kegg lcl|pmum:103337538 1 401 + 401 Gaps:14 94.38 427 75.93 0.0 tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like
blastp_kegg lcl|mdm:103401401 2 401 + 400 Gaps:3 92.79 430 74.44 0.0 tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like
blastp_pdb 3b0u_Y 68 288 + 221 Gaps:30 65.43 350 31.00 8e-22 mol:protein length:350 tRNA-dihydrouridine synthase
blastp_pdb 3b0u_X 68 288 + 221 Gaps:30 65.43 350 31.00 8e-22 mol:protein length:350 tRNA-dihydrouridine synthase
blastp_pdb 3b0p_B 68 288 + 221 Gaps:30 65.43 350 31.00 8e-22 mol:protein length:350 tRNA-dihydrouridine synthase
blastp_pdb 3b0p_A 68 288 + 221 Gaps:30 65.43 350 31.00 8e-22 mol:protein length:350 tRNA-dihydrouridine synthase
blastp_pdb 3b0v_D 68 288 + 221 Gaps:30 63.09 363 31.00 9e-22 mol:protein length:363 tRNA-dihydrouridine synthase
blastp_pdb 3b0v_C 68 288 + 221 Gaps:30 63.09 363 31.00 9e-22 mol:protein length:363 tRNA-dihydrouridine synthase
blastp_pdb 1vhn_A 69 291 + 223 Gaps:7 68.55 318 30.28 3e-21 mol:protein length:318 putative flavin oxidoreducatase
blastp_uniprot_sprot sp|Q8K582|DUS1L_RAT 65 357 + 293 Gaps:18 63.70 438 47.67 7e-87 tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS Rattus norvegicus GN Dus1l PE 2 SV 1
blastp_uniprot_sprot sp|Q8C2P3|DUS1L_MOUSE 65 357 + 293 Gaps:18 58.74 475 47.31 1e-86 tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS Mus musculus GN Dus1l PE 2 SV 1
blastp_uniprot_sprot sp|Q6P1R4|DUS1L_HUMAN 59 357 + 299 Gaps:20 59.83 473 47.70 2e-86 tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS Homo sapiens GN DUS1L PE 1 SV 1
blastp_uniprot_sprot sp|Q9HGN6|DUS1_SCHPO 64 377 + 314 Gaps:19 78.95 399 43.49 2e-80 tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)] OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN dus1 PE 3 SV 1
blastp_uniprot_sprot sp|P53759|DUS1_YEAST 44 366 + 323 Gaps:26 74.94 423 43.22 2e-76 tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)] OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN DUS1 PE 1 SV 1
blastp_uniprot_sprot sp|O95620|DUS4L_HUMAN 71 294 + 224 Gaps:6 70.66 317 43.75 6e-54 tRNA-dihydrouridine(20a/20b) synthase [NAD(P)+]-like OS Homo sapiens GN DUS4L PE 2 SV 2
blastp_uniprot_sprot sp|Q32M08|DUS4L_MOUSE 71 294 + 224 Gaps:6 69.14 324 43.75 2e-52 tRNA-dihydrouridine(20a/20b) synthase [NAD(P)+]-like OS Mus musculus GN Dus4l PE 2 SV 1
blastp_uniprot_sprot sp|Q09504|YQI2_CAEEL 75 373 + 299 Gaps:42 99.65 284 35.34 1e-45 Uncharacterized tRNA-dihydrouridine synthase-like protein C45G9.2 OS Caenorhabditis elegans GN C45G9.2 PE 3 SV 2
blastp_uniprot_sprot sp|O74553|DUS4_SCHPO 67 299 + 233 Gaps:5 71.17 326 40.09 4e-44 tRNA-dihydrouridine(20a/20b) synthase [NAD(P)+] OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN dus4 PE 3 SV 1
blastp_uniprot_sprot sp|Q06063|DUS4_YEAST 66 299 + 234 Gaps:7 64.58 367 37.13 2e-42 tRNA-dihydrouridine(20a/20b) synthase [NAD(P)+] OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN DUS4 PE 1 SV 1
rpsblast_cdd gnl|CDD|73368 69 299 + 231 Gaps:4 100.00 231 41.99 8e-74 cd02801 DUS_like_FMN Dihydrouridine synthase-like (DUS-like) FMN-binding domain. Members of this family catalyze the reduction of the 5 6-double bond of a uridine residue on tRNA. Dihydrouridine modification of tRNA is widely observed in prokaryotes and eukaryotes and also in some archaea. Most dihydrouridines are found in the D loop of t-RNAs. The role of dihydrouridine in tRNA is currently unknown but may increase conformational flexibility of the tRNA. It is likely that different family members have different substrate specificities which may overlap. 1VHN a putative flavin oxidoreductase has high sequence similarity to DUS. The enzymatic mechanism of 1VHN is not known at the present..
rpsblast_cdd gnl|CDD|144707 71 327 + 257 Gaps:16 80.58 309 38.55 2e-61 pfam01207 Dus Dihydrouridine synthase (Dus). Members of this family catalyze the reduction of the 5 6-double bond of a uridine residue on tRNA. Dihydrouridine modification of tRNA is widely observed in prokaryotes and eukaryotes and also in some archae. Most dihydrouridines are found in the D loop of t-RNAs. The role of dihydrouridine in tRNA is currently unknown but may increase conformational flexibility of the tRNA. It is likely that different family members have different substrate specificities which may overlap. Dus 1 from Saccharomyces cerevisiae acts on pre-tRNA-Phe while Dus 2 acts on pre-tRNA-Tyr and pre-tRNA-Leu. Dus 1 is active as a single subunit requiring NADPH or NADH and is stimulated by the presence of FAD. Some family members may be targeted to the mitochondria and even have a role in mitochondria.
rpsblast_cdd gnl|CDD|30391 62 369 + 308 Gaps:19 97.52 323 32.70 3e-55 COG0042 COG0042 tRNA-dihydrouridine synthase [Translation ribosomal structure and biogenesis].

6 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
Pfam 71 299 229 PF01207 none Dihydrouridine synthase (Dus) IPR001269
ProSitePatterns 151 169 19 PS01136 none Uncharacterized protein family UPF0034 signature. IPR018517
Gene3D 67 298 232 G3DSA:3.20.20.70 none none IPR013785
PANTHER 55 377 323 PTHR11082:SF5 none none none
SUPERFAMILY 68 345 278 SSF51395 none none none
PANTHER 55 377 323 PTHR11082 none none IPR001269

0 Localization

6 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Bourran2_2014_nEpis*_A4 Qrob_Chr07 7 v_12400_446 s_1BPEBU_1211 6,93 0 15,13 lod 4,7411 11
Bourran2_2014_nLBD_A4 Qrob_Chr06 6 v_12930_125 s_1AMZEI_909 37,41 9,8 50,1 lod 1,9524 4,1
Bourran2_2014_nPriBD_A4 Qrob_Chr06 6 v_12930_125 s_1AMZEI_909 34,51 3,36 51,86 lod 1,6747 3,9
Bourran2_2014_nSecLBD_A4 Qrob_Chr07 7 v_8327_222 s_1A4WGY_363 16,04 0 44,69 lod 2,6373 6,5
Bourran2_2014_vEpiBC_A4 Qrob_Chr06 6 v_12930_125 s_1AMZEI_909 37,55 14,41 50,01 lod 1,7882 4,8
Champenoux_2015_nEpis_3P Qrob_Chr11 11 s_1DG9PM_867 s_1BZ083_1312 26,53 25,47 27,72 lod 4.4 8.9

0 Targeting