Protein : Qrob_P0204580.2 Q. robur
Protein Identifier  ? Qrob_P0204580.2 Organism . Name  Quercus robur
Score  87.4 Score Type  egn
Protein Description  (M=1) PTHR10357:SF118 - ALPHA-AMYLASE 2-RELATED (PTHR10357:SF118) Code Enzyme  EC:3.2.1.1
Gene Prediction Quality  validated Protein length 

Sequence

Length: 488  
Kegg Orthology  K01176
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0 Synonyms

5 GO Terms

Identifier Name Description
GO:0003824 catalytic activity Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic.
GO:0005975 carbohydrate metabolic process The chemical reactions and pathways involving carbohydrates, any of a group of organic compounds based of the general formula Cx(H2O)y. Includes the formation of carbohydrate derivatives by the addition of a carbohydrate residue to another molecule.
GO:0005509 calcium ion binding Interacting selectively and non-covalently with calcium ions (Ca2+).
GO:0043169 cation binding Interacting selectively and non-covalently with cations, charged atoms or groups of atoms with a net positive charge.
GO:0004556 alpha-amylase activity Catalysis of the endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more alpha-(1->4)-linked D-glucose units.

41 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|pper:PRUPE_ppa006402mg 94 487 + 394 none 95.40 413 87.82 0.0 hypothetical protein
blastp_kegg lcl|pmum:103321179 94 487 + 394 none 95.40 413 87.31 0.0 probable alpha-amylase 2
blastp_kegg lcl|mdm:103413819 94 487 + 394 none 95.17 414 85.53 0.0 probable alpha-amylase 2
blastp_kegg lcl|pxb:103927258 94 487 + 394 none 95.17 414 85.79 0.0 probable alpha-amylase 2
blastp_kegg lcl|cic:CICLE_v100013481m 103 487 + 385 none 97.47 395 86.75 0.0 hypothetical protein
blastp_kegg lcl|cit:102625798 103 487 + 385 none 93.22 413 86.75 0.0 probable alpha-amylase 2-like
blastp_kegg lcl|mdm:103440221 105 487 + 383 none 92.74 413 86.42 0.0 probable alpha-amylase 2 (EC:3.2.1.1)
blastp_kegg lcl|pop:POPTR_0002s01570g 105 487 + 383 none 93.41 410 85.38 0.0 POPTRDRAFT_798380 hypothetical protein
blastp_kegg lcl|fve:101294133 105 487 + 383 none 92.29 415 85.90 0.0 probable alpha-amylase 2-like
blastp_kegg lcl|rcu:RCOM_1599860 105 487 + 383 none 96.23 398 84.33 0.0 alpha-amylase putative (EC:3.2.1.60)
blastp_pdb 3bsg_A 103 487 + 385 Gaps:23 96.62 414 46.50 2e-119 mol:protein length:414 Alpha-amylase type A isozyme
blastp_pdb 1bg9_A 103 487 + 385 Gaps:22 99.01 403 47.62 5e-119 mol:protein length:403 1 4-ALPHA-D-GLUCAN GLUCANOHYDROLASE
blastp_pdb 1ava_B 103 487 + 385 Gaps:22 99.01 403 47.62 5e-119 mol:protein length:403 BARLEY ALPHA-AMYLASE 2(CV MENUET)
blastp_pdb 1ava_A 103 487 + 385 Gaps:22 99.01 403 47.62 5e-119 mol:protein length:403 BARLEY ALPHA-AMYLASE 2(CV MENUET)
blastp_pdb 1amy_A 103 487 + 385 Gaps:22 99.01 403 47.62 5e-119 mol:protein length:403 1 4-ALPHA-D-GLUCAN GLUCANOHYDROLASE
blastp_pdb 2qps_A 103 487 + 385 Gaps:27 98.77 405 47.00 7e-119 mol:protein length:405 Alpha-amylase type A isozyme
blastp_pdb 1rpk_A 103 487 + 385 Gaps:27 98.77 405 47.00 8e-119 mol:protein length:405 Alpha-amylase type 1 isozyme
blastp_pdb 1p6w_A 103 487 + 385 Gaps:27 98.77 405 47.00 8e-119 mol:protein length:405 PROTEIN (Alpha-amylase type A isozyme)
blastp_pdb 1ht6_A 103 487 + 385 Gaps:27 98.77 405 47.00 8e-119 mol:protein length:405 ALPHA-AMYLASE ISOZYME 1
blastp_pdb 2qpu_C 103 487 + 385 Gaps:27 98.77 405 47.00 1e-118 mol:protein length:405 Alpha-amylase type A isozyme
blastp_uniprot_sprot sp|Q8LFG1|AMY2_ARATH 105 487 + 383 none 92.74 413 82.25 0.0 Probable alpha-amylase 2 OS Arabidopsis thaliana GN AMY2 PE 2 SV 1
blastp_uniprot_sprot sp|Q94A41|AMY3_ARATH 106 486 + 381 Gaps:11 43.29 887 50.26 6e-130 Alpha-amylase 3 chloroplastic OS Arabidopsis thaliana GN AMY3 PE 2 SV 1
blastp_uniprot_sprot sp|P17859|AMYA_VIGMU 103 486 + 384 Gaps:19 93.82 421 45.57 9e-119 Alpha-amylase OS Vigna mungo GN AMY1.1 PE 2 SV 1
blastp_uniprot_sprot sp|P04063|AMY2_HORVU 103 487 + 385 Gaps:22 93.44 427 47.62 3e-118 Alpha-amylase type B isozyme OS Hordeum vulgare GN AMY1.2 PE 1 SV 3
blastp_uniprot_sprot sp|Q0D9J1|AMY2A_ORYSJ 103 487 + 385 Gaps:25 90.34 445 47.76 5e-118 Alpha-amylase isozyme 2A OS Oryza sativa subsp. japonica GN AMY2A PE 2 SV 1
blastp_uniprot_sprot sp|A2YGY2|AMY2A_ORYSI 103 487 + 385 Gaps:25 90.13 446 47.76 7e-118 Alpha-amylase isozyme 2A OS Oryza sativa subsp. indica GN AMYC2 PE 2 SV 1
blastp_uniprot_sprot sp|P27934|AMY3E_ORYSJ 83 487 + 405 Gaps:22 94.97 437 45.78 8e-118 Alpha-amylase isozyme 3E OS Oryza sativa subsp. japonica GN AMY1.4 PE 2 SV 1
blastp_uniprot_sprot sp|P00693|AMY1_HORVU 103 487 + 385 Gaps:27 91.32 438 46.75 2e-117 Alpha-amylase type A isozyme OS Hordeum vulgare GN AMY1.1 PE 1 SV 1
blastp_uniprot_sprot sp|P27932|AMY3A_ORYSJ 82 487 + 406 Gaps:24 95.91 440 43.84 3e-117 Alpha-amylase isozyme 3A OS Oryza sativa subsp. japonica GN AMY1.2 PE 2 SV 2
blastp_uniprot_sprot sp|P17654|AMY1_ORYSJ 103 487 + 385 Gaps:22 91.94 434 47.37 4e-116 Alpha-amylase OS Oryza sativa subsp. japonica GN AMY1.1 PE 2 SV 2

13 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
PRINTS 364 376 13 PR00110 "KEGG:00500+3.2.1.1","Reactome:REACT_474" Alpha-amylase signature IPR006046
PRINTS 177 188 12 PR00110 "KEGG:00500+3.2.1.1","Reactome:REACT_474" Alpha-amylase signature IPR006046
PRINTS 261 272 12 PR00110 "KEGG:00500+3.2.1.1","Reactome:REACT_474" Alpha-amylase signature IPR006046
SUPERFAMILY 108 419 312 SSF51445 none none IPR017853
Gene3D 104 433 330 G3DSA:3.20.20.80 none none IPR013781
Pfam 429 487 59 PF07821 "KEGG:00500+3.2.1.1" Alpha-amylase C-terminal beta-sheet domain IPR012850
Pfam 118 407 290 PF00128 none Alpha amylase, catalytic domain IPR006047
SMART 105 440 336 SM00642 none Alpha-amylase domain IPR006589
PANTHER 105 485 381 PTHR10357 none none IPR015902
PANTHER 105 485 381 PTHR10357:SF118 none none none
Gene3D 434 487 54 G3DSA:2.60.40.1180 none none IPR013780
SUPERFAMILY 433 487 55 SSF51011 none none none
SMART 429 487 59 SM00810 "KEGG:00500+3.2.1.1" Alpha-amylase C-terminal beta-sheet domain IPR012850

0 Localization

15 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Bourran1_2003_QTL2_peak_Bud_burst_A4 Qrob_Chr02 2 s_1B0H8U_259 s_1CB1VL_554 17 0 87 lod 3,3 8,7
NancyGreenhouseCO2_2001_ambient_elevated_leaf_cellulose_QTL2_d13Cf Qrob_Chr02 2 s_1AQA4Z_1644 s_1AK5QX_947 53.67 14,01 79,68 lod 5.6594 0.03
Bourran1_2004_QTL2_peak_Bud_burst_3P Qrob_Chr02 2 s_1AW12F_382 s_1A77MR_223 42 6 64 lod 3,6 9,6
Bourran2_2002_QTL9_peak_Bud_burst_A4 Qrob_Chr02 2 s_1BFNDA_375 s_1A3VA1_2139 32,5 17 62 lod 3,1 4,2
Bourran2_2003_QTL8_peak_Bud_burst_3P Qrob_Chr02 2 s_1ANG6_1446 v_11270_161 40 0 72 lod 4,4 9,9
Bourran2_2014_nP_A4 Qrob_Chr11 11 s_1B58GB_1413 s_1A5BYY_1671 11,15 0 42,38 lod 1,8913 4,5
Bourran2_2015_nPriLBD_A4 Qrob_Chr02 2 s_1CP5DI_1183 s_1A63ZX_1277 24,87 24,63 26,18 lod 3.8 7
Bourran2_2015_nEpis_A4 Qrob_Chr09 9 v_15847_485 v_8329_369 34,94 34,88 37,45 lod 3.1 7
Bourran1_2003_QTL1_peak_Bud_burst_3P Qrob_Chr02 2 s_1AR8KI_1183 s_1B0QB1_473 22 6 41 lod 4,2 11,5
Bourran1_2004_QTL3_peak_Bud_burst_A4 Qrob_Chr02 2 s_1B0H8U_259 s_1CB1VL_554 17 0 46 lod 2,9 6,4
Bourran2_2015_nEpiBC_A4 Qrob_Chr07 7 s_1DP9TW_798 v_8128_173 22,61 22,14 22,73 lod 3.1 8.5
Champenoux_2015_nEpis_A4 Qrob_Chr02 2 s_1BAGIZ_823 s_1BN4CB_644 23,06 23,06 23,06 lod 4.9 11
Champenoux_2015_nP_A4 Qrob_Chr02 2 s_1BN4CB_644 v_508_128 23,76 23,06 24,51 lod 2.8 6.2
Champenoux_2015_nPriLBD_A4 Qrob_Chr02 2 s_1CP5DI_1183 s_1A63ZX_1277 25,35 24,63 26,18 lod 4.0 8.7
Champenoux_2015_nSecLBD_A4 Qrob_Chr02 2 s_1AN4ZM_1665 v_8587_238 18,52 18,89 18,27 lod 3.2 7.4

1 Targeting

Analysis Start End Length Location Reliability Signal Peptide Cut Off Mitochondrion Cut Off Network Signal Peptide Length
TargetP 1 37   Mitochondrion 3 0.179 0.718 NON-PLANT 37